• Korean Journal of Medicinal Crop Science
• /
• v.8 no.2
• /
• pp.109-116
• /
• 2000

This study was carried out to screen herbs among herbal plants showing antimicrobial activity against some food spoilage and pathogenic microorganisms. One hundred fifteen kinds of herbal plants were extracted by 70% ethanol, and then they have been screened for antimicroorganisms. Six herbal plants such as Salviae radix, Dryopteris rhizoma, Terminaliae fructus, Araliae radix, Psoraleae fructus and Schisandrae fructus showed strong antimicrobial activities against Bacillus subtilis. Antimicrobial activities were showed in Anemarrhena radix and Dryopteris rhizoma on Candida albicans, and in Anemarrhenae radix, Dryopteris rhizoma and Polygalae radix on Schizosaccharomyces sp. It was revealed that eight herbal plants such as Dryopteris rhizoma, Salviae radix, Sappan ligunum, Sinomeniae radix, Schisandrae fructus, Rhui fructus, Sophorae radix and Inulae radix also showed antimicrobial activities on Streptococcus mutans. In addition, Anemarrhena radix, Curcuma tuber, Inulae radix, Polygonum radix, Sanguisorbae radix, Scutellariae radix and Terminaliae fructus and showed antimicrobial activities on Trichophyton mentagrophytes. Four kinds of herbal plants such as Dropteris rhizoma, Salviae radix, Terminaliae fructus and Scutellariae radix which showed broad antimicrobial spectrums were mixed by 1 : 1 ratio with the other herbal paints showing relatively strong microbial activities such as Terminaliae fructus, Sinomeniae radix and Scutellariae radix etc. The extracts of mixed herbal paints showed higher antimicrobial activities than those of single herbal plant.

• Biomolecules & Therapeutics
• /
• v.12 no.3
• /
• pp.151-156
• /
• 2004

This study was carried out to evaluate the putative anxiolytic-1ike effects of the aqueous extracts of Sanjoin-tang (SJIT) and its ingredients using the elevated plus maze (EPM) test in mice. SJIT consists of five herbs, namely, Zizyphi Spinosi Semen (roasted), Glycyrrhizae Radix, Cnidii Rhizoma, Anemarrhenae Rhizoma, and Hoelen. The aqueous extracts of SJIT and each herbal drug were orally administered to ICR mice, 1 hr before evaluating behavioral activity in the EPM test, respectively. Repeated treatments (for 3 days) of the aqueous extract of SJIT (400 mg/kg) significantly increased time-spend in the open arms and arms entries into the open arms in the EPM test. Zizyphi Spinosi Semen (400 mg/kg), an ingredient of SJIT, significantly increased timespent in the open arms and arm entries into the open arms (P < 0.05). However, the other ingredient of SJIT did not show any anxiolytic-like behaviors. In addition, the anxiolytic-like effects of Zizyphi Spinosi Semen were blocked by pindolol (lO mg/kg), a $5-HT_{1A}$ receptor antagonist. These results suggest that Zizyphi Spinosi Semen (roasted) as an ingredient of SJIT plays a crucial anxiolytic role, and it acts via the serotonergic nervous system.

• The Journal of Pediatrics of Korean Medicine
• /
• v.8 no.1
• /
• pp.13-26
• /
• 1994

Experimental studies were done to research the clinical effects of Sipyungtang and Sipyungtang-gamibang (sipyungtang with Anemarrhenae Rhizoma added) on the Anti-allergic effect. The results obtained as follows; 1. In the effects of Sipyungtang and Sipyungtang-gamibang on vascular permeability responses to intradermal Histamine, Sipyungtang group revealed none significant effect, but Sipyungtang-gamibang group revealed significant effect. 2. In the effects of sipyungtang and Sipyungtang-gamibang on vascular permeability responses to intradermal serotonin, Sipyungtang group revealed none significant effect, but Sipyungtang-gamibang group revealed significant effect. 3. In the 48hrs homologous passive cutaneous anaphylaxis provoked by the IgE-like antibody against egg white albumin, Sipyungtang and Sipyungtang-gamibang groups revealed none significant effect. 4. In the delayed type hypersensitivity responses to Picryl Chloride, Sipyungtang and Sipyungtang-gamibang groups revealed none significant effect. 5. In the delayed type hypersensitivity responses to SRBC, Sipyung-tang and Sipyungtang-gamibang groups revealed significant effect.

