• International Journal of Oral Biology
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• 제40권2호
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• pp.103-109
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• 2015

Growing evidence suggests that mitochondrial reactive oxygen species (ROS) are involved in various pain states. This study was performed to investigate whether ROS-induced changes in neuronal excitability in trigeminal subnucleus caudalis are related to ROS generation in mitochondria. Confocal scanning laser microscopy was used to measure ROS-induced fluorescence intensity in live rat trigeminal caudalis slices. The ROS level increased during the perfusion of malate, a mitochondrial substrate, after loading of 2',7'-dichlorofluorescin diacetate ($H_2DCF-DA$), an indicator of the intracellular ROS; the ROS level recovered to the control condition after washout. When pre-treated with phenyl N-tert-butylnitrone (PBN) and 4-hydroxy-2,2,6,6-tetramethylpiperidene-1-oxyl (TEMPOL), malate-induced increase of ROS level was suppressed. To identify the direct relation between elevated ROS levels and mitochondria, we applied the malate after double-loading of $H_2DCF-DA$ and chloromethyl-X-rosamine (CMXRos; MitoTracker Red), which is a mitochondria-specific fluorescent probe. As a result, increase of both intracellular ROS and mitochondrial ROS were observed simultaneously. This study demonstrated that elevated ROS in trigeminal subnucleus caudalis neuron can be induced through mitochondrial-ROS pathway, primarily by the leakage of ROS from the mitochondrial electron transport chain.

• 대한생식의학회:학술대회논문집
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• 대한불임학회 2000년도 제39차 춘계 학술대회
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• pp.29-35
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• 2000

The imbalance between reactive oxygen species (ROS) production and total antioxidant capacity (TAC) in seminal fluid indicates oxidative stress and is correlated with male infertility. A composite ROS-TAC score may be more strongly correlated with infertility than ROS or TAC alone. We measured ROS, TAC, and ROS-TAC scores in semen from 127 patients and 24 healthy controls. Of the patients, 56 had varicocele, eight had varicocele with prostatitis, 35 had vasectomy reversals, and 28 had Idiopathic infertility. ROS levels were higher among infertile men, especially those with varicocele with prostatitis (mean ${\pm}$ SE, 3.25 ${\pm}$ 0.89) and vasectomy reversals (2.65 ${\pm}$ 1.01). All infertility groups had significantly lower ROS-TAC scores than control. ROS-TAC score identified 80% of patients and was significantly better than ROS at identifying varicocele and idiopathic infertility. The 13 patients whose partners later achieved pregnancies had a mean ROS-TAC score of 47.7 ${\pm}$ 13.2, similar to controls but significantly higher than the 39 patients who remained infertile (35.8 ${\pm}$ 15.0; P < 0.01). ROS-TAC score is a novel measure of oxidative stress and Is superior to ROS or TAC alone in discriminating between fertile and infertile men. Infertile men with male factor or idiopathic diagnoses had significantly lower ROS-TAC scores than controls, and men with male factor diagnoses that eventually were able to initiate a successful pregnancy had significantly higher ROS-TAC scores than those who failed.

• 정보보호학회논문지
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• 제34권4호
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• pp.681-691
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• 2024

최근 수년 동안 무인지상차량 및 무인항공기와 같은 Robotics 분야에 대한 연구 및 개발이 활발히 진행되고 있다. 이러한 발전에는 서로 다른 애플리케이션 간의 통신 및 데이터 관리를 원활하게 해주는 미들웨어의 사용이 중요한 역할을 하고 있으며, 여러 가지의 산업용 통신 미들웨어 프로토콜들이 출시되고 있다. 그중 ROS (Robot Operating System)가 로봇 시스템 개발을 위한 주요 플랫폼으로 널리 사용되고 있지만, 초기 설계 과정에서 보안 측면을 전혀 고려하지 않았기 때문에 통신을 도청하거나 악의적인 메시지를 주입하는 등의 다양한 공격에 취약한 상태이다. 이에 대응하기 위해, ROS에 대한 보안 솔루션을 제안하는 많은 연구가 진행되고 있으며, 특히 침입 탐지 시스템을 위한 ROS 데이터셋을 제안하는 연구도 진행되었지만, 이와 같은 연구는 매우 부족한 상황이다. 본 논문에서는 ROS 침입 탐지 시스템의 성능 발전에 기여할 수 있도록 ROS 환경에서 발생 가능한 새로운 유형의 공격 시나리오를 제안하고, 실제 로봇 시스템으로부터 수집한 ROS 공격 데이터셋을 구축하며 오픈 데이터셋으로 제공한다.

