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Characterization of Erwinia tasmaniensis Isolated from Nuruk Producing Alginate Lyase  

Kim, Hyun Ji (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University)
Lee, Sung-Mok (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University)
Kim, Sung-Koo (Department of Biotechnology, Pukyong National University)
Lee, Jae-Hwa (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University)
Publication Information
Applied Chemistry for Engineering / v.23, no.1, 2012 , pp. 100-104 More about this Journal
Abstract
Oligosaccharides production showed various biological activities in vivo like functional foods and industrial materials utilized available within many practical applications which have obtained from the degradation of alginate. Alginate is rich in the main component of seaweeds especially the brown algae. We investigated what degrading alginate from seaweeds to make alginate oligosaccharides can utilize in various fields using enzyme secreting Erwinia tasmaniensis. In this study, we observed an optimal culture condition of E. tasmaniensis, and characteristics of alginate lyase secreting E. tasmaniensis. These bacteria, E. tasmaniensis, were isolated from Nuruk. In this case, a suitable growth factor for E. tasmaniensis was culture it for 36 h in broth media on concentration of 1.0% (w/v) alginate. The enzyme showed the highest level of alginate lyase activity when cultured on broth media containing 1.0% (w/v) sodium alginate for 72 h. Optimal condition of pH, temperature and duration time for alginate lyase activity were found to be pH 6.0, $20^{\circ}C$ and 60 min, respectively.
Keywords
alginate; alginate lyase; Erwinia tasmaniensis; oligosaccharide;
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1 D. S. Lee, H. R. Kim, D. M. Cho, T. J. Nam, and J. H. Pyeun, J. Kor. Fish. Soc., 31, 1 (1998).
2 J. I. Park, H. C. Woo, and J. H. Lee, Korean Chem. Eng. Res., 46, 833 (2008).
3 S.-M. Lee, J.-H. Kim, H. Y. Cho, H. Joo, and J. H. Lee, J. Korean Ind. Eng. Chem., 20, 517 (2009).
4 M. O. Yoon, S. C. Lee, J. W. Rhim, and J. M. Kim, J Korean Soc Food Sci Mutr., 33, 747 (2004).   DOI
5 D. S. Joo, S. Y. Cho, and E. H. Lee, Kor. J. Biotechnol. Bioeng., 13, 477 (1998).
6 B. Hu, Q. Gong, Y. Wang, Y. Ma, J. Li, and W. Yu, Anaerobe., 12, 260 (2006).   DOI
7 J. H. Lee, M. J. Bae, Y. C. Kim, and S. W. Nam, Kor. J. Microbiol. Biotechnol., 37, 350 (2009).
8 J. H. Lee and E. Y. Lee, Kor. J. Life Sci., 13, 718 (2003).   DOI
9 H.-M. Chen, L. Zheng, and X.-J. Yan, Food Technol. Biotechnol., 43, 29 (2005).
10 S. J. Horn, I. M. Aasen, and K. Ostgaard, J. Ind. Microbiol. Biotechnol., 25, 249 (2009).
11 S.-L. Kim, W.-J. Kim, S.-Y. Lee, and S. M Byun, J. Korean Agric. Chem. Soc., 27, 139 (1984).
12 S.-K. Paik, H.-S. Yun, K.-H. Sa, I.-S. Kim, I.-K. Rhee, H.-D. Park, C.-B. Yu, and I. Jin, Kor. J. Microbiol. Biotechnol., 31, 63 (2003)
13 J.-Y. Kong, New Informations of Oligosaccharides, 359, Yelim media, Seoul (2007)
14 M.-K. Jang, O. H. Lee, K. H. Yoo, D.-G. Lee, and S. H. Lee, J. life. Sci., 17, 1601 (2007).   DOI
15 J.-Y. Kong, S.-K. Bae, S.-H. Hwang, S.-D. Ha, H.-T. Kim, S.-K. Kim, and B.-J. Kim, Kor. J. Biotechnol. Bioeng., 11, 37 (1996).
16 T. Suzuki, K. Nagai, Y. Toshie, T. Shirai, and T. Hirono, Nippon Suisan Gakkaishi., 59, 879 (1933).
17 D. S. Lee, T. J. Nam, and J. H. Pyeun, J. Kor. Fish Soc., 31, 309 (1998).
18 I. H. Kim, D. S. Lee, J. Y. Kwon, M. T. Kwon, and T. J. Nam, J. Kor. Fish Soc., 36, 568 (2003).
19 S.-M. Lee and J.-H. Lee, Bioresour. Technol., 102, 5962 (2011).   DOI
20 O. J. Kim, D.-G. Lee, S.-M. Lee, S. J. Lee, H. J. Do, H. J. Park, A. Kim, J.-H. Lee, and J.-M. Ha, Kor. J. Microbiol. Biotechnol., 38, 144 (2010).
21 Y. Yonemoto, K. Mutata, A. Kimura, H. Yamaguchi, and K. Okayama, J. Ferm Bioeng., 72, 152 (1991).   DOI
22 M. H. Uo, D. S. Joo, and S. Y. Cho, J. Korean Soc. Food Sci. Nutr., 35, 109 (2006).   DOI
23 S. Joo, S. Y. Cho, and E. H. Lee, Kor. J. Appl. Microbiol. Biotechnol., 21, 207 (1993).
24 M. H. Uo, D. S. Joo, and S. Y. Cho, J. Korean Soc. Food Sci. Nutr., 35, 109 (2006).   DOI
25 H. K. Kim, J. C. Lee, N. H. Kang, S. H. Kim, J. G. Kim, and K. C. Chung, J. Life Sci., 17, 625 (2007).   DOI
26 Y.-O. Kim, G.-T. Kim, H.-K. Kim, D.-K. Kim, S.-H. Huh, and I.-S. Kim, J. Korean Fish. Soc., 29, 637 (1996).
27 D.-S. Joo, J.-S. Lee, J.-J. Park, S.-Y. Cho, C.-B. Ahn, and E.-H. Lee, Kor. J. Appl. Microbiol. Biotechnol., 23, 432 (1995).
28 D.-S. Joo, S.-Y. Cho, and E.-H. Lee, J. Korean Soc. Food Nutr., 22, 240 (1993).
29 T. Kobayashi, K. Uchimura, M. Miyazaki, Y. Nogi, and K. Horikoshi, Extremophiles, 13, 121 (2009).   DOI
30 M. H. Uo, D. S. Joo, S. Y. Cho, and T. S. Min, J. Korean Soc. Food Sci. Nutr., 35, 231 (2006).   DOI