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Effect of Vitamin C and GSH on the Hg Induced ROS  

Kwon, Kyoung-Jin (College of Pharmacy, Ewha Womans University)
Sheen, Yhun-Yhong (College of Pharmacy, Ewha Womans University)
Publication Information
Environmental Analysis Health and Toxicology / v.23, no.1, 2008 , pp. 33-39 More about this Journal
Abstract
The genotoxicity of mercury compounds have been investigated with a variety of genetic endpoints in prokaryotic and eukaryotic cells. The mercury ions are positively charged and easily form complexes with DNA by binding with negatively charged centers to cause mutagenesis. Further, the mercury ions can react with sulfhydryl (-SH) groups of proteins associated with DNA replication and alter genetic information. Another mechanism by which mercury damages DNA molecule is via its probable involvement of reactive oxygen species (ROS) and induces DNA strand breaks. In order to investigate whether the ROS production was induced by mercury, we performed ROS assay. As the result, the ROS production was significantly increased when it grows dose-dependently and time-dependently. We compared mercury alone-treated group and mercury co-treated with Vitamin C or glutathione group. As the result, the ROS production induced by mercury was decreased by Vitamin C and glutathione. Co-treated with Vitamin C and glutathione group was the most effective to lowering ROS production induced by mercury.
Keywords
mercury; Vitamin C; GSH; reactive oxygen species;
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1 Chavez E and Holguin JA. Mitochondrial calcium release as induced by $Hg^{2+}$, J Biol Chem 1988; 263(8): 3582-3587
2 Lee YW, Ha MS and Kim YK. Role of reactive oxygen species and glutathione in inorganic mercury-induced injury in human glioma cells, Neurochem Res 2001; 26(11):1187-1193   DOI   ScienceOn
3 Shenker BJ, Mayro JS, Rooney C, Vitale L and Shapiro IM. Immunotoxic effects of mercuric compounds on human lymphocytes and monocytes. IV. Alterations in cellular glutathione content, Immunopharmacol Immunotoxicol 1993; 15(2-3): 273-290   DOI
4 Castillo J, Benavente-Garcia O, Lorente J, Alcaraz M, Redondo A, Ortuno A and Del Rio JA. Antioxidant activity and radioprotective effects against chromosomal damage induced in vivo by X-rays of flavan-3-ols (Procyanidins) from grape seeds (Vitis vinifera): comparative study versus other phenolic and organic compounds, J Agric Food Chem 2000; 48(5): 1738-1745   DOI   ScienceOn
5 Nath KA, Croatt AJ, Likely S, Behrens TW and Warden D. Renal oxidant injury and oxidant response induced by mercury, Kidney Int 1996; 50(3): 1032-1043   DOI   ScienceOn
6 Antunes LM and Takahashi CS. Protection and induction of chromosomal damage by vitamin C in human lympho-cyte cultures, Teratog Carcinog Mutagen 1999; 19(1):53-59   DOI   ScienceOn
7 Clarkson TW. The three modern faces of mercury, Environ Health Perspect 110 Suppl 2002; 1: 11-23
8 Gebhart E and Rossman TG. Mutagenicity, carcinogenicity and teratogenicity. in: E. Merian (Ed.), Metals and their Compounds in the Environment. VCH, New York 1991
9 Guille E, Grisvard J and Sissoeff I. Structure and function of metallo-DNA in the living cells. Systemic aspects of Biocompatibility. in: D. F. Williams (Ed.), 1. CRC press, FL. 1981
10 Kojima S, Shimada H and Kiyozumi M. Comparative effects of chelating agents on distribution, excretion, and renal toxicity of inorganic mercury in rats, Res Commun Chem Pathol Pharmacol 1989; 64(3): 471-484
11 Zalups RK. Molecular interactions with mercury in the kidney, Pharmacol 2000; Rev 52(1): 113-143   DOI
12 WHO. Environmental Health Criteria, 118: Inorganic Mercury Environmental Aspects. 1991
13 Sweetman SF, Strain JJ and McKelvey-Martin VJ. Effect of antioxidant vitamin supplementation on DNA damage and repair in human lymphoblastoid cells, Nutr Cancer 1997; 27(2): 122-130
14 Konopacka M, Widel M and Rzeszowska-Wolny J. Modifying effect of vitamins C, E and beta-carotene against gamma-ray-induced DNA damage in mouse cells, Mutat Res 1998; 417(2-3): 85-94   DOI
15 Patrick L. Mercury toxicity and antioxidants: Part 1: role of glutathione and alpha-lipoic acid in the treatment of mercury toxicity, Altern Med Rev 2002; 7(6): 456-471
16 Ermak G and Davies KJ. Calcium and oxidative stress: from cell signaling to cell death, Mol Immunol 2002; 38(10):713-721   DOI   ScienceOn
17 Queiroz ML, Pena SC, Salles TS, de Capitani EM and Saad ST. Abnormal antioxidant system in erythrocytes of mercury-exposed workers, Hum Exp Toxicol 1998; 17(4): 225-230   DOI   ScienceOn
18 National Research Council. Toxicological effects of Methylmercury. National Academy Press, Washington, DC. 2000
19 Green MH, Lowe JE, Waugh AP, Aldridge KE, Cole J and Arlett CF. Effect of diet and vitamin C on DNA strand breakage in freshly-isolated human white blood cells, Mutat Res 1994; 316(2): 91-102   DOI   ScienceOn
20 Eylar E, Baez I, Navas J and Mercado C. Sustained levels of ascorbic acid are toxic and immunosuppressive for human T cells, P R Health Sci J 1996; 15(1): 21-26
21 Yee S and Choi BH. Oxidative stress in neurotoxic effects of methylmercury poisoning, Neurotoxicology 1996; 17(1): 17-26
22 Herbaczynska-Cedro K, BK o-W, Cedro K, Wasek W, Panczenko-Kresowska B and Wartanowicz M. Supplementation with vitamins C and E suppresses leukocyte oxygen free radical production in patients with myocardial infarction, Eur Heart J 1995; 16(8): 1044-9   DOI
23 Hoda Q and Sinha SP. Vitamin C-mediated minimisation of Rogor-induced genotoxicity, Mutat Res 1993; 299(1):29-36   DOI   ScienceOn
24 Ozuah PO. Mercury poisoning, Curr Probl Pediatr 2000; 30(3): 91-99   DOI   ScienceOn
25 Chang WK, Yang KD and Shaio MF. Lymphocyte proliferation modulated by glutamine: involved in the endogenous redox reaction, Clin Exp Immunol 1999; 117(3):482-488   DOI
26 Kromidas L, Trombetta LD and Jamall IS. The protective effects of glutathione against methylmercury cytotoxicity, Toxicol Lett 1990; 51(1): 67-80   DOI   ScienceOn
27 Chorvatovicova D, Ginter E, Kosinova A and Zloch Z. Effect of vitamins C and E on toxicity and mutagenicity of hexavalent chromium in rat and guinea pig, Mutat Res 1991; 262(1): 41-46   DOI   ScienceOn
28 Lund BO, Miller DM and Woods JS. Mercury-induced $H_2O_2$ production and lipid peroxidation in vitro in rat kidney mitochondria, Biochem Pharmacol 1991; 42 Suppl:S181-187