• Asian-Australasian Journal of Animal Sciences
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• v.25 no.6
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• pp.818-823
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• 2012

Tall fescue (Festuca arundinacea Schreb.) is an important cool season forage plant that is not well suited to extreme heat, salts, or heavy metals. To develop transgenic tall fescue plants with enhanced tolerance to abiotic stress, we introduced an alfalfa Hsp23 gene expression vector construct through Agrobacterium-mediated transformation. Integration and expression of the transgene were confirmed by polymerase chain reaction, northern blot, and western blot analyses. Under normal growth conditions, there was no significant difference in the growth of the transgenic plants and the non-transgenic controls. However, when exposed to various stresses such as salt or arsenic, transgenic plants showed a significantly lower accumulation of hydrogen peroxide and thiobarbituric acid reactive substances than control plants. The reduced accumulation of thiobarbituric acid reactive substances indicates that the transgenic plants possessed a more efficient reactive oxygen species-scavenging system. We speculate that the high levels of MsHsp23 proteins in the transgenic plants protect leaves from oxidative damage through chaperon and antioxidant activities. These results suggest that MsHsp23 confers abiotic stress tolerance in transgenic tall fescue and may be useful in developing stress tolerance in other crops.

• Journal of the Korean Society of Food Culture
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• v.37 no.6
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• pp.495-502
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• 2022

This study aimed to investigate the need for dietary education and programs for young Koreans belonging to single-person households in the metropolitan area. A total of 500 young adults aged 19-34 participated in the study through an online survey. Participants responded to questions on general characteristics, dietary problems, and the need for dietary education and programs. The subjects were divided into three groups as follows: Undergraduate students, employed workers, and others. Among the subjects from single-person households, 20.2, 67, and 12.8% were undergraduate students, employed workers, and others, respectively. When asked for their estimation of an appropriate self-pay when participating in a cooking class, 39.8% of total subjects responded '5,000-10,000 won'. The most preferred program for young adults in single-person households was the 'support food package'. For the preferred method of dietary education, undergraduate students showed a greater preference for classes 'at campuses'. However, employed workers and others had a higher preference for 'non-face-to-face online classes'. Undergraduate students tended to generally have a higher preference for dietary education and support programs compared to employed workers and others. This study provides data that will be useful for establishing healthy dietary policies and education programs for young single-person households in Korea.

• Journal of Korean Society of Rural Planning
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• v.26 no.2
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• pp.39-49
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• 2020

This study was attempted to grasp the push-pull factors of urban to rural migrants in relation to services and infrastructure of rural welfare and culture. Online and offline survey were conducted for urban residents who were willing to return to the rural area and those who were already returning to the rural area. In the fields of health care, social welfare, and education, it was found that the satisfaction level of urban-to-rural migrants was relatively higher than those of latent urban-to-rural migrants. In the field of culture, leisure and sports, the level of satisfaction and concern were similar, so it was found that the expectations for the field were relatively high before returning rural area. As for the agreement degree to expand support, the demand for emergency medical facilities and dementia care program was the highest, and the demand for health care was found to be relatively high. The results of the survey on the top priority items indicate that latent urban-to-rural migrants require support of facility and space item and expansion of program item, and urban-to-rural migrants have high demand for expansion of program item in all field except healthcare field. The results of this study are expected to provide useful information for establishing the direction of the rural area development project in connection with the revitalization of policy of people return to rural area.

• Proceeding of Spring/Autumn Annual Conference of KHA
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• 2011.04a
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• pp.176-181
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• 2011

This study has purpose to make a report on the newly established rural villages in this country, and to find out the success factors of the rural village project focusing the developers who succeeded in the sales of rural village with emphasized community function. For this study, 123 projects informed from the website(www.welchon.com) were analyzed on the frequency and the officials in charge were interviewed by telephone on the step of projects. In addition, 4 personnel of development companies answered the semi-structured questionnaire on the location, development theme, preparative resident meeting, and success factors of 7 projects which was dealt with content analysis. The result was as follows. 1) Among the projects, more than 50% were the detached housing type with less than 50 units, and also were the resident-driven development projects. Only 5 projects were completed. 2) The personnel of development companies pointed at the location, environment for the economic activity, project theme and continuous resident meeting, as success factors of project. The barriers for rural village development policy are inadequate regulation and long administrative procedure. The preparative resident meeting is an important success factor, so that the system and the cost to run a professional program have to be guaranteed.

• Journal of Applied Biological Chemistry
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• v.59 no.2
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• pp.159-164
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• 2016

Plant growth regulator is an essential pesticide to date while the available active ingredient is not well understood unlike fungicide, insecticide and herbicide. This study was aimed to evaluate a new chemical class of plant growth regulator, and the total of 92 benzene derivatives were screened for their germination and early stage of the root growth regulation on Brassica campestris. Thirty benzaldehydes, nine acids, one amide, and one ester showed potent root growth inhibitory activity (>70 % inhibition) while only salicylaldehyde showed potent germination inhibition ($IC_{50}=81.2mg/L$) suggesting that benzaldehyde was a key module candidate for the growth inhibition. Benzaldehydes were further evaluated for root growth inhibition. 2,3-Dihydroxybenzaldehyde and salicylaldehyde showed $IC_{50}$ values of 8.0 and 83.9 mg/L, respectively. On the other hand, salicylaldehyde, and 2,4,5-trihydroxybenzaldehyde were found to have root growth promotion effects less than 10 mg/L. This result suggests that the benzaldehyde is a new class candidate for plant growth regulator.

