• Composites Research
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• 제35권3호
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• pp.153-160
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• 2022

목재 섬유 기반의 셀룰로오스 나노피브릴(cellulose nanofibrils, CNF)은 생체적합특성이 우수하여 조직 공학용 스캐폴드, 약물 운반체, 상처 치유용 겔 등의 생체 의료 분야에서 많은 관심을 받고 있다. 하지만, 셀룰로오스 나노피브릴은 상대적으로 약한 기계적 강도를 나타내기 때문에 높은 기계적 특성을 요구하는 응용 분야에 사용되기 어렵다는 한계를 가지고 있다. 따라서 본 연구에서는 셀룰로오스 나노피브릴의 기계적 강도를 향상시키기 위해 TEMPO (2,2,6,6-tetramethylpiperidin-oxyl) 산화 처리된 셀룰로오스 나노피브릴에 금속 양이온을 도입하여 금속-카르복실레이트 배위 결합을 가지는 하이드로겔을 제조하였다. 또한, 큰 진폭 진동 전단(large amplitude oscillatory shear) 측정과 Live/Dead 세포 시험을 통해 하이드로겔의 비선형 점탄성 거동과 세포 생존 능력을 분석하였다. 특히, 첨가된 금속염의 종류에 따라 세포의 증식 및 생존 능력이 변화하였고, 이는 하이드로겔들의 유변 물성 특성에도 영향을 미쳤다.

• 한국식품저장유통학회지
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• 제22권5호
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• pp.751-757
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• 2015

오디 당침출액(MSE)의 산화적 스트레스 개선 효과를 확인하기 위하여 HepG2 세포에 $H_2O_2$로 산화적 스트레스를 유도시킨 다음, MSE의 보호효과를 확인하였다. MSE를 40일간 저장하여 DPPH radical scavensing을 통해 DPPH radical 소거능이 유의적으로 좋았던 저장 40일용 MSE를 선택하여 세포 실험에 적용하였다. HepG2 세포에 $500{\mu}M$ $H_2O_2$를 처리하여 산화적 스트레스를 유발시키고, MSE를 처리하여 세포 생존율을 확인한 결과, MSE 처리로 인한 세포 생존율이 유의적으로 증가하였고, ROS 생성과 과산화물에 대한 지표로 측정된 MDA 농도도 MSE 처리로 인해 효과적으로 억제되었다. 또한, $H_2O_2$ 처리로 감소된 SOD 및 CAT 활성이 MSE 처리로 인해 유의적으로 높아졌으며, $H_2O_2$를 처리로 인한 세포핵의 apoptosis body가 MSE 처리로 인해 감소함을 확인하였으며, 이는 caspase-3 활성 MSE가 억제시킴으로 인해 세포를 보호하고 있음을 확인하였다. 이상의 결과로부터 오디 당침출액은 산화적 스트레스로부터 야기되는 세포독성과 apoptosis로부터 세포 보호 효과를 확인함에 따라 향후 노화와 관련된 다양한 연구소재의 기초 자료 및 질병 예방 소재로의 가능성을 확인하였다.

• Journal of Biosystems Engineering
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• 제38권4호
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• pp.318-326
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• 2013

Purpose: We developed a viability evaluation method for cucumber (Cucumis sativus) seed using hyperspectral reflectance imaging. Methods: Reflectance spectra of cucumber seeds in the 400 to 1000 nm range were collected from hyperspectral reflectance images obtained using blue, green, and red LED illumination. A partial least squares-discriminant analysis (PLS-DA) was developed to predict viable and non-viable seeds. Various ranges of spectra induced by four types of LEDs (Blue, Green, Red, and RGB) were investigated to develop the classification models. Results: PLS-DA models for spectra in the 600 to 700 nm range showed 98.5% discrimination accuracy for both viable and non-viable seeds. Using images based on the PLS-DA model, the discrimination accuracy for viable and non-viable seeds was 100% and 99%, respectively Conclusions: Hyperspectral reflectance images made using LED light can be used to select high quality cucumber seeds.

