• Asian-Australasian Journal of Animal Sciences
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• v.8 no.1
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• pp.37-41
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• 1995

Streptococci and enterococci, isolates from the rumen content of mouflons and European bisons were isolated. The total counts of these species reached the values(log 10 ${\pm}$ S.E.M.) $7.3{\pm}0.21$; $6.1{\pm}0.06$ bacteria per one ml of the rumen content in streptococci and $3.6{\pm}0.20$; $3.17{\pm}0.18$ bacteria per one ml of the rumen content in enterococci, Strains isolated were allotted to te species Streptococcus bovis(AM1, AM2, AM3, AM4), Enterococcus faecium(EH1, EFG2, EC3) and Enterococcus faecalis (EFA1, EFD2). Bactera presented belong to the strains with low urease and ${\alpha}$-amylase activities. The majority of isolates were polyresistant. Each strain produced bacteriocin - like substance with effect against at least of one of relatives species as indicators used. The most of inhibition zones were hazy with the width 2-6 mm in diameter.

• Asian-Australasian Journal of Animal Sciences
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• v.2 no.3
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• pp.441-443
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• 1989

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.8
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• pp.1277-1284
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• 1999

Eight feeds and their residues left after washing with tap water (water residue) or incubation in the rumen (rumen residues) were treated with hydrochloric acid, neutral detergent solution without EDTA (NDS) or both, and the release or sorption of minerals (Ca, Mg, P, Na, K, Cu and Zn) assessed. Six of the feeds were from Sri Lanka (Panicum maximum ecotype Guinea A, Glyricidia maculate, Artocarpus heterophyllus (jak leaves), untreated and urea-treated rice straw, and rice bran) and two from the Netherlands (maize silage and wheat straw). The initial concentration of mineral elements, the concentration of neutral detergent fibre (NDF) and the type of feed significantly influenced (p<0.01). The proportion of the mineral elements released or sorbed. In general, feeds with high NDF content (straws and guinea grass) sorbed Ca from tap water, or released less in the rumen, and within these feeds the extent of sorption varied with source of fibre. Acid or NDS treatment removed little of the sorbed Ca, but they removed much of the Mg from both water and rumen residues. Fibres of wheat straw and jak leaves showed an affinity for Mg in the rumen. All feeds and their water and rumen residues sorbed P and Na from NDS, and the extent of sorption varied with the initial concentrations of these elements and with the type of fibre. Acid treatment removed part of the sorbed Na, but not the P. The solubility of K was not affected by the content of NDF, the type of fibre or the initial concentration of K. All feeds and their residues, except for the rumen residues of rice bran sorbed Cu from tap water and in the rumen. The recovery of Cu in rumen residues declined from 353% to 147% after NDS treatment, and with some feeds (glyricidia and jak leaves) the recovery was below 100%. Acid treatment removed part of the Zn sorbed by the water and rumen residues, but the capacity of residues to retain Zn varied with the type of feed.

• Asian-Australasian Journal of Animal Sciences
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• v.4 no.2
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• pp.161-164
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• 1991

A rapid and accurate method is described for the determination of nucleo-bases in rumen micro-organisms. A procedure to satisfactorily hydrolyse the micro-organisms involving reaction with a mixture of readily volatile organic acids (acetic and formic acids) under high pressure, is proposed, and optimal conditions for an analytical procedure with reversed phase HPLC is described. The following nucleobases contents (mmol/kg DM) of rumen micro-organisms were found: Adenine (Ade), 82.62; Guanine (Gua), 61.34; Cytosine (Cyt), 84.61; Thymine (Thy), 35.74; Uracil (Ura), 68.62; Hypoxanthine (Hxn), 13.06; Xanthine (Xn), 8.35. Total purine-N content (g/kg N) of rumen micro-organisms were 99.60. The nucleic acid N content (g/kg N) of microbial isolates were: RNA-N, 109.9; DNA-N, 50.9.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.7
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• pp.1003-1010
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• 2003