• Journal of Agricultural Extension & Community Development
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• v.16 no.1
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• pp.1-20
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• 2009

The purpose of this study is to evaluate an education program to take care of the elderly in the rural area. The education program that includes 4 sessions are carried out in the community center. The 4 sessions are to understand rural elderly and volunteer role, help elderly's daily living, aid elderly to use welfare services and support the elderly's leisure. The subjects were 30 persons under 60 years of age living in Jangseong and Hoengsung villages. By paired t-test, knowledge on caring and the attitude for elderly and volunteer showed significant differences between before and after the education program. After the training, 66.7% of the subjects answered 'yes' to the question that asked whether they would like to participate in caring volunteer for neighboring elderly. Subjects's positively changed attitude can stimulate positive behavior that eventually enables them to provide high quality services. The satisfaction of the program showed high scores for contents, place and operation of education.

• The Korean Journal of Community Living Science
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• v.19 no.3
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• pp.353-363
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• 2008

Recently some Korean feminists have reported the family management agreement (FMA) can contribute to enhance the women farmers' legal status and to improve their farm management. The FMA is a formal document written each rights and responsibilities on the farm management, income allocation, and labor condition such as working time and work-off days agreed among family members. Since 2004, 161 farmer couples have signed and practiced the FMA after two-day workshop scheduled for the agreement. The purpose of this study is to analyze the effects of FMA on the increase of participation in (am management among women farmers. Data were collected from 204 FMA couples through the structured questionnaires. Also interview and participating observation were carried out. Paired t-test were conducted using SPSS 12.0 for Windows program. Major findings were as follows. Women farmers' participation in decision making of farming activities has significantly increased after than before the FMA. However, husbands' participation in decision making is higher than women farmers even after the FMA. After the FMA, labor compensation, regularly or irregularly, for women farmers has increased to 53.3%, 35.3% higher than 18% of the previous study and women farmers' ownership of their farm assets has increased to 48.1% from 29.1%. Also, there is a tendency women farmers' role as a representative of their farm has increased. In conclusion the FMA makes women farmers participate more in farm management as decision maker, income beneficiary, farm asset owner, and farm representative, which encourage them to get the legal status as a farmer and to control their farm management risk. It is suggested that the FMA be selected as a program in order to strengthen the agricultural competitiveness.

• Journal of Applied Biological Chemistry
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• v.50 no.2
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• pp.63-69
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• 2007

Compositional components such as isoflavone, protein, oil, fatty acid, and free sugar in Korean vegetable soybeans were examined with four cultivars including Hwaeomputkong, Keunolkong, Mirang, and Danmi 2. In the isoflavone, Mirang cultivar showed the highest content ($967.1{\mu}g/g$), whereas Keunolkong was the lowest content ($535.9{\mu}g/g$). The malonylglucosides were the predominant isoflavone type followed by the glucoside, aglycone, and acetyl glucoside forms, respectively. In the protein content, Hwaeomputkong was the lowest (41.7%) and Danmi 2 was the highest (45.9%). The oil contents were 11.5 to 21.2% and Mirang cultivar was the lowest. The fatty acid compositions of the oil extracts exhibited that linoleic acid was the highest (33.6-42.5%), followed by oleic, palmitic, linolenic, and stearic acids. Whereas, oleic acid ($46.7{\pm}2.0%$) was more than linoleic acid ($33.6{\pm}1.3%$) in Mirang cultivar. In the free sugar contents, Hwaeomputkong cultivar showed the highest level and sucrose ($5.52{\pm}0.49%$) appeared to be most prevalent in vegetable soybeans.

• Molecules and Cells
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• v.28 no.2
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• pp.131-137
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• 2009

Plant defensins are small (5-10 kDa) basic peptides thought to be an important component of the defense pathway against fungal and/or bacterial pathogens. To understand the role of plant defensins in protecting plants against the brown planthopper, a type of insect herbivore, we isolated the Brassica rapa Defensin 1 (BrD1) gene and introduced it into rice (Oryza sativa L.) to produce stable transgenic plants. The BrD1 protein is homologous to other plant defensins and contains both an N-terminal endoplasmic reticulum signal sequence and a defensin domain, which are highly conserved in all plant defensins. Based on a phylogenetic analysis of the defensin domain of various plant defensins, we established that BrD1 belongs to a distinct subgroup of plant defensins. Relative to the wild type, transgenic rices expressing BrD1 exhibit strong resistance to brown planthopper nymphs and female adults. These results suggest that BrD1 exhibits insecticidal activity, and might be useful for developing cereal crop plants resistant to sap-sucking insects, such as the brown planthopper.

• The Plant Pathology Journal
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• v.32 no.1
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• pp.58-64
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• 2016

Bacterial wilt of tomatoes caused by Ralstonia solanacearum is a devastating disease that limits the production of tomato in Korea. The best way to control this disease is using genetically resistant tomato plant. The resistance degree to R. solanacearum was evaluated for 285 tomato accessions conserved in the National Agrobiodiversity Center of Rural Development Administration. These accessions of tomato were originated from 23 countries. Disease severity of tomato accessions was investigated from 7 days to 14 days at an interval of 7 days after inoculation of R. solanacearum under greenhouse conditions. A total of 279 accessions of tomato germplasm were susceptible to R. solanacearum, resulting in wilt and death in 70 to 90% of these plants. Two tomato accessions were moderately resistant to R. solanacearum. Only four accessions showed high resistance against R. solanacearum. No distinct symptom of bacterial wilt appeared on the resistant tomato germplasms for up to 14 days after inoculation of R. solanacearum. Microscopy of resistant tomato stems infected with R. solanacearum revealed limited bacterial spread with thickening of pit membrane and gum production. Therefore, these four resistant tomato germplasms could be used in tomato breeding program against bacterial wilt.