• The Journal of Internal Korean Medicine
• /
• v.13 no.1
• /
• pp.1-20
• /
• 1992

The study was carried out to investigate the effect of Kwandongwhasan extract and its constituent herbs onthe contractile force of isolated guineapig trachealis muscle and to elucidate its mechanism. The results of this study were as follows; 1. Kwandongwhasan significantly inhibited the contractile response of isolated guinea pig trachealis muscle by histamine. 2. Kwandongwhasan significantly inhibited the contractile response of isolated guinea pig trachealis muscle by acetylcholine. 3. Kwandongwhasan significantly inhibited the contractile response of isolated guinea pig trachealis muscle by 5-hydrooxytryptamine. 4. Kwandongwhasan significantly inhibited the contractile response of isolated guinea pig trachealis muscle by prostaglandin $F2\;{\alpha}$ 5. Herba Ephedrae (麻黃), Semen Armeniacae(杏仁), Cortex Mormi(紫白皮), and Flos Farfarae (款冬花), extract significantly inhibited the contractile response of isolated guinea pig trachealis muscle by histamine, acetylcholine, 5-hydroxytryptamine and prostaglandin $F2\;{\alpha}$. 6. Radix Glycyrrhdzae(甘草) extract significantly inhibited the contractile response of isolated guinea pig trachealis muscle by prostaglandia $F2\;{\alpha}$. 7. Rhizoma Anemarrhenae(知母) extract significantly inhibited the contractile response of isolated guinea pig trachealis muscle by histamine. 8. Bulbus Fritillariae(貝母) and Tuber Pinelliae(半夏) extract did not inhibit the significantly inhibited the contractile response of isolated guinea pig trachealis muscle.

• Natural Product Sciences
• /
• v.15 no.4
• /
• pp.203-207
• /
• 2009

A homoisoflavanone, 7,4'-dihydroxyhomoisoflavanone (1) and a flavanone, (2S)-7,4'-dihydroxy-5-methoxyflavanone (2), were isolated from the rhizomes of Anemarrhena asphodeloides, together with 4,4'-dihydroxychalcon (3), 2'-O-methylphlorethin (4), 1,3-bis-di-p-hydroxyphenyl-4-penten-1-one (5), and 2,4'-dihydroxy-4-methoxybenzophenone (6) on the basis of spectroscopic and physicochemical analyses including 1Dand 2D- NMR techniques as well as by comparison of their data with the published values. Compounds 1 - 4 were isolated for the first time from this plant source. Among isolates, compound 2 exhibited moderate inhibitory effect on the differentiation of pre-adipocyte 3T3-L1 cells.

• Journal of Environmental Science International
• /
• v.32 no.1
• /
• pp.67-76
• /
• 2023

Under constant environmental pollution, the incidence of Atopic Dermatitis (AD) caused by air pollutants and allergens has increased. AD is an allergy inflammatory skin disease characterized by pruritus, eczema, and skin dryness. In herbal medicine, Anemarrhena asphodeloides (Anemarrhenae Rhizoma; AR) has been utilized to treat Alzheimer's disease, osteoporosis, hypertension, and inflammation. The purpose of study evaluated the effect of AR in a mouse model of 2,4-dinitrochlorobenzene (DNCB)-induced AD-like skin lesions. After acclimatization for 5 days, the mice (6-week-old, male Balb/c) were divided into five groups (n=6/group): NC (normal control), DNCB (control), Dex (5 mg·kg-1, p.o.), AR100 (100 mg·kg-1, p.o.), and AR300 (300 mg·kg-1, p.o.). On days 1 and 3, 1% DNCB was applied to the skin and ears. After 4 days, 0.5% DNCB was applied once every 2 days for 2 weeks. Then, skin and ears eczema area and severity index (EASI); skin nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2) levels; and plasma immunoglobulin E (IgE) levels were examined. The AR groups showed lower EASI, skin and ear thickness, mast cell count, and IgE levels than the control groups. Moreover, AR reduced iNOS, COX-2, and PGE2 levels. Therefore, AR possesses anti-inflammatory properties and can improve skin damage, indicating its therapeutic potential against AD.

