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A Study on Proliferation and Phenotypical Stability of Schwann Cell on Keratin/PLGA Film  

Oh, A-Young (Polymer Fusion Research Center, Chonbuk National University)
Kim, Soon-Hee (Polymer Fusion Research Center, Chonbuk National University)
Kim, Yun-Tae (Polymer Fusion Research Center, Chonbuk National University)
Jeon, Na-Ri (Polymer Fusion Research Center, Chonbuk National University)
Yang, Jae-Chan (Polymer Fusion Research Center, Chonbuk National University)
Lee, Sang-Jin (Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Medical Center Boulevard)
Yoo, James-J. (Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Medical Center Boulevard)
Van Dyke, Mark (Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Medical Center Boulevard)
Shin, Hyung-Sik (Division of Chemical Engineering, Chonbuk National University)
Rhee, John-M. (Polymer Fusion Research Center, Chonbuk National University)
Khang, Gil-Son (Polymer Fusion Research Center, Chonbuk National University)
Publication Information
Polymer(Korea) / v.33, no.2, 2009 , pp. 118-123 More about this Journal
Abstract
Keratin contains regulatory molecules that can enhance neuronal cell activity. We fabricated keratin/ PLGA films using 0, 10, 20, and 50 wt% of keratin using solvent casting method. We measured the contact angle of each film and cell proliferation was assayed by counting the cells attached on the film. Adhered cell morphology was confirmed by scanning electron microscope. RT-PCR was conducted to evaluate the gene expression of NF, NSE, and S-100, the Schwann cell markers. The keratin content of 20 and 50 wt% provided higher wettability than PLGA. The 20 wt% keratin was better in cell adhesion and proliferation of SCs than other keratin/PLGA films. The phenotypic stability of SC was maintained with the keratin content of 10 and 20 wt%.
Keywords
keratin; PLGA; phenotype; Schwann cell; proliferation;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
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