• 제어로봇시스템학회논문지
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• 제22권2호
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• pp.153-161
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• 2016

This paper proposes a bridge model for data exchange and service invocation between OPRoS and ROS platforms, shows the validity of the proposed model via applications, and compares the proposed model with the OPRoS platform and the ROS platform using performance measures such as data exchange time and service response time. The proposed model operates independently of OPRoS and ROS Platforms using its configuration file with mapping information among the OPRoS data/service port and the ROS topic/service. The configuration file makes easy connections between OPRoS data/service and ROS topic/service without changing the source code of the platform and components.

• Environmental Analysis Health and Toxicology
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• 제23권1호
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• pp.33-39
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• 2008

The genotoxicity of mercury compounds have been investigated with a variety of genetic endpoints in prokaryotic and eukaryotic cells. The mercury ions are positively charged and easily form complexes with DNA by binding with negatively charged centers to cause mutagenesis. Further, the mercury ions can react with sulfhydryl (-SH) groups of proteins associated with DNA replication and alter genetic information. Another mechanism by which mercury damages DNA molecule is via its probable involvement of reactive oxygen species (ROS) and induces DNA strand breaks. In order to investigate whether the ROS production was induced by mercury, we performed ROS assay. As the result, the ROS production was significantly increased when it grows dose-dependently and time-dependently. We compared mercury alone-treated group and mercury co-treated with Vitamin C or glutathione group. As the result, the ROS production induced by mercury was decreased by Vitamin C and glutathione. Co-treated with Vitamin C and glutathione group was the most effective to lowering ROS production induced by mercury.

• 정보보호학회논문지
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• 제32권6호
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• pp.1091-1102
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• 2022

로봇 시스템은 지난 10년 간 매우 빠른 속도로 발전했다. Robot Operating System은 로봇 시스템 및 애플리케이션의 효율적인 개발을 위한 오픈소스 기반의 소프트웨어 프레임워크이며, 다양한 연구 및 산업 현장에서 널리 사용되고 있다. ROS 애플리케이션은 다양한 취약점을 내재하고 있을 수 있다. 이러한 ROS 애플리케이션의 실행을 런타임 모니터링 하기 위해 다양한 연구가 진행되어 왔다. 본 연구에서는 ROS 2에서의 이벤트 기반 런타임 모니터링을 활용한 실시간 공격 탐지 시스템을 제안한다. 우리의 공격 탐지 시스템은 ros2_tracing의 tracetools를 확장하여 ROS 2 미들웨어 계층의 주요 라이브러리에 이벤트 계측을 삽입하고 런타임 중에 이벤트를 모니터링함으로써API의 비순차적 실행을 통한 애플리케이션 계층에서의 공격을 탐지한다.

• Molecules and Cells
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• 제46권6호
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• pp.329-336
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• 2023

Reactive oxygen species (ROS) serve as secondary messengers that regulate various developmental and signal transduction processes, with ROS primarily generated by NADPH OXIDASEs (referred to as RESPIRATORY BURST OXIDASE HOMOLOGs [RBOHs] in plants). However, the types and locations of ROS produced by RBOHs are different from those expected to mediate intracellular signaling. RBOHs produce O₂^•- rather than H₂O₂ which is relatively long-lived and able to diffuse through membranes, and this production occurs outside the cell instead of in the cytoplasm, where signaling cascades occur. A widely accepted model explaining this discrepancy proposes that RBOH-produced extracellular O₂^•- is converted to H₂O₂ by superoxide dismutase and then imported by aquaporins to reach its cytoplasmic targets. However, this model does not explain how the specificity of ROS targeting is ensured while minimizing unnecessary damage during the bulk translocation of extracellular ROS (eROS). An increasing number of studies have provided clues about eROS action mechanisms, revealing various mechanisms for eROS perception in the apoplast, crosstalk between eROS and reactive nitrogen species, and the contribution of intracellular organelles to cytoplasmic ROS bursts. In this review, we summarize these recent advances, highlight the mechanisms underlying eROS action, and provide an overview of the routes by which eROS-induced changes reach the intracellular space.

