• Journal of Physiology & Pathology in Korean Medicine
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• v.31 no.3
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• pp.159-166
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• 2017

Through this study, the authors investigated the anti-steatosis effects of the Amomum cardamomum ethyl acetate fraction in free fatty acids (FFAs)-induced human hepatocellular carcinoma HepG2 cells. The ethyl acetate fraction of Amomum cardamomum (ACEA) was extracted with 70% ethanol and then the extract was evaporated using a rotary evaporator prior to sequential fractionation. Human hepatocellular carcinoma were treated with different concentrations of ACEA in the presence and absence of FFAs. To demonstrate the reactive oxygen species (ROS) scavenging activity, DCFDA level was analyzed by using in vitro assay system. Cell viability, lipid accumulation, intracellular triglycerides, malondialdehyde (MDA), liver steatosis related signaling molecules and inflammatory cytokines such as interleukin (IL)-6, 8, tumor necrosis factor-alpha ($TNF-{\alpha}$) were also investigated. As results, ACEA inhibited the FFAs-induced ROS, lipid accumulation, intracellular triglycerides, and MDA in a dose dependent manner. Treatment of human hepatocellular cells with ACEA induced the phosphorylation of 5' adenosine monophosphate-activated protein kinase (AMPK) and carnitine palmitoyltransferase I (CPT1) expression using western blot analysis. ACEA also potently suppressed the FFAs-induced inflammatory cytokines including IL-6, IL-8 and $TNF-{\alpha}$. These results suggest that the ethyl acetate fraction of Amomum cardamoum extract own inhibitory effects of liver steatosis by inhibiting ROS, lipid accumulation, intracellular triglycerides, MDA through AMPK signaling and anti-inflammatory actions.

• Journal of computational fluids engineering
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• v.20 no.4
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• pp.81-92
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• 2015

Construction waste mainly consists of concrete aggregates of various size. Improper handling of concrete waste would be a major environmental problem whereas its recycling would be both economically useful and environmentally friendly. Bigger concrete aggregates are crushed and converted to medium and fine particles to make them recyclable. An apparatus to separate the concrete aggregates by their size is thus needed for their effective recycling. In this work, segregation of concrete particles in air flows from a newly designed rotary separator having three stages of blades is simulated using a commercial software, ANSYS-CFX. Both 2-D and 3-D models with 360, 240 and 180 blades in each stage are considered. Fundamental mechanism of separation of particles(pase) and the effect of design parameters such as particle size, rotor speed, air flow rate etc. on the performance of the separator are investigated. Critical size of particles that can be separated by the developed separator is also presented in this work. Simulation results are overall in good agreement with data predicted from the theoretical model previously reported in the companion paper.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.43 no.8
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• pp.712-721
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• 2015

Pulse Detonation Engine (PDE) has been considered as a future propulsion system for broad range of operation and higher thermal efficiency. Various subsystem technologies have been studied for more than decade to improve the performance of the potential system. New valve systems has been developed for the stable operation at high frequency including inflow-driven valve, rotary valve and valveless system. To foster the detonation initiation with a little ignition energy, plasma ignition method and DDT (deflagration to detonation transition) acceleration method such as swept ramp mechanism have been studied. Fluidic nozzle system and other nozzle system are the ongoing research topics to maximize the propulsion performance of the PDE. Present paper introduces the state of the art of PDE subsystem technologies developed in recent years.

• Journal of Pharmacopuncture
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• v.20 no.1
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• pp.45-51
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• 2017