• 화훼연구
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• 제18권3호
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• pp.193-200
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• 2010

현재 재배되고 있는 장미 재배종은 오랜 기간 동안 종간교잡과 종내교잡을 통해 선발되어 온 것으로서 대부분이 이형잡종의 게놈으로 구성되어 있어 발아율이 저조한 편이다. 따라서, 효율적인 교배를 위해서는 화분의 활력 검정이 선행되어야 한다. 이에 본 연구에서는 염색액과 발아배지를 이용하여 7품종의 화분 임성율과 발아율 검정하고 이들 결과를 토대로 화분의 크기, 배수성 및 교배조합과의 상관관계를 알아보고자 실시하였다. 화분 임성율은 '스칼렛미미'가 82%로 가장 높았으며 '피노키오'가 39%로 가장 낮았다. 발아율 관찰 결과 '스칼렛미미'가 41%로 가장 높았으며 '미니로사'가 1%로 가장 낮았다. 화분의 크기를 관찰한 결과 화분은 큰 화분과 작은 화분으로 구분되었으며 화분의 직경은 각각 $41.3-45.4{\mu}m$과 $30.7-37.4{\mu}m$로 관찰되었으며 화분의 크기는 10-40% 정도 차이가 있는 것으로 나타났다. 화분의 크기와 염색체 전체 길이의 합을 비교한 결과 염색체 크기와 화분의 크기는 정의 상관관계가 있음을 알 수 있었다. 교배조합 검정 결과 착과율과 발아율은 화분 발아율, 임성률 및 배수성과 상관관계가 있음을 확인하였다.

• 농약과학회지
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• 제3권1호
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• pp.78-83
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• 1999

집파리(Musca domestica) 3령 유충의 먹이에 imidacloprid (30, 100 ppm)와 IGR계 살충제인 flufenoxuron (3, 10 ppm), triflumuron (10 ppm) 및 teflubenzuron (3 ppm) 등을 섞어주었을 때, 이들 약제가 번데기의 무게, 성충의 수명, 산란수, 산란된 알의 부화율 등에 미치는 영향을 실험실에서 검토하였다. Imidacloprid 100 ppm 을 처리하였을 때 번데기의 무게는 감소하였으나 산란수에는 차이가 없었으며, flufenoxuron 10 ppm을 처리하였을 때는 무처리나 다른 처리를 비해서 번데기 무게가 감소하고 산란수도 감소하였다. 그러나 처리한 약제의 종류나 농도에 관계없이 우화한 성충의 수명이나 그 성충이 낳은 알의 부화율에는 아무런 영향이 없었다.

• Food Science and Biotechnology
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• 제15권4호
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• pp.534-542
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• 2006

The effects of ethanol fractions of three different rice grain extracts, Jakwangchalbyeo, Hwasunchalbyeo, and Ilpumbyeo, on apoptotic cell death in the rat hepatoma H4IIE cell line were investigated using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] cell viability assay. One hundred mg/mL Jakwangchalbyeo extract significantly reduced cell viability to 69.5, 57.2, and 46.1% within 24, 48, and 72 hr, respectively. Fluorescence-activated cell sorting (FACS) analyses were also performed to characterize the cell death pattern caused by treatment with the rice grain extracts. Apoptotic cell death was clearly observed with time after treatment with the Jakwangchalbyeo extract. In Western blotting analysis, degradation of the 116 kDa poly-ADP-ribose polymerase (PARP) molecule was observed with concomitant formation of an 89 kDa product 24, 48, and 72 hr after treating cells with the Jakwangchalbyeo extract. This indicates that an apoptotic process caused cell death in these cells. In conclusion, red-pericarp Jakwangchalbyeo extract induced apoptotic cell death in H4IIE cells to a larger extent than the other rice extracts.

