• Proceedings of the Korean Institute of Building Construction Conference
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• 2009.05c
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• pp.89-97
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• 2009

As landscaping building roofs and concrete structures increase gradually for low carbon green growth policy of government, But waterproofing membranes of those structures are effecting by root penetration of landscape plant. 80, we progressed study about test method for fast evaluating root resistance of waterproofing materials jointly with Tokyo Institute of Technology. The result of the study is as follows: (1) The penetrating load of the needle at the displacement speed of 1mm/min was measured for various membrane to basis and lap joints of membrane, the load force was $3{\sim}50$ N by material variously. (2) According to the test method of deriving rhizomes of bamboo grass to basis and lap joints of membrane, there were no penetrated membrane until present, but need persistent observation. (3) Test method of deriving rhizomes of bamboo grass to basis and lap joints of membrane can shorten from period of 2 years to 1 year for testing. Because rhizomes of bamboo grass can grow from May to September, test is possible in same period.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2009.11a
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• pp.141-148
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• 2009

The purpose of this paper is to propose a standard test methods of resistance to root penetration for waterproofing and rootproofing membrane using green roof system. Green roof system is considered to be an important subject in construction industry for green growth project. At the same time, we have to consider the counterplan for protection the damage of waterproofing layer and concrete substrate from the penetration of plant root. But many kinds of materials for protection from root penetration are using in construction field. But the performance of those materials is not clear, and there is not test methods for the evaluation of performance. So in this paper, based on the research results of 4 institutes during four years and foreign cases, we made a standard test methods of resistance to root penetration for waterproofing and rootproofing membrane using green roof system. This test method deals with about environmental condition of laboratory, experimental facilities, kinds of plant, specimen of test, management methods, evaluation duration and documents, etc.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2008.11a
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• pp.173-178
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• 2008

This is green roof bottom system which composed by aluminum valve and glass fiber together as major reinforcement, so the cooper sheet can have root proof, and using recycled tire gel-type membrane waterproofing system which dost not contains VOCs. The copper sheet reduce the plants' root growing, so it helpes to maintain the waterproofing layer and stability of root proofing. Gel type membrane waterproofing system can do waterproofing, stress dispersion, and reducing leakage expansion. So those two materials can help each other to make green roof bottom layer would have the stability and durability.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2018.05a
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• pp.253-254
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• 2018

In the construction industry, a variety of Root-Resistance Waterproofing Sheets ar being developed and studied. However, defects still occur in the sheet. In this study, the performance of the navigation is studied by Root-Resistance. As a result of the test, no piercing or penetration marks were found by root in all the specimens.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.13 no.4
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• pp.42-51
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• 2010

The purpose of this study is to analyze the characteristics of environment-friendliness of Root Barrier materials based on comprehensive experiments on harmfulness of Root Barrier materials and fish toxicity of Oryzias latipes mortality to verify eco-toxicity of each method of construction and Root Barrier material, which are to be applied by taking eco-toxicity into account when building ecological flows in upper areas on natural and artificial grounds. As a result, the following conclusions have been reached in this study: 1. In regard of the harmfulness analyzed, each material showed a different result of analytical value in each lab tank. Compared to lab tank, pH (la), DO (lb), T-N (VI) and T-coli (la) were in the same grade, but COD, SS, T-P and F-coli were less than that of control, respectively increased or decreased by material were analyzed. 2. In the experiment of fish toxicity, Barrier sheet was found to have 66.7% of fish mortality, indicating strong fish toxicity. Synthetic rubber system root barrier sheet (20.0%) was analyzed to have medium fish toxicity, while Synthetic resin system root barrier-waterproof sheet (3.3%), Synthetic rubber system membrane root barrier sheet (3.3%) and Synthetic resin system root barrier sheet (0.0%) showed relatively lower mortality and fish toxicity. To sum up such results as found in the experiment mentioned so far, the values of harmfulness and root penetration resistance analyzed were different in each lab tank, but there was absolutely little correlation with the mortality gained from the experiment on fish toxicity. In the experiment of fish toxicity, environment-friendly root barrier materials were analyzed, and it was found that Synthetic resin system root barrier sheet, Synthetic resin system root barrier waterproof sheet and Synthetic rubber system membrane root barrier sheet are highly environment-friendly. In contrast, Synthetic rubber system root barrier sheet was found to have medium-level environment-friendliness. Also, Barrier sheet was analyzed to have low environment-friendliness.

