• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.268-270
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• 2005

This study was carried out to investigate the antimicrobial activity of Rumex crispus development of antibiotics derived from natural products. To confirm antimicrobial activity, paper disc method and growth inhibition in liquid culture were applied. Antimicrobial activity was observed in Saccharomyces cerevisiae, Vibrio vulificus, Escherichia coli, Bacillus cereus, Staphylococcus aureus, and Cαndida bombicola.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.369.2-369.2
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• 2002

Rumex crispus (Polygonaceae) is a well known perennial plant. which is called So-Ri-Jaeng-Yi. growing in the field and on the roadside. It has been used as a Korean Folk medicine in treating of acute and chronic cutaneous disease. cathartics, fever and jaundice. Also, the seed of this plant has been used as only a folk medicine for the treatment of digestion problems, liver diseases and many sorts of tumor. So we examined analgesic activity, anti-inflammatory activities and hepatoprotective activity using MeOH extraction and BuOH fraction in this plant. (omitted)

• International Journal of Industrial Entomology and Biomaterials
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• 제23권2호
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• pp.215-221
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• 2011

To establish an indoor-rearing system for the Large Copper butterfly, $Lycaena$ $dispar$ and the Small Copper butterfly, $Lycaena$ $phlaeas$, the effect of temperature, photoperiod and host plants on larval development was investigated. The larvae of $Lycaena$ $dispar$ fed on $Rumex$ $crispus$, whereas the larvae of $Lycaena$ $phlaeas$ ate both $Rumex$ $crispus$ and $Rumex$ $acetosa$. The duration of the larval period of $Lycaena$ $dispar$ was 13.8 days on $R.$ $crispus$ and that, of $Lycaena$ $phlaeas$ was 15.9 days and 15.2 days on $R.$ $acetosa$ and $R.$ $crispus$ respectively. Laboratory experiments show that the Large Copper larvae are able to feed on other $Rumex$ species without harming their overall survival and can utilize these alternative host plants at least as efficiently as their natural host plant. This result suggests that plant chemistry is not responsible for their lack of utilization of the alternative host plants in the wild. Host plant choice by ovipositing females was measured with the two alternative hosts. $Lycaena$ $dispar$ preferred $R.$ $crispus$ to $R.$ $acetosa$, wheareas $Lycaena$ $phlaeas$ preferred $R.$ $acetosa$ to $R.$ $crispus$. Temperature has been proposed as an important determinant of developmental rate, lifespan and mortality in invertebrates. As temperature increased, length of the developmental period gradually decreased. The developmental period of the Large Copper larvae was 11.0 days and 28.5 days at $30^{\circ}C$ and $17.5^{\circ}C$, respectively.

• Korean Chemical Engineering Research
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• 제44권1호
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• pp.81-86
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• 2006

국내에 널리 자생하고 있는 소리쟁이를 이용하여 식품첨가제나 의약품으로 이용가능성을 탐색하고자 항균 항산화 활성에 대한 연구를 수행하였다. 소리쟁이 추출물에 의한 항균활성은 시험 균주 중 Pseudomonas aeruginosa를 제외한 모든 시험 균주에 대하여 항균활성을 나타내었고, 특히, Vibrio vulificus와 Saccharomyces cerevisiae에 대해서 상당한 항균효과를 나타내었다. 소리쟁이의 추출물을 DPPH 라디칼 소거 활성법을 이용하여 항산화활성을 측정한 결과, 에틸아세테이트 분획에서 항산화제로 사용되고 있는 BHA나 아스코르브산과 비슷한 항산화활성을 나타내었다. 소리쟁이 추출물 분획의 항산화활성과 페놀성 화합물 함량과의 상관관계를 조사한 결과, 에틸 아세테이트 분획에서 가장 많은 양의 페놀성 화합물이 함유되어 있었으며, 추출물의 항산화 활성은 페놀성 화합물의 함량과 밀접한 관계가 있는 것으로 사료된다.

