Membrane and Water Treatment

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Enhancing anaerobic digestion of vegetable waste and cellulose by bioaugmentation with rumen culture

  • Jo, Yeadam (School of Urban and Environmental Engineering, Ulsan National Institute of Science and Technology (UNIST)) ;
  • Hwang, Kwanghyun (Environmental Process Engineering Team, Global Engineering Division, GRAN SEOUL) ;
  • Lee, Changsoo (School of Urban and Environmental Engineering, Ulsan National Institute of Science and Technology (UNIST))
  • Received : 2018.10.01
  • Accepted : 2018.12.15
  • Published : 2019.05.25
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Abstract

Anaerobic digestion (AD) has been widely used to valorize food waste (FW) because of its ability to convert organic carbon into $CH_4$ and $CO_2$. Korean FW has a high content of fruits and vegetables, and efficient hydrolysis of less biodegradable fibers is critical for its complete stabilization by AD. This study examined the digestates from different anaerobic digesters, namely Rs, Rr, and Rm, as the inocula for the AD of vegetable waste (VW) and cellulose (CL): Rs inoculated with anaerobic sludge from an AD plant, Rr inoculated with rumen fluid, and Rm inoculated with anaerobic sludge and augmented with rumen fluid. A total of six conditions ($3\;inocula{\times}2\;substrates$) were tested in serial subcultures. Biogas yield was higher in the runs inoculated with Rm than in the other runs for both VW (up to 1.10 L/g VS added) and CL (up to 1.05 L/g VS added), and so was biogas production rate. The inocula had different microbial community structures, and both substrate type and inoculum source had a significant effect on the formation and development of microbial community structures in the subcultures. The overall results suggest that the bioaugmentation with rumen microbial consortium has good potential to enhance the anaerobic biodegradability of VW, and thereby can help more efficiently digest high fiber-content Korean FW.

Keywords

Acknowledgement

Grant : Human Resources Program in Energy Technology

Supported by : Korea Institute of Energy Technology Evaluation and Planning (KETEP), National Research Foundation of Korea (NRF)

