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http://dx.doi.org/10.15616/BSL.2015.21.1.23

The Characterization of Anti-HER-2/neu Monoclonal Antibody using Different in vivo Imaging Techniques  

Moon, Cheol (Department of Clinical Laboratory Science, Semyung University)
Kim, Eun Jung (Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS))
Choi, Dan Bee (Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS))
Kim, Byoung Soo (Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS))
Kim, Sa Hyun (Department of Clinical Laboratory Science, Semyung University)
Choi, Tae Hyun (Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS))
Publication Information
Biomedical Science Letters / v.21, no.1, 2015 , pp. 23-31 More about this Journal
Abstract
Recently, specific antibodies have been used extensively to diagnose and treat various diseases. It is essential to assess the efficacy and specificity of antibodies, especially the in vivo environment. Anti-HER-2/neu mAb was evaluated as a possible transporting agent for radioimmunotherapy. The monoclonal antibody was successfully radio-labeled with $^{131}I$. In vitro binding assays were performed to confirm its targeting ability using another radio-iodine, $^{125}I$. Binding percentage of $^{125}I$ labeled anti-HER-2/neu mAb in HER-2/neu expressing CT-26 cells was found to be 4.5%, whereas the binding percentage of $^{125}I$ labeled anti-HER-2/neu mAb in wild-type CT-26 was only 0.45%. In vivo images were obtained and analyzed through $\gamma$-camera and an optical fluorescent modality, IVIS-200. $\gamma$-camera images showed that $^{131}I$ labeled anti-HER-2/neu mAb accumulated in HER-2/neu CT-26 tumors. Optical imaging based on near infrared fluorescence labeled anti-HER-2/neu mAb showed higher fluorescence intensities in HER-2/neu CT-26 tumors than in wild-type CT-26 tumors. Anti-HER-2/neu mAb was found to specifically bind to its receptor expressing tumor. Our study demonstrates that in vivo imaging technique is a useful method for the evaluation of an antibody's therapeutic and diagnostic potentials.
Keywords
Radioimmunotherapy (RIT); In vivo imaging; Monoclonal antibody; HER-2/neu oncogene;
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