• FLOWER RESEARCH JOURNAL
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• v.19 no.2
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• pp.115-118
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• 2011

A light pink single freesia (Freesia hybrida Hort.) 'Bolero' was developed from a cross of breeding line ('Yellow Ballet' and 'Yellow Dream') and 'Sandra' followed by seedling and line selections at the National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA) in 2007. Growth and flowering characteristic tests were conducted from 2003 to 2006. 'Bolero' has single type flower with light pink color (RHS, R55B). It has multiple florets, stable flower shape, upright leaves and stalks. The average cut flower yield of 'Bolero' observed in the greenhouse from 2005 to 2007 was 7.5 cuttings per plant. It has good production of cormlets and vigorous growth. 'Bolero' could be cultured under greenhouse conditions in Korea.

• Polymer(Korea)
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• v.33 no.2
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• pp.118-123
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• 2009

Keratin contains regulatory molecules that can enhance neuronal cell activity. We fabricated keratin/ PLGA films using 0, 10, 20, and 50 wt% of keratin using solvent casting method. We measured the contact angle of each film and cell proliferation was assayed by counting the cells attached on the film. Adhered cell morphology was confirmed by scanning electron microscope. RT-PCR was conducted to evaluate the gene expression of NF, NSE, and S-100, the Schwann cell markers. The keratin content of 20 and 50 wt% provided higher wettability than PLGA. The 20 wt% keratin was better in cell adhesion and proliferation of SCs than other keratin/PLGA films. The phenotypic stability of SC was maintained with the keratin content of 10 and 20 wt%.

• Polymer(Korea)
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• v.32 no.5
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• pp.403-408
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• 2008

Keratin is the major structural fibrous protein providing outer covering such as wool, hair, and nail. Keratin is useful as natural protein. We developed the keratin loaded poly(L-lactide-co-glycolide) (PLGA) scaffolds (keratin/PLGA) for the possibility of the application of the tissue engineering using bone marrow mesenchymal (BMSCs). Keratin/PLGA (contents 0%, 10%, 20% and 50% of PLGA weight) scaffolds were prepared by solvent casting/salt leaching method. We characterized porosity, wettability, and water uptake ability, DSC of keratin/PLGA scaffold. We seeded BMSCs isolated from the femurs of rat into the inner core of the hybrid scaffold. Celluar viability were assayed by 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolium bromide (MTT) test. We confirmed that keratin/PLGA scaffold is hydrophilic by wettability, and water uptake ability measurement results. In MTT assay results, cell viability in scaffolds impregnated 10 and 20 wt% of keratin were higher than other scaffolds. In conclusion, we suggest that keratin/PLGA scaffold may be useful to tissue engineering using BMSCs.

• Food Science and Preservation
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• v.15 no.5
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• pp.736-742
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• 2008

To evaluate changes in functional characteristics of traditional Doenjang during aging, Doenjang was prepared using an extract of Phellinus linteus (Phellinus extract). Control Doenjang was aged without the extract. The protease activity of Doenjang prepared with Phellinus extract was 3.15 units/mL. Tyrosinase and acetylcholinesterase inhibition activities were 45.78% and 55.18% of control, respectively, in the treated sample. When Salmonella enterica serovar Typhimurium TA100 was used as a reporter strain, antimutagenic activities against the mutagens MNNG and NPD were 90.42% and 82.57% of control values in the treated sample. When S. enterica serovar Typhimurium TA98 was used, antimutagenic activities were 60.28% and 50.33% of control, respectively. Hydrogen-donating activity was 86.65% in the treated sample, which was higher than that of the control (61.69%). Daidzin (an isoflavon glucoside) levels in Doenjang prepared with Phellinus extract were higher, by 35.49 mg/kg, than the control, whereas genistin was not detected in either group. Daidzin and genistin aglycone levels were 263.01 mg/kg and 262.60 mg/kg in the control and test groups, respectively.

• Korean Journal of Breeding Science
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• v.43 no.6
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• pp.559-563
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• 2011

Cymbidium 'Purple Star' (Miake 'Pieta' ${\times}$ 'Allstar Mariane') was developed from a cross between hybrids at the National Institute of Horticultural & Herbal Science (NIHHS), Rural Development Administration (RDA) in 2008. A cross was made between purple colored flower C. Miake 'Pieta' as maternal line and dark pink colored flower, C. 'Allstar Mariane' as paternal line in 1995. The seed germination, cultivation, selection, and characteristic trials were conducted from 1996 to 2002. The line was named as Wongyo 'F1-21' and phenotype was characterized in 2007 as a new cultivar. The 'Purple Star' has dark purple basal color (RHS, GP186A) on both of sepal and petal with red lip (RHS, RP59A). 'Purple Star' has about 10.7 flowers per flower stalk and flower size of 7.6 cm. General appearance of petals and sepals is slightly incurved shape. The plant size is intermediate having erect peduncle. Blooming is started from the late of January (mid-winter) under optimal culture condition. Leaf attitude and twisting is erect and weak respectively. This hybrid has attractive floral arrangement, long flower stalk (78.4 cm) and vigorous growth. 'Purple Star' has a great potential for exporting to Chinese market.