References

  1. Ahring, B.K. (2003), Biomethanation I, 81, Springer, New York, U.S.A.
  2. APHA-AWWA-WEF. (2005), Standard Methods for the Examination of Water and Wastewater, American Public Health Association, Washington, D.C., U.S.A.
  3. Aragaw, T., Andargie, M. and Gessesse, A. (2013), "Co-digestion of cattle manure with organic kitchen waste to increase biogas production using rumen fluid as inoculums", Int. J. Phys. Sci., 8, 443-450.
  4. Baba, Y., Tada, C., Fukuda, Y. and Nakai, Y. (2013), "Improvement of methane production from waste paper by pretreatment with rumen fluid", Bioresour. Technol., 128, 94-99. https://doi.org/10.1016/j.biortech.2012.09.077
  5. Bialek, K., Kim, J., Lee, C., Collins, G., Mahony, T. and O'Flaherty, V. (2011), "Quantitative and qualitative analyses of methanogenic community development in high-rate anaerobic bioreactors", Water Res., 45, 1298-1308. https://doi.org/10.1016/j.watres.2010.10.010
  6. Biddle, A., Stewart, L., Blanchard, J. and Leschine, S. (2013), "Untangling the genetic basis of fibrolytic specialization by Lachnospiraceae and Ruminococcaceae in diverse gut communities", Diversity., 5, 627-640. https://doi.org/10.3390/d5030627
  7. Bouallagui, H., Ben Cheikh, R., Marouani, L. and Hamdi, M. (2003), "Mesophilic biogas production from fruit and vegetable waste in a tubular digester", Bioresour. Technol., 86, 85-89. https://doi.org/10.1016/S0960-8524(02)00097-4
  8. Bouallagui, H., Touhami, Y., Ben Cheikh, R. and Hamdi, M. (2005), "Bioreactor performance in anaerobic digestion of fruit and vegetable wastes", Process Biochem., 40, 989-995. https://doi.org/10.1016/j.procbio.2004.03.007
  9. Chassard, C., Delmas, E., Robert, C. and Bernalier-Donadille, A. (2010), "The cellulose-degrading microbial community of the human gut varies according to the presence or absence of methanogens", FEMS Microbiol. Ecol., 74, 205-213. https://doi.org/10.1111/j.1574-6941.2010.00941.x
  10. Divya, D., Gopinath, L. and Christy, P.M. (2015), "A review on current aspects and diverse prospects for enhancing biogas production in sustainable means", Renew. Sust. Energy Rev., 42, 690-699. https://doi.org/10.1016/j.rser.2014.10.055
  11. Duskova, D. and Marounek, M. (2001), "Fermentation of pectin and glucose, and activity of pectin-degrading enzymes in the rumen bacterium Lachnospira multiparus", Lett. Appl. Microbiol., 33, 159-163. https://doi.org/10.1046/j.1472-765x.2001.00970.x
  12. Garcia-Pena, E., Parameswaran, P., Kang, D., Canul-Chan, M. and Krajmalnik-Brown, R. (2011), "Anaerobic digestion and codigestion processes of vegetable and fruit residues: Process and microbial ecology", Bioresour. Technol., 102, 9447-9455. https://doi.org/10.1016/j.biortech.2011.07.068
  13. Ge, X., Xu, F. and Li, Y. (2016), "Solid-state anaerobic digestion of lignocellulosic biomass: recent progress and perspectives", Bioresour. Technol., 205, 239-249. https://doi.org/10.1016/j.biortech.2016.01.050
  14. Grabowski, A., Tindall, B.J., Bardin, V., Blanchet, D. and Jeanthon, C. (2005), "Petrimonas sulfuriphila gen. nov., sp. nov., a mesophilic fermentative bacterium isolated from a biodegraded oil reservoir", Int. J. Syst. EMicrobiol., 55, 1113-1121. https://doi.org/10.1099/ijs.0.63426-0
  15. Hatamoto, M., Kaneshige, M., Nakamura, A. and Yamaguchi, T. (2014), "Bacteroides luti sp. nov., an anaerobic, cellulolytic and xylanolytic bacterium isolated from methanogenic sludge", Int. J. Syst. Evol. Microbiol., 64, 1770-1774. https://doi.org/10.1099/ijs.0.056630-0
  16. Hu, Z.H. and Yu, H.Q. (2005), "Application of rumen microorganisms for enhanced anaerobic fermentation of corn stover", Process Biochem., 40, 2371-2377. https://doi.org/10.1016/j.procbio.2004.09.021
  17. Hwang, S., Kim, J. and Sung, C. (1996), "Analysis of dietary fiber content of some vegetables, mushrooms, fruits and seaweeds", Korean. J. Nutr., 29, 89-96.
  18. Imachi, H., Sakai, S., Sekiguchi, Y., Hanada, S., Kamagata, Y., Ohashi, A. and Harada, H. (2008), "Methanolinea tarda gen. nov., sp. nov., a methane-producing archaeon isolated from a methanogenic digester sludge", Int. J. Syst. Evol. Microbiol., 58, 294-301. https://doi.org/10.1099/ijs.0.65394-0
  19. Jin, W., Xu, X. and Yang, F. (2018), "Application of rumen microorganisms for enhancing biogas production of corn straw and livestock manure in a pilot-scale anaerobic digestion system: performance and microbial community analysis", Energies., 11, 920. https://doi.org/10.3390/en11040920
  20. Jo, Y., Kim, J., Park, T., Hwang, K., Kim, D. and Lee, C. (Accepted), "Potential for enhanced biomethanation for food waste by bioaugmentation with rumen fluid", Desalin. Water Treat. In Press.
  21. Kafle, G.K., Bhattarai, S., Kim, S.H. and Chen, L. (2014), "Effect of feed to microbe ratios on anaerobic digestion of Chinese cabbage waste under mesophilic and thermophilic conditions: biogas potential and kinetic study", J. Environ. Manage., 133, 293-301. https://doi.org/10.1016/j.jenvman.2013.12.006
  22. Kafle, G.K. and Kim, S.H. (2013), "Anaerobic treatment of apple waste with swine manure for biogas production: batch and continuous operation", Appl. Energy., 103, 61-72. https://doi.org/10.1016/j.apenergy.2012.10.018
