• Journal of Applied Biological Chemistry
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• v.63 no.2
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• pp.147-152
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• 2020

Farrerol is a flavanone isolated from the traditional Chinese herb 'Man-shan-hong' (Rhododendron dauricum L.). Farrerol has been reported to have various bioactivities including anti-oxidant, anti-inflammation, and anti-fungal. However, anti-cancer effect of farrerol has not yet been reported in MCF-7 breast cancer cells. In the present study, we investigated the anti-cancer effect of farrerol on MCF-7 cells. Farrerol decreased viability and induced apoptosis of MCF-7 cells in a dose dependent manner. Ferrerol exhibited a significant anti-proliferation effect with a half-maximal inhibitory concentration (IC₅₀) values of 145.04±1.4 μM in MTT assay, when MCF-7 cells were treated with ferrerol for 48 h. Also, ferrerol induced apoptotic bodies of MCF-7 cells as evaluated by TUNEL assay and Annexin V/PI staining using FACS. By mechanism of action, ferrerol regulated the activation of p38 mitogen-activated protein kinase and altered the expression level of BAX, Bcl-2, and Poly ADP Ribose Polymerase in MCF-7 cells. In summary, our finding demonstrated that ferrerol has anti-cancer effect through regulating the activation and expression of apoptosis-related proteins in MCF-7 cells.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.2 no.3
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• pp.18-24
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• 1999

This study was aimed to develope appropriate soil media for the growth of Rhododendron hybrid $J_{ASANHONG}$ on the artificial ground five types of soil media was tested such as "sandy loam-general soil (T1)", "vermiculite-artificial soil (T5)", "sandy loam 50% + vermiculite 30% + sand 20% (improved of soil 2-T2)", "sandy loam 50% + carbonized rice hust 30% + sand 20% (improved of soil 3-T3)", "sandy loam 50% + humus sawdust 30% + sand 20% (improved of soil 4-T4)". The result of the research are as follows. 1. Among the type of soil media, the sandy loam(T1) soil type gave the worst effects on growth of above ground parts(height, No. of leaf, width of leaf, No. of flowering, dry weight of upper parts) and under ground parts(dry weight of roots). 2. Vermiculite(T5) showed the highest root growth(dry weight of roots). it seemed to be caused high saturated hydraulic conductivity and porosity. As a result, there is much available space for enabling the root spreads. 3. "sandy loam 50% + vermiculite 30% + sand 20%(improved of soil 2-T2)", "sandy loam 50% + carbonized rice hust 30% + sand 20%(improved of soil 3-T3)" showed good effects on growth of above ground parts and under ground parts compared with sandy 10am(T1) 4. "sandy loam 50% + humus sawdust 30% + sand 20% + (improved of soil 4-T4)" showed the highest effects on growth of above ground parts.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.8 no.6
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• pp.69-79
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• 2005

