Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Protective effects of mulberry (Morus alba) sugar extracts on hydrogen peroxide-induced oxidative stress in HepG2 cell

오디 당침출액의 HepG2 세포에서 H2O2로 야기된 산화적 스트레스 보호 효과

  • Youn, Young (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Ha-Yan (National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Hoe-Man (National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Sun-Ho (National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Jong-Ryul (National Academy of Agricultural Science, Rural Development Administration) ;
  • Hong, Seong-Gi (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Young-Geun (National Academy of Agricultural Science, Rural Development Administration)
  • 윤영 (농촌진흥청 국립농업과학원 농업공학부) ;
  • 김하얀 (농촌진흥청 국립농업과학원 농업공학부) ;
  • 박회만 (농촌진흥청 국립농업과학원 농업공학부) ;
  • 이선호 (농촌진흥청 국립농업과학원 농업공학부) ;
  • 박종률 (농촌진흥청 국립농업과학원 농업공학부) ;
  • 홍성기 (농촌진흥청 국립농업과학원 농업공학부) ;
  • 김영근 (농촌진흥청 국립농업과학원 농업공학부)
  • Received : 2015.08.05
  • Accepted : 2015.10.14
  • Published : 2015.10.30
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Abstract

The objective of this study was to investigate the protective effects of mulberry (Morus alba) sugar extracts (MSE) against $H_2O_2$-induced oxidative stress in HepG2 cells. The MSEs was mixed with matured mulberry and sugar at the same ratio (1:1, w/w) and stored at $18{\pm}3^{\circ}C$ for 40 days. In 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging test, MSE stored for 40 days showed high activity with a ratio above 66%. Therefore, we selected 40 days as the optimum storage period. After cell viability analysis using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, we determined that the optimum concentration of MSE was 0.5%. Our results showed that MSE increased the cell viability and antioxidant enzyme activities of superoxide dismutase (SOD) and catalase in $H_2O_2$-treated HepG2 cells. Moreover, the treatment with MSE inhibited malondialdehyde (MDA) levels in $H_2O_2$-treated HepG2 cells. We also observed a reduction in apoptotic bodies in the Hoechst staining. These data show that MSE treatment significantly suppressed caspase-3 activity in HepG2 cells expored to $H_2O_2$-induced oxidative stress, thereby indicationg the protective effects of MSE in $H_2O_2$-induced oxidative stress.

오디 당침출액(MSE)의 산화적 스트레스 개선 효과를 확인하기 위하여 HepG2 세포에 $H_2O_2$로 산화적 스트레스를 유도시킨 다음, MSE의 보호효과를 확인하였다. MSE를 40일간 저장하여 DPPH radical scavensing을 통해 DPPH radical 소거능이 유의적으로 좋았던 저장 40일용 MSE를 선택하여 세포 실험에 적용하였다. HepG2 세포에 $500{\mu}M$ $H_2O_2$를 처리하여 산화적 스트레스를 유발시키고, MSE를 처리하여 세포 생존율을 확인한 결과, MSE 처리로 인한 세포 생존율이 유의적으로 증가하였고, ROS 생성과 과산화물에 대한 지표로 측정된 MDA 농도도 MSE 처리로 인해 효과적으로 억제되었다. 또한, $H_2O_2$ 처리로 감소된 SOD 및 CAT 활성이 MSE 처리로 인해 유의적으로 높아졌으며, $H_2O_2$를 처리로 인한 세포핵의 apoptosis body가 MSE 처리로 인해 감소함을 확인하였으며, 이는 caspase-3 활성 MSE가 억제시킴으로 인해 세포를 보호하고 있음을 확인하였다. 이상의 결과로부터 오디 당침출액은 산화적 스트레스로부터 야기되는 세포독성과 apoptosis로부터 세포 보호 효과를 확인함에 따라 향후 노화와 관련된 다양한 연구소재의 기초 자료 및 질병 예방 소재로의 가능성을 확인하였다.

