• Journal of Information Processing Systems
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• v.20 no.2
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• pp.149-158
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• 2024

Traditional manual identification of crop leaf diseases is challenging. Owing to the limitations in manpower and resources, it is challenging to explore crop diseases on a large scale. The emergence of artificial intelligence technologies, particularly the extensive application of deep learning technologies, is expected to overcome these challenges and greatly improve the accuracy and efficiency of crop disease identification. Crop leaf disease identification models have been designed and trained using large-scale training data, enabling them to predict different categories of diseases from unlabeled crop leaves. However, these models, which possess strong feature representation capabilities, require substantial training data, and there is often a shortage of such datasets in practical farming scenarios. To address this issue and improve the feature learning abilities of models, this study proposes a deep transfer learning adaptation strategy. The novel proposed method aims to transfer the weights and parameters from pre-trained models in similar large-scale training datasets, such as ImageNet. ImageNet pre-trained weights are adopted and fine-tuned with the features of crop leaf diseases to improve prediction ability. In this study, we collected 16,060 crop leaf disease images, spanning 12 categories, for training. The experimental results demonstrate that an impressive accuracy of 98% is achieved using the proposed method on the transferred ResNet-50 model, thereby confirming the effectiveness of our transfer learning approach.

• Proceedings of The Korean Society of Health Promotion Conference
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• 1999.07a
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• pp.149-163
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• 1999

Department of Health Services, under the Ministry of Health, Nepal has adopted the five year plan and Primary Health Care service as the main strategy to provide basic health service at the rural areas. However, development of the Specialized Services in the Urban areas, Human Resource Development, Management Strengthening and Investment of Private and External Sector are also highly entertained through its Liberalization Policy. But due to, Early Marriage, Superstitious Traditional Beliefs ＆ behavior, Poor Sanitation of living, Poverty, Illiteracy, Lack of Supervision and Monitoring, High Density population in relation to arable land and Lack of Private and External Sector Investment, the Country is still suffering from high Fertility, Malnutrition, Infectious diseases and so high Death Rates. So Primary Health Care Services should be more emphasized than before; Community Financing, Private and External Sector should be highly involved; Manpower Development and Specialized Services should be most taken care; Management Skills be more strengthened and Evaluating the previous work and avoiding the mistake for the future program implementation should be well done. If these are improved, then the health will be definitely promoted to meet the Health Target of Nepal.

• Journal of Crop Science and Biotechnology
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• v.10 no.1
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• pp.50-56
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• 2007

Barley S-adenosylmethionine synthetase1 gene, which was differentially expressed in seed development of extra early barley, was regulated by the phytohormones and abiotic stresses. In order to identify the regulation regions which were involved in transcriptional control of the phytohormones and abiotic stresses, we isolated 1459 bp fragment of HvSAMS1 gene promoter using genome walking strategy and deletion series were constructed. Deleted upstream fragments(-1459, -1223, -999, -766, -545, -301 bp) were fused to the GUS reporter gene and evaluated via Agrobacterium-mediated transient expression assay. Increased GUS activity of HvSMAS1 promoter -301/GUS construct under each of NaCl, $GA_3$, ABA and ethylene application was found. However, GUS activity was negligible in the leaves transformed with the HvSMAS1 promoter(-1459, -1223, -999, -766 and -545)/GUS constructs. No significant induction of GUS activity was observed for the ethionine and spermidine treatments. In order to locate promoter sequence of the HvSAMS1 gene that was critical for the activation of gene expression, deletion and addition promoter derivatives(+, includes 43 bp of 5' ORF) of the HvSAMS1 gene fused to the GUS reporter gene were applied. The tobacco leaves which harbored the additional HvSAMS1 promoter(-1459+, -1459 to -546, -545+ and -301+)/GUS construct did not significantly induce GUS activity as compared to the HvSAMS1 promoter(-1459, -545 and -301)/GUS constructs under each of NaCl, ABA and $GA_3$ treatment. However, the GUS activity was high in the tobacco leaves which harboring the -211 to -141 regions of the HvSAMS1 promoter. This result suggested that HvSAMS1 gene expression might be regulated by this region(from -211 to -141).

