The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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A New Method for Sclerotial Isolation of Two Species of Sclerotium from Infested Soils

  • Kwon, Mi-Kyung (Plant Pathology Division, National Institute of Agricultural Science of Technology, Rural Development Administration) ;
  • Shim, Hong-Sik (Plant Pathology Division, National Institute of Agricultural Science of Technology, Rural Development Administration) ;
  • Yeh, Wan-Hae (Plant Pathology Division, National Institute of Agricultural Science of Technology, Rural Development Administration) ;
  • Kim, Taek-Soo (Plant Pathology Division, National Institute of Agricultural Science of Technology, Rural Development Administration) ;
  • Cho, Weon-Dae (Plant Pathology Division, National Institute of Agricultural Science of Technology, Rural Development Administration) ;
  • Kim, Choong-Hoe (Plant Pathology Division, National Institute of Agricultural Science of Technology, Rural Development Administration) ;
  • Kim, Yong-Ki (Plant Pathology Division, National Institute of Agricultural Science of Technology, Rural Development Administration)
  • Published : 2004.12.31
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Abstract

White rot on Allium crops recently had a high incidence with incrensed cultivating areas of tropical garlic types in Korea. Two types of Sclerotium have known as causal agents that produce different size and shapes of sclerotia in infested fields. Therefore, we developed a new method for isolation of sclerotia from infested field soils that can be used for ecological study of Sclerotium spp. and establishment of control strategy. Soil samples collected from heavily infested fields were evenly mixed and placed on a automatic sieve shaker connected with tap water, After 10 min of shaking, residues on 0.5 mm and 0.25 mm sieves were separately collected and suspended with 70% sugar solution, which method floats sclerotia in aqueous layer, Then, floated fraction was carefully separated and mixed with a same volume of 1% sodium hypochlorite solution to differentiate with organic materials. This method provides a direct count of sclerotia under a dissecting microscopy.

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References

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