Plant Resources
- Volume 7 Issue 1
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- Pages.39-46
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- 2004
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- 1226-8771(pISSN)
Molecular and Cytogenetic Analysis of Transgenic Plants of Rice(Oryza sativa L.) Produced by Agrobacterium-mediated Transformation
- Cho, Joon-Hyeong (Division of Environment and Biotechnology, National Institute of Crop Science, Rural Development Adminstration) ;
- Kim, Yong-Wook (Department of Plant Resources, Dongguk University)
- Published : 2004.04.01
Abstract
To demonstrate the importance of transformation efficiency in independent event, molecular and cytogenetic analysis were conducted with genomic DNA and chromosome of transgenic plants produced by Agrobacterium tumefeciens LBA4404 (pSBM-PPGN: gusA and bar). Selection ratios of putative transgenic calli were similar in independent experiments, however, transformation efficiencies were critically influenced by the type of regeneration media. MSRK5SS-Pr regeneration mediun, which contains 5 mgL
Keywords
- Agrobacterium-mediated transformation;
- Embryogenic callus;
- Rice;
- Transformation efficiency;
- FISH;
- Southern blotting analysis