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Molecular Cloning and Characterization of ${\gamma}-tocopherol$ Methyltransferase cDNA from Perilla frutescens  

Hwang, Seon-Kap (Plant Metabolic Engineering Division, National Institute of Agricultural Biotechnnology, Rural Development Administration)
Kim, Dong-Hern (Plant Metabolic Engineering Division, National Institute of Agricultural Biotechnnology, Rural Development Administration)
Lee, Jai-Youl (Department of Microbiology, Kyungpook National University)
Kim, Young-Hwan (Plant Metabolic Engineering Division, National Institute of Agricultural Biotechnnology, Rural Development Administration)
Hwang, Young-Soo (Plant Metabolic Engineering Division, National Institute of Agricultural Biotechnnology, Rural Development Administration)
Kim, Kyung-Hwan (Plant Metabolic Engineering Division, National Institute of Agricultural Biotechnnology, Rural Development Administration)
Publication Information
Applied Biological Chemistry / v.45, no.4, 2002 , pp. 203-206 More about this Journal
Abstract
${\gamma}-Tocopherol$ methyltransferase (TMT) is an enzyme catalyzing ${\gamma}-tocopherol$ into ${\alpha}-tocopherol$ at the final step of ${\alpha}-tocopherol$ synthesis pathway. Putative TMT cDNA clone specific to Perilla frutescens immature seeds was isolated from cDNA library. The cDNA clone consisted of 1369 bp open reading frame encoding 369 amino acids with a relative Mw of 42 kDa. Results revealed the CDNA has 60% homology to Arabidopsis thaliana TMT, and possesses methyltransferase and S-adenosyl methionine-binding domains, suggesting that cDNA encodes a ${\gamma}-tocopherol$ methyltransferase To characterize the properties of the TMT gene, the cDNA sequences coding for mature TMT were expressed in E. coli and assayed to determine the enzyme activity in vitro.
Keywords
Perilla frutescnes; ${\gamma}-tocopherol$ methyltransferase; cDNA;
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1 Noms, S. R., Shen, X. and DeUaPenna, D. (1998) Complementation of the Arabidopsis pdsl mutation with the gene encoding p-hydroxyphenylpyruvate dioxygenase. Plant Physiol. 117, 1317-1323   DOI   ScienceOn
2 Schultz, G., Soll, J., Fiedler, E. and Schulze-Siebert, D. (1985) Synthesis of prenylquinones in chloroplasts. Physiol. Plant 64, 123-129   DOI
3 So11, J., Kemmerling, M. and Schultz, G. (1980) Tocopherol and plastoquinone synthesis in spinach chloroplasts subtractions. Arch. Biochem. Biophys. 204, 544-550   DOI   ScienceOn
4 Shintani, D. and DellaPenna, D. (1998) Elevating the vitamin E content of plants through metabolic engineering. Science 282, 2098-2100   DOI   PUBMED   ScienceOn
5 D'harlingue, A. and Camara, B. (1985) Plastid enzymes of terpenoid biosynthesis, Purification and characterization of $\gamma$- tocopherol methyltransferase from Capsicum ctiromoplasts. J. Biol. Chem. 260, 1520-1523
6 Erm, A. N., Skrypin, V. V. and Kagan, V. E. (1985) Formation of alpha-tocopherol complexes with fatty acids Nature of complexes. Biochim Biophys. Actu 815, 209-214   DOI   ScienceOn
7 Traber, M. G. and Sies, H. (1996) Vitamin E in humans: demand and delivery. Annu. Rev. Nutr. 16, 321-437   DOI   ScienceOn
8 Shigeoka, S., Ishiko, H., Nakano, Y. and Mitsunaga, T. (1992) Isolation and properties of gamma-tocopherol methyltransferase in EugIena gracitis. Biochim Biophys. Acta 1128, 220-226   DOI
9 Sambrook, J., Fntsch, E. F. and Maniatis, T. (1989) In Mofecutar CIoning: A Laboratory Manuat Cold Spiing Harbor Laboratory, Cold Spring Harbor, NY
10 Eitenmiller, R. R. (1997) Vitamin E content of fats and oils-nutritional implications, food Technol. 51, 78-81
11 Shin, H. S. and Kim, S. W. (1994) Lipid composition of perilla seed. JAOAC 71, 619-622   DOI   ScienceOn
12 Hall, T. A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp. Ser. 41, 95-98
13 Wu, Y, Muench, D. G., Kim, Y. T, Hwang, Y. S. and Okita, T. W. (1998) Identification of pplypeptides associated with an enriched cytoskeleton-protein body fraction from developing lice endosperm. Ptant Cell Physiol. 39, 1251-125   DOI
14 Pawlowski, K., Kunze, R., De bries, S. and Bisseling, T. (1994) In Plant Molecular Biotogy ManuaI (2nd ed.): Isolation of total, Poly (A) and polysomat RNA from plant tissues Kluwer Academic Publishers, Belgium. D5 pp. 1-13