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http://dx.doi.org/10.4014/jmb.1503.03091

Anticancer Activity of the Antimicrobial Peptide Scolopendrasin VII Derived from the Centipede, Scolopendra subspinipes mutilans  

Lee, Joon Ha (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Kim, In-Woo (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Kim, Sang-Hee (Center for Liver Cancer, National Cancer Center)
Kim, Mi-Ae (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Yun, Eun-Young (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Nam, Sung-Hee (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Ahn, Mi-Young (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Kang, Dongchul (Ilsong Institute of Life Science, Hallym University)
Hwang, Jae Sam (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.8, 2015 , pp. 1275-1280 More about this Journal
Abstract
Previously, we performed de novo RNA sequencing of Scolopendra subspinipes mutilans using high-throughput sequencing technology and identified several antimicrobial peptide candidates. Among them, a cationic antimicrobial peptide, scolopendrasin VII, was selected based on its physicochemical properties, such as length, charge, and isoelectric point. Here, we assessed the anticancer activities of scolopendrasin VII against U937 and Jurkat leukemia cell lines. The results showed that scolopendrasin VII decreased the viability of the leukemia cells in MTS assays. Furthermore, flow cytometric analysis and acridine orange/ethidium bromide staining revealed that scolopendrasin VII induced necrosis in the leukemia cells. Scolopendrasin VII-induced necrosis was mediated by specific interaction with phosphatidylserine, which is enriched in the membrane of cancer cells. Taken together, these data indicated that scolopendrasin VII induced necrotic cell death in leukemia cells, probably through interaction with phosphatidylserine. The results provide a useful anticancer peptide candidate and an efficient strategy for new anticancer peptide development.
Keywords
Antimicrobial peptide; anticancer activity; necrosis; phosphatidylserine; scolopendrasin VII;
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