Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Extracellular Signal-regulated Kinase (ERK) is Required for Water Extract of Nardostachys chinersis-Induced Differentiation in HL-60 Cells

  • Yoon, Sang-Hak (Department of Oriental Regabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Ju, Sung-Min (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Nam-Su (Department of Oriental Regabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Park, Sung-Cheol (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Park, Jin-Young (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Sung-Hoon (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University) ;
  • Song, Yung-Sun (Department of Oriental Regabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University)
  • Published : 2006.10.25
Download PDF
⟨ Previous Next ⟩

Abstract

The root and rhizomes of Nardostachys chinensis belonging to the family Valerianaceae has been used for medicinal therapy in Korean traditional medicine. The parts have been especially used to elicit stomachic and sedative effects. Our previous studies reported that the water extract of N. chinensis has induced granulocytic differentiation inhuman promyelocytic leukemia (HL-60) cells. The Mitogen-activated protein kinases (MAPKs) are serine/threonine kinases involved in the regulation of various cellular responses, such as cell proliferation, differentiation and apoptosis. In this study, we investigated the signaling pathways on the HL-60 cell differentiation induced by N. chinensis. Activation of extracellular signal-regulated kinase (ERK) increased time-dependently in differentiation of HL-60 cells induced by N. chinersis. Activation of p38 increased slightly at 24 h after N. chinensis treatment, but activation of c-jun N-terminal kinase (JNK) was unaffected. Inhibitor of ERK (PD98059) significantly reduced NBT reduction activity induced by N. chinensis in HL-60 cells. In contrast, p38 inhibitor (SB203580) did not inhibit the cell differentiation. These results indicated that activaiton of ERK may De involved in HL-60 cell differentiation induced by N. chinensis.

