• The Plant Pathology Journal
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• v.33 no.5
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• pp.458-466
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• 2017

Xanthomonas oryzae pv. oryzae (Xoo), the causative agent of bacterial blight, is a major threat to rice productivity. Here, we performed RNA-Seq based transcriptomic analysis of Xoo transcripts isolated under in planta growth (on both susceptible and resistant hosts) and in vitro culture conditions. Our in planta extraction method resulted in successful enrichment of Xoo cells and provided RNA samples of high quality. A total of 4,619 differentially expressed genes were identified between in planta and in vitro growth conditions. The majority of the differentially expressed genes identified under in planta growth conditions were related to the nutrient transport, protease activity, stress tolerance, and pathogenicity. Among them, over 1,300 differentially expressed genes were determined to be secretory, including 184 putative type III effectors that may be involved in Xoo pathogenicity. Expression pattern of some of these identified genes were further validated by semi-quantitative RT-PCR. Taken together, these results provide a transcriptome overview of Xoo under in planta and in vitro growth conditions with a focus on its pathogenic processes, deepening our understanding of the behavior and pathogenicity of Xoo.

• Journal of Preventive Medicine and Public Health
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• v.33 no.3
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• pp.271-279
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• 2000

Objectives : To assess the seroprevalence and risk factors of toxoplasmosis in high school students in Cheju Province, Korea. Methods : A total of 4,570 high school students from 18 schools in Cheju Province were investigated for Toxoplasma gondii antibodies(IgG) by enzyme linked immunosorbent assay (ELISA). Risk factors for toxoplasmosis, such as place of residence, type of house, contact with cats and other pets, and rare meat consumption, were examined by questionnaire. Results . The overall antibody positive rate was 5.5% and ranged from 2.6 to 11.5% by school. There was no significant difference between males and females. Statistical analyses of the questionnaire data indicated that the risk factors for seropositivity were: (1) birth place (Cheju/others), (2) place of residence (rural/urban), (3) dietary habits (vegetarian/non vegetarian), (4) eating rare meat, (5) exposure to pets and (6) hepatitis B. Conclusion : We confirmed that the prevalence of the anti-Toxoplasma gondii antibody in a population of high school students in Cheju Province was to the previously reported prevalence.

• Journal of Veterinary Clinics
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• v.8 no.2
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• pp.153-156
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• 1991

The study on leptospiral infection among cows was carried out to find out possible role of this domestic animal as an infection source for humans, and the magnitude of economic loss due to abortions caused by leptospiral infections among the domestic animal. Bood samples of 459 cows in May and 446 heads in November 1989 were collected from 19 villages of Kyunggi Province. These samples were cultured and serologically tested. Only one strain of leptospires, most strongly reactive to Pomona antibody, was isolated from duplicated cultures of 985 cow blood samples in EMJH medium. Serological test by MAT with seven reference strains and three locally isolated strains was positive in 12% of the sample in May and 26% in November ; positive rate increased slightly as the age of cows increased and male cows had higher positive rate, however, there was on difference in positive rate by type of cow and area. There was no statistically significant association between abortion history among cows(one yearperiod) and positive MAT. Thus it was concluded that the leptospiral infection of cows may play a role for human infection, and the leptospiral infection of cow may not be the major cause of abortion although further study is necessary for definite conclusion.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.9
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• pp.3599-3609
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• 2010

Recently, the construction of coastal structures and high-rise structures against the horizontal and uplift forces increases with the developing the coastal developments. Especially the application of belled tension pile as foundation type to effectively resist uplift force is increasing in coastal structures. However, research on pullout resistance of belled tension pile has been limited and not yet been fully performed. Therefore, the pullout load tests of belled tension piles in four overseas sites were performed, then the bearing capacity, characteristics on load-displacement of piles and load distribution considering skin friction were investigated in this paper. In addition, the limit pullout bearing capacity calculated by the three-dimensional finite element analysis and theoretical methods were compared with values of in-situ test.