Objectives: This study aimed to evaluate the hypoglycemic effects of an ethanol extract of Cassia abbreviata (ECA) bark and the possible mechanisms of its action in diabetic albino rats. Methods: ECA was prepared by soaking the powdered plant material in 70% ethanol. It was filtered and made solvent-free by evaporation on a rotary evaporator. Type 2 diabetes was induced in albino rats by injecting 35 mg/kg body weight (bw) of streptozotocin after having fed the rats a high-fat diet for 2 weeks. Diabetic rats were divided into ECA-150, ECA-300 and Metformin (MET)-180 groups, where the numbers are the doses in mg.kg.bw administered to the groups. Normal (NC) and diabetic (DC) controls were given distilled water. The animals had their fasting blood glucose levels and body weights determined every 7 days for 21 days. Oral glucose tolerance tests (OGTTs) were carried out in all animals at the beginning and the end of the experiment. Liver and kidney samples were harvested for glucose 6 phosphatase (G6Pase) and hexokinase activity analyses. Small intestines and diaphragms from normal rats were used for ${\alpha}-glucosidase$ and glucose uptake studies against the extract. Results: Two doses, 150 and 300 mg/kg bw, significantly reduced the fasting blood glucose levels in diabetic rats and helped them maintain normal body weights. The glucose level in DC rats significantly increased while their body weights decreased. The 150 mg/kg bw dose significantly increased hexokinase and decreased G6Pase activities in the liver and the kidneys. ECA inhibited ${\alpha}-glucosidase$ activity and promoted glucose uptake in the rats' hemi-diaphragms. Conclusion: This study revealed that ECA normalized blood glucose levels and body weights in type 2 diabetic rats. The normalization of the glucose levels may possibly be due to inhibition of ${\alpha}-glucosidase$, decreased G6Pase activity, increased hexokinase activity and improved glucose uptake by muscle tissues.

• International Journal of Aeronautical and Space Sciences
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• v.13 no.1
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• pp.117-125
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• 2012

This paper presented the design of SRPS, ground function test, and the deployment test on a high speed taxi of KC-100 airplane. KAI has developed a spin recovery system in collaboration with Airborne Systems for KC-100 general aviation airplane. Spin mode analysis, rotary balance and forced oscillation tests were performed to obtain the rotational, dynamic derivatives in the preliminary design phase. Prior to the detailed design process of SRPS, approximations for initial estimation of design parameters- fineness ratio, parachute porosity, parachute canopy filling time, and deployment method- were considered. They were done based on the analytical disciplines such as aerodynamics, structures, and stability & control. SRPS consists of parachute, tractor rocket assembly for deployment, attach release mechanism (ARM) and cockpit control system. Before the installation of SRPS in KC-100 airplane, all the control functions of this system were demonstrated by using SBTB(System Breakout Test Box) in the laboratory. SBTB was used to confirm if it can detect faults, and simulate the firing of pyrotechnic devices that control the deployment and jettison of SRPS. Once confirmed normal operation of SRPS, deployment and jettison of parachute on the high speed taxiing were performed.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.8 no.3
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• pp.229-238
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• 2015

This study was conducted to develop electronic control system of weeding implement that can operated at various areas such as slope land and inclined road side. The weeding implement consists of five main parts; the electronic control system, the hydraulic system, the main frame, the boom and arm mechanism, and the rotary type weeder. And the weeding implement was developed to be attached by three-point hitch of tractor considering the use of electronic control system. As a result, the electronic control test was conducted with the weeding implement attached to tractor in slope land. The results of the electronic control system test showed satisfactory weeding performance.

• Journal of Microbiology and Biotechnology
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• v.29 no.8
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• pp.1288-1298
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• 2019

Bacterial ATP synthases drive ATP synthesis by a rotary mechanism, and play a vital role in physiology and cell metabolism. Corynebacterium glutamicum is well known as an industrial workhorse for amino acid production, and its ATP synthase operon contains eight structural genes and two adjacent genes, cg1360 and cg1361. So far, the physiological functions of Cg1360 (GenBank CAF19908) and Cg1361 (GenBank CAF19909) remain unclear. Here, we showed that Cg1360 was a hydrophobic protein with four transmembrane helices (TMHs), while no TMH was found in Cg1361. Deletion of cg1360, but not cg1361, led to significantly reduced cell growth using glucose and acetic acid as carbon sources, reduced F1 portions in the membrane, reduced ATP-driven proton-pumping activity and ATPase activity, suggesting that Cg1360 plays an important role in ATP synthase function. The intracellular ATP concentration in the ${\Delta}cg1360$ mutant was decreased to 72% of the wild type, while the NADH and NADPH levels in the ${\Delta}cg1360$ mutant were increased by 29% and 26%, respectively. However, the ${\Delta}cg1361$ mutant exhibited comparable intracellular ATP, NADH and NADPH levels with the wild-type strain. Moreover, the effect of cg1360 deletion on L-valine production was examined in the L-valine-producing V-10 strain. The final production of L-valine in the $V-10-{\Delta}cg1360$ mutant reached $9.2{\pm}0.3g/l$ in shake flasks, which was 14% higher than that of the V-10 strain. Thus, Cg1360 can be used as an effective engineering target by altering energy metabolism for the enhancement of amino acid production in C. glutamicum.

