• Molecules and Cells
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• v.38 no.9
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• pp.773-780
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• 2015

3D8 single chain variable fragment (scFv) is a recombinant monoclonal antibody with nuclease activity that was originally isolated from autoimmune-prone MRL mice. In a previous study, we analyzed the nuclease activity of 3D8 scFv and determined that a HeLa cell line expressing 3D8 scFv conferred resistance to herpes simplex virus type 1 (HSV-1) and pseudorabies virus (PRV). In this study, we demonstrate that 3D8 scFv could be delivered to target tissues and cells where it exerted a therapeutic effect against PRV. PRV was inoculated via intramuscular injection, and 3D8 scFv was injected intraperitoneally. The observed therapeutic effect of 3D8 scFv against PRV was also supported by results from quantitative reverse transcription polymerase chain reaction, southern hybridization, and immunohistochemical assays. Intraperitoneal injection of 5 and $10{\mu}g$ 3D8 scFv resulted in no detectable toxicity. The survival rate in C57BL/6 mice was 9% after intramuscular injection of 10 $LD_{50}$ PRV. In contrast, the 3D8 scFv-injected C57BL/6 mice showed survival rates of 57% ($5{\mu}g$) and 47% ($10{\mu}g$). The results indicate that 3D8 scFv could be utilized as an effective antiviral agent in several animal models.

• Journal of the Korean Dietetic Association
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• v.3 no.2
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• pp.169-181
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• 1997

In this study, I made researches of 510 mothers who breed their infants in total (256 boys & 254 girls), among which 410 in urban area and 100 in the rural area, as an object of the investigation so as to make an intensive examination and analysis of the actual state of feeding nutritive method, grasp several related factors, and finally suggest the elementary data for both campaign encouraging breast feeding and the infant feeding education. 1. Mothers showed the meaningful local differences as in an education and income level at 1% and as in a delivery type at 5%. 2. To have decided the feeding conditions according to the residences by the Kaup index distribution (P<0.05) resulted in a meaningful difference according to regions and also made us be more thoughtful of the overnutrition in the urban area than in the rural area, and it didn't showed a meaningful difference the nutritive state according to the infant feeding method thought desirable (P<0.01). 3. The feeding methods consist of breast feeding at 21.8%, artificial feeding at 48.8%, and the mixed one at 29.4%. As for the feeding method thought desirable, breast feeding was 52%. Even though they were thinking that breast feeding is better, the breast feeding case didn't go beyond 32.5% only and as for the actual feeding methods it showed a meaningful difference according to the feeding method thought desirable (P<0.01). 4. Regarding the reasons why they chose breast milk, it was 64%, the most because mother's milk contains the immune system, 63.1% for the baby's health, 52.3% because of its most because breast milk is of little quantity, 37.8% because of the mother's occupation, 14.1% because they have fed the baby artificially from the beginning and so on. Considering the reasons why they chose the mixed feeding, it was 70.7%, the most because the amount of breast milk a little, 27.3%, the next one, because of the mother's occupation and so on. 5. The respondents who answered that how to increase the number of breast feeding population depends on the reinforcement of breast feeding education for the women were 190 (37.3%), the most among all 150 persons and those who had the opinion that they should spread and actualize the temporary childcare rest (from office) system for the women who have jobs were the secondly most, 107 (21%).

• The Journal of the Korea Contents Association
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• v.20 no.4
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• pp.358-372
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• 2020

Recently, there has been growing social interest in the use of care farms as part of therapy for neurological or mental patients and regional innovation for rural areas. Care farm, which combines the health treatment and rural innovation perspectives, is important to establish a proper governance system for mutual collaboration with various stakeholders. However, there is still a lack of research on this. Therefore, this study designed the care farm governance framework and comparatively analyzed the governance between Netherlands paradise care farm and Belgium blue farm, which are major successful care farm cases. The results showed different governance system between Netherlands and Belgium care farm in terms of institutional and financial support, regional characteristics, structure, operation, and strategy. In Netherlands, as the central government-initiated therapy-centric, care farm governance is concentrated in treatment of patient mainly supported by Ministry of Health, Welfare and Sports under the law on social support. Whereas, in Belgium, as local government-initiated agriculture-centric, care farm governance is mainly focused to lead voluntary participation of agricultural cooperatives and medical institutions in regional area. This study provides a theoretical foundation of governance type and system for the care farm research and suggests guidelines of care farm governance for the governments like South Korea consider activating care farm.

