Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Anti-cancer effect of farrerol induced apoptosis through activating p38 MAPK in Human breast cancer MCF-7 cells

인간 유방암 세포주 MCF-7에 대한 farrerol의 p38 MAPK 활성화와 세포사멸 유도를 통한 항암 효과

  • Chae, Jongbeom (Department of Food Science and Biotechnology, Kyungpook National University) ;
  • Lee, Seul Gi (Department of Food Science and Biotechnology, Kyungpook National University) ;
  • Nam, Ju-Ock (Department of Food Science and Biotechnology, Kyungpook National University)
  • Received : 2020.02.27
  • Accepted : 2020.05.18
  • Published : 2020.06.30
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Abstract

Farrerol is a flavanone isolated from the traditional Chinese herb 'Man-shan-hong' (Rhododendron dauricum L.). Farrerol has been reported to have various bioactivities including anti-oxidant, anti-inflammation, and anti-fungal. However, anti-cancer effect of farrerol has not yet been reported in MCF-7 breast cancer cells. In the present study, we investigated the anti-cancer effect of farrerol on MCF-7 cells. Farrerol decreased viability and induced apoptosis of MCF-7 cells in a dose dependent manner. Ferrerol exhibited a significant anti-proliferation effect with a half-maximal inhibitory concentration (IC50) values of 145.04±1.4 μM in MTT assay, when MCF-7 cells were treated with ferrerol for 48 h. Also, ferrerol induced apoptotic bodies of MCF-7 cells as evaluated by TUNEL assay and Annexin V/PI staining using FACS. By mechanism of action, ferrerol regulated the activation of p38 mitogen-activated protein kinase and altered the expression level of BAX, Bcl-2, and Poly ADP Ribose Polymerase in MCF-7 cells. In summary, our finding demonstrated that ferrerol has anti-cancer effect through regulating the activation and expression of apoptosis-related proteins in MCF-7 cells.

Farrerol은 중국에서 거담제로 사용되어온 전통 한약제로 사용된 산진달래(만산홍, Rhododendron dauricum L.)에서 유래된 플라바논이다. Farrerol은 항산화, 항염증 및 항균 작용을 포함한 다양한 생리 활성이 보고되었다. 하지만 farrerol의 MCF-7에 대한 항암 작용은 아직 보고된 바가 없다. 본 연구에서 인간 유방암 MCF-7 세포에 대한 farrerol의 처리가 세포증식을 억제하고 apoptosis를 유도함을 입증하였다. MCF-7 세포에 ferrerol을 48시간 동안 처리했을 때, 이는 통계적으로 유의한 세포증식 효과를 나타냈으며 이의 IC50 값은 145.04±1.4 μM임을 확인하였다. 또한, farrerol이 세포사멸을 유도함을 TUNEL assay와 FACS를 이용한 Annexin V/PI 염색을 통해 검증하였다. 이러한 항암 효능의 작용기전으로써, farrerol의 처리가 BAX/Bcl-2 및 Caspase-3활성화와 PARP 분절화를 증가시켜 세포자살을 촉진한다는 것을 확인하였다. 결론적으로, 본 연구의 결과는 farrerol이 apopotosis 관련 단백질의 활성 및 발현조절을 통해 MCF-7 유방암세포에 대한 항암 효능을 갖는다는 것을 보여주고 있다.

Keywords

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