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http://dx.doi.org/10.4014/jmb.1609.09057

Antimicrobial Activity of the Scolopendrasin V Peptide Identified from the Centipede Scolopendra subspinipes mutilans  

Lee, Joon Ha (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, In-Woo (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, Mi-Ae (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Ahn, Mi-Young (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Yun, Eun-Young (Graduate School of Integrated Bioindustry, Sejong University)
Hwang, Jae Sam (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Publication Information
Journal of Microbiology and Biotechnology / v.27, no.1, 2017 , pp. 43-48 More about this Journal
Abstract
In a previous study, we analyzed the transcriptome of Scolopendra subspinipes mutilans using next-generation sequencing technology and identified several antimicrobial peptide candidates. One of the peptides, scolopendrasin V, was selected based on the physicochemical properties of antimicrobial peptides using a bioinformatics strategy. In this study, we assessed the antimicrobial activities of scolopendrasin V using the radial diffusion assay and colony count assay. We also investigated the mode of action of scolopendrasin V using flow cytometry. We found that scolopendrasin V's mechanism of action involved binding to the surface of microorganisms via a specific interaction with lipopolysaccharides, lipoteichoic acid, and peptidoglycans, which are components of the bacterial membrane. These results provide a basis for developing peptide antibiotics.
Keywords
Antimicrobial peptide; membrane damage; bacterial membrane; scolopendrasin V; Scolopendra subspinipes mutilans;
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Times Cited By KSCI : 5  (Citation Analysis)
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