• Environmental and Resource Economics Review
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• v.26 no.2
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• pp.229-255
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• 2017

This study empirically estimates the impacts of climate change on forest composition in Korea using a fractional data regression model, and forecasts the change in forest composition in the 2040s and 2090s based on the IPCC climate change scenarios. Unlike the forest science studies that incorporate mostly only ecological variables as the determinants of forest composition, we take into account regional level socio-economic and forest management variables as well. Our estimation results found that not only environmental factors but also socio-economic and forest management related factors strongly affect the composition of Korean forest. Based on the estimation results and IPCC scenarios on climate change, we predict that the share of currently dominant coniferous forest will decline in the future under all scenarios. About 10% of total forest area is likely to be converted from coniferous forest into broadleaved forest until 2090s under the scenario RCP 8.5. It is also predicted that there will be a substantial regional variation in the effects of climate change on forest composition, and the coniferous forests in the inland regions will decline more dramatically.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.77-77
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• 2017

현재 국내의 경우 4륜 승용관리기가 개발되었으나 성능 미비와 편이성 부족으로 승용관리기 및 부속 작업기 보급이 매우 저조한 상태이다. 3륜 승용관리기는 작업기 복부 장착으로 작업의 정밀도, 편이성, 관측의 용이성 향상과 함께 지상고 제고, 차폭 조절, 조향 등의 성능 개선을 기할 수 있다. 국내에서는 3륜 승용관리기용 복부 장착 부속작업기가 거의 개발되어 있지 않으며, 특히 두류 재배에 있어서 일관기계화 작업을 위한 관리작업기의 개발은 매우 필요한 실정이다. 승용관리기에 의한 중경제초는 잡초방제는 물론 물 빠짐과 토양 속 통기가 좋아지고, 지온 조절, 쓰러짐 방지, 부정근 발생을 조장하여 콩의 생육과 결실을 좋게 함으로써 수량이 증가되는 효과가 있다. 따라서 본 연구는 3륜 승용관리기에 적합한 복부장착형의 중경제초기를 설계 제작하였다. 3륜 승용관리기용 중경제초기는 승용관리기의 복부 PTO에서의 구동력 전달을 위한 연결 조인트, 중간축, 기어 및 체인 전동기구, 구동축으로 구성된 동력전달부, 중경제초를 위한 5개의 로타리 날과 후방 1개의 제초날로 구성된 3조의 작업날, 경심 조절을 위한 바퀴, 다양한 조간거리에 적응을 위한 조간조정 나사부, 흙의 비산 방지를 위한 로타리 날 커버 및 케이스, 3륜 승용관리기에의 부착을 위한 프레임 및 부착보조 작업기 이동용 바퀴장치 등으로 구성 설계 제작하였다. 로타리 날은 기존 시판 관리기용 로타리 중경 날을 사용하였는데 진행방향에 역으로 회전하도록 하였고, 날의 회전반경은 약 330 mm, 조당 경운 폭은 약 250 mm, 최대 경심 100 mm로 설계하였다. 조간거리의 조절범위는 수동나사에 의하여 620~900 mm 범위로 무단조절 할 수 있도록 하였다. 중경제초기는 3륜 승용 관리기 복부의 평행링크에 부착되므로 작업기의 이동 및 부착 편이성을 위하여 이동용 바퀴를 설치하였다. 제작된 중경제초기의 크기는 길이${\times}$폭${\times}$높이가 $660{\times}2072{\times}880mm$, 중량은 약 200 kg으로 제작되었다.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.76-76
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• 2017

