• The Plant Pathology Journal
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• 제33권4호
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• pp.429-433
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• 2017

Chrysanthemums (Chrysanthemum morifolium) are susceptible to tobacco mosaic virus (TMV). TMV-based expression vectors have been used in high-throughput experiments for production of foreign protein in plants and also expressing green fluorescent protein (GFP) to allow visualization of TMV movement. Here, we used TMV expressing the GFP to examine the infection of chrysanthemum by a TMV-based expression vector. Viral replication, movement and GFP expression by TMV-GFP were verified in upper leaves of chrysanthemums up to 73 days post inoculation (dpi) by RT-PCR. Neither wild-type TMV nor TMV-GFP induced symptoms. GFP fluorescence was seen in the larger veins of the inoculated leaf, in the stem above the inoculation site and in petioles of upper leaves, although there was no consistent detection of GFP fluorescence in the lamina of upper leaves under UV. Thus, a TMV-based expression vector can infect chrysanthemum and can be used for the in vivo study of gene functions.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2012년도 학술발표회
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• pp.707-707
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• 2012

현재 농업용저수지 둑 높이기 사업이 전국 110개 저수지를 대상으로 추진되고 있다. 다양한 효과가 기대되고 있는 둑 높이기 사업과 더불어 농업생산기반시설 및 주변지역 활용에 관한 특별법의 시행으로 저수지 등 농업기반시설 및 그 주변지역을 본래의 기능을 유지하는 범위에서 개발할 수 있게 됨에 따라 다양한 시설물의 설치와 운영을 통해 지역의 수익창출이 가능하게 되었다. 그러나 저수지 수변지역으로 개발계획이 집중되어 있고 추가확보 되는 용수는 하천유지와 농업용수 공급에 주로 활용될 계획이므로 추가확보 용수의 보다 다양한 활용방안을 제고하여 더 많은 이익을 창출할 수 있는 방안이 요구되고 있다. 그 중 하나로 둑 하류지역 농경지의 관개배수체계를 정비하여 추가용수 이용의 효율을 향상시키고 농촌마을과 연계하여 친수공간을 조성함으로서 둑하류 농촌마을 주민의 삶의 질 향상과 경제적 이득을 창출할 수 있는 기회를 제공할 수 있을 것이다. 이와 같은 친수공간 개발은 둑높이기 저수지의 추가용수 확보규모 및 지역의 지형, 토지이용, 사회경제적 특성에 따라 달라질 수 있으나 유형화를 통해 대표적 특성에 대한 표준안을 개발 할 수 있을 것이다. 따라서 본 연구에서는 기초연구로서 둑 높이기 저수지의 특성 및 주변 현황자료를 수집하고 통계적인 기법을 이용하여 5개의 둑하류 농촌지역 친수공간 개발유형을 분류하였으며 지역 및 공간적 분포를 제시하였다.

• Osong Public Health and Research Perspectives
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• 제8권5호
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• pp.308-317
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• 2017

Objectives: We aimed to examine the effects of various leisure activities on cognitive impairment in young-old (aged 65-74 years) and old-old (aged ${\geq}75$ years) adults. Methods: In total, 10,279 elderly Korean individuals from the 2014 Korean National Survey on Older Adults' cohort were enrolled in our study. Cognitive impairment was assessed using the standardized score of the Mini-Mental State Examination for Dementia Screening, whereas leisure activities were recorded via self-reporting of the extent and type of leisure activity the subjects involved in over the past year. Multivariate logistic regression was used to assess the effect of leisure activities on cognitive impairment, while controlling for potential covariates. Results: The subjects were more likely to participate in cognitive activities than in non-exercise physical activities. After controlling for selected covariates, involvement in cognitive activities was found to be a significant predictor of cognitive impairment in both the groups, whereas involvement in non-exercise physical activities was not a predictor of cognitive impairment in individuals aged ${\geq}75$ years. Moreover, depressive symptoms, rural residence, and hearing difficulties were common predictors of cognitive impairment among elderly-Korean-individuals. Conclusion: Leisure activity involvement may help delay cognitive impairment, which is often concomitant with aging. Hence, an early intervention service may significantly benefit both young-old and old-old individuals.

