• BMB Reports
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• v.47 no.7
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• pp.382-387
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• 2014

Corn silk (CS) has long been consumed as a traditional herb in Korea. Maysin is a major flavonoid of CS. The effects of maysin on macrophage activation were evaluated, using the murine macrophage RAW 264.7 cells. Maysin was isolated from CS by methanol extraction, and preparative $C_{18}$ reverse phase column chromatography. Maysin was nontoxic up to $100{\mu}g/ml$, and dose-dependently increased TNF-${\alpha}$ secretion and iNOS production by 11.2- and 4.2-fold, respectively, compared to untreated control. The activation and subsequent nuclear translocation of NF-${\kappa}B$ was substantially enhanced upon treatment with maysin ($1-100{\mu}g/ml$). Maysin also stimulated the phosphorylation of Akt and MAPKs (ERK, JNK). These results indicated that maysin activates macrophages to secrete TNF-${\alpha}$ and induce iNOS expression, via the activation of the Akt, NF-${\kappa}B$ and MAPKs signaling pathways. These results suggest for the first time that maysin can be a new immunomodulator, enhancing the early innate immunity.

• Korean Journal of Agricultural Science
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• v.50 no.3
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• pp.357-364
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• 2023

In this study, we propose a ripe tomato bunch localization method based on convolutional neural networks, to be applied in robotic harvesting systems. Tomato images were obtained from a smart greenhouse at the Rural Development Administration (RDA). The sample images for training were extracted based on tomato maturity and resized to 128 × 128 pixels for use in the classification model. The model was constructed based on four-layer convolutional neural networks, and the classes were determined based on stage of maturity, using a Softmax classifier. The localization of the ripe tomato bunch region was indicated on a class activation map. The class activation map could show the approximate location of the tomato bunch but tends to present a local part or a large part of the ripe tomato bunch region, which could lead to poor performance. Therefore, we suggest a recursive method to improve the performance of the model. The classification results indicated that the accuracy, precision, recall, and F1-score were 0.98, 0.87, 0.98, and 0.92, respectively. The localization performance was 0.52, estimated by the Intersection over Union (IoU), and through input recursion, the IoU was improved by 13%. Based on the results, the proposed localization of the ripe tomato bunch area can be incorporated in robotic harvesting systems to establish the optimal harvesting paths.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.35 no.1
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• pp.203-215
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• 2015

The study aims to identify the degree and types of spatial recessions in Si/Gun and Eup/Myun units within Chungcheong region in South Korea to contribute to the efforts being made to diagnose the rural recession and the potentials. To this end, we analyzed 27 Sis and Guns to identify the degree of recession and potentials of rural areas in Chungcheong region. We also carried out the diagnosis and K-Means Clustering on 274 Eups and Myuns, smaller administrative units, to figure out the types and characteristics of the rural recessions. In case of the analysis targeting the Sis and Guns, a relatively high degree of rural recession was found in Cheongyang, Seocheon and Taean for Chungcheongnam-do, and in Danyang and Goisan, as well as in Boeun, Okcheon and Youngdong - which are collectively called as 'Southern 3 Areas in Chungcheongbuk-do' as they are conventionally known by their high degree of rural recession. According to the results of the clustering analysis carried out on the 166 Eups and Myuns, there were five outstanding clusters. They were; areas with housing deterioration (29), areas with poor economic foundation (16), areas with poor accessibility to central areas (42), areas with poor residential environment (51) and areas with aged population (28). The findings and results of the present study are likely to serve as a basis for the design and enforcement of forthcoming rural area activation policies. Also, it would be highly recommended that a more comprehensive diagnosis is taken from a community-level perspective and policy suggestions and strategies tailored for rural communities are further discussed.