Keywords

References

  1. Jeong IY, Lee JS, Oh H, Jung UH, Park HR, Jo SK (2003) Inhibitory effects of hot-water extracts of Paeonia japonica on oxidative stress and identification of its active components. J Korean Soc Food Sci Nutr, 32, 739-744 https://doi.org/10.3746/jkfn.2003.32.5.739
  2. Seo JE, Hwang ES, Kim GH (2011) Antioxidative and differentiation effects of Artemisia capillaris T. extract on hydrogen peroxide-oxidative damage of MC3T3-E1 osteoblast cells. J Korean Soc Food Sci Nutr, 40, 1532-1536 https://doi.org/10.3746/jkfn.2011.40.11.1532
  3. SEO JE, Kim GH (2012) Antioxidant activity and differentiation effect of Taraxacum mongolicum extracts against hydrogen peroxide-induced oxidative damage of MC3T3-E1 osteoblast cells. Korean J Food Cookery Sci, 28, 311-318 https://doi.org/10.9724/kfcs.2012.28.3.311
  4. Kim YS, Jeong DY, Shin DH (2008) Optimum fermentation conditions and fermentation characteristics of Mulberry (Morus alba) wine. Korean J Food Sci Technol, 40, 63-69
  5. Park SW, Chung IS, Ko GC (1997) Quantitative analysis of anthocyanins among mulberry cultivars and their pharmacological screening. J Korean Soc Food Sci Nutr, 38, 722-724
  6. Jung GT, Ju IO, Choi DG (2005) Quality characteristics and manufacture of mulberry wine. Korean J Food Preserv, 12, 90-94
  7. Park YS, Kang SS, Choi HJ, Yang SJ, Shon HH, Seo HH, Jeong JM (2014) Effect of mulberry (Morus alba L.) extract on blood flow improvement. J Korean Soc Food Sci Nutr, 43, 498-506 https://doi.org/10.3746/jkfn.2014.43.4.498
  8. Du Q, Zheng J, Xu Y (2008) Composition of anthocyanins in mulberry and their antioxidant activity. J Food Composition Analysis, 21, 390-395 https://doi.org/10.1016/j.jfca.2008.02.007
  9. Shin SK, Kim HJ, Kim MR (2014) Effects of mulberry concentrate on lipid oxidation of Yackwa during its storage. Korean J Food Preserve, 21, 483-490 https://doi.org/10.11002/kjfp.2014.21.4.483
  10. Kim SY, Park KJ, Lee WC (1998) Anti inflammatory and antioxidative effects of Morus spp. fruit extract. Korean J Seri Sc, 38, 100-107
  11. Kim HB, Kim SY, Ryu KS, Lee WC, Moon JY (2001) Effect of methanol extract from mulberry fruit on the lipid metabolism and liver function in cholesterol-induced hyperlipidemia rats. Korean J Seri Sci, 43, 104-108
  12. Kang CS, Ma SJ, Cho WD, Kim JM (2003) Stability of anthocyanin pigment extracted from mulberry fruit. J Korean Soc Food Sci Nutr, 32, 960-964 https://doi.org/10.3746/jkfn.2003.32.7.960
  13. Kim AJ, Yuh CS, Bang IS, Park HY, Lee GS (2007) An investigation the preparation and physicochemical properties of oddi jelly using mulberry fruit power. Korean J Food Nutr, 20, 27-33
  14. Park SY, Joo NM (2011) Processing optimization and antioxidant activity of chocolate added with mulberry. Korean J Food Sci Technol, 43, 303-314 https://doi.org/10.9721/KJFST.2011.43.3.303
  15. Yoo SS, Jeong HC (2012) Quality characteristics of pound cake with added mulberry fruit power. J East Asian Soc Dietary Life, 22, 239-245
  16. Shin SK, Kim HJ, Kim MR (2014) Quality characteristics of yackwa added with mulberry concentrate. J East Asian Soc Dietary Life, 24, 136-142
  17. Hong JH, An SH, Park GS, Choi SW, Rhee SJ (2003) Quality characteristics of mulberry fruit Selgidduk added with citric acid. Korean J Soc Food Cookery Sci, 19, 777-782
  18. Kim CR, yim SB, Kim HD, Oh HL, Jeon HL, Kim NY, Hong YP, Lee JH, Kim MR (2010) Change in the quality characteristics of salad dressing prepared with mulberry, Schidandra chinesis and yam juice during storage. Korean J Food Preserv, 17, 563-570
  19. Jung GT, Ju IO, Choi DG (2005) Quality characteristics and manufacture of mulberry wine. Korean J Food Preserv, 12, 90-94
  20. Kim KI, Kim ML (2013) Characteristics of wine fermented from mulberry juice. Korean J Culinary Res, 19, 40-51
  21. Fraga CG, Leibovitz BE, Tappel AL (1998) Lipid peroxidation measured as thiobarbituric acid-reactive substance in tissue slices: characterization and comparison with homogenates and microsome. Free Radic Biol Med, 4, 155-161
  22. Kim YR, Lee JS, Lee KR, Kim YE, Baek NI, Hong EK (2014) Effects of mulberry ethanol extracts on hydrogen peroxide-induced oxidative stress in pancreatic ${\beta}$-cells. Int J Mol Med, 33, 128-134 https://doi.org/10.3892/ijmm.2013.1534
  23. Hong CO, Hong ST, Koo YC, Yang SY, Lee JY, Lee YH, Ha YM, Lee KW (2011) Protective effect of Plantago asiatica L. extract against ferric nitrilotriacetate(Fe-NTA) induced renal oxidative stress in wistar rats. J Fd Hyg Safety, 26, 107-113
  24. Park SW, Jung YS, Ko KC (1997) Quantitative analysis of anthocyanins among mulberry cultivars and their phamacological screening. Hortic Environ Biotechnol, 38, 722-724
  25. Kim HB, Kim JB, Kim SL (2005) Varietal analysis and quantification of resveratrol in mulberry fruits. Korean J Seric Sci, 47, 51-55
  26. Bobek P (1999) Dietary tomato and grape pomace in rat: effect on lipid in serum and liver, and on antioxidant status. Br J Biomed Sci, 56, 109-113
  27. Kim HJ, Lee JM, Moon SH, Park HR (2010) Protective effect of Prunella spica extracts against $H_2O_2$-induced cytotoxicity in PC12 cells. J Life Sci, 20, 1121-1126 https://doi.org/10.5352/JLS.2010.20.7.1121

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