• 한국환경농학회지
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• 제23권4호
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• pp.234-239
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• 2004

[ $NO_3$ ]-N and T-N removal rates of cattail wetland cells were compared with those of reed wetland cells. The examined cells were a part of a pond-wetland system composed of two ponds in series and six wetland cells in parallel. Each wetland cell was 25m in length and 6m in width. Cattails (Typha angustifolia) were transplanted into three cells and reeds Phragmites australis) into another three ones in June 2000. Water of Sinyang stream flowing into Kohung Estuarine lake located in the southern part of the Korean Peninsula was pumped into the primary pond, its effluent was discharged into the secondary pond Effluent from the secondary pond was funneled into each cell. Two cattail and reed cells were chosen for this research. Water quantity and quality of influnt and effluent were analyzed front May 2001 through October 2001. The volume of influent and effluent of the cells averaged about $20.0\;m^3/day$ and $19.3\;m^3/day$, respectively. Hydraulic retention time was approximately 1.5 days. Influent $NO_3$-N concentration for the four cells averaged 2.39 mg/L. Effluent $NO_3$-N concentration far the cattail and reed cells averaged 1.74 and 1.78 mg/L, respectively. Average $NO_3$-N retention rate for the cattail and reed cells by mass was 30 and 29%, respectively. Influent T-N concentration far the four cells averaged 4.13 mg/L. Effluent T-N concentration for the cattail and reed cells averaged 2.55 and 2.61 mgL respectively. Average T-N retention rate for the cattail and reed cells by mass was 39 and 38%, respectively. $NO_3$-N and T-N concentrations in effluent from the cattail cells were significantly low (p=0.04), compared with those from the reed cells. Cattail wetland cells were more efficient for $NO_3$-N and T-N abatement than reed ones.

• 동의생리병리학회지
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• 제20권5호
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• pp.1315-1320
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• 2006

The root and rhizomes of Nardostachys chinensis belonging to the family Valerianaceae has been used for medicinal therapy in Korean traditional medicine. The parts have been especially used to elicit stomachic and sedative effects. Our previous studies reported that the water extract of N. chinensis has induced granulocytic differentiation inhuman promyelocytic leukemia (HL-60) cells. The Mitogen-activated protein kinases (MAPKs) are serine/threonine kinases involved in the regulation of various cellular responses, such as cell proliferation, differentiation and apoptosis. In this study, we investigated the signaling pathways on the HL-60 cell differentiation induced by N. chinensis. Activation of extracellular signal-regulated kinase (ERK) increased time-dependently in differentiation of HL-60 cells induced by N. chinersis. Activation of p38 increased slightly at 24 h after N. chinensis treatment, but activation of c-jun N-terminal kinase (JNK) was unaffected. Inhibitor of ERK (PD98059) significantly reduced NBT reduction activity induced by N. chinensis in HL-60 cells. In contrast, p38 inhibitor (SB203580) did not inhibit the cell differentiation. These results indicated that activaiton of ERK may De involved in HL-60 cell differentiation induced by N. chinensis.

• 한국초지조사료학회지
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• 제4권2호
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• pp.122-126
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• 1983

本 硏究는 土壞含水量이 바른 2個의 억새草地를 對象으로 하여 억새가 生産한 物質을 어떻게 植物의 各 器管에 分配하는가를 硏究하였다. 그 結果를 要約하면 다음과 같다. 1. 土壤含水量이 22.54%인 억새 草地 A와 9.22%인 억새草地 B間에 現存量의 極大値는 各名 2,267. 12g/m$^{2}$와 943.44g/m$^{2}$로 大端히 有意한 差가 있었으며 物質生産力의 peak는 9月로 鍾型曲線을 나타내었다. 2 억새가 合成한 養分은 地下莖에 저장하여 次年度의 生長을 준비하며 地上部에서는 열매를 맺어 種子를 生産하였다. 3 物質生産構造를 比較한 결과 全體的인 型에 있어서는 禾木科植物의 特徵을 나타내나 土壤含水量이 적은 억새草地B에서 이삭의 乾物生産量이 他器管의 것에 比하여 많았다. 4. 地上部의 生殖器管에 生産物質을 分配하는 比率은 억새草地A에서 0.6%, 억새草地B에서 1.4%였다. 5. 地下莖의 生産物質의 分配率은 억새草地A에서 35.4%, 억새草地B에서 46.6%였다. 6 地上部와 地下部의 生殖器管의 生産物質 分配準은 억새초지A에서 36.0%, 억새초지B에서 48.0%였다. 이 結果로 억새는 乾燥하면 生産物質을 生殖器管에 보다 많은 量을 分配한다는 것을 알 수 있다.

