• Journal of Microbiology and Biotechnology
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• v.22 no.12
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• pp.1621-1628
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• 2012

The agar-degrading bacterium GNUM-1 was isolated from the brown algal species Sargassum serratifolium, which was obtained from the West Sea of Korea, by using the selective artificial seawater agar plate. The cells were Gram-negative, $0.5-0.6{\mu}m$ wide and $2.0-2.5{\mu}m$ long curved rods with a single polar flagellum, forming nonpigmented, circular, smooth colonies. Cells grew at $20^{\circ}C-37^{\circ}C$, between pH 5.0 and 9.0, and at 1-10% (w/v) NaCl. The DNA G+C content of the GNUM-1 strain was 45.5 mol%. The 16S rRNA sequence of the GNUM-1 was very similar to those of Alteromonas stellipolaris LMG 21861 (99.86% sequence homology) and Alteromonas addita $R10SW13^T$(99.64% sequence homology), which led us to assign it to the genus Alteromonas. It showed positive activities for agarase, amylase, gelatinase, alkaline phosphatase, esterase (C8), lipase (C14), leucine arylamidase, valine arylamidase, ${\alpha}$-chymotrypsin, acid phosphatase, naphthol-AS-BI-phosphohydrolase, ${\alpha}$-galactosidase, ${\beta}$-galactosidase, ${\beta}$-glucosidase, catalase, and urease. It can utilize citrate, malic acid, and trisodium citrate. The major fatty acids were summed feature 3 (21.5%, comprising $C_{16:1}{\omega}7c/iso-C_{15:0}$ 2-OH) and C16:0 (15.04%). On the basis of the variations in many biochemical characteristics, GNUM-1 was considered as unique and thus was named Alteromonas sp. GNUM-1. It produced the highest agarase activity in modified ASW medium containing 0.4% sucrose, but lower activity in rich media despite superior growth, implying that agarase production is tightly regulated and repressed in a rich nutrient condition. The 30 kDa protein with agarase activity was identified by zymography, and this report serves as the very first account of such a protein in the genus Alteromonas.

• The Journal of the Korea Contents Association
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• v.14 no.3
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• pp.1-10
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• 2014

This study discusses crossmedia storytelling strategy plans by utilizing N-Screen service. It conceptualizes the definitions of N-Screen service in terms of smart contents. Related previous researches are extensively introduced to clarify how N-Screen service would be different from previous similar services. Most domestic N-Screen services have adopted OSMU method which provides the same contents from various platforms. It is, however, centered only on a development company's one-sided service and neglects to reflect the characteristics of a media or platform. In this light of gap, this study introduces ASMD method which is based on cross media. By providing right contents services according to types of smart media and enhancing a user's participation, users can enjoy a variety of entertainment and rich contents experiences and development companies can expect better promotion effects and an increase in sales. Finally, this study argues for strategies of crossmedia storytelling by using ASMD method, based on both domestic and overseas examples of contents development with crossmedia. As more and more diverse forms of media and platform would be introduced in the future, new strategies and perspectives would be imperative to cope with new environment. Conventional OSMU method bears inherent limitations to cover the range of N-Screen application. This study discusses the ways to mitigate the limitations by proposing ASMD method with crossmedia.

• Journal of Broadcast Engineering
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• v.24 no.6
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• pp.928-938
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• 2019

Republic of Korea has established 'Transmission and Reception for Terrestrial UHDTV Broadcasting Service' standard based on ATSC 3.0, the next-generation broadcasting standard in North America and is now providing commercial services. Republic of Korea has been studying terrestrial UHDTV based disaster broadcasting service using the ATSC 3.0 AEA system's technology since 2018. ATSC 3.0 has established a standard for expanding disaster broadcasting services, which were used to be simple push-type text message broadcasting, by introducing bidirectional and rich-media transporting mechanisms. However, the disaster information is still focused on the general public, and disaster broadcasting service including detailed information for the vulnerable are still insufficient. In this paper, we proposed the optimized disaster broadcasting service for vulnerable populations based on ATSC 3.0 after defined the disaster vulnerable populations as the target of the service. And we defined the extension element of disaster broadcasting message for service provision. The proposed service can be an effective means to increase the possibility of disaster information reception and evacuation to vulnerable populations. In addition, it is expected to be used as a basic research for development of diverse and effective advanced services for vulnerable populations through linkage with existing disaster alerting and countermeasures studies.