• The Plant Pathology Journal
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• v.35 no.6
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• pp.692-697
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• 2019

Agrobacterium rhizogenes-mediated transformation of sugar beet hairy roots expressing single-chain variable fragment (scFv) was exploited to evaluate the efficacy of four antibody-based constructs for interfering with the Beet necrotic yellow vein virus infection. The scFv specific to a major coat protein of virus, p21, was targeted to various cellular compartments including the cytosol (pIC and pICC constructs), apoplast (pIA), and mitochondrion (pIM). After mechanical virus inoculation, most of the hairy root clones expressing scFv in the cytosol displayed low virus titers while the majority of transgenic hairy root clones accumulated antibody in outer membrane of mitochondria or apoplast were infected. This hairy root system provided an efficient and rapid approach to initially investigating root disease resistance like rhizomania prior to transform whole recalcitrant plants such as sugar beet.

• Journal of the Korea Institute of Building Construction
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• v.11 no.2
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• pp.189-199
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• 2011

Installation of a proper root barrier in a green roof system is very important in order to protect the concrete slab of roof and the root penetration in the waterproofing layer. To select the proper root barrier materials and methods, it is necessary to understand the environmental conditions affecting the waterproofing-root barrier system in green roof construction site. Therefore, we suggested as the environmental performance indexes four kinds of performance requirements; root penetration, chemical attack by chemical agent or fertilizer, load impact by soil depth and size of plant, and water pressure. The related four test methods were suggested for the inspection of these performance indexes. In this research, we could suggest for kinds of test methods as standard test methods to evaluate the environmental performance of waterproofing-root barrier for greening roof system.

• Biomolecules & Therapeutics
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• v.32 no.1
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• pp.104-114
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• 2024

Licochalcone C (LCC; PubChem CID:9840805), a chalcone compound originating from the root of Glycyrrhiza inflata, has shown anticancer activity against skin cancer, esophageal squamous cell carcinoma, and oral squamous cell carcinoma. However, the therapeutic potential of LCC in treating colorectal cancer (CRC) and its underlying molecular mechanisms remain unclear. Chemotherapy for CRC is challenging because of the development of drug resistance. In this study, we examined the antiproliferative activity of LCC in human colorectal carcinoma HCT116 cells, oxaliplatin (Ox) sensitive and Ox-resistant HCT116 cells (HCT116-OxR). LCC significantly and selectively inhibited the growth of HCT116 and HCT116-OxR cells. An in vitro kinase assay showed that LCC inhibited the kinase activities of EGFR and AKT. Molecular docking simulations using AutoDock Vina indicated that LCC could be in ATP-binding pockets. Decreased phosphorylation of EGFR and AKT was observed in the LCC-treated cells. In addition, LCC induced cell cycle arrest by modulating the expression of cell cycle regulators p21, p27, cyclin B1, and cdc2. LCC treatment induced ROS generation in CRC cells, and the ROS induction was accompanied by the phosphorylation of JNK and p38 kinases. Moreover, LCC dysregulated mitochondrial membrane potential (MMP), and the disruption of MMP resulted in the release of cytochrome c into the cytoplasm and activation of caspases to execute apoptosis. Overall, LCC showed anticancer activity against both Ox-sensitive and Ox-resistant CRC cells by targeting EGFR and AKT, inducing ROS generation and disrupting MMP. Thus, LCC may be potential therapeutic agents for the treatment of Ox-resistant CRC cells.