  23. Kim, J. and Lee, C. (2015), "Response of a continuous biomethanation process to transient organic shock loads under controlled and uncontrolled pH conditions", Water Res., 73, 68-77. https://doi.org/10.1016/j.watres.2015.01.015
  24. Kim, J., Lee, S. and Lee, C. (2013), "Comparative study of changes in reaction profile and microbial community structure in two anaerobic repeated-batch reactors started up with different seed sludges", Bioresour. Technol., 129, 495-505. https://doi.org/10.1016/j.biortech.2012.11.119
  25. Lee, C., Kim, J., Hwang, K., O'Flaherty, V. and Hwang, S. (2009), "Quantitative analysis of methanogenic community dynamics in three anaerobic batch digesters treating different wastewaters", Water Res., 43, 157-165. https://doi.org/10.1016/j.watres.2008.09.032
  26. Lee, C., Kim, J., Shin, S.G., O'Flaherty, V. and Hwang, S. (2010), "Quantitative and qualitative transitions of methanogen community structure during the batch anaerobic digestion of cheese-processing wastewater", Appl. Microbiol. Biotechnol., 87, 1963-1973. https://doi.org/10.1007/s00253-010-2685-1
  27. Lee, K.S. and Lee, S.R. (1993), "Analysis of dietary fiber content in Korean vegetable foods", Korean J. Food Sci. Technol., 25, 225-231.
  28. Li, C., Champagne, P. and Anderson, B.C. (2011), "Evaluating and modeling biogas production from municipal fat, oil, and grease and synthetic kitchen waste in anaerobic co-digestions", Bioresour. Technol., 102, 9471-9480. https://doi.org/10.1016/j.biortech.2011.07.103
  29. Lim, J.W. and Wang, J.Y. (2013), "Enhanced hydrolysis and methane yield by applying microaeration pretreatment to the anaerobic co-digestion of brown water and food waste", Waste Manage., 33, 813-819. https://doi.org/10.1016/j.wasman.2012.11.013
  30. Lu, F., Hao, L., Zhu, M., Shao, L. and He, P. (2012), "Initiating methanogenesis of vegetable waste at low inoculum-to-substrate ratio: importance of spatial separation", Bioresour. Technol., 105, 169-173. https://doi.org/10.1016/j.biortech.2011.11.104
  31. Mao, S., Zhang, R., Wang, D. and Zhu, W. (2013), "Impact of subacute ruminal acidosis (SARA) adaptation on rumen microbiota in dairy cattle using pyrosequencing", Anaerobe., 24, 12-19. https://doi.org/10.1016/j.anaerobe.2013.08.003
  32. Marin, J., Kennedy, K.J. and Eskicioglu, C. (2010), "Effect of microwave irradiation on anaerobic degradability of model kitchen waste", Waste Manage., 30, 1772-1779. https://doi.org/10.1016/j.wasman.2010.01.033
  33. McCune, B., Grace, J.B. and Urban, D.L. (2002), Analysis of ecological communities, 28, MjM software design Gleneden Beach, OR.
  34. Moon, H.C. and Song, I. (2011), "Enzymatic hydrolysis of foodwaste and methane production using UASB bioreactor", Int. J. Green Energy., 8, 361-371. https://doi.org/10.1080/15435075.2011.557845
  35. Ray, M.J., Leak, D.J., Spanu, P.D. and Murphy, R.J. (2010), "Brown rot fungal early stage decay mechanism as a biological pretreatment for softwood biomass in biofuel production", Biomass. Bioenergy., 34, 1257-1262. https://doi.org/10.1016/j.biombioe.2010.03.015
  36. Sharma, P. and Melkania, U. (2018), "Effect of bioaugmentation on hydrogen production from organic fraction of municipal solid waste", Int. J. Hydrogen. Energy., 43, 7290-7298. https://doi.org/10.1016/j.ijhydene.2018.03.031
  37. Shin, S.G., Han, G., Lim, J., Lee, C. and Hwang, S. (2010), "A comprehensive microbial insight into two-stage anaerobic digestion of food waste-recycling wastewater", Water Res., 44, 4838-4849. https://doi.org/10.1016/j.watres.2010.07.019
  38. Vavouraki, A.I., Angelis, E.M. and Kornaros, M. (2013), "Optimization of thermo-chemical hydrolysis of kitchen wastes", Waste Manage., 33, 740-745. https://doi.org/10.1016/j.wasman.2012.07.012
  39. Wang, J.Y., Liu, X.Y., Kao, J. and Stabnikova, O. (2006), "Digestion of pre-treated food waste in a hybrid anaerobic solid-liquid (HASL) system", J. Chem. Technol. Biotechnol., 81, 345-351. https://doi.org/10.1002/jctb.1401
  40. Wang, L., Shen, F., Yuan, H., Zou, D., Liu, Y., Zhu, B. and Li, X. (2014), "Anaerobic co-digestion of kitchen waste and fruit/vegetable waste: Lab-scale and pilot-scale studies", Waste Manage., 34, 2627-2633. https://doi.org/10.1016/j.wasman.2014.08.005
  41. Zhang, H., Zhang, P., Ye, J., Wu, Y., Fang, W., Gou, X. and Zeng, G. (2016), "Improvement of methane production from rice straw with rumen fluid pretreatment: A feasibility study", Int. Biodeterior. Biodegrad., 113, 9-16. https://doi.org/10.1016/j.ibiod.2016.03.022
  42. Zhao, B.H., Yue, Z.B., Ni, B.J., Mu, Y., Yu, H.Q. and Harada, H. (2009), "Modeling anaerobic digestion of aquatic plants by rumen cultures: cattail as an example", Water Res., 43, 2047-2055. https://doi.org/10.1016/j.watres.2009.02.006
  43. Zhou, M. and Hernandez-Sanabria, E. (2009), "Assessment of the microbial ecology of ruminal methanogens in cattle with different feed efficiencies", Appl. Environ. Microbiol., 75, 6524-6533. https://doi.org/10.1128/AEM.02815-08
  44. Zumstein, E., Moletta, R. and Godon, J.J. (2000), "Examination of two years of community dynamics in an anaerobic bioreactor using fluorescence polymerase chain reaction (PCR) singlestrand conformation polymorphism analysis", Environ. Microbiol., 2, 69-78. https://doi.org/10.1046/j.1462-2920.2000.00072.x

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