• Research in Plant Disease
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• v.18 no.4
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• pp.387-390
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• 2012

Rice black-streaked dwarf virus (RBSDV) was reported to occur on maize plants in Gochang-gun of Jeonllabuk-do region in 2011. The symptoms typically include stunted and deformed leaves. Virus infected plants usually produce poor or no head. RT-PCR analysis of genomic dsRNA extracted from the plant confirmed the infection. Specific primers for full length genome of segments 8 and 10 were used for RNA amplification. Full-length genomes of S8 and S10 were cloned and sequenced. Sequence analysis revealed that the S8 and S10 sequences of the maize isolate were same with rice isolate in size, 1,936 nt and 1,801 nt, respectively. In comparison with rice RBSDV, S8 and S10 showed 94.9-99.6% and 94.1-98.4% sequence identity, respectively. Phylogenetic analysis showed that RBSDV S8 and S10 of maize plants are categorized into the same group as RBSDV of rice plant.

• Korean Journal Plant Pathology
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• v.1 no.2
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• pp.109-114
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• 1985

The experiments were conducted to clarify the influence of transplanting date on the occurrence of rice virus in field condition of 1984. The rate of RBSDV (rice black-slreaked dwarf virus) viruliferous vector, smaller brown planthopper (Laodelphax striatellus Fallen), was shown to be $13.6\%$ at the 2nd adult and that of rice stripe virus (RSV) viruliferous was $6.7\%$ at the 2nd adult. The vector in the field was begun to come from May 29, the maximum densities were 19.6 insects per hill on June 13 in cultivar Chucheongbyeo, 19.3 in Nagdongbyeo, 7.4 in Cheongcheongbyeo and 4.9 in Samgangoyes. The number of vectors per hill was inclined to increase by early transplanting. Although the infection of rice virus in nursery bed was not recognized until May 30 transplanting, the nursery infection could be seen from June 10 transplanting. The highest rate of nursery infection with RSV was $4.1\%$ at June 10 transplanting plot, and that of RBSDV was $14.2\%$at June 20 trans planting plot. The infection of rice virus in paddy field was the highest at May 20 transplanting plot, the lowest at July 10 plot. The earlier transplanting, the more severe occurrence of rice viruses. Occurrence of infected plants with RBSDV was shown to increase more rapidly at May 20 and May 30 planting plot than May 10 plots. However, the occurrence of infected plant with RSV was more rapid at May 10 transplanting plot than May 20 and 30.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.1
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• pp.74-80
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• 1988

Seasonal population changes in Laodelphax striatellus Fallen (small brown planthopper), percentage of rice black-streaked dwarf virus (RBSDV) viruliferous L. striatellus, and RBSDV infection of 11 corn hybrids were observed at various locations and years. The population size of L. striatellus was relatively small in the middle parts of Korea, but it was much greater in the southern areas. The population size of the first generation of adult L. striatellus emerged from late April to early May was similar or smaller compared with that of the second generation emerged in middle June in the middle parts of Korea. However, in the southern areas the population size of second generation was much greater than the first generation. The percentage of RBSDV viruliferous L. striatellus differed depending on the years, locations, and testing methods. The percentage of viruliferous vector was highest in southern plain areas and it tended to decrease with distance from the areas. The percentage of viruliferous vectors tested by enzyme-linked immunosorbent assay was higher than that tested by rice seedling test. The RBSDV infection rate of corn hybrids was highest at Daegu and ranged from 9 to 39% probably due to both a higher L. striatellus population and a higher percentage of viruliferous vectors. However, it was significantly lower in other areas and ranged from 0 to 13%.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.3
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• pp.221-228
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• 2008

This experiment was carried out to investigate the actual outbreak status of rice black-streaked dwarf virus (RBSDV) disease in forage corn for securing the basic data to control of the disease all over the country from 2006 to 2007. Outbreak of the disease showed a wide difference according to regions and was severe in Icheon, Gochang, Kimje, Youngkwang, Kimchun, Youngju, Cheonan, and Boroung provinces in Korea. Outbreak rate of the disease was effected by planting time of forage corn. Disease rate was lower in corns planted from Apr. 26 to May 15 than in those of planted from April 10 to 25 or after May 31. The corn fields planted from April 26 to May 15 showed lower disease rate than that of planted from April 10 to 25 or after May 31. Also the outbreak rate of the disease was some difference according to corn varieties but was not genuinely resistant varieties to RBSDV. Dry matter yield of forage corn was significantly decreased depending on disease rate, and was highly negative correlation to disease rate. The forage corn infected with RBSDV was lower 3.2%, 3.6% and 12% in IVDMD, TDN, and RFV, and higher 3.7% and 4.5% in NDF and ADF than those of the healthy plant, respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.3
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• pp.229-235
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• 1988