• Korean journal of food and cookery science
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• v.11 no.4
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• pp.370-378
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• 1995

This study is to seek methods for optimum management of foodservice system for elementary schools in Korea through surveying and analyzing the current status of the "common-management foodservice system" and certain important factors relating to its operations. For the survey of the current status of the "common-management foodservice system", questionnaires are prepared as against schools adopting "common-management system"("Common Schools") as well as schools adopting "conventional management system" ("Conventional Schools") in Kyungsangbookdo ("Kyungbook") area. The survey items are composed of general items, items concerning tasks of dietitians, items concerning purchasing activities and items concerning level of recognition and job-satisfaction of the dietitians. Total 46 dietitians from Common Schools and 65 dietitians from Conventional Schools in Kyungbook region responded the questionnaires. The results of this study are as follows: 1. Information Concerning Dietitians Average age of the dietitians: 26.6 yrs Ratio of two years or less of experiences as school dietitian: Dietitians from Common Schools: 59.1％ Dietitians from Conventional Schools: 50.8％ 2. Types of the Foodservice In both schools, rural type are prevalent; however, between urban types and islands types, the ratio of urban types are larger than the ratio of islands types in Conventional Schools, and the opposite is true in Common Schools. 3. Decision of Suppliers The ratio of dietitian＇s participaion in the decision making reaches only 38.3％, indicating the possibility of problems in quality control and hygienic aspects of the food supply. 4. Factor Analysis of the Foodservice Duties Food supply and general affairs administration work was carried out very well (4.2), while personnel management and operational improvement work (2.8), nutritional education work (2.3), and information related work were poorly carried out. No significant differences were found between the two types of the foodservice management systems. 5. Job Satisfaction scores of Dietitians Dietitians were more satisfied with the works (50.0), supervision (50.5) and co-workers (46.9) than pay (18.0) and promotion (22.3).

• Journal of the Korean Society of Environmental Restoration Technology
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• v.9 no.2
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• pp.1-15
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• 2006

As the imports of foreign agricultural products are liberalized and the consumption of agricultural products declines, abandoned rice paddy fields continues to rise. However, such abandoned rice paddy fields has not been precisely surveyed yet. In this backdrop, a necessity to develop technology to utilize such abandoned rice paddy fields has emerged. Utilization of abandoned rice paddy fields as wetlands may be a good example. This study aimed to survey the current status and characteristics of wetlands created within abandoned rice paddy fields by selecting abandoned rice paddy fields throughout the nation and conducting field surveys on the sites that had transformed into wetlands. The abandoned rice paddy fields almost transformed into wetland and the types of wetlands transformed from abandoned rice paddy fields were mainly Inland/Moutain/Depression/Abandoned rice paddy fields/Marsh/Phragmites communis community and Inland/Moutain/Depression/Abandoned rice paddy fields/Swamp/Salix koreensis community. Abandoned rice paddy fields that had transformed into wetlands was depending heavily on waterways for water supply than other reservoirs and lakes do. Abandoned rice paddy fields transformed into wetlands was most observed in mountainous area. Abandoned rice paddy fields are because agricultural land is no longer profitable due to international and social changes and is not cultivated as government policy. Wetland period and dimension originated from abandoned rice paddy fields are very various and its surrounding land its mostly forest and the next largest follow roads and rural community. The abandoned rice paddy fields transformed into wetlands is mostly deserted currently. Despite their value as wetlands, no restoration and utilization efforts are made in Korea today. Therefore, it is imperative to conduct a precise current status survey on these areas and introduce management and restoration plans at the government level in the case of important habitats.

• Journal of Biosystems Engineering
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• v.35 no.4
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• pp.215-223
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• 2010

In order to develop an precision aerial pesticide application system to be attached to an unmanned helicopter which can be applied to small lots of land, this study analyzed the flowing and spraying characteristics of the spray droplets by the main rotor downwash by setting the application conditions at the flight altitude of 3 m, the diameter of main rotor of 3.1 m, the boom length of around 2.8 m, and the spraying rate of 8 L/ha. The results of this study are summarized below. Through analysis of the covering area ratio of the spray droplets by main rotor downwash by nozzle type, boom with tilt angle and height, it was found that the covering area ratio of the twin flat-fan nozzle of around 25% was more uniform than other types of nozzle, also boom with $10^{\circ}$ tilt angle and spraying height of 3 m was shown to be the appropriate conditions for aerial application of pesticides. It was found that the nozzle position to minimize the scattering loss of spray droplets due to vortex phenomenon at both ends of the main rotor was around 10 cm from the end of the main rotor. An application test for the aerial pesticide application system attached to the HUA-ACEI unmanned helicopter developed by the Rural Development Administration showed that the range of covering area ratio of the spray droplets was 10-25%, and the spraying width was approximately 7 m when over 10% of covering area ratio was considered for valid spraying.