• Journal of Nutrition and Health
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• 제38권8호
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• pp.656-662
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• 2005

Ginger (Zingiber of oficinale Roscoe, Zingiberaceae) is one of the most frequently and heavily consumed dietary condiments throughout the world. Besides its extensive use as a spice, the rhizome of ginger has also been used in traditional oriental herbal medicine for the management of symptoms such as common cold, digestive disorders, rheumatism, neurologia, colic, and motion-sickness. The oleoresin from rhizomes of ginger contains [6] -gingerol (1- [4'-hydroxy-3'-methoxyphenyl]-5-hydroxy-3-decanone) and its homologs as pungent ingredients that have been found to possess many interesting pharmacological and physiological activities, such as anti-inflammatory, analgesic, antipyretic, antiheatotoxic, and cardiotonic effects. However, the effect of [6]-gingerol on cell proliferation in breast cancer cell are not currently well known. Therefore, in this study, we examined effect of [6]-gingerol on protein and mRNA expression associated with cell proliferation in MDA-MB-231 human breast. cancer cell lines. We cultured MDA-MB-231 cells in presence of 0, 2.5, 5 and $10{\mu}M$ of [6] -gingerol. [6]-Gingerol inhibited breast cancer cell growth in a dose-depenent manner as determined by MTT assay. ErbB2 and ErbB3 protein and mRNA expression were decreased dose-dependently in cells treated with [6]-gingerol (p<0.05). In addition, phosphorylated Akt levels and total hぉ levels were markedly decreased in cells treated with $2.5{\mu}M$ [6]-gingerol (p<0.05). In conclusion, we have shown that [6]-gingerol inhibits cell proliferation through ErbB2 and ErbB3, reduction in MDA-MB-231 human breast cancer cell lines.

• Biomolecules & Therapeutics
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• 제29권6호
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• pp.658-666
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• 2021

Podophyllotoxin (PT), a lignan compound from the roots and rhizomes of Podophyllum peltatum, has diverse pharmacological activities including anticancer effect in several types of cancer. The molecular mechanism of the anticancer effects of PT on colorectal cancer cells has not been reported yet. In this study, we sought to evaluate the anticancer effect of PT on human colorectal cancer HCT116 cells and identify the detailed molecular mechanism. PT inhibited the growth of cells and colony formation in a concentration-dependent manner and induced apoptosis as determined by the annexin V/7-aminoactinomycin D double staining assay. PT-induced apoptosis was accompanied by cell cycle arrest in the G2/M phase and an increase in the generation of reactive oxygen species (ROS). The effects of PT on the induction of ROS and apoptosis were prevented by pretreatment with N-acetyl-L-cysteine (NAC), indicating that an increase in ROS generation mediates the apoptosis of HCT116 cells induced by PT. Furthermore, Western blot analysis showed that PT upregulated the level of phospho (p)-p38 mitogen-activated protein kinase (MAPK). The treatment of SB203580, a p38 inhibitor, strongly prevented the apoptosis induced by PT, suggesting that PT-induced apoptosis involved the p38 MAPK signaling pathway. In addition, PT induced the loss of mitochondrial membrane potential and multi-caspase activation. The results suggested that PT induced cell cycle arrest in the G2/M phase and apoptosis through the p38 MAPK signaling pathway by upregulating ROS in HCT116 cells.

• Natural Product Sciences
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• 제25권3호
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• pp.238-243
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• 2019

In this study, the marker compounds of Curcumae Rhizoma (CR) were simultaneously quantified by high-performance liquid chromatography equipped with a photodiode array detector and the anti-inflammatory effects of CR extract and marker compounds in human benign prostatic hyperplasia epithelial-1 (BPH-1) cell lines were investigated. The marker components (4S,5S)-(+)-germacrone-4,5-epoxide, furanodienone, and germacrone, were separated on Gemini $C_{18}$ columns ($250mm{\times}4.6mm$, $5{\mu}m$) at $40^{\circ}C$ by using a gradient of two mobile phases eluting at 1.0 mL/min. Prostaglandin $E_2$ ($PGE_2$) levels in Human BPH-1 cells were determined with an ELISA kit. The coefficients of determination in a calibration curve of each analyte were all 0.9997. The limits of detection and quantification of the three compounds were $0.10-0.32{\mu}g/mL$ and $0.30-0.98{\mu}g/mL$, respectively. The content of three compounds, (4S,5S)-(+)-germacrone-4,5-epoxide, furanodienone, and germacrone, in the CR sample were found to be 5.79 - 5.92 mg/g, 4.72 - 4.86 mg/g, and 1.06 - 1.09 mg/g, respectively. Regarding pharmacological activity against benign prostatic hyperplasia, CR and its components significantly suppressed $PGE_2$ levels of BPH-1 cells. The established analysis method will help to improve quality assessment of CR samples and related products. In addition, CR and its components exhibit antiinflammatory activity in BPH-1 cells, suggesting the inhibitory efficacy of these compounds against the pathogenesis of BPH.

