• Journal of Ginseng Research
• /
• 제35권4호
• /
• pp.504-513
• /
• 2011

In order to develop a novel system for the discrimination of five ginseng cultivars (Panax ginseng Meyer), single nucleotide polymorphism (SNP) genotyping assays with real-time polymerase chain reaction were conducted. Nucleotide substitution in gDNA library clones of P. ginseng cv. Yunpoong was targeted for the SNP genotyping assay. From these SNP sites, a set of modified SNP specific fluorescence probes (PGP74, PGP110, and PGP130) and novel primer sets have been developed to distinguish among five ginseng cultivars. The combination of the SNP type of the five cultivars, Chungpoong, Yunpoong, Gopoong, Kumpoong, and Sunpoong, was identified as 'ATA', 'GCC', 'GTA', 'GCA', and 'ACC', respectively. This study represents the first report of the identification of ginseng cultivars by fluorescence probes. An SNP genotyping assay using fluorescence probes could prove useful for the identification of ginseng cultivars and ginseng seed management systems and guarantee the purity of ginseng seed.

• 농업과학연구
• /
• 제46권1호
• /
• pp.67-78
• /
• 2019

The purpose of this study was to predict the water quality using the RNN (recurrent neutral network) and LSTM (long short-term memory). These are advanced forms of machine learning algorithms that are better suited for time series learning compared to artificial neural networks; however, they have not been investigated before for water quality prediction. Three water quality indexes, the BOD (biochemical oxygen demand), COD (chemical oxygen demand), and SS (suspended solids) are predicted by the RNN and LSTM. TensorFlow, an open source library developed by Google, was used to implement the machine learning algorithm. The Okcheon observation point in the Geum River basin in the Republic of Korea was selected as the target point for the prediction of the water quality. Ten years of daily observed meteorological (daily temperature and daily wind speed) and hydrological (water level and flow discharge) data were used as the inputs, and irregularly observed water quality (BOD, COD, and SS) data were used as the learning materials. The irregularly observed water quality data were converted into daily data with the linear interpolation method. The water quality after one day was predicted by the machine learning algorithm, and it was found that a water quality prediction is possible with high accuracy compared to existing physical modeling results in the prediction of the BOD, COD, and SS, which are very non-linear. The sequence length and iteration were changed to compare the performances of the algorithms.

• 농촌지도와개발
• /
• 제17권2호
• /
• pp.327-346
• /
• 2010

This study was carried out to investigate the status and development direction of horse riding industry for rural development of Korea. Library research and field survey were conducted to collect relevant data such as number of horse and size of horse farms, facilities for horse riding and educational institutions for youths and farmers and the results obtained were as follows : Only a few institutions among high schools, colleges and universities were observed to deliver horse riding programs. For farmers, no equestrian specialist was available in agricultural extension offices. Horse meat is consumed mostly in Jeju Island in Korea. Relevant institutions should be also strengthened including formal and nonformal education for horse riding and much more schools and universities should adopt horse riding program for the training of youths and farmers. Considering the obesity problem, the nutritional composition of horse meat is better than those of pork or beef, so promotion of horse meat consumption also should be strengthened.

• Genomics & Informatics
• /
• 제15권4호
• /
• pp.128-135
• /
• 2017

As next-generation sequencing technologies have advanced, enormous amounts of whole-genome sequence information in various species have been released. However, it is still difficult to assemble the whole genome precisely, due to inherent limitations of short-read sequencing technologies. In particular, the complexities of plants are incomparable to those of microorganisms or animals because of whole-genome duplications, repeat insertions, and Numt insertions, etc. In this study, we describe a new method for detecting misassembly sequence regions of Brassica rapa with genotyping-by-sequencing, followed by MadMapper clustering. The misassembly candidate regions were cross-checked with BAC clone paired-ends library sequences that have been mapped to the reference genome. The results were further verified with gene synteny relations between Brassica rapa and Arabidopsis thaliana. We conclude that this method will help detect misassembly regions and be applicable to incompletely assembled reference genomes from a variety of species.