Several agronomic characters, feed value, and mineral uptake of two corn hybrids infected by different degrees of rice black-streaked dwarf virus (RBSDV) were studied at the south-eastern part of RBSDV prevalent areas in Korea. Although both hybrids were infected with RBSDV by 100%. culm length of Suweon 19 was more severely reduced than that of Jinjuok and there were positive correlations between culm length and top dry weight of individual plants. The reduced culm length was largely due to the shortened length of upper five internodes in both hybrids and also due to the reduced number of internodes in Jinjuok. As culm length reduced, percent ear-bearing plants, average ear weight, and number of kernels per ear reduced significantly in both hybrids. The kernel weight of Suweon 19 decreased with reduced culm length, but that of Jinjuok was similar among the different culm lengths. However, as culm length decreased, crude protein and fiber in whole plants and N, P, K, Mg in culm ＋ leaf sheath increased, but nitrogen free extract in whole plant decreased. And crude fat in whole plant and N, P, K, Mg concentrations in leaf blades and ear were similar among the culm lengths.

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.4
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• pp.323-334
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• 2004

Silage yield Potential and some agronomic characteristics of Korean improved and introduced corn hybrids from the United States were tested for five year in Gyeongsan, Gyeongsangbug-do and for one year in Hongcheon, Gangwon-do. 1. At 20 days after emergence, plant height and dry matter of hybrids were different, while the early growth of the hybrids was not correlated to the culm length and silage yield. 2. In Cyeongsan, silage yield potential of Suwon19 was relatively high, while most susceptible to rice black-streaked dwarf virus (RBSDV) disease and leaf senescence at harvest time. In contrast, silage yield potential and resistance to RBSDV of Cwanganok were moderate, while susceptible to leaf senescence at harvest. Generally, Suwon19 showed similar or higher yield than most introduced hybrids, while some introduced hybrids showed lower silage yield than Korean improved hybrids. Most introduced hybrids were more resistant to RBSDV and leaf senescence at harvest time compared to Korean improved hybrids. 3. In Hongcheon, silage yield of three Korean improved hybrids were lower than that of NC+5514 and DK729, while similar or higher than other introduced hybrids. af senescence of all Korean hybrids was severer compared to introduced hybrids.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.2
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• pp.111-116
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• 2013

This experiment was carried out to select a resistant corn variety for rice black-streaked dwarf virus (RBSDV) disease in a RBSDV prevalent area (Gochang of Jeollabukdo) from 2006 to 2008. Ten corn varieties for silage preparation were cultivated with first cropping and second cropping system in an RBSDV field, and were tested outbreak rates of RBSDV and dry matter (DM) yield of forage. The outbreak rates of RBSDV were significantly different between corn varieties. Therefore, the resistance degree of corn varieties for RBSDV were divided into 4 groups; very resistant ('Kwangpeyongok' and 'Kwanganok'), resistant ('P3156' and 'P3394'), mildly resistant ('Cheonganok' and 'P32P75') and susceptible ('Suwon19', 'DK697' and 'GW6959') groups respectively. DM yield of forage in RBSDV fields exhibited comparatively significant differences between varieties (p<0.05). DM yield of resistant varieties ('Kwangpeyongok', 'Kwanganok', 'P3156' and 'P3394'), were higher (14~26%) than those of susceptible varieties ('Suwon 19', 'DK697' and 'GW6959'). Therefore, resistant varieties were recommended for increasing forage productivity in field of RBSDV prevalent areas.