• Journal of Embryo Transfer
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• v.27 no.3
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• pp.183-187
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• 2012

Adult stem cell transplantation has been increased every year, because of the lack of organ donors for regenerative medicine. Therefore, development of reliable and safety cryopreservation and bio-baking method for stem cell therapy is urgently needed. The present study investigated safety of dimethyl sulfoxide (DMSO) such as common cryoprotectant on porcine bone marrow derived mesenchymal stem cells (pBM-MSCs) by evaluating the activation of Caspase-3 and -7, apoptosis related important signal pathway. pBM-MSCs used for the present study were isolated density gradient method by Ficoll-Paque Plus and cultured in A-DMEM supplemented 10% FBS at $38.5^{\circ}C$ in 5% $CO_2$ incubator. pBM-MSCs were cryopreserved in A-DMEM supplemented either with 5%, 10% or 20% DMSO by cooling rate at $-1^{\circ}C$/min in a Kryo 360 (planner 300, Middlesex, UK) and kept into $LN_2$. Survival rate of cells after thawing did not differ between 5% and 10% DMSO but was lowest in 20% DMSO by 0.4% trypan blue exclusion. Activation of Caspase-3 and -7 by Vybrant FAM Caspase-3 and -7 Assay Assay Kit (Molecular probes, Inc.OR, USA) was analyzed with a flow cytometer. Both of cryopreserved and control groups (fresh pBM-MSCs) were observed after the activation of Caspase-3 and -7. The activation did not differ between 5% and 10% DMSO, but was observed highest in 20% DMSO. Therefore 5% DMSO can be possibly used for cell cryopreservation instead of 10% DMSO.

• Journal of the Korean Academic Society of Industrial Cluster
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• v.3 no.1
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• pp.1-10
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• 2009

Rural Industrial Complex(RIC) is considered as one of the most important sources of off-farm income in Korea. But RICs has many problems since it was beginning development. In 2008, RICs in Gyeongsangbuk-do are 56 place, and are 836 corportations in RICs. Employee is total 20,109 persons. Competitive power of RICs weak location condition, small business, lack of infrastructure, institute. The main focus of this paper is to derive policy suggestion for Gyeongsangbuk-do RICs. We investigate the operation status of Gyeongsangbuk-do RICs. It is necessary that RICs be promoted in light of revitalization program.

• Journal of Korean Society of Rural Planning
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• v.20 no.2
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• pp.81-90
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• 2014

This study was carried out to identify the limitations and problems of organization, as well as present plans to activate the organization through diagnosis on 'My Hometown Keeper' organization that was created for the purpose of growth of agricultural and fishing regions, and environment improvement. Utilizing Weisbord's Six-box Model, an organization diagnosis model useful for diagnosis of 'My Hometown Keeper' organization, 6 areas including organization's objective, structure, relationship, compensation system, leadership, subsidiary device system etc. and 14 survey questions were prepared, and a survey investigation was conducted on the staff at Korea Rural Community Corporation in charge of 'My Hometown Keeper' participating residents and administrative support. Based on the analysis results of survey investigation, the limitations and problems of organization were identified, and as plans to improve these and activate 'My Hometown Keeper' organization, 'Clear establishment of organization's objective and role', 'Preparation of compensation and incentive system', 'Growth of relationship and leadership between constituents' 'Enhancing the utilization of subsidiary device system such as education and information acquisition etc.' etc. were presented.

• Journal of Korean Society of Rural Planning
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• v.26 no.1
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• pp.13-22
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• 2020

This study analyzed the relationship between the sense of community, organizational commitment, and business performance among farmers participating in the agricultural products processing center. As a result, the farmer's sense of community at the agricultural products processing center had a positive effect on organizational commitment, and the farmer's sense of community had a positive effect on business performance. In this regard, efforts should be made to systematically manage and sustain the future of formal, formal and informal activities of the agricultural products processing center. In addition, in order for the community formed at the agricultural products processing center to continue to grow, it is necessary to take the approach of improving the competence of personnel, organizing work, developing professional programs, and forming related networks. In addition, the organization formed through the agricultural products processing center should be grown as a farmer's organization of processing base so that it can be linked with the activation policy such as food plan and local food.