• 정보관리학회지
• /
• 제35권4호
• /
• pp.77-106
• /
• 2018

본 연구는 도서관의 구체적이고 실제적인 폐교 활용방안에 앞서, 폐교 현황과 활용 사례를 기반으로 최근 도서관의 폐교 활용 트렌드를 파악하고 도서관의 폐교 활용 가능성 및 방향성을 확인하고자 하였다. 사례조사를 통하여 폐교를 활용한 도서관 건립은 복합문화공간 제공, 친환경적 공간 제공, 지역 커뮤니티 장소 제공 등 기존의 공공도서관과는 또 다른 특색을 가진 도서관으로 재탄생할 수 있음을 확인할 수 있었다. 또한, 연구 결과를 기반으로 향후 도서관의 폐교 활용 방향을 다음과 같이 제시하였다: 지역경제 기여와 지역의 재활성화 기회 제공, 지역주민을 위한 생활 밀착형 친화형 공간, 노인복지 요양서비스 귀농 생활 등과 연계한 공간의 재활용, 폐교를 활용한 부가 가치 창출과 지역 비즈니스 창출, 환경친화적 공간으로 구성, 복합문화공간의 형태로 구성된 통합적인 공간으로 활용, 지역의 커뮤니티 센터 역할 수행, 지역의 요구를 충족 보완할 수 있는 공간으로 재활용.

• Applied Biological Chemistry
• /
• 제45권4호
• /
• pp.203-206
• /
• 2002

${\gamma}-Tocopherol$ methyltransferase(TMT)는 토코페롤 생합성 대사의 마지막 단계인 감마 토코페롤을 알파 토코페롤로 변환하는데 관여하는 효소이다. 들깨의 미성숙 종자 cDNA유전자 은행에서 TMT로 추정되는 유전자를 분리하였으며 이 유전자는 1369개의 염기와 367개의 아미노산으로 구성되었으며 분자량은 약 42kDa의 추정된다. 이 cDNA는 Genbank와 상동성 분석결과 애기장대의 TMT유전자와 아미노산 수준에서 60% 정도의 상동성을 가지고 있으며 methyltransferase domain과 S-adenosyl methionine binding domain을 가지고 있으므로 TMT 유전자로 추정했다. 이 유전자의 특성을 알기 위하여 완전한 크기를 가지는 TMT유전자를 대장균에서 발현하고 invitro에서 효소의 활성을 측정하였다.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제5권1호
• /
• pp.73-77
• /
• 2002

The Sec61 trimeric complex ($\alpha$,$\beta$, and ${\gamma}$ subunits) is one of the Sec-complex responsible for post-translational protein translocation across the endoplasmic reticulum membrane in diverse organisms. In this study, a cDNA encoding the Sec61p ${\gamma}$ subunit homologue was isolated from the cDNA library of the mole cricket, Gryllotalpa orientalis. Sequence analysis of a 442-bp cDNA clone showed it to contain an open reading frame of 68 amino acid residues consisted of 204-bp. The homologues of the gene were found in the GenBank database in a diverse organism including insect, mammals, fungi, and plants. The deduced amino acid sequence of Sec61p ${\gamma}$ subunit homologue of the mole cricket showed the highest homology to the gene of the singly known insect, Drosophila melanogester (93% identity), and the least homology to that of the baker's yeast, Saccharomyces cerevisiae (37.2%). Phylogenetic analysis also confirmed a close relationship between the insect Sec61p ${\gamma}$ subunit homologues of G. orientalis and D. melanogester. Hydropathy analysis of the cricket mole and published other data suggested that the hydrophobic segment close to C-terminus is predicted to be the putative membrane anchor, Multiple alignment of the Sec61p ${\gamma}$ subunit homologue among several organisms showed the presence of several conserved domains including the conserved proline at position 28.