• 한국전자통신학회논문지
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• 제16권3호
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• pp.493-502
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• 2021

외부 환경변화에 적응하여 MPP를 추적할 수 있는 포복경 영양 번식(VPR; Vegetative Propagation by Runner) 최적화 알고리즘 기반 MPPT 알고리즘을 제시하였다. VPR 알고리즘은 영양기관을 기반으로 군집 이동 번식하는 식물 생태를 모방한 알고리즘으로 식물의 노화 및 부근(Rhizome)에 대한 주변탐색 절차를 수행하여 최적점 인근의 주변을 지속적으로 탐색할 수 있다. 따라서 VPR 기반 MPPT 알고리즘의 경우, MPPT 알고리즘이 수행되는 시점에 발생하는 외부 환경변화에 적응하여 최적점을 탐색할 수 있다. 본 논문에서는 다수의 모의실험을 통해 VPR 기반 MPPT 알고리즘의 성능을 분석하였다. 더불어 PSO(Particle Swarm Optimization) 기반 MPPT 알고리즘과 동일한 환경에서 성능 비교를 통해 성능의 우수성을 비교하였다.

• 대한본초학회지
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• 제36권3호
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• pp.25-37
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• 2021

Objectives : Herbal medicines have been used a lot traditionally in Northeast Asia. In particular, various Apiaceae plants (Umbelliferae) are widely used for medicinal purposes among countries. However, the original species designated in each pharmacopoeia standards, are sometimes different and confuse. In this study, herbal medicines in the pharmacopoeia were analyzed for Apiaceae taxa to compare the taxonomic identity and different taxonomic opinions for each country based on their local flora and recent taxonomic studies. Methods : The scientific names of herbal medicines were analyzed origins from Pharmacopoeias of Republic of Korea, Democratic People's Republic of Korea, People's Republic of China, Taiwan, and Japan. Especially, we compared their local floras, international plant scientific name database, and recently published taxonomic studies to confirm the correct scientific name. Results : The analyzed apiaceous herbal medicines in pharmacopoeias were all 21 items and 39 taxa; the highest percentage, genus Angelica (21%) and roots including rhizomes (62%) the registered genera and medicinal parts, respectively. Eleven items were in all five country's pharmacopoeias. Four items were in more than two countries' pharmacopoeias. Six items were registered in only one pharmacopoeia. Conclusions : Our research provides necessary information, e.g., corrected scientific names of original species based on taxonomic species concepts, taxonomic reviews, and figures of medicines. These results will help in the understanding of the apiaceous taxa among the five countries' pharmacopoeias. Moreover, these extensive nomenclatural reviews of authentic apiaceous taxa will help for the next revision of Korea Pharmacopoeia.

• Ecology and Resilient Infrastructure
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• 제9권4호
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• pp.247-258
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• 2022

함평만은 좁은 만 입구와 복잡한 지형 구조로 인하여 비대칭적인 퇴적물 이동이 일어나기 때문에, 갯벌의 저질에는 모래 함량이 증가하는 것으로 연구되었다. 모래화 갯벌은 염습지 식생 분포를 변화시키는 것으로 현존식생도와 식생단면도 조사를 통해 분석되었다. 2016년과 2022년의 식생면적 비교에서 절대염생식물인 해홍나물군락은 74% 감소하였다. 반면 임의염생식물인 갯잔디군락은 75% 증가하였다. 특히 갯잔디는 지하경과 줄기의 밀도가 높게 발달하여, 군락 내 모래와 같은 퇴적물을 가둬서 견고한 사구환경을 조성하는 것으로 보인다. 함평만 갯벌의 효과적인 자연보전 관리 계획 수립을 위하여, 갯벌 토성 변화와 식생 군집의 상호작용이 향후 갯벌생태계에 미치는 영향에 대해 통합적 접근이 필요하다.

• 대한본초학회지
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• 제27권6호
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• pp.63-69
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• 2012

Objectives : Decoction, in Korean Medicine, is a pharmacological method of extraction, by boiling, of dissolved chemicals, or herbal prescriptions, which may include stems, roots, bark and rhizomes. Decoctions differ from most teas, infusions, in that they are usually boiled. This study was performed to compare the difference of water decoctions extracted by different decoction extractor and extraction time and to analyze the reason of decoctions extracted by each decoction extractor have different taste. Methods : With water decoction samples by Pressure extractor, Non-pressure extractor and Ultrasonic waves merge extractor for 1 hr, 2 hr and 3 hr were investigated the yield and the concentration of hesperidin and glycyrrhizin by HPLC/DAD system in Pyungwi-san decoction. Results : The samples of each extractor were gradually increased the yield and the concentration of hesperidin and glycyrrhizin. The HPLC pattern of samples is similar. The yield and the concentration of hesperidin and glycyrrhizin of Ultrasonic waves merge extractor was most highest of the three. The rate of increase of the yield and the hesperidin concentration of between 1 hr and 2 hr in Pressure extractor was the most highest of the three. But the concentration of glycyrrhizin in Pressure extractor was relatively similar to Non-pressure extractor. Conclusions : The yield and the concentration of reference compounds in Pyungwi-san water decoction was influenced by extracting method and extracting time. For scientific movement and standardization of extracting medicinal herbs method need to study of extractor validation and to study in vitro and in vivo.