• Plant Resources
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• 제7권1호
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• pp.39-46
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• 2004

To demonstrate the importance of transformation efficiency in independent event, molecular and cytogenetic analysis were conducted with genomic DNA and chromosome of transgenic plants produced by Agrobacterium tumefeciens LBA4404 (pSBM-PPGN: gusA and bar). Selection ratios of putative transgenic calli were similar in independent experiments, however, transformation efficiencies were critically influenced by the type of regeneration media. MSRK5SS-Pr regeneration mediun, which contains 5 mgL$^{-1}$ kinetin, 2% (w/v) sucrose in combination with 3% (w/v) sorbitol, and 500 mgL$^{-1}$ proline, was efficient to produce transgenic plant of rice from putative transgenic callus in the presence of L-phosphinotricin (PPT). With MSRK5SS-Pr medium, transformation efficincies of Nagdongbyeo were significantly enhanced from 3.7% to 6.3% in independent callus lines arid from 7.3% to 19.7% in plants produced, respectively. Stable integration and expression of bar gene were confirmed by basta herbicide assay, PCR amplification and Southern blotting of bar gene, and fluorescence in situ hybridization (FISH) analysis using pSBM-PPGN as a probe. In Southern blot analysis, diverse band patterns were observed in total 44 transgenic plants regenerated from 20 independent PPT resistant calli showing from one to five copies of T-DNA segments, however, the transformants obtained from one callus line showed the same copy numbers with the same fractionized band patterns.

• 한국육종학회지
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• 제40권4호
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• pp.495-498
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• 2008

A new spray chrysanthemum cultivar, 'Golden Festival' was released by National Horticultural Research Institute (NHRI), Rural Development Administration (RDA), Korea in 2007. It was selected from the progenies of open-pollination of 'Torbay' in 2002. Trials were conducted from 2005 to 2007 for evaluation and selection of this cultivar, including shading culture in summer and retarding culture in autumn. The natural flowering time of 'Golden Festival' is late October, but year-round flowering is possible by shading and lighting treatment. The cultivar is semi-double type flowers with bright yellow petals and green flower center. Flower neck and stem are very hardy. The diameter of flower is 56.5 mm. The number of flowers per stem and petals per flower are 12.0 and 93.5, respectively. The days to flowering under the short day treatment is about 50 in spring season.

• 농업과학연구
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• 제46권3호
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• pp.683-687
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• 2019

The leafhopper genus Batracomorphus Lewis, 1834 belongs to the tribe Batracomorphini (Hemiptera: Auchenorrhyncha: Cicadellidae: Iassinae). B. irroratus Lewis, 1834 was designated as its type species. The genus includes most diverse species in the subfamily Iassinae and contains approximately 350 described species from the world. In Korea, four Batracomorphus species, B. allioni (Turton, 1802), B. punctilliger Anufriev, 1981, B. stigmaticus (Matsumura, 1912), B. viridulus (Melichar, 1902), have been reported in the Korean peninsula. The members of this genus have a similar body size and similar general appearances; thus, it is very difficult to identify clearly without examining the male's genital structures. In this study, Batracomorphus furcatus Li and Wang, 2003 is newly reported from Korea for the first time. This species is previously known to be from China. Herein, the morphological information of B. furcatus Li and Wang is provided with a re-description, photographs of the male dorsal habitus, illustrations of the genitalia structure and distributional records. In addition, a key to the Korean five species of Batracomorphus is presented.

• The Plant Pathology Journal
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• 제35권5호
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• pp.486-497
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• 2019

Citrus canker is a devastating disease of citrus caused by Xanthomonas citri subsp. citri (Xcc). A total of 134 endophytic bacteria were isolated from various gymnospermic and angiospermic plants. They were screened for their antagonistic activities against three wild-type and six streptomycin-resistant Xcc strains. TbL-22 and TbL-26, both later identified as Bacillus thuringiensis, inhibited all the wild and resistant Xcc strains. TbL-22 exerted the highest antagonistic activity against XccW3 and XccM6 with inhibition zones of $20.64{\pm}0.69$ and $19.91{\pm}0.87mm$, respectively. Similarly ethyl acetate extract of TbL-22 showed highest inhibition zones $15.31{\pm}2.08$ and $19.37{\pm}3.17mm$ against XccW3 and XccM6, respectively. TbL-22 reduced canker incidence on infected leaves by 64.05% relative to positive controls. Scanning electron microscopy revealed that the cell membranes of Xcc treated with ethyl acetate extract of TbL-22 were ruptured, lysed, and swollen. B. thuringiensis TbL-22 can effectively and sustainably controls streptomycin-resistant citrus canker.