• Health Policy and Management
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• v.20 no.1
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• pp.125-136
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• 2010

Objectives: The purpose of this study is to analyze the proportion of fundus examination of DM patients for preventing microvascular complication and to examine the factors related to fundus examination in DM patients. Methods: This study used 2005 Korean national health and nutrition survey data. We selected all 1,129 diabetes mellitus patients from the data. And we choose 8 factors related to diabetes mellitus patients. These are sex, age group, type of residential area(rural or urban), education level, income level, comorbidity with hypertension, current insulin use, and duration of suffering from DM. We have analysed these factors by whether he had been examined fundus or no through Chi-square and logistic regression analysis. Results: Female DM patients have tendency to get more fundus examination than Male DM patients. And Young patients, patients who live in urban area, well educated patients, high income patients, comorbidity with hypertension, patients have got the insulin injection, patients have long duration of suffering from DM are to get more fundus examination. According to multivariate logistic regression analysis, sex and education level, income level, and duration of suffering from DM, comorbidity with hypertension, patients have got the insulin injection are significant factors on fundus examination for prevent microvascular diabetes mellitus complications. Conclusions and Discussion: We have concluded that physicians and policy makers should consider to fundus examination especially of man and DM patients who have more shorter disease period and low educated patients and low incomed patients and patients who have taken insulin therapy.

• Journal of the Korean Society for information Management
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• v.18 no.1
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• pp.23-42
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• 2001

The purpose of this study was to obtain descriptive information about public library connectivity and use related to the Internet in Korea. Questionnaire was sent to all of the 364 public libraries in the nation. The data show that, as of the end of 1999,70.9% of the 351 public libraries which answered the questionnaire had some type of connection to the Internet, and 50.4% of them provided public Internet access services. The infrastructure of hardware and telecommunication, use policy for their public access Internet services were analysed. Public librarians were asked to evaluate their public Internet services. Overall the study found that the level of the nation' s public library Internet services were not satisfactory in quality and quantity. It also found that there were some significant disparities among public libraries in metropolitan areas, medium and small sized cities, and rural counties.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.2
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• pp.157-162
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• 2018

Objective: The aim of this study is to identify single nucleotide polymorphisms (SNPs) and genes related to pig IMF and estimate the heritability of intramuscular fat content (IMF). Methods: Genome-wide association study (GWAS) on 704 inbred Berkshires was performed for IMF. To consider the inbreeding among samples, associations of the SNPs with IMF were tested as random effects in a mixed linear model using the genetic relationship matrix by GEMMA. Significant genes were compared with reported pig IMF quantitative trait loci (QTL) regions and functional classification of the identified genes were also performed. Heritability of IMF was estimated by GCTA tool. Results: Total 365 SNPs were found to be significant from a cutoff of p-value <0.01 and the 365 significant SNPs were annotated across 120 genes. Twenty five genes were on pig IMF QTL regions. Bone morphogenetic protein-binding endothelial cell precursor-derived regulator, forkhead box protein O1, ectodysplasin A receptor, ring finger protein 149, cluster of differentiation, tyrosine-protein phosphatase non-receptor type 1, SRY (sex determining region Y)-box 9 (SOX9), MYC proto-oncogene, and macrophage migration inhibitory factor were related to mitogen-activated protein kinase pathway, which regulates the differentiation to adipocytes. These genes and the genes mapped on QTLs could be the candidate genes affecting IMF. Heritability of IMF was estimated as 0.52, which was relatively high, suggesting that a considerable portion of the total variance of IMF is explained by the SNP information. Conclusion: Our results can contribute to breeding pigs with better IMF and therefore, producing pork with better sensory qualities.