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http://dx.doi.org/10.11002/kjfp.2015.22.5.751

Protective effects of mulberry (Morus alba) sugar extracts on hydrogen peroxide-induced oxidative stress in HepG2 cell  

Youn, Young (National Academy of Agricultural Science, Rural Development Administration)
Kim, Ha-Yan (National Academy of Agricultural Science, Rural Development Administration)
Park, Hoe-Man (National Academy of Agricultural Science, Rural Development Administration)
Lee, Sun-Ho (National Academy of Agricultural Science, Rural Development Administration)
Park, Jong-Ryul (National Academy of Agricultural Science, Rural Development Administration)
Hong, Seong-Gi (National Academy of Agricultural Science, Rural Development Administration)
Kim, Young-Geun (National Academy of Agricultural Science, Rural Development Administration)
Publication Information
Food Science and Preservation / v.22, no.5, 2015 , pp. 751-757 More about this Journal
Abstract
The objective of this study was to investigate the protective effects of mulberry (Morus alba) sugar extracts (MSE) against $H_2O_2$-induced oxidative stress in HepG2 cells. The MSEs was mixed with matured mulberry and sugar at the same ratio (1:1, w/w) and stored at $18{\pm}3^{\circ}C$ for 40 days. In 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging test, MSE stored for 40 days showed high activity with a ratio above 66%. Therefore, we selected 40 days as the optimum storage period. After cell viability analysis using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, we determined that the optimum concentration of MSE was 0.5%. Our results showed that MSE increased the cell viability and antioxidant enzyme activities of superoxide dismutase (SOD) and catalase in $H_2O_2$-treated HepG2 cells. Moreover, the treatment with MSE inhibited malondialdehyde (MDA) levels in $H_2O_2$-treated HepG2 cells. We also observed a reduction in apoptotic bodies in the Hoechst staining. These data show that MSE treatment significantly suppressed caspase-3 activity in HepG2 cells expored to $H_2O_2$-induced oxidative stress, thereby indicationg the protective effects of MSE in $H_2O_2$-induced oxidative stress.
Keywords
mulberry (Morus alba); sugar extracts; $H_2O_2$; oxidative stress;
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Times Cited By KSCI : 13  (Citation Analysis)
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