[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5423/PPJ.OA.03.2019.0060

Biocontrol of Citrus Canker Disease Caused by Xanthomonas citri subsp. citri Using an Endophytic Bacillus thuringiensis

Islam, Md. Nurul (Department of Biotechnology, Yeungnam University)
Ali, Md. Sarafat (Department of Biotechnology, Yeungnam University)
Choi, Seong-Jin (Department of Biotechnology, Catholic University of Daegu)
Hyun, Jae-Wook (Citrus Research Station, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Baek, Kwang-Hyun (Department of Biotechnology, Yeungnam University)

Publication Information

The Plant Pathology Journal / v.35, no.5, 2019 , pp. 486-497 More about this Journal

Abstract

Citrus canker is a devastating disease of citrus caused by Xanthomonas citri subsp. citri (Xcc). A total of 134 endophytic bacteria were isolated from various gymnospermic and angiospermic plants. They were screened for their antagonistic activities against three wild-type and six streptomycin-resistant Xcc strains. TbL-22 and TbL-26, both later identified as Bacillus thuringiensis, inhibited all the wild and resistant Xcc strains. TbL-22 exerted the highest antagonistic activity against XccW3 and XccM6 with inhibition zones of $20.64{\pm}0.69$ and $19.91{\pm}0.87mm$ , respectively. Similarly ethyl acetate extract of TbL-22 showed highest inhibition zones $15.31{\pm}2.08$ and $19.37{\pm}3.17mm$ against XccW3 and XccM6, respectively. TbL-22 reduced canker incidence on infected leaves by 64.05% relative to positive controls. Scanning electron microscopy revealed that the cell membranes of Xcc treated with ethyl acetate extract of TbL-22 were ruptured, lysed, and swollen. B. thuringiensis TbL-22 can effectively and sustainably controls streptomycin-resistant citrus canker.

Keywords

Bacillus thuringiensis; biocontrol; citrus canker; endophytic bacteria;

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Times Cited By KSCI : 3 (Citation Analysis)

Reference
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