• The Journal of Korean Academy of Prosthodontics
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• v.42 no.2
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• pp.175-191
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• 2004

Statement of problem: Platelet-rich plasma(PRP) is well known to be very effective method to stimulate and accelerate the healing of bone and soft tissue. However, there are few reports which deal with the mechanisms of the PRP on the activation of the osteoblasts. Purpose: This study was aimed to investigate the effect of growth factors in PRP on the activity of osteoblasts. Material and method: To evaluate the effect on human, human osteoblast cell line was cultured. PRP was extracted from the blood of a healthy volunteer. Using the recombinant growth factors of PDGF, $TGFT-\beta$, IGF-1, bFGF which are mainly found at bone matrix and their neutralizing antibody, the effect of PRP on the attachment and proliferation of osteoblasts was evaluated. To evaluate the autocrine and paracrine effects, conditioned media(CM) of PRP was made and compared with PRP. By the western blot analysis, the expression of growth factors in PRP, CM was examined. Cell morphology was compared by the light microscope. Results : 1) The effects of CM on osteoblast were similar to the effects of PRP. 2) PRP, CM, recombinant $TGF-\beta$, bFGF, IGF-1 showed significantly higher cellular attachment than control(p<0.05) in the cell attachment assay. In the cell proliferation assay, PRP, CM, recombinant $TGF-\beta$, IGF-1, bFGF, PDGF increased significantly cell proliferation(p<0.01). Among the recombinant growth factors, IGF-1 showed the highest cellular attachment and proliferation. 3) In the western blot assay, bFGF, IGF-1, PDGF weve equally expressed in PRP and CM. 4) The attachment of osteoblast cell decreased significantly after the addition of neutralizing antibody against $TGF-\beta$, IGF-1(p<0.05). In the cell proliferation assay, the addition of neutralizing antibody against $TGF-\beta$, bFGF, PDGF, IGF-1 decreased significantly the cellular proliferation(p<0.05). The amount of decreasing in the cell attachment and proliferation is the highest in at-lGF-1. 5) The cells in control group were flattened and elongated with a few cellular processes in the a light microscope. But, the cells appeared as spherical, plump cells with well developed cellular processes in experimental groups. The cells in PRP and CM had more prominent developed features than recombinant growth factor groups. Conclusions : These findings imply that PRP maximize the cellular activity in early healing period using the synergistic effect, autocrine, paracrine effects of growth factors and increase the rate and degree of bone formation.

• Journal of Digital Contents Society
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• v.12 no.2
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• pp.241-252
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• 2011

During the last decades, other parts of operating systems, storage devices, and media are changed steadily, whereas filesystem is changed little. As data is grown bigger, the number of files to be managed also increases in geometrically. Researches about new filesystem schemes are being done widely to support these files efficiently. In web document search area, there are many researches about finding meaningful documents using semantic search. Many researches tried to apply these schemes, which is been proven in web document search previously, to filesystems. But they've focused only on higher layer of filesystem, that is not related seriously to storage media. Therefore they're not well tuned to physical characteristics of new flash memory based SSD which has different features against traditional HDD. We enhance log structured filesystem, that is already well known to work better in SSD, by putting semantic search scheme to and with multi logging point.

• Korean Journal of Microbiology
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• v.32 no.1
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• pp.70-77
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• 1994

The author isolated 7 mutant candidates which mapped around cysA (which was 10 o'clock). They were divided into two groups. One of them was located counterclockwise to cysA, and the other was clockwise to cysA. Since bldA was mapped counterclockwise to cysA, the candidate which mapped counterclockwise to cysA was transduced with phage containing wild type bldA gene clone. The candidates might be the alleles of bldA, because they were complemented by bldA clone. However some of such mutants sporulated very well and developed as much pigment as wild type on rich media plate. Their phenotype was not like bld mutant at all on such conditions. There were real antibiotics gene expressions, since transcriptional reporter gene xylE had shown high activities. Majority of the bldA like mutants showed act gene expressions when they were transformed with high copy number plasmid containing actII-ORF4.