The effects of different rumen protected forms, oleamide, Ca oleate, of dietary oleic acid on the carcass quality and fatty acid composition in intramuscular and subcutaneous fat tissues of Hanwoo steer were examined. Sixty, 25 month old Hanwoo steers divided into three groups were fed no supplement (Control), 2% of oleamide (Oleamide) or Ca-oleate (Ca-Oleate) in their diet for 45 or 90 days. Disappearance rates of oleic acid supplements in digestive tracts (Rumen bypass, abomasal and intestinal disappearance rate) were 48.5, 68.4 for oleamide and Ca oleate, respectively. Both oleic acid supplements affected feed intake, growth rate, cold carcass weight and carcass fatness. Live weight gain, carcass weight, backfat thickness and marbling score were higher in the oleic acid supplemented steers compared with those from the control. Oleic acid supplements increased marbling score and ether extract in Hanwoo steer m. logissi thoracicmus. Rumen protected oleic acid increased not only the level of oleic acid but also polyunsaturated fatty acids in intramuscular and subcutaneous fat tissue. Total saturated fatty acid contents in both fat tissues were decreased whereas total unsaturated fatty acid content was increased compared with those from control. Linoleic acid, linolenic acid and polyunsaturated fatty acid contents were significantly higher in Ca oleate than any other steers. Lipid metabolites in blood were increased in rumen protected oleic acid treatments. HDL content in blood was increased in Ca-oleate supplemented steers whereas LDL was decreased compared with control. The changes of fatty acid compositions in the rumen protected oleic acid supplemented steers suggest that the oleic acid and unsaturated fatty acid were protected from rumen biohydrogenation and can be deposited in the fat tissues.

• Animal Bioscience
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• v.35 no.10
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• pp.1535-1544
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• 2022

Objective: The objective of this study was to investigate the effects of decreasing dietary crude protein content on rumen fermentation, mictobiota, and metabolites in goats. Methods: In an 84-day feeding trial, a total of twelve male Anhui white goat kids with initial body weight 15.9±1.13 kg were selected and randomly classified into two groups, feeding a normal crude protein diet (14.8% CP, NCP) or a low crude protein diet (12.0% CP, LCP). At the end of the experimental trial (on day 84), six animals were randomly selected from each group and were slaughtered to collect rumen fluid samples for the analysis of rumen fermentation parameters, microbiome, and metabolome. Results: The concentrations of ammonia-nitrogen, total volatile fatty acid, acetate, and propionate were decreased (p<0.05) in the LCP group in comparison with those in the NCP group. The abundances of genera Prevotella, Campylobacter, Synergistetes, and TG5, which were associated with nitrogen metabolism, were lower (p<0.05) in the LCP group compared with those in the NCP group. The levels of 78 metabolites (74 decreased, 4 increased) in the rumen fluid were altered (p<0.05) by the treatment. Most of the ruminal metabolites that showed decreased levels in the LCP group were substrates for microbial protein synthesis. Metabolic pathway analysis showed that vitamin B6 metabolism was significantly different (p<0.05) in rumen fluid between the two treatments. Conclusion: Decreased dietary protein level inhibited rumen fermentation through microbiome and metabolome shifts in goat kids. These results enhance our understanding of ruminal bacteria and metabolites of goat fed a low protein diet.

• Animal Bioscience
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• v.34 no.11
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• pp.1784-1793
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• 2021

Objective: An experiment was conducted to evaluate the effects of bamboo leaf extract (BLE) on the production performance, rumen fermentation parameters, and rumen bacterial communities of heat-stressed dairy cows. Methods: The experiment comprised a 14-day adaptation period and a 21-day experimental period and was conducted in a high-temperature and humidity environment (daily mean ambient temperature = 33.5℃±1.3℃; daily mean relative humidity = 64.9%±0.8%, daily mean temperature-humidity index = 86.2±0.4). Twelve Holstein dairy cows were randomly allocated into two groups. A total mixed ration supplemented with BLE at 0 (CON) and 1.3 g/kg dry matter (DM) were fed, respectively. Feed intake and milk yield were recorded daily. Milk samples were collected on 1, 11, and 21 d of the experimental period to analyze milk performance. Rumen fluid samples were collected on 21 d of the experimental period to analyze rumen fermentation parameters and rumen bacterial communities. Results: Compared with the control group, supplementation of BLE increased milk yield (p<0.01), milk fat yield (p = 0.04), 4% fat-corrected milk (p<0.01) and milk fat content (p<0.01); reduced somatic cell count (p<0.01). No differences in DM intake and milk protein or lactose content were observed between two groups. Supplementation of BLE also increased the rumen total volatile fatty acid (p<0.01), acetate (p<0.01), butyrate (p<0.01), and valerate (p = 0.05) concentrations. However, no significant effects were observed on rumen pH, ammonia nitrogen, propionate, acetate/propionate ratio, isobutyrate, or isovalerate. Furthermore, BLE increased the rumen bacterial abundance and the diversity of the rumen bacterial community. The BLE reduced the Firmicutes/Bacteroidetes abundance ratio and increased the abundances of Butyrivibrio_2 (p<0.01) and Ruminococcus_2 (p<0.01). Conclusion: The BLE supplementation at 1.3 g/kg DM could improve production performance and rumen fermentation in dairy cows during heat stress.