4륜 승용관리기는 보통 후방이나 전방에 작업기를 장착하여 작업하나 3륜 승용관리기는 작업기를 복부에 장착함으로 작업의 정밀도, 편이성, 관측의 용이성 향상과 함께 지상고 제고, 차폭 조절, 조향 등의 성능 개선을 기할 수 있다. 국내에서는 3륜 승용관리기용 복부 장착 부속작업기가 거의 개발되어 있지 않으며, 특히 두류 재배에 있어서 일관기계화 작업을 위한 관리작업기의 개발이 매우 필요한 실정이다. 승용관리기의 중경배토기에 의한 작업은 기존 인력에 의한 작업보다 약 87%의 노력을 절감할 수 있으며, 잡초방제 효과도 인력에 비하여 크게 떨어지지 않고, 수량도 증가하는 것으로 보고되고 있다. 따라서 본 연구는 3륜 승용관리기에 적합한 복부장착형의 중경배토기를 설계 제작하였다. 3륜 승용관리기용 중경배토기는 승용관리기의 복부 PTO에서의 구동력 전달을 위한 연결 조인트, 중간축, 기어 및 체인 전동기구, 구동축으로 구성된 동력전달부, 4개의 로타리 날과 후방 1개의 배토날로 구성된 3조의 작업날, 로타리 날과 배토날의 경심 조절을 위한 3조의 바퀴, 흙의 비산 방지를 위한 로타리날 커버 및 케이스, 다양한 조간거리에 적응을 위한 조간조정 나사부, 3륜 승용관리기에의 부착을 위한 프레임 및 부착보조 작업기 이동용 바퀴장치 등으로 구성 설계 제작하였다. 로타리 날과 배토날은 기존 시판 관리기용 날을 사용하였다. 로타리 날은 진행방향으로 회전하도록 하였으며, 날의 회전반경은 약 330 mm, 조당 경운 폭은 약 230 mm 설계하였다. 최대 경심은 100 mm이며, 조간거리의 조절범위는 수동 나사에 의하여 620~900 mm 범위로 무단조절 할 수 있도록 하였다. 중경제초기는 3륜 승용 관리기 복부의 평행링크에 부착되므로 작업기의 이동 및 부착 편이를 위하여 이동용 바퀴를 설치하였다. 제작된 중경배토기의 크기는 길이${\times}$폭${\times}$높이가 $660{\times}2072{\times}880mm$, 중량은 약 200 kg으로 제작되었다.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.75-75
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• 2017

3륜 승용관리기는 4륜 승용관리기에 비하여 작업기를 복부에 장착함으로 작업의 정밀도, 편이성, 관측의 용이성 향상과 함께 지상고 제고, 차폭 조절, 조향 등의 성능 개선을 기할 수 있다. 국내에서는 3륜 승용관리기용 복부 장착 부속작업기가 거의 개발되어 있지 않으며, 특히 두류 재배에 있어서 일관기계화 작업을 위한 관리작업기의 개발이 매우 필요한 실정이다. 콩의 순 지르기 작업은 콩이 지나친 생육을 한 경우 미리 줄기의 윗부분을 제거하는 저심처리를 해주면 곁가지들의 왕성한 생육을 유도하여 많은 꼬투리를 얻고 쓰러짐도 줄어들어 수량을 높일 수 있다. 순 지르기 작업은 보통 낫이나 배부식 예취기를 이용하여 작업함으로 노동력이 많이 들고 작업부담이 심하여 개선이 필요한 실정이다. 따라서 본 연구는 3륜 승용관리기에 적합한 복부장착형의 순 지르기 작업기를 설계 제작하였다. 3륜 승용관리기용 순 지르기 작업기는 승용관리기의 복부 PTO에서의 구동력 전달을 위한 연결 조인트, 중간축, 기어 전동기구, 구동축으로 구성된 동력전달부, 2 조의 원판형 작업 날, 다양한 조간거리에 적응을 위한 조간조정 나사부, 3륜 승용관리기에의 부착을 위한 프레임 및 부착보조 작업기 이동용 바퀴장치 등으로 구성 설계 제작하였다. 원판형 칼날은 시판 중인 예취기의 외경 305 mm, 칼날 톱니 수 90개의 초경 비트 부착 날을 사용하였다. 작업 날의 승강높이는 150~540 mm 범위로 조절하도록 하였다. 조간거리의 조절범위는 수동나사에 의하여 660~860 mm 범위로 무단조절 할 수 있도록 하였다. 순 지르기 작업기는 3륜 승용 관리기 복부의 평행링크에 부착되므로 작업기의 이동 및 부착 편이를 위하여 이동용 바퀴를 설치하였다. 제작된 순 지르기 작업기의 크기는 길이${\times}$폭${\times}$높이가 $514{\times}1138{\times}810mm$, 중량은 약 100 kg으로 제작되었다.