• 한국약용작물학회지
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• 제27권3호
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• pp.208-217
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• 2019

Background: In the present study, we identified two cytotoxic compounds from the root of Rumex crispus L. using a bioassay-based method. Methods and Results: Compared with the other fractions, the diethyl ether ($Et_2O$) fraction of R. crispus root extract exhibited the strongest of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging effect [scavenging concentration 50% $(SC_{50})=63.8{\pm}1.47{\mu}g/m{\ell}$], nitric oxide (NO) production inhibitory effect on the mouse macrophage cell line RAW264.7 [inhibitory concentration 50% $(IC_{50})=60.9{\pm}7.52{\mu}g/m{\ell}$] and cytotoxicity effect on the human hepatoma cell line, HepG2 [lethal concentration 50% $(LC_{50})=115.4{\pm}1.86{\mu}g/m{\ell}$]. According to the bioassay-based method, two cytotoxic compounds were purified from the $Et_2O$ fraction by using column chromatography and preparative high performance liquid chromatography (prep-HPLC). These two compounds were identified as parietin and chrysophanol by using nuclear magnetic resonance (NMR) and liquid chromatography quadruple time of flight mass spectrometry (LC-QTOF-MS). In addition, both parietin and chrysophanol exhibited a cytotoxicity effect on HepG2 cells, their $LC_{50}$ values were $169.1{\pm}17.67{\mu}M$ and $111.5{\pm}6.62{\mu}M$, respectively. Conclusions: Parietin and chrysophanol isolated from the $Et_2O$ fraction of the R. crispus root extract showed cytotoxicity in HepG2 cell.

• 한국환경과학회지
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• 제21권10호
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• pp.1265-1274
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• 2012

This study was conducted to investigate the extraction characteristics including total polyphenol compound content (TPC) and the antioxidative effect of Rumex crispus root extract on commercial corn oil. Extraction yield was increased with extraction temperature but decreased with extraction ratio. No significant differences in aromatics content were found among the extracts prepared with various extraction conditions; extraction ratio, extraction temperature, extraction pH and composition of extractant. Total flavonoid content of the extract was increased with extraction temperature and extraction pH, and highest value of it was found when extractant composition of ethanol in water was 75%. Total polyphenol compound content (TPC) of the extract with 10 fold extraction ratio showed the highest value, but no significant difference in TPC was found with extraction temperature. Reducing power and DPPH (${\alpha}$,${\alpha}$-diphenyl-${\beta}$-picrylhydrazyl) radical scavenging ability (RSA) of the extract in spectrophotometric absorbance were decreased with extraction ratio but increased with extraction temperature and showed 63.1%~98.4% and 67.6%~86.7% of those of ascorbic acid in reducing power and DPPH RSA, respectively. The antioxidative effects of the extract on corn oil were 84.8~93.0% of that of commercial butylated hydroxytoluene, the antioxidant index value was highest when the ethanol composition to water in extractant was equal ratio.

• The Korean Journal of Ecology
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• 제15권2호
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• pp.137-145
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• 1992

When exposed to 1, 700 ml of 0.089, 0.445 or 0.890 mM/l cadmius solution, dock (rumex crispus L)plants from heavily polluted chungrangchon site absorbed 0.0404, 0.2244 or 0.4929 mM of cadmium per g dw during the first 4 hours, which were 5.0~30.8 times faster uptake rate than those of water hyacinth (eichhornia crassipes Solm. - laub). Zinc, with which cadmium generally occurs together, did not affect the uptake rate in the concentration range of 0.0306 ~0.0918 mM/l. rumex cripus from chungrangchon site and from less polluted shingalchon site, when exposed to 1, 700 ml of 0.089 mM/l solution for 3 days, removed 68.4% and 63.2%, repectively, of the cadmium initially present in the solution. And when exposed to cadmium solution of the same concentration for the second time after cadmium-free hydroponic culture for three days, these plants removed 76.1% and 66.8% of cadmium, respectively. These removal rates were not significantly different between collection sites or between exposures, but were about 2 times greater for the first exposure, and 2~5 times greater for the second exposure, than those of water hyacinth. these results incate that rumex cripus can absorb, and thus remove, large amount of cadmium ion in wastewater, and so can be used in wastewater treatment, e.g. soil trench method. since there was large difference among the plants in the ability to absorb cadmium, it should be possible to select for strain with greater ability.