This study was carried out to select proper species for early stage replantation in highway cut-slope area. In highway cut-slope area, sample plots of 106 were selected, and their vegetations and environmental factors were investigated. 1. We found total 172 species in the 106 cutting area of highway. The species of high frequency of highway cut-slope were found in the order of Lespedeza bicolor, Artemisia princeps var. orientalis, Festuca arundinacea, Erigeron annuus, Lespedeza cuneata, Lactuca indica var. laciniata, Eragrostis curvula, Dactylis glomerata, Oenothera lamarckiana, Wistaria floribunda, Humulus japonica, Commelina communis, Miscanthus sinensis, Pueraria thunbergiana, Pinus densiflora, etc. 2. The average vegetation coverage was over 90% in the study sites and the average coverage was 91.4% in the total cut-slope area. The species of high coverage of highway cut-slope area were found in the order of Festuca arundinacea, Eragrostis curvula, Lespedeza bicolor, Wistaria floribunda, Lespedeza cuneata, Dactylis glomerata, Artemisia princeps var. orientalis, Humulus japonica, Pueraria thunbergiana, Robinia pseudoacacia, Poa pratensis, Medicago sativa, Festuca ovina, Pinus densiflora, Parthenocissua tricuspidata, etc. 3. The total coverage in the foreign plants of Festuca arundinacea, Eragrostis curvula, Dactylis glomerata, Poa pratensis, Medicago sativa, Coreopsis drummondii and native plants of Lespedeza bicolor, Wistaria floribunda, Lespedeza cuneata, Amorpha fruticosa, Indigofera pseudotinctoria, Lespedeza cyrtobotrya were 57.52%. That is, the ecological succession of native herbs and parachute shrubs have delayed because the afforested plants occupy 57.52%. In future, the coverage of foreign herbs have to reduce, and the coverage of the native herbs and parachute shrubs must be increased. 4. The native seed of Artemisia sp., Miscanthus sinensis, Smilax china, Pueraria thunbergiana, Rubus crataegifolius, Rubus parvifolius, Pinus densiflora, Rhus chinensis, Albizzia julibrissin, Rhododendron mucronulatum, Clematis apiifolia, Zanthoxylum schinifolium, Prunus sargentii could be added in the seedling of the temperate south zone highway with the used seeds. The native seed of Artemisia sp., Miscanthus sinensis, Rubus crataegifolius, Rhododendron mucronulatum, Weigela subsessilis, Stephanandra incisa, Rhus chinensis, Pinus densiflora, Salix koreensis, Cocculus trilobus, Populus alba, Spiraea prunifolia for. simpliciflora, Clematis apiifolia, Lindera obtusiloba, Quercus serrata, etc., could be added in the seedling of the temperate middle zone highway with the used seeds. 5. We have some recommendation. The native plants have to growth in the highway cut-slope area instead of foreign plants to have good environmental ecology. The role of the foreign plants should be the plant for the initial several years in the highway cut-slope area. And, the native plants should growth in the next season. 6. We should protect shrubs and trees in the highway slope area because shrubs and trees can be more helpful in stabilizing of the slope area than herbs.

• Korean Journal of Environment and Ecology
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• v.29 no.3
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• pp.441-453
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• 2015

In this paper, according to the illuminance at night with artificial lighting in downtown Seoul, trees flowering and leaf growth rate was performed as a case study to examine whether there is a difference. The illuminance was measured at 78 points on a total of 26 points, the range of illuminance were divided into three groups considering land use, Group A (plots 1-7) were 4.90 ~ 112.50 lx, Group B (plots 8 to 18) were 0.45 ~ 42.40 lx, group C (plots 19 to 26) were 0.28 ~ 22.10 lx. According to the One-way ANOVA on illuminance difference, the survey groups were classified into three groups. To survey the ratio of the flowering Prunus yedoensis and Rhododendron spp. and to survey the ratio of leaf growth Zelkova serrata, Ginkgo biloba, and Acer palmatum each 30 individuals were investigated. Rhododendron spp. to study the ratio of the flowering and Acer palmatum to study the ratio of leaf growth were determined to be useful as indicator woody species according to the correlation. The higher illuminance of night lighting is, the faster flowering and leaf growth of trees will be by correlation between the mean illuminance of each group and the flowering and leaf growth of trees. In the future, should be made a more detailed study of how much affect to the trees with some impact on the level of light pollution at night lighting.

• Korean Journal of Environment and Ecology
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• v.32 no.2
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• pp.185-202
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• 2018

