• Molecules and Cells
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• 제46권12호
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• pp.764-777
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• 2023

Recombinant immunotoxins (RITs) are fusion proteins consisting of a targeting domain linked to a toxin, offering a highly specific therapeutic strategy for cancer treatment. In this study, we engineered and characterized RITs aimed at mesothelin, a cell surface glycoprotein overexpressed in various malignancies. Through an extensive screening of a large nanobody library, four mesothelin-specific nanobodies were selected and genetically fused to a truncated Pseudomonas exotoxin (PE24B). Various optimizations, including the incorporation of furin cleavage sites, maltose-binding protein tags, and tobacco etch virus protease cleavage sites, were implemented to improve protein expression, solubility, and purification. The RITs were successfully overexpressed in Escherichia coli, achieving high solubility and purity post-purification. In vitro cytotoxicity assays on gastric carcinoma cell lines NCI-N87 and AGS revealed that Meso(Nb2)-PE24B demonstrated the highest cytotoxic efficacy, warranting further characterization. This RIT also displayed selective binding to human and monkey mesothelins but not to mouse mesothelin. The competitive binding assays between different RIT constructs revealed significant alterations in IC₅₀ values, emphasizing the importance of nanobody specificity. Finally, a modification in the endoplasmic reticulum retention signal at the C-terminus further augmented its cytotoxic activity. Our findings offer valuable insights into the design and optimization of RITs, showcasing the potential of Meso(Nb2)-PE24B as a promising therapeutic candidate for targeted cancer treatment.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2004년도 ICCAS
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• pp.211-214
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• 2004

Microstrip slot array antenna fed by microstrip line is introduced. Slot antenna is designed to operate at 10 GHz for using in radar systems. Antenna have dielectric constant of the substrate is 2.17 (PTFE). In fact, it is study to analyze slot array antenna including feeding line with wide bandwidth. The characteristics of antenna is proposed and analyzed for instance input impedance, $S_{11}$ parameter and far field radiation patterns which these characteristics can also be calculated efficiently and accurately by using FDTD Method.

• Journal of Microbiology and Biotechnology
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• 제28권2호
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• pp.347-347
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• 2018

• 복식
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• 제25권
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• pp.269-284
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• 1995

This study investgates the significance of Chosun burial clothing in 15th to 17th Centuries by examing the costumes recorded in the ryesu (ritual books). Referring to description of the mouring-rit-ual in the the Kujoohryei(국조오례의), the sangryebiyo(상례비요), they exhibit little differ-ence in the ceremonial procedure, this implies that the the Jujagarye, the oldiest Chinese Ryesu, had influence on the manner of Chosun. The Kujoohryei(국조오례의) written-in 15th Century played a paramount role in domesti-cation of burial costume(염습의) which had been performed by the Jujagarye(주자가례), and the sangryebiyo(상례비요) etc written between 16th and 17th Centries promoted to genealize the mouring-ritual to the ordinery people. In the period, ryemsupui(염습의, clothing for the dead) is costumes for 'sup(습)', sofyum(소렴), and 'daeryum(대렴)' in the mourning ceremorial procedure, and for postliminal rites in the rites of passage. The reason of using clothing which they put on the dead's own daily clothing stands for eagerness for lasting life not only in this world but also in the other world.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2004년도 ICCAS
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• pp.321-325
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• 2004

Retinopathy of Prematurity (ROP) is a common retinal neovascular disorder of premature infants. It can be characterized by inappropriate and disorganized vessel. This paper present a method for blood vessel detection on infant retinal images. The algorithm is designed to detect the retinal vessels. The proposed method applies a Lapalacian of Gaussian as a step-edge detector based on the second-order directional derivative to identify locations of the edge of vessels with zero crossings. The procedure allows parameters computation in a fixed number of operations independent of kernel size. This method is composed of four steps : grayscale conversion, edge detection based on LOG, noise removal by adaptive Wiener filter & median filter, and Otsu's global thresholding. The algorithm has been tested on twenty infant retinal images. In cooperation with the Digital Imaging Research Centre, Kingston University, London and Department of Opthalmology, Imperial College London who supplied all the images used in this project. The algorithm has done well to detect small thin vessels, which are of interest in clinical practice.

• 대한미생물학회지
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• 제21권2호
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• pp.243-249
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• 1986

A study of the immunogenicity and reactogenicity of two doses of lot H(10, 20 mcg), two doses of lot L (20, 40 mcg) of the Smith Kline-RIT recombinant DNA yeast-derived hepatitis B vaccine and a 20-mcg dose of the Merck Sharp and Dohme plasma-derived hepatitis B vaccine was conducted in young adults under randomized, double-blind conditions. Immunization was carried out according to a 0-, 1-, and 6-month vaccination schedule. Results indicated that the yeast-derived hepatitis B vaccine was well tolerated and immunogenic. Reactogenicity to both yeast- and plasma-derived vaccines was mild in severity and low in incidence with no significant differences appearing between the study groups. One month after the third dose, the yeast-derived vaccines induced a high degree of soroconversion ranging between 95.0% and 100%. The response was not lot or dose-dependent. The administration of the plasma-derived vaccine resulted in anti-HBs geometric mean titres statistically signifirantly higher than those elicited by the different yeast-derived hepatitis B vaccines one month after the third dose of vaccine but the difference was not large enough to be of great clinical significance.

