• International Journal of Industrial Entomology and Biomaterials
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• 제31권1호
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• pp.14-19
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• 2015

In a previous report, we identified several candidate antimicrobial peptides through de novo RNA sequencing of the centipede Scolopendra subspinipes mutilans. Here, we identify and characterize one of these peptides, Scolopendrasin I. We identified the centipede antimicrobial peptide Cecropin from the centipede transcriptome using an SVM algorithm, and subsequently analyzed the amino acid sequence for predicted secondary structure using a GOR algorithm. We identified an alpha helical region of Cecropin and named it Scolopendrasin I. We then assessed antimicrobial and hemolytic activity of Scolopendrasin I. Scolopendrasin I showed antimicrobial activity against various microbes, including antibiotic-resistant Gram-negative bacteria, in a radial diffusion assay. Scolopendrasin I had potent antibacterial activity against acne-associated microbes in a colony count assay and showed no hemolytic activity in a hemolysis assay. In addition, we confirmed that Scolopendrasin I bound to the surface of bacteria via a specific interaction with lipoteichoic acid and lipopolysaccharide, two components of bacterial cell membranes. In conclusion, the results presented here provide evidence that this is an efficient strategy for antimicrobial peptide candidate identification and that Scolopendrasin I has potential for successful antibiotic development.

• 농촌계획
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• 제19권4호
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• pp.55-71
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• 2013

This study of the living environment for rural residents to identify the relationship between perception and satisfaction are as. Jeollabukdo rural residents survey and SPSS 18.0 statistical program utilizes improvement of rural environment and living conditions were discussed. The main analysis results are summarized as follows. First, rural residents use the facility convenience and accessibility, economic burden, there was a tendency to recognize the important, good natural environment or their children / relatives for the distance was a low awareness. Second, the location and land a job for men, the women have a higher awareness of the use of public transportation inclination showed less than 50 age group showed higher awareness. Third, the rural living environment of residents' satisfaction with 3.544, and overall satisfaction with village life showed a high propensity men have higher satisfaction than women. Finally, the living environment for the perception and satisfaction with the relationship between residential stability and economic awareness of a higher living environment was found that high satisfaction. These findings further improve the rural environment, improve satisfaction in that you can plan the basis for an effective strategy can be. More sustainable in the future, including the analysis of various parameters in progress by being linked to policy measures that will be provided.

• 농촌계획
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• 제8권1호
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• pp.94-104
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• 2002

In spite of the importance of the farm area in Seoul, in providing fresh vegetables, a pleasant environment and a good quality of life for residents, rapid urbanization and industrialization have greatly reduced the farm area. The purpose of this study is to examine farmers' intentions and attitudes to provide supporting data for planning the strategy of urban agricultural development. All the collected data was analyzed using the contingency tables and the Chi-square test using the SAS computer statistical package. Based on analysis of the survey data, the leaseholders were found to be more satisfied with their job than the landowning farmers. Also, the small-scale farmers with green houses showed greater job satisfaction than the ordinary large-scale farmers. Farmers' views on the farming in Seoul were different depending on their status. Therefore, agricultural strategies in there should be considered their different attitudes.

• The Plant Pathology Journal
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• 제20권3호
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• pp.229-233
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• 2004

The bacterial leaf blight, which is caused by Xantho-monas oryzae pv. oryzae, is the most damaging and intractable disease of rice. To identify the genes involved in the virulence mechanism of transposon TnS complex, which possesses a linearized transposon and transposase, was successfully introduced into X. oryzae pv. oryzae by electroporation. The transposon mutants were selected and confirm the presence of transposition in X. oryzae pv. oryzae by the PCR amplification of transposon fragments and the Southern hybridization using these mutants. Furthermore, transposon insertion sites in the mutant bacterial chromosome were deter-mined by direct genomic DNA sequencing using transposon-specific primers with ABI 3100 Genetic Analyzer. Efficiency of transposition was influenced mostly by the competence status of X. oryzae pv. oryzae cells and the conditions of electroporation. These results indicated that the insertion mutagenesis strategy could be applied to define function of uncharacterized genes in X. oryzae pv. oryzae.

• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.43-48
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• 2017

In a previous study, we analyzed the transcriptome of Scolopendra subspinipes mutilans using next-generation sequencing technology and identified several antimicrobial peptide candidates. One of the peptides, scolopendrasin V, was selected based on the physicochemical properties of antimicrobial peptides using a bioinformatics strategy. In this study, we assessed the antimicrobial activities of scolopendrasin V using the radial diffusion assay and colony count assay. We also investigated the mode of action of scolopendrasin V using flow cytometry. We found that scolopendrasin V's mechanism of action involved binding to the surface of microorganisms via a specific interaction with lipopolysaccharides, lipoteichoic acid, and peptidoglycans, which are components of the bacterial membrane. These results provide a basis for developing peptide antibiotics.