• Korean Journal of Veterinary Research
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• v.13 no.2
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• pp.147-160
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• 1973

The present experiments proposed to pass judgement upon the suitability as the second intermediate host of Clonorchis sinensis, of three kinds of fresh-water fish by having them infected with the cercaria of Clonorchis sinensis and then observing the penetrating ability of the cercaria, and maturity, the process of degeneration and extinction, and infectivity of the metacercaria of Clonorchis sinensis. The following is a brief summary of the leading facts gained through the experiments; 1. P. parva was easily subject to invasion of the cercaria, A. signifer was much less subject to the invasion, and finally A. asmussi was hard to invade. And the infectivity of the cercariae was in proportion to their penetrating ability mentioned in the above. 2. The examination of the cercariae after having three kinds of fresh-water fish infected with them disclosed that 24 hours after the infection, all of the cercariae formed their cysts in muscle and the metacercariae kept growing, and that 7 days later the metacercariae were found folding their bodies twice, and that in 15 days the characteristic structure of the metacercariae was complete and they made a vigorous rotary movement intermittently. 3. Then the metacercariae came to a state of maturity and beyond this stage some metacercariae in P. parva started the process of degeneration and extinction in 133 days; some in A. asmussi, in 140 days; and A. signifer, in 70 days. As more days elapsed, their degeneration and extinction increased in number, and in 269 days all of them in A. signifer became dead while those in A. asmussi were all dead in 460 days. However almost all of them in P. parva survived even after 770 days. The results shown above revealed that P. parva was the most suitable as the second intermediate host among three kinds of fresh-water fish and that A. signifer and A. asmussi were not quite recommendable as the second intermediate host. The ability of the cercaria to invade fresh-water fish, and life span of the metacercaria within fresh-water fish vary outstandingly according to species of fresh-water fish. An explanation as to the mechanism must wait as the subject to be further pursued.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.10 no.4
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• pp.659-666
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• 2006

Servo-motors are used as key components of automated system by performing precise motion control as accurate positioning and accurate speed regulation in response to the commands from computers and sensors. Especially, the linear brushless servo-motors have numerous advantages over the rotary servo motors which have connection with the friction induced transfer mechanism such as ball screws, timing belts, rack/pinion. This paper proposes an estimation method of unknown motor system parameters using the informations from the sinusoidal driving type linear brushless DC motor dynamics and outputs. The estimated parameters can be used to tune the controller gain and a disturbance observer. In order to meet this purpose high performance Digital Signal Processor, TMS320F240, designed originally for implementation of a Field Oriented Control(FOC) technology is adopted as a controller of the liner BLDC servo motor. Having A/D converters, PWM generators, rich I/O port internally, this servo motor application specific DSP play an important role in servo motor controller. This linear BLDC servo motor system also contains IPM(Intelligent Power Module) driver and hail sensor type current sensor module, photocoupler module for isolation of gate signals and fault signals.

• Journal of Practical Agriculture & Fisheries Research
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• v.5 no.1
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• pp.64-76
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• 2003

This study was conducted in order to find out the basic data and informations for the accidents for repairing of agricultural machines. This study was summarized as fellows : 1. Among the total repair work, the ratio of repair accident was 68.9% and the accident ratio with power tiller, tractor, and combine was 84.8%. The accident occurred frequently in April to May and September to October because of frequent use of machines during this months. The accidents occurred often of ten to eleven and two to three afternoon in a day. 2. The 36.3% of the accident types was farming machines and the 60.4% of the accident occurred in yard of repair shop. The 34.4% of accident was caused by people, 26.2% by machines, 24.9% by environmental factors, and 14.5% by others. 3. In accident damage, human damage was 98.4% and economic damage was 43.6%. There was only 40.2% in having agricultural machinery insurance and 22.5% of research applicant answered that they would not have insurance in the future. 4. The 58.2% of the injured parts occurred in fingers, hands, wrists, or arms and the 74.4% of the damage types were bruise, prick or abrasion. The damaged parts of machinery were belt of power transmission device, chains, sharpened knives, narrow mechanism or cultivating knives for rotary. 5. The average days of medical treatment for casualty was 15.5 days and the average expense of medical treatment per person was 189,200 won. The days of temporary rest and the economic damage per person due to accident were 12.8 days and 469,300 won.