The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Biocontrol of Citrus Canker Disease Caused by Xanthomonas citri subsp. citri Using an Endophytic Bacillus thuringiensis

  • Islam, Md. Nurul (Department of Biotechnology, Yeungnam University) ;
  • Ali, Md. Sarafat (Department of Biotechnology, Yeungnam University) ;
  • Choi, Seong-Jin (Department of Biotechnology, Catholic University of Daegu) ;
  • Hyun, Jae-Wook (Citrus Research Station, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Baek, Kwang-Hyun (Department of Biotechnology, Yeungnam University)
  • Received : 2019.03.20
  • Accepted : 2019.08.12
  • Published : 2019.10.01
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Abstract

Citrus canker is a devastating disease of citrus caused by Xanthomonas citri subsp. citri (Xcc). A total of 134 endophytic bacteria were isolated from various gymnospermic and angiospermic plants. They were screened for their antagonistic activities against three wild-type and six streptomycin-resistant Xcc strains. TbL-22 and TbL-26, both later identified as Bacillus thuringiensis, inhibited all the wild and resistant Xcc strains. TbL-22 exerted the highest antagonistic activity against XccW3 and XccM6 with inhibition zones of $20.64{\pm}0.69$ and $19.91{\pm}0.87mm$, respectively. Similarly ethyl acetate extract of TbL-22 showed highest inhibition zones $15.31{\pm}2.08$ and $19.37{\pm}3.17mm$ against XccW3 and XccM6, respectively. TbL-22 reduced canker incidence on infected leaves by 64.05% relative to positive controls. Scanning electron microscopy revealed that the cell membranes of Xcc treated with ethyl acetate extract of TbL-22 were ruptured, lysed, and swollen. B. thuringiensis TbL-22 can effectively and sustainably controls streptomycin-resistant citrus canker.

Keywords

References

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