• 전자공학회논문지SC
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• 제48권5호
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• pp.93-98
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• 2011

본 논문에서는 스마트폰을 이용하여 원격감시 및 로봇제어를 위하여 로봇기술(RT, Robot Technology)과 정보기술(IT, Information Technology)의 융합기술인 RITS을 활용하여 구현된 정찰 및 전투 로봇의 프레임워크를 제안한다. 구현된 시스템에서 로봇에 장착된 카메라폰은 로봇을 제어하는데 활용할 뿐만 아니라 원격지에 있는 운용자의 스마트폰으로 영상정보를 전송한다. 따라서 운용자는 스마트폰의 주변지역을 정찰할 수 있다. 또한 운용자는 스마트폰을 이용하여 로봇의 움직임을 제어할 뿐만 아니라, 스마트폰으로 명령을 내려 로봇에 장착한 무기를 발사할 수 있다. 실험결과, 3세대 이동통신을 이용하여 원격지에서 실시간으로 로봇의 주변 환경을 정찰하고 로봇을 제어 할 수 있을 뿐 아니라, 시야에서 관찰되는 영역에 로봇 있을 경우 2세대 이동통신이나 유선 전화기를 이용하여 실시간으로 로봇을 제어할 수 있다.

• 대한의생명과학회지
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• 제21권1호
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• pp.23-31
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• 2015

Recently, specific antibodies have been used extensively to diagnose and treat various diseases. It is essential to assess the efficacy and specificity of antibodies, especially the in vivo environment. Anti-HER-2/neu mAb was evaluated as a possible transporting agent for radioimmunotherapy. The monoclonal antibody was successfully radio-labeled with $^{131}I$. In vitro binding assays were performed to confirm its targeting ability using another radio-iodine, $^{125}I$. Binding percentage of $^{125}I$ labeled anti-HER-2/neu mAb in HER-2/neu expressing CT-26 cells was found to be 4.5%, whereas the binding percentage of $^{125}I$ labeled anti-HER-2/neu mAb in wild-type CT-26 was only 0.45%. In vivo images were obtained and analyzed through $\gamma$-camera and an optical fluorescent modality, IVIS-200. $\gamma$-camera images showed that $^{131}I$ labeled anti-HER-2/neu mAb accumulated in HER-2/neu CT-26 tumors. Optical imaging based on near infrared fluorescence labeled anti-HER-2/neu mAb showed higher fluorescence intensities in HER-2/neu CT-26 tumors than in wild-type CT-26 tumors. Anti-HER-2/neu mAb was found to specifically bind to its receptor expressing tumor. Our study demonstrates that in vivo imaging technique is a useful method for the evaluation of an antibody's therapeutic and diagnostic potentials.

• 한국의학물리학회지:의학물리
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• 제29권2호
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• pp.53-58
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• 2018

This paper evaluates patient-specific quality assurance (PSQA) in the treatment of small and multiple tumors by the CyberKnife system with fixed collimators, using an ion chamber and EBT3 films. We selected 49 patients with single or multiple brain tumors, and the treatment plans include one to four targets with total volumes ranging from 0.12 cc to 3.74 cc. All PSQA deliveries were performed with a stereotactic dose verification phantom. The A16 microchamber (Standard Imaging, WI, USA) and Gafchromic EBT3 film (Ashland ISP Advanced Materials, NJ, USA) were inserted into the phantom to measure the point dose of the target and the dose distribution, respectively. The film was scanned 1 hr after irradiation by a film digitizer scanner and analyzed using RIT software (Radiological Imaging Technology, CO, USA). The acceptance criteria was <5% for the point dose measurement and >90% gamma passing rate using 3%/3 mm and relative dose difference, respectively. The point dose errors between the calculated and measured dose by the ion chamber were in the range of -17.5% to 8.03%. The mean point dose differences for 5 mm, 7.5 mm, and 10 mm fixed cone size was -11.1%, -4.1%, and -1.5%, respectively. The mean gamma passing rates for all cases was 96.1%. Although the maximum dose distribution of multiple targets was not shown in the film, gamma distribution showed that dose verification for multiple tumors can be performed. The use of the microchamber and EBT3 film made it possible to verify the dosimetric and mechanical accuracy of small and multiple targets. In particular, the correction factors should be applied to small fixed collimators less than 10 mm.

• Journal of Power Electronics
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• 제13권1호
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• pp.139-160
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• 2013

In this paper, an intelligent robust control system (IRCS) for precision tracking control of permanent-magnet synchronous motor (PMSM) servo drives is proposed. The IRCS comprises a recurrent wavelet-based interval type-2 fuzzy-neural-network controller (RWIT2FNNC), an RWIT2FNN estimator (RWIT2FNNE) and a compensated controller. The RWIT2FNNC combines the merits of a self-constructing interval type-2 fuzzy logic system, a recurrent neural network and a wavelet neural network. Moreover, it performs the structure and parameter-learning concurrently. The RWIT2FNNC is used as the main tracking controller to mimic the ideal control law (ICL) while the RWIT2FNNE is developed to approximate an unknown dynamic function including the lumped parameter uncertainty. Furthermore, the compensated controller is designed to achieve $L_2$ tracking performance with a desired attenuation level and to deal with uncertainties including approximation errors, optimal parameter vectors and higher order terms in the Taylor series. Moreover, the adaptive learning algorithms for the compensated controller and the RWIT2FNNE are derived by using the Lyapunov stability theorem to train the parameters of the RWIT2FNNE online. A computer simulation and an experimental system are developed to validate the effectiveness of the proposed IRCS. All of the control algorithms are implemented on a TMS320C31 DSP-based control computer. The simulation and experimental results confirm that the IRCS grants robust performance and precise response regardless of load disturbances and PMSM parameters uncertainties.