• Herbal Formula Science
• /
• v.31 no.4
• /
• pp.373-388
• /
• 2023

Objectives : This study aimed to provide basic data for research by investigating non-clinical experimental studies on herbal medicines and its compounds for precocious puberty. Methods : A search was conducted for all literature until October 2023 using combinations of keywords such as precocious puberty, puberty, and chinese medicine in three databases (Pubmed, OASIS, and ScienceON). Results : 1. In animal experiments, studies were mainly conducted using a model that induced precocious puberty by subcutaneously administering danazol to SD rats on the 5th day after birth, and in cell experiments, precocious puberty was induced by treating GT1-7 cells with kisspeptin 10 or estradiol. 2. Anemarrhenae Rhizoma, Phellodendri Cortex, and Prunellae Spica were mainly used as herbal medicine to evaluate their efficacy on precocious puberty in non-clinical experiments. 3. Macroscopic observation, hematological analysis, histological analysis, and genetic analysis were performed as methods to analyze the experimental results. Conclusions : In this study, the effects of herbal medicine on precocious puberty and non-clinical research methods were confirmed. Based on the results of this study, it is expected that non-clinical effectiveness and mechanism research on materials that are clinically effective in Traditional Korean Medicine will be revitalized.

• The Journal of Dong Guk Oriental Medicine
• /
• v.9
• /
• pp.139-154
• /
• 2000

This studies were examined in orther to investigate the object and the method of animal experimental papers on medicinal herbs of cancer therapeutic activities from the reported 23 literatures containing anti-cancer effects of medicinal herbs. The results were obtained as follows: 1. The oriental medicinal therapies on cancer were Pujeung(扶正法), Kuesa(祛邪法), Pujeungkuesa(扶正法邪法). 2. The experimental medicinal herbs of cancers therapy were 103 species, which was used for experimental cancer single or combine. Among then, Houttuyniae herba, Polyporus, Manitis squama, Evodiae fructus, Aucklandiae radix and Pharbitidis semen were effective for cancer treatment, while Houttuyniae herba inhibited tumor cells, but not normal cells. Also, Evodiae fructus, Aucklandiae radix and Pharbitidis semen showed strong cytoxicities on 20 different tumor cell lines, whereas Saururi herba seu rhizoma showed cytoxicity against HT-29 cell, melanoma, SK-MEL-5 cell, and Anemarrhenae rhizoma against ovarian tumor cell only, and Schizonepetae herba against HT-29 cell line only with a potent inhivitory activities. 3. P815 cell, Yac-1 cell, Sarcoma 180 cell, K 562 cell, and SNU-1 cells were frequently used as experimental cancer therapy.

• Journal of Food Hygiene and Safety
• /
• v.29 no.2
• /
• pp.152-157
• /
• 2014

This study was carried out to investigate the residual sulfur dioxide residues ($SO_2$) in commercial medicinal herbs in Korea in 2013. Among a total of 116 samples of 11 different kinds of herbs, 71 samples (11 Kinds) were domestic, and 45 samples (10 Kinds) were imported. The residual contents of $SO_2$ in the samples were determinated by a modified Monier-Williams method. Of the 116 samples, 6 samples (5.2%, 4 Kinds) failed to meet the regulations for sulfur dioxide residues of KFDA in medicinal herbs. Among 6 unsuitable samples, 1 samples (1.4%) were domestic, and 5 samples (11.1%, 3 Kinds) were imported. The highest amount of sulfur dioxide residues was 1546.3 mg/kg (Lycii Fructus) in the domestic samples. Regardless of region, $SO_2$ contents were not found at Anemarrhenae Rhizoma, Citri Unshius Pericarpium, Eucommiae Cortex, Forsythiae Fructus, Gardeniae Fructus, Leonuri Herba and Lonicerae Folium et caulis. These data will be used as a basic data for the future legislation on the regulation and control of sulfur dioxide of herbal medicines.

• Korean Journal of Food Science and Technology
• /
• v.32 no.5
• /
• pp.1206-1212
• /
• 2000

This study was conducted to know whether Gamiyookmijihwangtang(GY) which is Yookmijihwang added with Liriopis tuber, Anemarrhenae rhizoma and Phellodendri cortex can remedy the overt diabetes in diabetes-prone BB(BBDP) rats. The rats were given GY through the mother from the fetal stage until birth. After birth they received GY through breast feeding until 20 days old. From 21 days old which is the beginning of the weaning period 60 BB rats(30 males and 30 females) were divided into 2 experimental groups(BBDP and BBDP-GY) and placed individually in metabolic cages. BBDP was the control group which didn't receive any GY and BBDP-GY received 16 mL/㎏ B.W./day of GY until 120 days old. The antidiabetic effects of GY were characterized by the clinical features such as polyurea, polydipsia, hyperglycaemia and the rapid loss of body weight. Body weight, water consumption, urine volume and blood glucose level showed no signs of impending diabetes but after onset there were big changes in those parameters. The onset of diabetes was delayed and the incidence of diabetes was also much decreased with GY but after onset there were no beneficial effects from it.