• 한국식품영양과학회지
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• 제43권6호
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• pp.822-828
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• 2014

본 연구에서는 3T3-L1 지방전구세포를 이용하여 Allium 속 식물의 하나인 참산부추(ASM) 메탄올 추출물의 ROS 생성 저해 및 지질 축적 억제 효과를 확인하고자 하였다. 먼저 ASM 메탄올 추출물 $100{\sim}2,000{\mu}g/mL$의 모든 농도에서 유의적인 DPPH 라디칼 소거능을 나타내었으며, 지방전구세포에 ASM 메탄올 추출물 $10{\sim}100{\mu}g/mL$를 처리하였을 때 세포 독성을 나타내지 않았다. 지방세포 내 ROS 관련 효소와 분화 관련 전사인자의 조절로 인한 중성지방 축적 저해 활성을 확인하기 위하여 지방전구세포를 분화 유도하면서 추출물을 농도별(10, 50, $100{\mu}g/mL$)로 처리하였다. 그 결과 ASM 메탄올 추출물은 대조군에 비해 ROS 생성량과 ROS 관련 효소인 G6PDH mRNA 발현을 농도 의존적으로 감소시켰다. 또한 ASM 메탄올 추출물에 의하여 지방세포 내 중성지방 축적량이 유의적으로 감소하였으며, 지방세포 분화에 관련된 전사인자인 SREBP1c, $PPAR{\gamma}$ 및 $C/EBP{\alpha}$ mRNA 발현도 유의적으로 감소하였다. 이상의 결과들로 볼 때 ASM 메탄올 추출물로 인한 ROS 생성 저해와 지질 축적 억제는 ROS 생성 및 ROS 관련 유전자의 발현감소로 인한 지방 생성 주요 전사인자의 유전자 발현 억제로 인한 것으로 보이며, ASM이 항비만 효과가 있는 천연물 소재로 개발 가능성이 있을 것으로 사료된다.

• Molecular Medicine Reports
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• 제20권3호
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• pp.2339-2346
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• 2019

Reactive oxygen species (ROS) are important cellular second messengers involved in various aspects of cell signaling. ROS are elevated in multiple types of cancer cells, and this elevation is known to be involved in pathological processes of cancer. Although high levels of ROS exert cytotoxic effects on cancer cells, low levels of ROS stimulate cell proliferation and survival by inducing several pro-survival signaling pathways. In addition, ROS have been shown to induce epithelial-mesenchymal transition (EMT), which is essential for the initiation of metastasis. However, the precise mechanism of ROS-induced EMT remains to be elucidated. In the present study, it was indicated that ROS induce EMT by activating Snail expression, which then represses E- cadherin expression in MCF-7 cells. It was further indicated that distal-less homeobox-2 (Dlx-2), one of the human Dlx gene family proteins involved in embryonic development, acts as an upstream regulator of ROS-induced Snail expression. It was also revealed that ROS treatment induces the glycolytic switch, a phenomenon whereby cancer cells primarily rely on glycolysis instead of mitochondrial oxidative phosphorylation for ATP production, even in the presence of oxygen. In addition, ROS inhibited oxidative phosphorylation and caused cytochrome c oxidase inhibition via the Dlx-2/Snail cascade. These results suggest that ROS induce EMT, the glycolytic switch and mitochondrial repression by activating the Dlx-2/Snail axis, thereby playing crucial roles in MCF-7 cancer cell progression.

• Biomolecules & Therapeutics
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• 제28권1호
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• pp.104-109
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• 2020

Probenecid is a representative drug used in the treatment of gout. A recent study showed that probenecid effectively inhibits oxidative stress in neural cells. In the present study, we investigated whether probenecid can affect osteoclast formation through the inhibition of reactive oxygen species (ROS) formation in RAW264.7 cells. Lipopolysaccharide (LPS)-induced ROS levels were dose-dependently reduced by probenecid. Fluorescence microscopy analysis clearly showed that probenecid inhibits the generation of ROS. Western blot analysis indicated that probenecid affects two downstream signaling molecules of ROS, cyclooxygenase 2 (COX-2) and c-Jun N-terminal kinase (JNK). These results indicate that probenecid inhibits ROS generation and exerts antiosteoclastogenic activity by inhibiting the COX-2 and JNK pathways. These results suggest that probenecid could potentially be used as a therapeutic agent to prevent bone resorption.