식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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옥수수에 발생한 벼검은줄오갈병 바이러스 분절게놈 S8 및 S10 전 염기서열 분석

Sequence Analysis of Segments 8 and 10 of Rice black-streaked dwarf virus from Maize Plants

  • 이봉춘 (국립식량과학원 작물환경과) ;
  • 조상윤 (국립식량과학원 작물환경과) ;
  • 윤영남 (국립식량과학원 기능성작물부) ;
  • 강인정 (국립식량과학원 작물환경과) ;
  • 곽도연 (국립식량과학원 기능성작물부) ;
  • 신동범 (국립식량과학원 작물환경과) ;
  • 강항원 (국립식량과학원 작물환경과)
  • Lee, Bong Choon (Crop Environment Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Cho, Sang-Yun (Crop Environment Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Yoon, Young-Nam (Functional Crop Resource Development Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kang, In Jeong (Crop Environment Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kwak, Do Yeon (Functional Crop Resource Development Division, National Institute of Crop Science, Rural Development Administration) ;
  • Shin, Dong Bum (Crop Environment Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kang, Hang-Won (Crop Environment Research Division, National Institute of Crop Science, Rural Development Administration)
  • 투고 : 2012.11.05
  • 심사 : 2012.11.16
  • 발행 : 2012.12.31
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초록

2011년에 고창 옥수수 재배지에서 RBSDV에 의한 벼 검은줄오갈병이 발생하였다. 병징은 키가 작아지고 잎에 줄무늬가 형성되면서 심하면 거의 수확이 되지 않는다. 이병주로부터 직접 dsRNA를 추출하여 RT-PCR에 의해 감염을 확인하였다. S8 및 S10 분절의 전 게놈에 특이적인 primer를 이용하여 유전자를 증폭하였다. 이것을 크로닝한 후 전 염기서열을 결정하였다. 염기서열 분석결과 S8 및 S10의 전염기서열은 각각 1,936 nt 및 1,801 nt로 구성되었다. 이는 벼에서 분리된 RBSDV와 같은 크기로 확인되었다. 벼에서 분리된 RBSDV와 상동성 분석결과 S8은 94.9-99.6%, S10은 94.1-98.4%로 나타났다. Phylogenetic tree 분석결과 옥수수에서 분리한 RBSDV S8 및 S10 모두 벼에서 분리된 RBSDV와 같은 그룹으로 분류되었다.

Rice black-streaked dwarf virus (RBSDV) was reported to occur on maize plants in Gochang-gun of Jeonllabuk-do region in 2011. The symptoms typically include stunted and deformed leaves. Virus infected plants usually produce poor or no head. RT-PCR analysis of genomic dsRNA extracted from the plant confirmed the infection. Specific primers for full length genome of segments 8 and 10 were used for RNA amplification. Full-length genomes of S8 and S10 were cloned and sequenced. Sequence analysis revealed that the S8 and S10 sequences of the maize isolate were same with rice isolate in size, 1,936 nt and 1,801 nt, respectively. In comparison with rice RBSDV, S8 and S10 showed 94.9-99.6% and 94.1-98.4% sequence identity, respectively. Phylogenetic analysis showed that RBSDV S8 and S10 of maize plants are categorized into the same group as RBSDV of rice plant.

키워드

참고문헌

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