• Journal of Korean Society of Rural Planning
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• v.15 no.1
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• pp.101-107
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• 2009

The purposes of this study were to search for the ways to activate the Gochang Blackberry Festival by examining the visitors to the festival and utilizing the results to make efficient use of the local resources and vitalize the area. The results indicate that the visitors were aware of Gochang blackberry's superiority to that of other regions in quality and commercial value but lacked in the recognition of its related products, which suggests that the Gochang government should make diverse efforts to heighten their recognition of the products. There was a great demand for improving and supplementing the local festivals ad experience programs related to Gochang blackberry. It's thus required to reinforce new experience programs and develop various programs by incorporating blackberry into other local resources. The study has its limitations since its subjects were restricted to the visitors of the Gochang Blackberry Festival and it failed to investigate the preference factors of general potential tourists with the small sample. In future study, they need to use a bigger sample of general public to generalize the research findings.

• Journal of Korean Society of Rural Planning
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• v.23 no.4
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• pp.91-98
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• 2017

The purpose of this study is to analyze the actual condition of the already established rural theme park, draw the weak and strong points, and come up with an efficient performance plan for Changwon Persimmon Theme Park through the benchmarking of the best overseas case. According to the analysis, it is necessary to introduce a facility and a program for family customers as a plan for promoting Changwon Persimmon Theme Park. It is required to secure safety through public operation and management, improve scalability of the facility through the establishment of multi-functional complex facility, and come up with a plan for operating a special program associated with local resources. Therefore, this study drew three activation factors: the establishment of operation and management system, the development of operation contents by facility, and the establishment of operation in each area. First, to establish the operation and management system, it is necessary to classify the organization of the theme park into the operation business area and into operation & management area by job function. Secondly, it is necessary to establish a special program in consideration of space and facility, suggest a differentiated plan for operation contents, and create a facility operation plan and program for Changwon Persimmon Theme Park. Lastly, to operate and manage Changwon Persimmon Theme Park smoothly, it is necessary to set the theme park space as open collaboration space which the local residents can share and participate in by avoiding the conventional administration-based business performance and operation. This study suggested the plan for establishing an operation and management system for Changwon Persimmon Theme Park and its operation, the establishment of a special program, a business performance strategy, and the use of internal and external spaces of the theme park.

• Journal of Korean Society of Rural Planning
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• v.27 no.4
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• pp.25-32
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• 2021

This study was to analyze the Physical Activity Therapy Programs (PATPs) in U rban-rural region before and after COVID-19 in order to suggest a way of activating program. The subjects were the 43 PATPs performed in 4 Community Health Centers of Gimcheon, Jeongeup, and Pyeongtaek. The basic data was collected by official documents, expenditure budget, the homepage of the centers, national information disclosure portal, telephone interview, and e-mail with the person in charge of the programs. All the data were classified to the administrative districts, the health-related fitness variables, and the life cycles. The American College Sports Medicine Guidelines were used to evaluate the PATPs. As a results, the number of the PATPs was too small compared to the population of the regions. Also, the PATPs were not considered to the characteristics of participants such as Life-Cycle and regional facilities so on. The organization and management of the PATPs were principally deficient in improving health-related fitness variables. In 2020 as the period of COVID-19 pandemic, the number of programs and participants with face-to-face PATPs was significantlry decreased compared to 2019, while that was increased with non-face-to-face PATPs. In conclusion, PATPs should be increased and operated in accordance with scientific exercise prescription guidelines. Also, the programs should be considered with the proportion and characteristics of Life-Cycle population. Further, the various with non-face-to-face PATPs should be developed and screened with based on scientific data for post-corona virus pandemic. Further, non-face-to-face PATPs programs should include a kind of practical way to promote the individual physical activity.