• The Journal of Engineering Geology
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• v.23 no.4
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• pp.467-476
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• 2013

With increasing interest in environmental issues and the quality of surface water becoming inadequate for water supply, the Korean government has launched a groundwater development policy to satisfy the demand for clean water. To drive this policy effectively, it is essential to guarantee the accuracy of sustainable groundwater yield and groundwater use amount. In this study, groundwater use was monitored over several years at various locations in Korea (32 cities/counties in 5 provinces) to obtain accurate groundwater use data. Statistical analysis of the results was performed as a method for estimating rational groundwater use. For the case of groundwater use for living purposes, we classified the cities/counties into three regional types (urban, rural, and urban-rural complex) and divided the groundwater facilities into five types (domestic use, apartment housing, small-scale water supply, schools, and businesses) according to use. For the case of agricultural use, we defined three regional types based on rainfall intensity (average rainfall, below-average rainfall, and above-average rainfall) and the facilities into six types (rice farming, dry-field farming, floriculture, livestock-cows, livestock-pigs, and livestock-chickens). Finally, we developed groundwater-use estimation equations for each region and use type, using cluster analysis and regression model analysis of the monitoring data. The results will enhance the reliability of national groundwater statistics.

• Horticultural Science & Technology
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• v.31 no.3
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• pp.344-351
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• 2013

Microsatellite is one of the most suitable marker for cultivar identification as it has great discrimination power for cultivars with narrow genetic variation. The polymorphism level between 358 microsatellite primer pairs and 11 commercial cucumber cultivars was investigated. Thirty-one primer pairs showed high polymorphism within cucumber cultivars with different fruit types. These markers were applied for the constructing DNA profile data base of 110 commercial cucumber cultivars through multiplex PCR and fluorescence based automatic detection system. A total of 139 polymorphic amplified fragments were obtained by using 31 microsatellite markers. The average of PIC value was 0.610 ranging from 0.253 to 0.873. One hundred and thirty nine microsatellite loci were used to calculate Jaccard's distance coefficients for UPGMA cluster analysis. A clustering group of varieties, based on the results of microsatellite analysis, were categorized into plant shape and fruit type. Almost the cultivars were discriminated by marker genotypes. This information may be useful to compare through genetic relationship analysis between existing variety and candidate varieties in distinctive tests and protection of plant breeders' intellectual property rights through variety identification.

• Journal of Korean Society of Rural Planning
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• v.18 no.4
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• pp.13-26
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• 2012

The purpose of this study is to select the study area, which will be formed into Daegu Science Park as an national industrial complex, and to assess the landscape value based on biotop classification with different polygon forms, and to develop and computerize Biotop Value Assessment Tool (B-VAT) based on GIS. The result is as follows. First, according to the result of biotop classification based on an advanced analysis on preliminary data, a field study, and a literature review, total 13 biotop groups such as forrest biotop groups and total 63 biotop types were classified. Second, based on the advanced research on landscape value assessment model of biotop, we development biotop value assessment tool by using visual basic programming language on the ArcGIS. The first application result with B-VAT showed that the first grade was classified into 19 types including riverside forest(BE), the second grade 12 types including artificial plantation(ED), and the third class, the fourth grade, and the fifth grade 12 types, 2 types, and 18 types respectively. Also, according to the second evaluation result with above results, we divided a total number of 31 areas and 34 areas, which had special meaning for landscape conservation(1a, 1b) and which had meaning for landscape conservation(2a, 2b, 2c). As such, biotop type classification and an landscape value evaluation, both of which were suggested from the result of the study, will help to scientifically understand a landscape value for a target land before undertaking reckless development. And it will serve to provide important preliminary data aimed to overcome damaged landscape due to developed and to manage a landscape planning in the future. In particular, we expect that B-VAT based on GIS will help overcome the limitations of applicability for of current value evaluation models, which are based on complicated algorithms, and will be a great contribution to an increase in convenience and popularity. In addition, this will save time and improve the accuracy for hand-counting. However, this study limited to aesthetic-visual part in biotop assessment. Therefore, it is certain that in the future research comprehensive assessment should be conducted with conservation and recreation view.