• 한국잡초학회지
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• 제30권3호
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• pp.183-190
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• 2010

본 연구의 목적은 국내 자생식물인 소리쟁이 (Rumex crispus)로부터 살초활성물질을 분리하고, 그 화학구조를 구명하는데 있다. 소리쟁이 메탄올 추출물의 유채에 대한 $GR_{50}$값은 $935{\mu}g\;g^{-1}$이었다. 메탄올 추출물을 에틸아세테이트로 분획한 분획층을 대상으로 activity-directed bioassay를 수행하여 가장 살초활성이 높았던 ECDA 분획물을 얻었다($GR_{50}$ 값, $53{\mu}g\;g^{-1}$). ECDA 분획물을 GC-MS, $^1H$-NMR, $^{13}C$-NMR로 분석한 결과 분자량 186.2, 화학구조식 $C_{11}H_6O_3$, furanocoumarin 골격구조를 하고 있는 2Hfuro[2,3-H]-[1]-benzopyran-2-one(angelicin)으로 밝혀졌다. Angelicin의 물피(Echinochloa crus-galli), 바랭이(Digitaria ciliaris), 자귀풀(Aeschynomene indica)에 대한 seed bioassay 결과 각 식물에 대한 $GR_{50}$값은 426, 66, $216{\mu}g\;g^{-1}$이었다. 본 연구의 결과는 소리쟁이 유래 신규 살초활성물질 angelicin은 향후 새로운 제초제 개발을 위한 선도물질로 이용될 수 있을 것이라 판단된다.

• 약학회지
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• 제58권5호
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• pp.307-313
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• 2014

Rumex crispus (curled dock), which is a perennial wild plant, has long been used as a laxative, astringent, and medicine to treat blood and skin diseases. We recently reported that the roots of R. crispus are an effective nutraceutical for bone. This study prepared ethanol extracts of the leaves and roots of R. crispus, and analyzed the major constituents using liquid chromatography and mass spectrometry. In addition, their effects on the proliferation and differentiation of human osteoblast-like MG-63 cells, such as cell viability, alkaline phosphatase (ALP) activity, collagen content, and mineralization, were compared. The chromatograms of the chemical constituents of the two extracts exhibited quite different profiles: quercetin and quercitrin were identified as major peaks in the leaf extract, whereas cinnamtannin B1 and procyanidin isomers were the major peaks for the root extract. Neither extract was cytotoxic at concentrations of < $25{\mu}g/ml$. ALP activity and collagen synthesis-which are markers of the early stage of osteogenesis-in MG-63 cells were significantly increased upon the addition of the root extract compared with the addition of the leaf extract. In contrast, the leaf extract had a more stimulatory effect on mineralization-which is marker of the late stage of osteogenesis-in MG-63 cells than did the root extract. In conclusion, extracts of both leaves and roots of R. crispus stimulated the bone-forming activity of osteoblasts; in particular, the root extract was more effective in the early stage of osteoblast differentiation, while the leaf extract was more effective in the late stage. This difference in anabolic activity may be due to differences in the constituents of the leaves and roots. The leaves and roots of R. crispus appear to complement each other as stimulators of bone formation.

• 아시안잔디학회지
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• 제24권1호
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• pp.31-35
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• 2010

온도조건과 광의 유무에 의한 소리쟁이속 잡초종자의 발아특성을 조사한 결과, 발아 시작점은 참소리쟁이가 치상 4일 후로 가장 빨랐으며 돌소리쟁이(5일), 소리쟁이(6일), 좀소리쟁이(10일)의 순으로 나타났다. 소리쟁이속 잡초의 최적 발아온도는 공시 잡초종자 모두 $15^{\circ}C$에서 가장 높은 발아율을 보였으며, 돌소리쟁이가 $10^{\circ}C{\sim}30^{\circ}C$까지 높은 발아율을 보여 온도에 있어 가장 넓은 발아스펙트럼을 가지고 있는 것으로 나타났다. 1996년 수집하여 저온저장한 참소리쟁이와 2004년 수집된 참소리쟁이 간의 종자생존력을 비교한 결과, 두 초종간의 유의차는 인정되지 않아 참소리쟁이는 저온저장 할 경우 8년 이상의 종자생존력을 가지고 있는 것으로 나타났다. 조사기간 중 암조건에서 수행한 모든 처리에서 발아는 보이지 않았다. 소리쟁이속 잡초의 발아특성을 수량화한 결과, 참소리쟁이는 다른 소리쟁이속 잡초에 비해 가장 높은 누적발아율을 보이며 발아에 균일성을 보였다. 돌소리쟁이는 참소리쟁이나 소리쟁이에 비해 발아에 걸리는 평균일수나 발아속도가 가장 빠른 것으로 나타났다.