• Animal Bioscience
• /
• 제35권11호
• /
• pp.1698-1710
• /
• 2022

Objective: Raw potato starch (RPS) is resistant to digestion, escapes absorption, and is metabolized by intestinal microflora in the large intestine and acts as their energy source. In this study, we compared the effect of different concentrations of RPS on the intestinal bacterial community of weaned piglets. Methods: Male weaned piglets (25-days-old, 7.03±0.49 kg) were either fed a corn/soybean-based control diet (CON, n = 6) or two treatment diets supplemented with 5% RPS (RPS5, n = 4) or 10% RPS (RPS10, n = 4) for 20 days and their fecal samples were collected. The day 0 and 20 samples were analyzed using a 16S rRNA gene sequencing technology, followed by total genomic DNA extraction, library construction, and high-throughput sequencing. After statistical analysis, five phyla and 45 genera accounting for over 0.5% of the reads in any of the three groups were further analyzed. Furthermore, short-chain fatty acids (SCFAs) in the day 20 fecal samples were analyzed using gas chromatography. Results: Significant changes were not observed in the bacterial composition at the phylum level even after 20 d post feeding (dpf); however, the abundance of Intestinimonas and Barnesiella decreased in both RPS treatment groups compared to the CON group. Consumption of 5% RPS increased the abundance of Roseburia (p<0.05) and decreased the abundance of Clostridium (p<0.01) and Mediterraneibacter (p< 0.05). In contrast, consumption of 10% RPS increased the abundance of Olsenella (p<0.05) and decreased the abundance of Campylobacter (p<0.05), Kineothrix (p<0.05), Paraprevotella (p<0.05), and Vallitalea (p<0.05). Additionally, acetate (p<0.01), butyrate (p<0.05), valerate (p = 0.01), and total SCFAs (p = 0.01) were upregulated in the RPS5 treatment group Conclusion: Feeding 5% RPS altered bacterial community composition and promoted gut health in weaned piglets. Thus, resistant starch as a feed additive may prevent diarrhea in piglets during weaning.

• 한국농림기상학회지
• /
• 제22권3호
• /
• pp.176-182
• /
• 2020

작황조사시험은 우리나라주요 재배지점에 대해 해당 지역에서 주로 재배되는 품종들을 선정하여 장기적으로 벼의 생육과 수량을 관찰하는 연구이다. 이 연구의 목적은 기상 및 병해충의 변화에 따라 재배방법을 능동적으로 수정하여 대응하기 위한 것이다. 장기적인 자료 축적으로 이를 다양한 분야에서도 활용할 수 있도록 이 시험의 이루어지는 장소와 재배관리, 각 장소에 대응하는 기상관측소, 관찰되는 항목들에 대해 소개하였다. 각 관찰항목은 표준적인 절차에 따라 조사되어 일정한 품질이 유지되고 있으며 이 정보들은 현재 문헌으로 모두 공개되어 있으며 전산자료의 형식으로 획득할 수 있다.

• 한국식품저장유통학회지
• /
• 제23권7호
• /
• pp.1004-1011
• /
• 2016

본 연구에서는 국내산 대추나무의 잎과 열매의 flavonoid 배당체를 조사하기 위하여 선행 연구된 인용문헌을 바탕으로 대추나무, 묏대추나무, 야생대추나무 등 대추나무속(Zizyphus)에 따른 flavonoid의 화학적 정보 수집 및 정리하여 대추나무속의 flavonoid 라이브러리를 제작하였다. 각 flavonoid 개별성분은 UPLC-DAD-QTOF-ESI/MS를 사용하여 분석하였으며 제작된 라이브러리의 정보를 이용하여 국내산 대추나무로부터 총 6종의 flavonoid를 확인하였다. 이를 통하여 국내산 대추나무 잎의 주요 성분은 quercetin 배당체인 rutin임을 확인하였고, 특히 quercetin 3-O-robinobioside 및 isoquercitrin는 구축된 라이브러리를 바탕으로 대추나무 잎에서는 처음 확인된 것을 알 수 있었다. 본 연구와 같이 대추나무속 flavonoid의 화합물 정보를 포함하여 제작한 라이브러리는 선행된 연구와의 차이점 판단을 가능하게 할 것으로 사료된다.