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http://dx.doi.org/10.5483/BMBRep.2014.47.7.191

Immunostimulating activity of maysin isolated from corn silk in murine RAW 264.7 macrophages  

Lee, Jisun (Department of Biotechnology, The Catholic University of Korea)
Kim, Sun-Lim (National Institute of Crop Science, Rural Development Administration)
Lee, Seul (Department of Biotechnology, The Catholic University of Korea)
Chung, Mi Ja (Department of Food Science and Nutrition, College of Health, Welfare and Education, Gwangju University)
Park, Yong Il (Department of Biotechnology, The Catholic University of Korea)
Publication Information
BMB Reports / v.47, no.7, 2014 , pp. 382-387 More about this Journal
Abstract
Corn silk (CS) has long been consumed as a traditional herb in Korea. Maysin is a major flavonoid of CS. The effects of maysin on macrophage activation were evaluated, using the murine macrophage RAW 264.7 cells. Maysin was isolated from CS by methanol extraction, and preparative $C_{18}$ reverse phase column chromatography. Maysin was nontoxic up to $100{\mu}g/ml$, and dose-dependently increased TNF-${\alpha}$ secretion and iNOS production by 11.2- and 4.2-fold, respectively, compared to untreated control. The activation and subsequent nuclear translocation of NF-${\kappa}B$ was substantially enhanced upon treatment with maysin ($1-100{\mu}g/ml$). Maysin also stimulated the phosphorylation of Akt and MAPKs (ERK, JNK). These results indicated that maysin activates macrophages to secrete TNF-${\alpha}$ and induce iNOS expression, via the activation of the Akt, NF-${\kappa}B$ and MAPKs signaling pathways. These results suggest for the first time that maysin can be a new immunomodulator, enhancing the early innate immunity.
Keywords
Corn silk; Immunostimulating activity; iNOS; MAPK; Maysin; TNF-${\alpha}$;
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