IMMUNE NETWORK

The Korean Association of Immunobiologists (대한면역학회)
권호 표지

Expression of Gpnmb in NK Cell Development from Hematopoietic Stem Cells

  • Shin, Na-Ra (Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Ji-Won (Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Ji-Won (Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jeong, Mi-Ra (Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Mi-Sun (Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Suk-Hyung (Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Yoon, Suk-Ran (Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Chung, Jin-Woong (Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Tae-Don (Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Choi, In-Pyo (Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2008.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Background: Molecular mechanisms of natural killer (NK) cell development from hematopoietic stem cells (HSCs) have not been clearly elucidated, although the roles of some genes in NK cell development have been reported previously. Thus, searching for molecules and genes related NK cell developmental stage is important to understand the molecular events of NK cell development. Methods: From our previous SAGE data-base, Gpnmb (Glycoprotein non-metastatic melanoma protein B) was selected for further analysis. We confirmed the level of mRNA and protein of Gpnmb through RT-PCR, quantitative PCR, and FACS analysis. Then we performed cell-based ELISA and FACS analysis, to know whether there are some molecules which can bind to Gpnmb. Using neutralizing antibody, we blocked the interaction between NK cells and OP9 cells, and checked IFN-${\gamma}$ production by ELISA kit. Results: Gpnmb expression was elevated during in vitro developmental stage and bound to OP9 cells, but not to NK precursor cells. In addition, we confirmed that the levels of Gpnmb were increased at NK precursor stage in vivo. We confirmed syndecan4 as a candidate of Gpnmb's binding molecule. When the interaction between NK cells and OP9 cells were inhibited in vitro, IFN-${\gamma}$ production from NK cells were reduced. Conclusion: Based on these observations, it is concluded that Gpnmb has a potential role in NK cell development from HSCs.

Keywords

References

  1. Freud AG and Caligiuri MA: Human natural killer cell development. Immunol Rev 214;56-72, 2006 https://doi.org/10.1111/j.1600-065X.2006.00451.x
  2. Mrozek E, Anderson P, Caligiuri MA: Role of interleukin-15 in the development of human CD56+ natural killer cells from CD34+ hematopoietic progenitor cells. Blood 87;2632-2640, 1996
  3. Williams NS, Klem J, Puzanov IJ, Sivakumar PV, Bennett M, Kumar V: Differentiation of NK1.1+, Ly49+ NK cells from flt3+ multipotent marrow progenitor cells. J Immunol 163;2648-2656, 1999
  4. Kang HS, Kim EM, Lee S, Yoon SR, Kawamura T, Lee YC, Kim S, Myung PK, Wang SM, Choi I: Stage-dependent gene expression profiles during natural killer cell development. Genomics 86;551-565, 2005 https://doi.org/10.1016/j.ygeno.2005.06.010
  5. Rich JN, Shi Q, Hjelmeland M, Cummings TJ, Kuan CT, Bigner DD, Counter CM, Wang XF: Bone-related genes expressed in advanced malignancies induce invasion and metastasis in a genetically defined human cancer model. J Biol Chem 278;15951-15957, 2003 https://doi.org/10.1074/jbc.M211498200
  6. Selim AA, Abdelmagid SM, Kanaan RA, Smock SL, Owen TA, Popoff SN, Safadi FF: Anti-osteoactivin antibody inhibits osteoblast differentiation and function in vitro. Crit Rev Eukaryot Gene Expr 13;265-275, 2003 https://doi.org/10.1615/CritRevEukaryotGeneExpr.v13.i24.170
  7. Rose AA, Siegel PM: Osteoactivin/HGFIN: is it a tumor suppressor or mediator of metastasis in breast cancer? Breast Cancer Res 9;403, 2007 https://doi.org/10.1186/bcr1791
  8. Ogasawara K, Hida S, Azimi N, Tagaya Y, Sato T, Yokochi-Fukuda T, Waldmann TA, Taniguchi T, Taki S: Requirement for IRF-1 in the microenvironment supporting development of natural killer cells. Nature 391;700-703, 1998 https://doi.org/10.1038/35636
  9. Nakamura A, Ishii A, Ohata C, Komurasaki T: Early induction of osteoactivin expression in rat renal tubular epithelial cells after unilateral ureteral obstruction. Exp Toxicol Pathol 59;53-59, 2007 https://doi.org/10.1016/j.etp.2007.03.005
  10. Rosmaraki EE, Douagi I, Roth C, Colucci F, Cumano A, Di Santo JP: Identification of committed NK cell progenitors in adult murine bone marrow. Eur J Immunol 31;1900-1909, 2001 https://doi.org/10.1002/1521-4141(200106)31:6<1900::AID-IMMU1900>3.0.CO;2-M
  11. Roth C, Rothlin C, Riou S, Raulet DH, Lemke G: Stromalcell regulation of natural killer cell differentiation. J Mol Med 85;1047-1056, 2007 https://doi.org/10.1007/s00109-007-0195-0
  12. Chung JS, Sato K, Dougherty II, Cruz PD Jr, Ariizumi K: DC-HIL is a negative regulator of T lymphocyte activation. Blood 109;4320-4327, 2007 https://doi.org/10.1182/blood-2006-11-053769
  13. Chung JS, Dougherty I, Cruz PD Jr, Ariizumi K: Syndecan-4 mediates the coinhibitory function of DC-HIL on T cell activation. J Immunol 179;5778-5784, 2007 https://doi.org/10.4049/jimmunol.179.9.5778
  14. Vosshenrich CA, Samson-Villeger SI, Di Santo JP: Distinguishing features of developing natural killer cells. Curr Opin Immunol 17;151-158, 2005 https://doi.org/10.1016/j.coi.2005.01.005
  15. Shikano S, Bonkobara M, Zukas PK, Ariizumi K: Molecular cloning of a dendritic cell-associated transmembrane protein, DC-HIL, that promotes RGD-dependent adhesion of endothelial cells through recognition of heparan sulfate proteoglycans. J Biol Chem 276;8125-8134, 2001 https://doi.org/10.1074/jbc.M008539200
  16. Bernfield M, Gotte M, Park PW, Reizes O, Fitzgerald ML, Lincecum J, Zako M: Functions of cell surface heparan sulfate proteoglycans. Annu Rev Biochem 68;729-777, 1999 https://doi.org/10.1146/annurev.biochem.68.1.729
  17. Yamashita Y, Oritani K, Miyoshi EK, Wall R, Bernfield M and Kincade PW: Syndecan-4 is expressed by B lineage lymphocytes and can transmit a signal for formation of dendritic processes. J Immunol 162;5940-5948, 1999
  18. Kim S, Poursine-Laurent J, Truscott SM, Lybarger L, Song YJ, Yang L, French AR, Sunwoo JB, Lemieux S, Hansen TH, Yokoyama WM: Licensing of natural killer cells by host major histocompatibility complex class I molecules. Nature 436;709-713, 2005 https://doi.org/10.1038/nature03847
  19. Samson SI, Richard O, Tavian M, Ranson T, Vosshenrich CA, Colucci F, Buer J, Grosveld F, Godin I, Di Santo JP: GATA-3 promotes maturation, IFN-gamma production, and liver-specific homing of NK cells. Immunity 19;701-711, 2003 https://doi.org/10.1016/S1074-7613(03)00294-2