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http://dx.doi.org/10.3839/jabc.2020.020

Anti-cancer effect of farrerol induced apoptosis through activating p38 MAPK in Human breast cancer MCF-7 cells  

Chae, Jongbeom (Department of Food Science and Biotechnology, Kyungpook National University)
Lee, Seul Gi (Department of Food Science and Biotechnology, Kyungpook National University)
Nam, Ju-Ock (Department of Food Science and Biotechnology, Kyungpook National University)
Publication Information
Journal of Applied Biological Chemistry / v.63, no.2, 2020 , pp. 147-152 More about this Journal
Abstract
Farrerol is a flavanone isolated from the traditional Chinese herb 'Man-shan-hong' (Rhododendron dauricum L.). Farrerol has been reported to have various bioactivities including anti-oxidant, anti-inflammation, and anti-fungal. However, anti-cancer effect of farrerol has not yet been reported in MCF-7 breast cancer cells. In the present study, we investigated the anti-cancer effect of farrerol on MCF-7 cells. Farrerol decreased viability and induced apoptosis of MCF-7 cells in a dose dependent manner. Ferrerol exhibited a significant anti-proliferation effect with a half-maximal inhibitory concentration (IC50) values of 145.04±1.4 μM in MTT assay, when MCF-7 cells were treated with ferrerol for 48 h. Also, ferrerol induced apoptotic bodies of MCF-7 cells as evaluated by TUNEL assay and Annexin V/PI staining using FACS. By mechanism of action, ferrerol regulated the activation of p38 mitogen-activated protein kinase and altered the expression level of BAX, Bcl-2, and Poly ADP Ribose Polymerase in MCF-7 cells. In summary, our finding demonstrated that ferrerol has anti-cancer effect through regulating the activation and expression of apoptosis-related proteins in MCF-7 cells.
Keywords
Anti-cacner; Apoptosis; Breast cancer; Farrerol; MCF-7;
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