• Journal of Digital Convergence
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• v.20 no.4
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• pp.147-157
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• 2022

This study tried to propose a smart farm technology strategy suitable for the domestic situation, focusing on the differentiation suitable for the domestic situation of ICT technology. In the case of advanced countries in the overseas agricultural industry, it was confirmed that they focused on the development of a specific stage that reflected the geographical characteristics of each country, the characteristics of the agricultural industry, and the characteristics of the people's demand. Confirmed that no enemy development is being performed. Therefore, in response to problems such as a rapid decrease in the domestic rural population, aging population, loss of agricultural price competitiveness, increase in fallow land, and decrease in use rate of arable land, this study aims to develop smart farm ICT technology in the future to create quality agricultural products and have price competitiveness. It was suggested that the smart farm should be promoted by paying attention to the excellent performance, ease of use due to the aging of the labor force, and economic feasibility suitable for a small business scale. First, in terms of economic feasibility, the ICT technology is configured by selecting only the functions necessary for the small farm household (primary) business environment, and the smooth communication system with these is applied to the ICT technology to gradually update the functions required by the actual farmhouse. suggested that it may contribute to the reduction. Second, in terms of performance, it is suggested that the operation accuracy can be increased if attention is paid to improving the communication function of ICT, such as adjusting the difficulty of big data suitable for the aging population in Korea, using a language suitable for them, and setting an algorithm that reflects their prediction tendencies. Third, the level of ease of use. Smart farms based on ICT technology for the development of the Industry6.0 (1.0(Agriculture, Forestry) + 2.0(Agricultural and Water & Water Processing) + 3.0 (Service, Rural Experience, SCM)) perform operations according to specific commands, finally suggested that ease of use can be promoted by presetting and standardizing devices based on big data configuration customized for each regional environment.

• Journal of Microbiology and Biotechnology
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• v.27 no.11
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• pp.2010-2018
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• 2017

Mixotrophic microalgal growth gives a great premise for wastewater treatment based on photoautotrophic nutrient utilization and heterotrophic organic removal while producing renewable biomass. There remains a need for a control strategy to enrich them in a photobioreactor. This study performed a series of batch experiments using a mixotroph, Chlorella sorokiniana, to characterize optimal guidelines of mixotrophic growth based on a statistical design of the experiment. Using a central composite design, this study evaluated how temperature and light irradiance are associated with $CO_2$ capture and organic carbon respiration through biomass production and ammonia removal kinetics. By conducting regressions on the experimental data, response surfaces were created to suggest proper ranges of temperature and light irradiance that mixotrophs can beneficially use as two types of energy sources. The results identified that efficient mixotrophic metabolism of Chlorella sorokiniana for organics and inorganics occurs at the temperature of $30-40^{\circ}C$ and diurnal light condition of $150-200{\mu}mol\;E{\cdot}m^{-2}{\cdot}s^{-1}$. The optimal specific growth rate and ammonia removal rate were recorded as 0.51/d and 0.56/h on average, respectively, and the confirmation test verified that the organic removal rate was $105mg\;COD{\cdot}l^{-1}{\cdot}d^{-1}$. These results support the development of a viable option for sustainable treatment and effluent quality management of problematic livestock wastewater.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.21 no.1
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• pp.55-71
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• 2018

To improve the ecological function of urban areas, the guideline for applying the Biotope Area Ratio to the Environmental Impact Assessment (EIA) was developed in 2005 and modified in the July, 2017. This study investigates whether the guideline has been actually practiced in the real world by searching reports including 648 cases of the Strategic Environmental Impact Assessment (SEIA) and 471 cases of the EIA. The results show that the 38% of SEIA and the 43% of EIA include sections about Biotope Area Ratio, and the 15% of SEIA and the 25 % of EIA are satisfied the threshold of the Biotope Area Ratio suggested by the guideline. The statistical analysis results show that this low level of practice was not improved through the modification of the guideline in 2017. This is because the guideline is forcibleness, its explanation is unclear, and stockholders' understanding of it lacks. In addition, lack of tracking management on SEIA and EIA also contributes to the low level of practice of the guideline. To promote the practice, the efforts to legislate and publicize the guideline are required.