• Journal of The Korean Society of Grassland and Forage Science
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• v.37 no.1
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• pp.44-49
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• 2017

This experiment was carried out to study the effect of rice black-streaked dwarf virus(RBSDV) infection rate on forage productivity of corn varieties in Cheonan of chungcheongnamdo from 2006 to 2008. Forage corn varieties of 10 were cultivated with first cropping(seeding in the last ten days of April) and second cropping(seeding in the last ten days of May) system in field and tested the infection rates of RBSDV and productivity of forage. The Infection rate of RBSDV was significant difference between corn varieties in middle district of Korea. Resistant corn varieties for RBSDV were 'Kwanganok', 'P3156', 'Kwangpeyongok' and 'P3394' but susceptible varieties were 'Suwon19', 'DK697', 'GW6959' and NC+7117. Dry matter(DM) yield of forage corn according with infection rates of RBSDV in field was significant difference between varieties(p<0.05). DM yield of susceptible varieties, 'Suwon 19', 'DK697' and 'GW6959' was lower about 20% than that of resistant varieties, 'Kwangpeyongok' and 'P3156'. For increasing the productivity of forage corn, recommend of resistant varieties for RBSDV and control of seeding time are very important in middle district of Korea.

• Research in Plant Disease
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• v.15 no.2
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• pp.57-62
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• 2009

Genetic diagnosis method of Virion Captured (VC)/RT-PCR for Rice stripe virus (RSV) and Rice black-streaked dwarf virus (RBSDV), Korean major rice viruses transmitted by small brown plant hopper, Laodelphax striatellus, was developed. Virion extraction buffer for rice plant was 0.01M potassium phosphate buffer, pH 7.0, containing 0.5% sodium sulfite. However, the extraction buffer for L. striatellus was 0.01M potassium phosphate buffer, pH 7.0, containing 0.5% sodium sulfite and 2% polyvinylpyrrolidone wt 40,000 (PVP-40). Specific primers for detection of RSV and RBSDV were selected for VC/RT-PCR method. The specific primers were used as a duplex primer to detect viruliferous small brown plant hopper collected from Gimpo, Pyeongtaek and Siheung areas in Gyeonggi province. The genetic diagnosis methods of single and duplex VC/RT-PCR for RSV and RBSDV could be used easily and economically, especially on the diagnosis of L. striatellus. The rate of viruliferous insect (RVI) for RSV was compared with ELISA and VC/RT-PCR for L. striatellus collected from fields. RVI by ELISA was same as 9.2% with RVI by VC/RT-PCR. However, there were some different detection results between the methods. It could be suggested that there is a possibility of serological and/or genomic differences among RSV isolates. The portion of RVI detected simultaneously by ELISA and VC/RT-PCR was 71.0%, and the detection rate from VC/RT-PCR was higher as 3.2% than that from ELISA, which had a reason of simultaneous detection ability both RSV and RBSDV of VC/RT-PCR.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.3
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• pp.161-172
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• 1992

The agronomic performance of four introduced corn(Zea mays L.) hybrids and a domestic hybrid Suwon 19(control) was compared for silage production in the Middle Northwestern Coast(MNWC). Middle Southwestern Coast(MSWC) and Middle Northern Inland(MN1) regions of Korea, 1988 to 19 90. In the MNWC region, DK713 and P3144W were recorded as more resistant corn hybrids to rice black streaked dwarf virus(RBSDV) than other hybrids. P3144W, P3352 and DK689 hybrids produced higher dry matter(DM) and total digestible nutrient(TDN) than the control hybrid Suwon 19 and DK 713. In the MSWC region, P3144W and DK689 tended to yield more DM and TDN than other hybrids and a severe infection of RBSDV was observed during 3 years of experiment. In the MNI region, P3144W and P3352 corn hybrids showed higher ear and dry matter percentage and gave the highest DM and TDN yield compared with other hybrids. The RBSDV infection of the hybrids in the MNI region was moderate level compared with the two regions, but rate of stay green was the lowest. In the three regions, the control hybrid Suwon 19 gave the highest RBSDV infection, followed by P 3352, DK689, and P3144W, while DK713 was the lowest. P3144W and P3352 hybrids gave higher DM and ear percentages than all other hybrids at harvest. During 3 years of experiment, it was found that there were regional and year differences in RBSDV infection, forage DM yield, DM percentage and ear ratio to total DM yield of the corn hybrids.