The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Antibody-Mediated Resistance to Rhizomania Disease in Sugar Beet Hairy Roots

  • Jafarzade, M. (Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology) ;
  • Ramezani, M. (Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology) ;
  • Hedayati, F. (Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology) ;
  • Mokhtarzade, Z. (Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology) ;
  • Zare, B. (Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology) ;
  • Sabet, M.S. (Department of Agriculture, Tarbiat Modares University) ;
  • Norouzi, P. (Sugar Beet Seed Institute, Agricultural Research, Education and Extension Organization (AREEO)) ;
  • Malboobi, M.A. (Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology)
  • Received : 2018.06.17
  • Accepted : 2018.10.01
  • Published : 2019.12.01
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Abstract

Agrobacterium rhizogenes-mediated transformation of sugar beet hairy roots expressing single-chain variable fragment (scFv) was exploited to evaluate the efficacy of four antibody-based constructs for interfering with the Beet necrotic yellow vein virus infection. The scFv specific to a major coat protein of virus, p21, was targeted to various cellular compartments including the cytosol (pIC and pICC constructs), apoplast (pIA), and mitochondrion (pIM). After mechanical virus inoculation, most of the hairy root clones expressing scFv in the cytosol displayed low virus titers while the majority of transgenic hairy root clones accumulated antibody in outer membrane of mitochondria or apoplast were infected. This hairy root system provided an efficient and rapid approach to initially investigating root disease resistance like rhizomania prior to transform whole recalcitrant plants such as sugar beet.

Keywords

References

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