• Korean Journal of Breeding Science
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• v.42 no.6
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• pp.654-658
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• 2010

A FA (Lilium formolongi $\times$ Lilium asiatic hybrid) intersectional hybrid lily cultivar 'Orange Crown' was developed in 2007 at National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA) of Korea. The cross and immature embryo rescue was conducted between female parents 'Migreen' (Lilium formolongi 'Raizan' $\times$ Lilium asiatic hybrid 'A61') and male parent 'A01-187' (L. asiatic 'A96-28' $\times$ 'Sanzio') by cut style pollination method at Suwon in 2001. It was preliminarily selected as 'FA04-27' in 2004. Multiplication, bulb production and characteristic tests were conducted from 2004 to 2007. The evaluation of characteristics and preference were surveyed at a lily flower show of NIHHS at Taean in 2007. 'Orange Crown' flowers in the late of June and grows more than 183 cm stem length. Flowers bloom upward-facing, a little spotted and orange (RHS, O24A) petals. The pollen of 'Orange Crown' was sterile.

• Korean Journal of Breeding Science
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• v.43 no.3
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• pp.214-217
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• 2011

A light pink double freesia (Freesia hybrida Hort.) 'Pink Ribbon' was developed from a cross of breeding a line, 'Pink Jewel' derived 'Michelle' and 'Rossini' and 'Purple Rain' followed by seedling and line selections at the National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA) in 2008. Growth and flowering characteristic tests were conducted from 2004 to 2007. 'Pink Ribbon' has double type flower with light pink color (RHS, RP66C). It has simultaneous flowering of many florets, good flower shape, upright leaves and stalks. The average cut flower yield of 'Purple Rain' observed in the greenhouse from 2005 to 2007 was 7.7 cuttings per plant. It has good production of cormlets, vigorous growth, too. 'Purple Rain' could be cultured under greenhouse conditions in Korea.

• Korean Journal of Breeding Science
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• v.43 no.3
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• pp.210-213
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• 2011

A dark pink semi-double freesia (Freesia hybrida Hort.) 'Happy Birthday' was bred from a cross between 'Pink Jewel', F1 progeny of 'Michelle' and 'Rossini' as a female and 'White Lace' as a male parent. Test for the growth vigor and flowering characteristic were conducted from 2002 to 2007. A new variety 'Happy Birthday' has semi-double flower type with dark pink color (RHS color chart #RP66C). It has multiple flowers, distinctive flower shape, upright leaves and also stalks. The average yield of this variety 'Happy Birthday' counted over 6.7 stems per plant per season in the standard greenhouse cultivation method. It has also good production of cormlets, vigorous growth. A new variety 'Happy Birthday' is suitable for the greenhouse conditions during winter season in Korea.

• Korean Journal of Breeding Science
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• v.41 no.2
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• pp.145-148
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• 2009

Phalaenopsis (Phal.) "Yellow Dream" was developed by the National Institute of Horticultural & Herbal Science (NIHHS), Rural Development Administration (RDA) in 2005. This hybrid came from the progenies of a cross between Phal. "3031" which has yellow colored petals and small plant size, and Phal. "Golden Emperor" which has yellow colored petals with red spots and small plant size in 1995. "Yellow Dream" was finally selected in 2005 after the investigation of the growth and flower characteristics from 2000 to 2004. "Yellow Dream" had a yellow (Y4D) base color and light orange stripe petals (ORN34A) with a red lip (R42D). The average leaf length and flower width of "Yellow Dream" were 21cm and 6.8cm, respectively. It has a half erect leaf form and is a fast growing cultivar. This hybrid is relatively easy to make clones.

• Korean Journal of Medicinal Crop Science
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• v.27 no.2
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• pp.96-107
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• 2019

Background: The aim of this study was to compare the fatty acid composition, antioxidant activity, and the content of two major phenolic acids, caffeic and rosmarinic acids, of 45 Perilla accessions collected from Russia. Methods and Results: A total of 45 accessions of the genus Perilla were used in this study. The antioxidant activities of these accessions were analyzed using a spectrophotometer, and their caffeic and rosmarinic acid contents were determined using a reversed-phase ultraperformance liquid chromatography (UPLC) system. The Perilla seed oil was recovered using hexane in a soxhlet extraction method. The fatty acid compositions were analyzed using a Shimadzu QP2010 gas chromatography-mass spectoroscopy system. The results showed that accessions IT226732 and IT274300 had the highest content of caffeic and rosmarinic acid, respectively. A cluster analysis based on antioxidant assay results and concentration of phenolic acids led to the categorization of Perilla accessions into three major groups. The accessions in Group I were characterized by higher caffeic and rosmarinic acid content and antioxidant activity, compared to the accessions in the other two groups (p < 0.05). The total oil content ranged between 28.39 and 46.89%. The compositions of oleic, linoleic, and linolenic acid ranged from 11.83 to 19.55%, 11.92 to 16.71%, and 59.19 to 67.28%, respectively. Conclusions: The results of this study indicated that accessions IT274300, IT226732, IT274293, IT235818, and IT235820 could be used as a source of functional materials.