• Mycobiology
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• v.50 no.5
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• pp.374-381
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• 2022

In the mating of filamentous basidiomycetes, dikaryotic mycelia are generated through the reciprocal movement of nuclei to a monokaryotic cytoplasm where a nucleus of compatible mating type resides, resulting in the establishment of two different dikaryotic strains having the same nuclei but different mitochondria. To better understand the role of mitochondria in mushrooms, we created four sets of dikaryotic strains of Lentinula edodes, including B2×E13 (B2 side) and B2×E13 (E13 side), B5×E13 (B5 side) and B5×E13 (E13 side), E8×H3 (E8 side) and E8×H3 (H3 side), and K3×H3 (K3 side) and K3×H3 (H3 side). The karyotypes and mitochondrial types of the dikaryotic strains were successfully identified by the A mating type markers and the mitochondrial variable length tandem repeat markers, respectively. Comparative analyses of the dikaryotic strains on the mycelial growth, substrate browning, fruiting characteristics, and mitochondrial gene expression revealed that certain mitochondria are more effective in the mycelial growth and the production of fruiting body, possibly through the activated energy metabolism. Our findings indicate that mitochondria affect the physiology of dikaryotic strains having the same nuclear information and therefore a selection strategy aimed at mitochondrial function is needed in the development of new mushroom strain.

• Molecules and Cells
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• v.38 no.9
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• pp.773-780
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• 2015

3D8 single chain variable fragment (scFv) is a recombinant monoclonal antibody with nuclease activity that was originally isolated from autoimmune-prone MRL mice. In a previous study, we analyzed the nuclease activity of 3D8 scFv and determined that a HeLa cell line expressing 3D8 scFv conferred resistance to herpes simplex virus type 1 (HSV-1) and pseudorabies virus (PRV). In this study, we demonstrate that 3D8 scFv could be delivered to target tissues and cells where it exerted a therapeutic effect against PRV. PRV was inoculated via intramuscular injection, and 3D8 scFv was injected intraperitoneally. The observed therapeutic effect of 3D8 scFv against PRV was also supported by results from quantitative reverse transcription polymerase chain reaction, southern hybridization, and immunohistochemical assays. Intraperitoneal injection of 5 and $10{\mu}g$ 3D8 scFv resulted in no detectable toxicity. The survival rate in C57BL/6 mice was 9% after intramuscular injection of 10 $LD_{50}$ PRV. In contrast, the 3D8 scFv-injected C57BL/6 mice showed survival rates of 57% ($5{\mu}g$) and 47% ($10{\mu}g$). The results indicate that 3D8 scFv could be utilized as an effective antiviral agent in several animal models.

• Journal of Korea Technology Innovation Society
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• v.19 no.1
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• pp.1-28
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• 2016

Current Korean innovation system is facing a new turning point while the growth-oriented and S&T provide-oriented development strategy. Accordingly, there are needs for not only system transition in various area, such as sustainable energy, agriculture, and rural area but also STI policy paradigm shift to create a new innovative pathway. Living lab is being discussed in European country as a new innovative model based on user participation and as a niche experiments for sustainable system transition. This study attempts to analyze the living lab cases which are for the purpose of energy transition, agriculture rural areas system transition, and STI policy paradigm shift. Based on this analysis, the implications were derived in Korea. European Suslab project, C@R project, and Taiwan living lab, promoted diverse transitional experiments successfully by collecting users' background and experiences from pre-planning stage and by maintaining the user-driven innovative actions within the whole development process. This result provides various suggestions to current Korean situation that central government and local governments are considering the introduction of living lab. Living lab can also be utilized as a strategic niche experiments for socio-technical system transition in region or country, as a mean of policy integration, and as a new regional innovation model. In addition, it can be an important platform to realize the policy integration reflecting the user and demand-side which are highlighted in recent innovative policy paradigm.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.11
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• pp.833-842
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• 2020

The purpose of this study is to analyze the economic feasibility of technology developed by the National Institute of Animal Science used to shorten the raising term of Hanwoo cattle. This technology can address the increasing proportion of feed cost to operating expenses in the industry. The technology maintains quality by high nutrition breeding during the growing period while shortening the fattening period compared to conventional methods for castrated cattle. Our analysis employs the partial budget method, and results are as follows. First, we found that the ratio of shipment in age of less than 29 months has been increasing. Second, the statistical test finds that both the reduction in age of the months for shipment and the increase in dressed weight annually are significant. Third, the benefit of introducing the technology with the self-manufactured TMR accounts for 467,990 won per head/time. This result is strong evidence for prioritizing and extending pilot projects. In order to further expand this technology in the future, problems, limitations, and obstacles to introduction should be additionally disclosed for participating and non-participating farms after conducting the pilot project.