Keywords

References

  1. Pharmacopoeia of the People's Republic of China, Vol. I; Chemical Industry Press: Beijing p 65, 2000
  2. Xiao, P.G. Modern Chinese Materia Medica; Chemical Industry Press: Beijing p 252, 2002
  3. Takaya, Y., Takeuji, Y., Akasaka, M., Nakagawasai, O., Tadano, T., Kisara, K., Kim, H.S., Wataya, Y., Niwa, M. and Oshima, Y. Novel Guaiane Endoperoxides, Nardoguaianone A-D, from Nardostachys chinensis Roots and their Antinociceptive and Antimalarial Activities. Tetrahedron 56:7683-7678, 2000
  4. Itokawa, H., Masuyama, K., Morita, H. and Takeya, K. Cytotoxic sesquiterpenes from Nardostachys chinensis. Chem. Pharm. Bull. (Tokyo) 41:1183-1184, 1993 https://doi.org/10.1248/cpb.41.1183
  5. Li, P., Matsunaga, K., Yamakuni, T. and Ohizumi, Y. Nardosinone, the first enhancer of neurite outgrowth- promoting activity of staurosporine and dibutyryl cyclic AMP in PC12D cells. Brain Res. Dev. Brain Res. 145:177-183, 2003 https://doi.org/10.1016/S0165-3806(03)00239-6
  6. Ju, S.M., Lee, J., Choi, H.S., Yoon, S.H., Kim, S.H. and Jeon, B.H. Effect of Nardostachyos Rhizoma on apoptosis, Differentiation and Proliferation in HL-60 cells. Korean. J. Orient. Physiol. Phathol. 20:163-170, 2006
  7. Huberman, E, and Callaham, M.F. Induction of terminal differentiation in human promyelocytic leukemia cells by tumor-promoting agents. Proc. Natl. Acad. Sci. USA 76:1293-1297, 1979
  8. Rovera, G., Santoli, D. and Damsky, C. Human promyelocytic leukemia cells in culture differentiate into macrophage-like cells when treated with a phorbol diester. Proc. Natl. Acad. Sci. USA 76:2779-2783, 1979
  9. Collins, S.J., Ruscetti, F.W., Gallagher, R.E. and Gallo, R.C. Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds. Proc. Natl. Acad. Sci. USA 75:2458-2462, 1978
  10. Pan, Q., Granger, J., O'Connell, T.D., Somerman, M.J. and Simpson, R.U. Promotion of HL-60 cell differentiation by 1,25-dihydroxyvitamin D3 regulation of protein kinase C levels and activity. Biochem. Pharmacol. 54:909-915, 1997 https://doi.org/10.1016/S0006-2952(97)00286-4
  11. Kharbanda, S., Saleem, A., Emoto, Y., Stone, R., Rapp, U. and Kufe, D. Activation of Raf-1 and mitogen-activated protein kinases during monocytic differentiation of human myeloid leukemia cells. J. Biol. Chem. 269:872-878, 1994
  12. Kim, S.H., Oh, S.M. and Kim, T.S. Induction of human leukemia HL-60 cell differentiation via a PKC/ERK pathway by helenalin, a pseudoguainolide sesquiterpene lactone. Eur. J. Pharmacol. 511:89-97, 2005 https://doi.org/10.1016/j.ejphar.2005.02.008
  13. Kim, S.H., Kim, H.J. and Kim, T.S. Differential involvement of protein kinase C in human promyelocytic leukemia cell differentiation enhanced by artemisinin. Eur. J. Pharmacol. 482:67-76, 2003 https://doi.org/10.1016/j.ejphar.2003.09.057
  14. Cross, T.G., Scheel-Toellner, D., Henriquez, N.V., Deacon, E., Salmon, M. and Lord, J.M. Serine/threonine protein kinases and apoptosis. Exp. Cell Res. 256:34-41, 2000 https://doi.org/10.1006/excr.2000.4836
  15. Pearson, G., Robinson, F., Beers Gibson, T., Xu, B.E., Karandikar, M., Berman, K. and Cobb, M.H. Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions. Endocr. Rev. 22:153-183, 2001 https://doi.org/10.1210/er.22.2.153
  16. Cobb, M.H. MAP kinase pathways. Prog. Biophys. Mol. Biol. 71:479-500, 1999 https://doi.org/10.1016/S0079-6107(98)00056-X
  17. Xia, Z., Dickens, M., Raingeaud, J., Davis, R.J. and Greenberg, M.E. Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis. Science 270:1326-1331, 1995 https://doi.org/10.1126/science.270.5240.1326
  18. Davis, R.J. Signal transduction by the JNK group of MAP kinases. Cell 103:239-252, 2000 https://doi.org/10.1016/S0092-8674(00)00116-1
  19. Yen, A., Roberson, M.S., Varvayanis, S. and Lee, A.T. Retinoic acid induced mitogen-activated protein (MAP)/extracellular signal-regulated kinase (ERK) kinase-dependent MAP kinase activation needed to elicit HL-60 cell differentiation and growth arrest. Cancer Res. 58:3163-3172, 1998
  20. Yen, A., Roberson, M.S. and Varvayanis, S. Retinoic acid selectively activates the ERK2 but not JNK/SAPK or p38 MAP kinases when inducing myeloid differentiation. In Vitro Cell Dev. Biol. Anim. 35:527-532, 1999 https://doi.org/10.1007/s11626-999-0063-z
  21. Wang, X. and Studzinski, G.P. Activation of extracellular signal-regulated kinases (ERKs) defines the first phase of 1,25-dihydroxyvitamin D3-induced differentiation of HL60 cells. J. Cell Biochem. 80:471-482, 2001 https://doi.org/10.1002/1097-4644(20010315)80:4<471::AID-JCB1001>3.0.CO;2-J
  22. Wang, X., Rao, J. and Studzinski, G.P. Inhibition of p38 MAP kinase activity up-regulates multiple MAP kinase pathways and potentiates 1,25-dihydroxyvitamin D(3)-induced differentiation of human leukemia HL60 cells. Exp. Cell Res. 258:425-437, 2000 https://doi.org/10.1006/excr.2000.4939
  23. Wang, X. and Studzinski, G.P. Inhibition of p38MAP kinase potentiates the JNK/SAPK pathway and AP-1 activity in monocytic but not in macrophage or granulocytic differentiation of HL60 cells. J. Cell Biochem. 82:68-77, 2001 https://doi.org/10.1002/jcb.1141
  24. Sakashita, A., Nakamaki, T., Tsuruoka, N., Honma, Y. and Hozumi, M. Granulocyte colony-stimulating factor, not granulocyte-macrophage colony-stimulating factor, co-operates with retinoic acid on the induction of functional N-formyl-methionyl-phenylalanine receptors in HL-60 cells. Leukemia 5:26-31, 1991
  25. Breitman, T.R., Selonick, S.E. and Collins, S.J. Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid. Proc. Natl. Acad. Sci. USA 77:2936-2940, 1980
  26. Collins, S.J., Ruscetti, F.W., Gallagher, R.E. and Gallo, R.C. Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds. Proc. Natl. Acad. Sci. USA 75:2458-2462, 1978
  27. Rovera, G., Santoli, D. and Damsky, C. Human promyelocytic leukemia cells in culture differentiate into macrophage-like cells when treated with a phorbol diester. Proc. Natl. Acad. Sci. USA 76:2779-2783, 1979
  28. Tanaka, H., Abe, E., Miyaura, C., Shiina, Y. and Suda, T. 1alpha,25-dihydroxyvitamin D3 induces differentiation of human promyelocytic leukemia cells (HL-60) into monocyte-macrophages, but not into granulocytes. Biochem. Biophys. Res. Commun. 117:86-92, 1983 https://doi.org/10.1016/0006-291X(83)91544-9
  29. Gate, L., Lunk, A. and Tew, K.D. Resistance to phorbol 12-myristate 13-acetate-induced cell growth arrest in an HL60 cell line chronically exposed to a glutathione S-transferase pi inhibitor. Biochem. Pharmacol. 65:1611-1622, 2003 https://doi.org/10.1016/S0006-2952(03)00152-7