• International Journal of Computer Science & Network Security
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• 제21권8호
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• pp.55-64
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• 2021

In the last few years, the massive development in wireless networks, high internet speeds and improvement in car manufacturing has shifted research focus to Vehicular Ad-HOC Networks (VANETs). Consequently, many related frameworks are explored, and it is found that security is the primary issue for VANETs. Despite that, a small number of research studies have taken into consideration the identification of performance standards and parameters. In this paper, VANET security frameworks are explored, studied and analysed which resulted in the identification of a list of performance evaluation parameters. These parameters are defined and categorized based on the nature of parameter (security or general context). These parameters are identified to be used by future researchers to evaluate their proposed VANET security frameworks. The implementation paradigms of security frameworks are also identified, which revealed that almost all research studies used simulation for implementation and testing. The simulators used in the simulation processes are also analysed. The results of this study showed that most of the surveyed studies used NS-2 simulator with a percentage of 54.4%. The type of scenario (urban, highway, rural) is also evaluated and it is found that 50% studies used highway urban scenario in simulation.

• 건축역사연구
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• 제30권3호
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• pp.7-20
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• 2021

Since 1939, Bupeyong was rapidly urbanized and industrialized for the purpose of constructing military logistics base of the empire of Japan. Based on Kyungin urban planning of 1940 and industrial land development plan of Kyeonggido, many military factories and arsenal were constructed, and great amount of housing were also built for their workers from 1939 to 1944. Although the initial urban planning was unfinished, urban change from rural area to military industrial city in the late Japanese colonial era, made identity of city of Bupyeong. Workers houses which built during five years vary in different size and type, and somewhat reflects discussions about housing attempts to solve the housing shortage. This study aims to analyze architectural characteristics of workers housing related with discussions of housing, and to evaluate its value as an industrial heritage which constitute the urban landscape of the industrial city of Bupyeong. Workers houses in Bupyeong were constructed by military factories, construction company, and Chosun housing administration, with land readjustment planning. As the war became serious, workers housing became smaller and simpler. Construction of workers housing was essential part of modern military industrial city, bupyeong, and many of workers housing are still remained whereas most of factories were demolished, thus workers housing of bupyeong has significant meaning as industrial heritage of Bupyeong.

• International Journal of Knowledge Content Development & Technology
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• 제10권4호
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• pp.47-64
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• 2020

The study investigated the role of libraries in curtailing the spread of COVID-19 pandemic in Nigeria. Six research objectives were raised to guide the conduct of the study. The survey type of the descriptive research design was employed. The population of the study comprised of 139 selected librarians in Nigeria. The complete enumerative sampling technique was adopted to study the entire 139 librarians. The instrument for data collection was an online survey questionnaire designed by the researchers using SurveyMonkey and administered to the respondents via the Nigerian Library Association (NLA) WhatsApp group and the NLA Google group. The data collected were analyzed using descriptive statistics. The study revealed that among the services delivered by Nigerian libraries amidst the global pandemic are COVID-19 awareness and sensitization creation, provision of digital contents to patron, organizing online streaming programs and connecting patrons/citizens to accurate and reliable data on COVID-19 cases around the world. The study further revealed that these services are delivered frequently despite the low level of Nigerian libraries' preparedness for global pandemic. The study concluded that, Nigerian libraries, through their services, are potent weapon for promoting positive change, development, orientation, and re-orientation in the society. By creating COVID-19 awareness/sensitization (especially in rural areas), libraries are able to contribute significantly to the fight against the pandemic.