• Journal of Microbiology and Biotechnology
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• 제31권10호
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• pp.1343-1349
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• 2021

Cockroaches live in places where various pathogens exist and thus are more likely to use antimicrobial compounds to defend against pathogen intrusions. We previously performed an in silico analysis of the Periplaneta americana transcriptome and detected periplanetasin-5 using an in silico antimicrobial peptide prediction method. In this study, we investigated whether periplanetasin-5 has anticancer activity against the human leukemia cell line K562. Cell growth and survival of K562 cells treated with periplanetasin-5 were decreased in a dose-dependent manner. By using flow cytometric analysis, acridine orange/ethidium bromide (AO/EB) staining and DNA fragmentation, we found that periplanetasin-5 induced apoptotic and necrotic cell death in leukemia cells. In addition, these events were associated with increased levels of the pro-apoptotic proteins Fas and cytochrome c and reduced levels of the anti-apoptotic protein Bcl-2. Periplanetasin-5 induces the cleavage of pro-caspase-9, pro-caspase-8, pro-caspase-3, and poly (ADP-ribose) polymerase (PARP). The above data suggest that periplanetasin-5 induces apoptosis via both the intrinsic and extrinsic pathways. Moreover, caspase-related apoptosis was further confirmed by using the caspase inhibitor carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone (Z-VAD-FMK), which reversed the periplanetasin-5-induced reduction in cell viability. In conclusion, periplanetasin-5 caused apoptosis in leukemia cells, suggesting its potential utility as an anticancer therapeutic agent.

• 농업과학연구
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• 제50권4호
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• pp.785-798
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• 2023

Considering irrigation facilities are currently insufficient and drought vulnerability due to climate change is high, efficient measures are required to secure water supply for field crops. This study, therefore, calculated the water shortage to secure water for representative field crops. An economic analysis was further conducted by comparing the production income to the input cost for each method. Here, five distinct regions were selected to represent each crop-Cheongyang-gun for chili peppers, Yesan-gun for apples, Dangjin-si for cabbages, Seosan-si for garlic, and Goesan for beans. The regions with insufficient water supply were estimated by calculating the water requirements and the supplied water from public groundwater wells for each area. A comprehensive set of four scenarios was presented as a strategy to ensure water security and manage irrigation facilities. These scenarios comprised the maintenance of existing groundwater wells, the construction of new water storage tanks, the installation of additional groundwater wells, and the utilization of surface water. B/C (benefit/cost) analysis was conducted for each scenario. As a result, the construction of water storage tanks was selected as a facility and water management plan in Cheongyang-gun, Dangjin-si, and Seosan-si. The analysis additionally indicated the economic viability of installing surface water utilization facilities in Yesan-gun and developing water storage tanks and groundwater (aquifer) wells in Goesan-gun. The results of this study are considered to serve as foundation data that may be utilized in the selection of water management plans for drought-prone areas in the future.

• 생약학회지
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• 제40권3호
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• pp.173-177
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• 2009

The in vitro antibacterial activities of honeybee(Apis mellifera. L) venom collected by a bee venom collector were investigated against several bacteria including antibiotic-susceptible and resistant Propionibacterium acnes. Honeybee venom was prepared with different concentrations and they showed strong antibacterial activites. Honeybee venom inhibited the growth of the tested antibiotic-resistant P. acnes at the concentration of 1 mg/ml. The inhibitory activities of the honeybee venom showed time-dependent manner. Honeybee venom did not influence the viability of human dermal fibroblast at the high concentration of less than 10 mg/ml. From these results, we expect that honeybee venom has strong antibacterial activities and has advantage for treating cure.

• Journal of Microbiology and Biotechnology
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• 제25권8호
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• pp.1275-1280
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• 2015

Previously, we performed de novo RNA sequencing of Scolopendra subspinipes mutilans using high-throughput sequencing technology and identified several antimicrobial peptide candidates. Among them, a cationic antimicrobial peptide, scolopendrasin VII, was selected based on its physicochemical properties, such as length, charge, and isoelectric point. Here, we assessed the anticancer activities of scolopendrasin VII against U937 and Jurkat leukemia cell lines. The results showed that scolopendrasin VII decreased the viability of the leukemia cells in MTS assays. Furthermore, flow cytometric analysis and acridine orange/ethidium bromide staining revealed that scolopendrasin VII induced necrosis in the leukemia cells. Scolopendrasin VII-induced necrosis was mediated by specific interaction with phosphatidylserine, which is enriched in the membrane of cancer cells. Taken together, these data indicated that scolopendrasin VII induced necrotic cell death in leukemia cells, probably through interaction with phosphatidylserine. The results provide a useful anticancer peptide candidate and an efficient strategy for new anticancer peptide development.