• 가정과삶의질연구
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• 제31권4호
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• pp.33-45
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• 2013

This paper proposes strategy development to improve the management of Multicultural Family Support Centers by analyzing four Multicultural Family Support Centers (MFSCs) in Chungcheongnam-do. The research was conducted from September 2012 to January 2013. Future directions for the four MFSCs are established based on a SWOT analysis on both the internal and external environments of the centers. Various strategic directions for the four MFSCs were identified based on these analyses. For center A (urban type, college contract type), the strategic directions included the specialization strategy, systemization strategy, empowerment strategy, standardization strategy, and achievement maximization strategy. The strategic directions for center B (urban-rural combined type, social welfare center contract type) included the improvement of multiculture awareness, specialization strategy, human resource development strategy, and networking strategy. For center C (farming and fishing community type, direct management type by local authority), the strategic directions included the specialization strategy, activation strategy, qualification strategy, and networking strategy. For center D (farming and fishing community type, social welfare center contract type), the strategic directions included the specialization strategy, business standardization strategy, human resource development strategy, and network expansion strategy. The suggested management strategy development for the four MFSCs can provide implications for the management of MFSCs in other areas in Korea.

• 한국지역사회생활과학회지
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• 제15권4호
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• pp.127-136
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• 2004

Local fairs are often part of the economic development strategy of rural areas. This study aims to find out marketing strategies for promoting the sales of regional specialty products through local festivals in rural Korea. The data were collected in late 2003 (from a sample of interviews and surveys from 351 visitors. The data were analyzed using the SPSS computer program. The results were as follows; First, the factors that visitors were considered in purchasing were quality (49.5％), commemorative value (36.9％), and reliability (33.0％). And the top reasons cited for not buying were lack of distinction (45.1％) and high prices (26.6％). The discount rates expected in markets was 16.8％. Second, 49.8％ of visitors has spent over 10,000 won and their purchase pattern has been for themselves and their family. Third, the public organizations for certi(ication for visitors were the National Agricultural Cooperative Fedration (37.9％) and the Ministry of Agriculture and Forestry (MAF) (22.5％). In conclusion, it was important for local stakeholders to provide more locally-manufactured, seasonal and traditional food, and to plan more locally characteristic events for their fairs. Further recommendations have included having a guide showing pricing and quality standards for products bought at rural fairs, developing product ideas for inducing impulse purchases, active promotion and marketing at event sites, and setting a standard system of warranties on products under the certification of the local authorities and with the financial support of the MAF Such recommendations were aimed at increasing the sale of traditional and regional specialty products at local festivals.

• 농업과학연구
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• 제37권3호
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• pp.523-534
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• 2010

This paper aims to develop a revitalized strategy for Daecheongdong district located on surrounding area of Daecheong dam, which general development projects are restricted by the acts of Green-belt area and Water supply protection zone. The study area consists of 13 villages in Donggu, Daejeon city. This study performed not only survey of amenity and human resources in the whole study area, but also development of new strategy integrating $1^{st}$, $2^{nd}$, and $3^{rd}$ industries for agriculture with potential. In the sample survey to 11% of village people, the new strategy was accepted positively with 88% of Understanding and 65% of Agreement in answer. The composition plans of farming association and total brand in the study area were agreed by 30% of residents with strong opinion. Representative groups of people living in Donggu, outside of the study area, have also positive opinion of 86% for Purchasing of agricultural products, 89% of Experience tourism, and 61% of Corporation for the study area, which the answering rate was 11% among 326 people. Finally, this study also investigated the corresponding Acts on performing of the new projects extracted in this study.

• Journal of Microbiology and Biotechnology
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• 제25권8호
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• pp.1275-1280
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• 2015

Previously, we performed de novo RNA sequencing of Scolopendra subspinipes mutilans using high-throughput sequencing technology and identified several antimicrobial peptide candidates. Among them, a cationic antimicrobial peptide, scolopendrasin VII, was selected based on its physicochemical properties, such as length, charge, and isoelectric point. Here, we assessed the anticancer activities of scolopendrasin VII against U937 and Jurkat leukemia cell lines. The results showed that scolopendrasin VII decreased the viability of the leukemia cells in MTS assays. Furthermore, flow cytometric analysis and acridine orange/ethidium bromide staining revealed that scolopendrasin VII induced necrosis in the leukemia cells. Scolopendrasin VII-induced necrosis was mediated by specific interaction with phosphatidylserine, which is enriched in the membrane of cancer cells. Taken together, these data indicated that scolopendrasin VII induced necrotic cell death in leukemia cells, probably through interaction with phosphatidylserine. The results provide a useful anticancer peptide candidate and an efficient strategy for new anticancer peptide development.

• The Plant Pathology Journal
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• 제20권4호
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• pp.240-243
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• 2004

White rot on Allium crops recently had a high incidence with incrensed cultivating areas of tropical garlic types in Korea. Two types of Sclerotium have known as causal agents that produce different size and shapes of sclerotia in infested fields. Therefore, we developed a new method for isolation of sclerotia from infested field soils that can be used for ecological study of Sclerotium spp. and establishment of control strategy. Soil samples collected from heavily infested fields were evenly mixed and placed on a automatic sieve shaker connected with tap water, After 10 min of shaking, residues on 0.5 mm and 0.25 mm sieves were separately collected and suspended with 70% sugar solution, which method floats sclerotia in aqueous layer, Then, floated fraction was carefully separated and mixed with a same volume of 1% sodium hypochlorite solution to differentiate with organic materials. This method provides a direct count of sclerotia under a dissecting microscopy.