• Asian-Australasian Journal of Animal Sciences
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• v.7 no.1
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• pp.41-48
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• 1994

This experiment was carried out in order to evaluate the chemical composition and rumen degradation characteristics of Italian ryegrass harvested at three different growth stages, i,e. pre-blooming, early-blooming and late-blooming. Degradation values were obtained by incubation of the samples using the nylon bag technique on the rumen of sheep fed a normal diet (Timothy hay with 200 g/d concentrate per head) for 12, 24, 36, 48 and 72 hours, respectively. Neutral detergent fiver (NDF) content was highest at late-blooming (64.4%) while no difference was found among the pre-blooming and early-blooming (49.4% vs 48.3%). However, acid detergent fiber (ADF) content markedly increased from 30.0% at pre-blooming to 35.4% and 46.4% at early-blooming and late-blooming, respectively. Lignin and silica contents also increased as advancing maturity of the grass, Rumen degradation of dry matter (DM) significantly reduced (p < 0.05) as advancing maturity of the grass. Ruman degradation cellulose and ADF at pre-blooming were significantly higher (p < 0.05) than those of early-blooming and late-blooming. However, no significant differences were observed among the early-blooming and late-blooming. With advancing maturity, rumen degradation of NDF and hemicellulose significantly reduced (p < 0.05) at the incubation times.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.9
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• pp.1280-1284
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• 2003

The number of protozoa and VFA content in the rumen fluid, in situ disappearance and turnover rate were examined with four rumen-fistulated cattle given either sugarcane stalk or Ruzi grass hay in order to clarify the manner of rumen digestion of sugarcane stalk. Cattle were given either sugarcane stalk or Ruzi grass hay at 1.0% of body weight level with commercial concentrate feed. Feeding sugarcane stalk reduced acetate content and increased propionate and butyrate contents in rumen fluid. While rapidlysoluble fraction of sugarcane stalk was 42%, the insoluble but potentially degradable fraction was only 17%. This clearly showed that sugarcane stalk mainly consisted of water soluble fraction (i.e. sugar) and tough fiber (i.e. bagasse). The ruminal degradation rate of both Ruzi grass hay and sugarcane stalk was lower in the animal given sugarcane stalk in comparison with those given Ruzi grass hay. While the turnover rate of liquid phase was about 50% higher in the animals given sugarcane stalk than in the animals given Ruzi grass hay, that of the solid phase was about 40% lower in the animals given sugarcane stalk. The effective degradability of DM of sugarcane stalk was higher than that of Ruzi grass hay. Sugarcane would be a promising roughage for ruminants in the tropics especially, in the dry season.

• Membrane and Water Treatment
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• v.10 no.3
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• pp.213-221
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• 2019

Anaerobic digestion (AD) has been widely used to valorize food waste (FW) because of its ability to convert organic carbon into $CH_4$ and $CO_2$. Korean FW has a high content of fruits and vegetables, and efficient hydrolysis of less biodegradable fibers is critical for its complete stabilization by AD. This study examined the digestates from different anaerobic digesters, namely Rs, Rr, and Rm, as the inocula for the AD of vegetable waste (VW) and cellulose (CL): Rs inoculated with anaerobic sludge from an AD plant, Rr inoculated with rumen fluid, and Rm inoculated with anaerobic sludge and augmented with rumen fluid. A total of six conditions ($3\;inocula{\times}2\;substrates$) were tested in serial subcultures. Biogas yield was higher in the runs inoculated with Rm than in the other runs for both VW (up to 1.10 L/g VS added) and CL (up to 1.05 L/g VS added), and so was biogas production rate. The inocula had different microbial community structures, and both substrate type and inoculum source had a significant effect on the formation and development of microbial community structures in the subcultures. The overall results suggest that the bioaugmentation with rumen microbial consortium has good potential to enhance the anaerobic biodegradability of VW, and thereby can help more efficiently digest high fiber-content Korean FW.