• International Journal of Internet, Broadcasting and Communication
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• v.10 no.1
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• pp.71-76
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• 2018

The technology convergence of television, communication, and computing devices enables the rich social and entertaining experience through Smart TV in personal living space. The powerful smart TV computing platform allows to provide various user interaction interfaces like IR remote control, web based control, body gesture based control, etc. The presently used smart TV interaction user control methods are not efficient and user-friendly to access different type of media content and services and strongly required advanced way to control and access to the smart TV with easy user interface. This paper propose the optical wireless communication (OWC) based remote controller design for Smart TV using smart device Flashlights. In this approach, the user smart device act as a remote controller with touch based interactive smart device application and transfer the user control interface data to smart TV trough Flashlight using visible light communication method. The smart TV built-in camera follows the optical camera communication (OCC) principle to decode data and control smart TV user access functions according. This proposed method is not harmful as radio frequency (RF) radiation does it on human health and very simple to use as well user does need to any gesture moves to control the smart TV.

• Korean Journal of Environmental Agriculture
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• v.11 no.2
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• pp.116-124
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• 1992

This study was conducted to elucidate degradation pattern of two synthetic pyrethroid insecticides, bifenthrin having 2-methylbiphenyl group and cyhalothrin having ${\alpha}$-cyano benzyl ester group in theirs alcohol moiety, in two soils and aqueous media under laboratory conditions. The half-life of bifenthrin was 85.1 days and 12,4 days in Chilgok and Bokhyen soil of aerobic upland condition, respectively, and that of cyhalothrin was 54.6 days and 32.2 days. Bifenthrin and cyhalothrin were degraded very slowly under anaerobic flooded condition and sterilized. Their degradation seemed to be mainly mediated by aerobic microorganisms in soil. Bifenthrin and cyhalothrin were degraded more rapidly in Bokhyen soil with rich organic matter than Chilgok soil. Cyhalothrin was degraded 30 days faster than bifenthrin under aerobic upland condition of two soils. Cyhalothrin was degraded more than bifenthrin in alkaline solution of pH 10, but cyhalothrin and bifenthrin were degraded very slowly in acidic solution of pH 2 and 6.

• Applied Chemistry for Engineering
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• v.23 no.1
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• pp.100-104
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• 2012

Oligosaccharides production showed various biological activities in vivo like functional foods and industrial materials utilized available within many practical applications which have obtained from the degradation of alginate. Alginate is rich in the main component of seaweeds especially the brown algae. We investigated what degrading alginate from seaweeds to make alginate oligosaccharides can utilize in various fields using enzyme secreting Erwinia tasmaniensis. In this study, we observed an optimal culture condition of E. tasmaniensis, and characteristics of alginate lyase secreting E. tasmaniensis. These bacteria, E. tasmaniensis, were isolated from Nuruk. In this case, a suitable growth factor for E. tasmaniensis was culture it for 36 h in broth media on concentration of 1.0% (w/v) alginate. The enzyme showed the highest level of alginate lyase activity when cultured on broth media containing 1.0% (w/v) sodium alginate for 72 h. Optimal condition of pH, temperature and duration time for alginate lyase activity were found to be pH 6.0, $20^{\circ}C$ and 60 min, respectively.

• Smart Media Journal
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• v.9 no.2
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• pp.63-68
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• 2020

One of the most important parts of the user experience in mobile device interaction is navigation design. Navigation design plays a key role in enabling users to perform their desired functions and to access the information and content they need by mobile applications. In this process, differences in cultural characteristics and backgrounds of users may affect the navigation design, and thus the user experience may represent a significant difference. This study compared the navigation design of popular mobile shopping applications in China and the United States to examine the differences and analyzed the reasons. Based on this comparison, we proposed that the Chinese preferred rich information, colorful images and various menu styles, while Americans preferred simple designs, layouts and limited types of menu. In addition, two types of mobile shopping application navigation design schemes reflecting cultural characteristics were evaluated to examine the difference of Chinese and American users' preference, and the validity of our study was verified based on the results.