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http://dx.doi.org/10.5423/RPD.2012.18.4.387

Sequence Analysis of Segments 8 and 10 of Rice black-streaked dwarf virus from Maize Plants  

Lee, Bong Choon (Crop Environment Research Division, National Institute of Crop Science, Rural Development Administration)
Cho, Sang-Yun (Crop Environment Research Division, National Institute of Crop Science, Rural Development Administration)
Yoon, Young-Nam (Functional Crop Resource Development Division, National Institute of Crop Science, Rural Development Administration)
Kang, In Jeong (Crop Environment Research Division, National Institute of Crop Science, Rural Development Administration)
Kwak, Do Yeon (Functional Crop Resource Development Division, National Institute of Crop Science, Rural Development Administration)
Shin, Dong Bum (Crop Environment Research Division, National Institute of Crop Science, Rural Development Administration)
Kang, Hang-Won (Crop Environment Research Division, National Institute of Crop Science, Rural Development Administration)
Publication Information
Research in Plant Disease / v.18, no.4, 2012 , pp. 387-390 More about this Journal
Abstract
Rice black-streaked dwarf virus (RBSDV) was reported to occur on maize plants in Gochang-gun of Jeonllabuk-do region in 2011. The symptoms typically include stunted and deformed leaves. Virus infected plants usually produce poor or no head. RT-PCR analysis of genomic dsRNA extracted from the plant confirmed the infection. Specific primers for full length genome of segments 8 and 10 were used for RNA amplification. Full-length genomes of S8 and S10 were cloned and sequenced. Sequence analysis revealed that the S8 and S10 sequences of the maize isolate were same with rice isolate in size, 1,936 nt and 1,801 nt, respectively. In comparison with rice RBSDV, S8 and S10 showed 94.9-99.6% and 94.1-98.4% sequence identity, respectively. Phylogenetic analysis showed that RBSDV S8 and S10 of maize plants are categorized into the same group as RBSDV of rice plant.
Keywords
DsRNA; RBSDV; Sequence;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Choi, H. S., Lee, S. H., Kim, M. K., Kwak, H. R., Kim, J. S., Choi, J. D. and Choi, G. S. 2010. Occurrence of virus diseases on major crops in 2009. Res. Plant. Dis. 16: 1-9. (In Korean)   과학기술학회마을   DOI
2 Lee, B. C., Hong, Y. K., Hong, S. J. and Park, S. T. 2006. Occurrence of Rice black-streaked dwarf fijivirus in maize. Res. Plant Dis. 12: 62-64. (In Korean)   과학기술학회마을   DOI
3 Lee, B. C., Hong, Y. K. Hong, S. J., Park, S. T. and Lee, K. W. 2005. Occurrence and detection of Rice black-streaked dwarf virus in Korea. Plant Pathology J. 21: 172-173. (In Korean)   과학기술학회마을   DOI
4 Lee, M. H., Hey, C. H., Park, K. B. and Lee, Y. S. 1989. Productivity of silage by chemical control under different planting date in the Rice black-streaked dwarf virus of corn. Res. Rept. RDA 31: 31-35. (In Korean)
5 Fang, S., Yu, J., Feng, J., Han, C., Li, D. and Liu, Y. 2001. Identification of Rice black-streaked dwarf fijivirus in maize with rough dwarf disease in China. Arch Virol. 146: 167-170.   DOI   ScienceOn
6 Isogai, M., Uyeda, I. and Choi, J. K. 2001. Molecular Diagnosis of Rice black-streaked dwarf virus in Japan and Korea. Plant Pathology J. 17: 164-168.
7 Shikata, E. and Kitagawa, Y. 1977. Rice black-streaked dwarf virus: Its properties, morphology, and intracellular localization. Virology 77: 826-842.   DOI   ScienceOn
8 Uyeda, I., Lee, B. C. and Ando, Y. 1998. Reovirus isolation and RNA extraction. Meth. Mol. Biol. 81: 65-75.
9 Wang, Z. H., Fang, S. G., Xu, J. L. Sun, L. Y., Li, D. W. and Yu, J. L. 2003. Sequence analysis of the complete genome of Rice black-streaked dwarf virus isolated from maize with rough dwarf disease. Virus Genes 27: 163-168.   DOI   ScienceOn
10 Zhang, H. M., Chen, J. P. and Adams, M. J. 2001. Molecular characterization of segments 1 to 6 of Rice black-streaked dwarf virus from China, provides the complete genome. Arch Virol. 146: 2331-2339.   DOI   ScienceOn