Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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DETERMINATION OF PURINE AND PYRIMIDINE BASES IN RUMEN MICRO-ORGANISMS BY REVERSED PHASE HPLC AFTER HYDROLYTIC DIGESTION UNDER PRESSURE

  • Han, Y.K. (Department of Animal Science, College of Agriculture, Seoul National Univ.) ;
  • Landis, J. (Gruppe Ernahrung, Institut fur Nutztierwissenschaften)
  • Received : 1990.06.20
  • Accepted : 1991.03.04
  • Published : 1991.06.01
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Abstract

A rapid and accurate method is described for the determination of nucleo-bases in rumen micro-organisms. A procedure to satisfactorily hydrolyse the micro-organisms involving reaction with a mixture of readily volatile organic acids (acetic and formic acids) under high pressure, is proposed, and optimal conditions for an analytical procedure with reversed phase HPLC is described. The following nucleobases contents (mmol/kg DM) of rumen micro-organisms were found: Adenine (Ade), 82.62; Guanine (Gua), 61.34; Cytosine (Cyt), 84.61; Thymine (Thy), 35.74; Uracil (Ura), 68.62; Hypoxanthine (Hxn), 13.06; Xanthine (Xn), 8.35. Total purine-N content (g/kg N) of rumen micro-organisms were 99.60. The nucleic acid N content (g/kg N) of microbial isolates were: RNA-N, 109.9; DNA-N, 50.9.

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