Many ecological studies regarding forest succession, which is the process of arriving at climax forest through ecological changes, have suggested that Carpinus laxiflora is the leading tree type of the climax forest of temperate zone in South Korea. However, most of these studies on C. laxiflora forest have focused on vegetation structure and succession process in specific areas or regional physiognomical forest communities, and thus some may question whether it is rational to consider C. laxiflora forest as the climax forest in South Korea's temperate forest zone. The purpose of this study was to analyze the vegetation classification, species composition, and stratum structure and to investigate the succession tendency with the vegetation data collected from C. laxiflora forests in 75 quadrates in 17 mountains between 2014 and 2017. We used the phytosociological method to analyze the vegetation structure and the importance value to analyze the stratum structure. The results showed the floristic composition of 6 vegetation units and 9 species groups. The hierarchy of the C. laxiflora community group was represented by the Ulmus laciniata community, Corylus heterophylla community (subdivided Quercus aliena group and Corylus heterophylla typical group), and Rhododendron schlippenbachii community (subdivided Fraxinus chiisanensis group, Carpinus tschonoskii group, and Rhododendron schlippenbachii typical group). Successional tendency of C. laxiflora forest based on the importance of each layer is predicted to be the total of three types such as to maintain the stand (vegetation units 2, 3, and 6), to success the Carpinus cordata forest (vegetation units 1 and 4), and to success Quercus acuta forest (vegetation unit 5).

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.3
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• pp.427-432
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• 2005

This study was performed to investigate the effect of natural plant extracts on acetylcholinesterase (AChE) activity and the free radical scavenging activity. The methanolic extracts of plants were tested for AChE inhibitory activity using Ellman's colorimetric method in 96-welled microplates and antioxidant activity as the scavenging effect of 1, 1-diphenyl-2-picryl hydrazyl radical (DPPH). The results showed that AChE activities were inhibited (about 20-30%) in whole plant extract of Daucus carota var. sativa, Hypericum erectum and Fragaria yezoensis. AChE activities were inhibited (about 32-34%) in stems extract of Gingko biloba and leaves extract of Rhododendrondron yedoensa var. poukhanense. Fruit extract of Zanthoxylum schinifolium inhibited (about 18%) AChE activity. And the DPPH scavenging effects as antioxidant activity were similar to L-ascorbic acid in whole plant extract of Fragaria yezoensis and fruits extract of Comus officinalis.

• Korean Journal of Environment and Ecology
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• v.3 no.1
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• pp.42-50
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• 1989

A valley and a ridge forest in Mt. Gaya area was studied to investigate forest structure in relation to altitude and part of slope. Sixty-three quadrats were set up in the valley forest along altitude of 600m to 1,000m and part of slope, and thirty-eight quadrats were set up in the ridge forest along altitude of 700m to 1,430m. According to the importance values, the valley forest was Quercus mongolica-Lespedeza maximowiczii community and the ridge forest was Pinus densiflora, Quercus mongolica-Rhododendron mucronulatum community. Similarity index between the valley forest community and the ridge forest community was 37.2%. Shannon's species diversities of the valley forest community and the ridge forest community were 1.3402 and 1.0098, respectively. According to importance values by crown stories and DCA ordination, successional trends of tree species may be from Pinus densiflora and Pinus koraiensis through Quercus mongolica to Quercus serrata and Carpinus laxiflora. As going from the lower part to upper part of the slope in the valley forest, the importance values of Quercus mongolica, Quercus aliena, Rhododendron mucronulatum and Lespedeza maximowiczii increased while those of Carpinus laxiflora and Fraxinus rhynchophylla decreased. With increasing elevation in the valley and ridge forest, the importance value of Pinus densiflora decreased while that of Quercus mongolica increased. In the valley forest, densities of canopy and shrubstratum increased as increasing elevation, and the number of species and species diversity decreased as increasing elevation and going from the lower part to the upper of slope. The range of similarity indices between parts of the slope, and the elevation belts of 100m in the valley forest were 66.6-69.2 and 25.9-79.8%, respectively. In the ridge forest, density and basal area of canopy tended to decreased as increasing elevation, and the range of similarity indices between elevation belts of 100m was 27.9-98.2%.

• Korean Journal of Environment and Ecology
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• v.20 no.2
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• pp.122-129
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• 2006

To investigate the vegetation structure of mountain region from Wolgagsan to Dogabjae, 14 plots($2000m^2$) set up with random sampling method were surveyed. Two groups of Quercus variabilis-Pinus densiflora community, Quercus mongolica-Quercus acutissima community were classified by cluster analysis. Quercus variabilis, Pinus densiflora, Quercus mongolica, Quercus acutissima, Quercus dentata and Quercus serrata were found as a major woody plant species in Weolchulsan National Park region. In this area, Quercus variabilis and Pinus densiflora were dominated partially. In the future, the importance percentage of Pinus densiflora might be decreased, but those of Quercus variabilis, Quercus mongolica, Quercus acutissima and Quercus dentata might be increased. High positive correlations was proved between Quercus variabilis and Pinus rigida; Styrax japonica and Quercus dentata, Quercus acutissima; Pinus rigida and Lindera erythrocarpa, Pinus densiflora; Lindera obtusiloba and Rhododendron mucronulatum; Albizzia julibrissin and Styrax obassia, and relatively high negative correlations was proved between Diospyros kaki and Quercus dentata; Platycarya strobilacea and Lindera erythrocarpa; Symplocos chinensis for. pilosa and Quercus acutissima; Styrax japonica and Styrax obassia; Pinus rigida and Lindera obtusiloba, Fraxinus sieboldiana, Rhododendron mucronulatum; Quercus mongolica and Albizzia julibrissin. Species diversity(H') of investigated groups was ranged $1.2430{\sim}1.2892$.

• Korean Journal of Environment and Ecology
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• v.21 no.3
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• pp.257-277
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• 2007

We carried out a phytosociological study on pine forests in Korea with the method of Zurich-Montpellier School. We collected the data of 252 $relev{\acute{e}}s$ from 45 sites in the pine forests throughout the Korean Peninsula and its attached islands. The vegetation of the pine forests was classified into one association, three communities and seven subcommunities as follows: A: Quercus mongolica-Pinus densiflora community, A-1: Typical subcommunity, A-2: Vaccinium korean urn subcommunity, A-3 : Rhododendron micranthum subcommunity, B: Quercus serrata-Pinus densiflora community, B-1: Typical subcommunity, B-2: Juniperus rigida subcommunity, B-3: Styrax japonica subcommunity, B-4: Eurya japonica subcommunity, C: Saso-Pinetum densiflorae Yim et al. 1990, and D: Castanopsis cuspidata var. sieboldii-Pinus densiflora community. The former three communities were integrated into the Lindero-Quercion mongolicae Kim 1990 em. 1992. The Castanopsis cuspidata var. sieboldii-Pinus densiflora community remained to be studied in future to determine the association. The communities of Quercus mongolica-Pinus densiflora community was distributed throughout the montane zone in central-northern part of the Korean Peninsula. Quercus serrata-Pinus densiflora community occupied widely in the sub-montane and hilly areas in central and Southern Korean Peninsula. The association of Saso-Pinetum densiflorae was found in Cheju Island. Castanopsis cuspidata var. sieboldii-Pinus densiflora community were distributed in the warm-temperate zone including islands off the south-west coast of the Peninsula.

• Korean Journal of Environment and Ecology
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• v.28 no.6
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• pp.741-750
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• 2014

To investigate the vegetation structure of mountain ridge from Guryongryeong to Mt. Yaksu, 22 plots ($100m^2$) installed with random sampling method were surveyed. Three groups of Quercus mongolica-Acer pseudosieboldianum community, Q. mongolica community, Cornus controversa-Q. mongolica community were classified by cluster analysis. Q. mongolica was a major woody plant species in the ridge area from Guryongryeong to Yaksusan and Carpinus cordata and C. controversa was partly occupied in some area. High positive correlations showed between Q. mongolica and Symplocos chinensis for. pilosa, Rhododendron schlippenbachii; Tilia amurensis and Tilia mandshurica, Symplocos chinensis for. pilosa; Tilia mandshurica and S. chinensis for. pilosa, R. schlippenbachii; Betula costata and Acer mono; Symplocos chinensis for. pilosa and Rhododendron schlippenbachii, and relatively high negative correlations showed between A. pseudosieboldianum and S. chinensis for. pilosa, R. schlippenbachii. Species diversity(H') of investigated groups were ranged 0.8170~1.1446 and it was lower compared to those of the ridge area of the national parks in Baekdudaegan.