• Korean Journal of Environmental Biology
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• v.22 no.1
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• pp.206-212
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• 2004

In an effort to develop the tools for monitoring the contamination of xenoestrogen in the aquatic environment of Korea, reverse transcription-polymerase chain reaction (RT-PCR) analysis of vitellogenin (VTG) mRNA expression were optimized in Synechogobius hastus. Based on the partial VTG cDNA sequence VTG mRNA level in livers from male fishes was analyzed by RT-PCR. As an internal control beta actin mRNA was amplified. 3 ${\mu}g$ of total RNA was reverse transcribed in 20 $\mu$l reaction using murine leukemia virus 〔MuLV〕 reverse transcriptase. Subsequent PCR using the 1 ${\mu}g$ of cDNA resulted in linear increase in PCR product of VTG in female liver cDNA from 10 to 30 cycles of amplification. On the contrary, in male, PCR product first detected at 28 cycles of amplification and linearly increased during 38 cycles of amplification, suggesting that male S. hastus expresses minute amount of VTG mRNA which is $2^{-18}$ equivalent of female. In conclusion, the optimized protocol of VTG mRNA expression in the liver of male S. hastus will be promising the environmental monitoring the xenoestrogen contamination in the western coast and estuaries in Korea.

• Journal of the Institute of Convergence Signal Processing
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• v.2 no.2
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• pp.55-61
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• 2001

In this paper, a synchronous reverse link DS-CDMA system with cdma2000 standard is simulated to analyze performance of system and to find the optimum power ratio between pilot channel and traffic channel. In the cdma2000 the pilot channel is used to estimate fading channel, in contract to IS-95 and it compensates phase variation of received signal due to channel environment. When the length of channel estimation is 31pcg symbols, we can find the optimum power ratio of pilot channel to traffic channel. And also we find Eb/No satisfying target FER when the power control is applied and analyze performance of system with multiuser transmission.

• Preventive Nutrition and Food Science
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• v.18 no.2
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• pp.111-116
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• 2013

Chrysanthemum indicum is widely used to treat immune-related and infectious disorders in East Asia. C. indicum flower oil contains 1,8-cineole, germacrene D, camphor, ${\alpha}$-cadinol, camphene, pinocarvone, ${\beta}$-caryophyllene, 3-cyclohexen- 1-ol, and ${\gamma}$-curcumene. We evaluated the safety of C. indicum flower oil by conducting acute oral toxicity, bone marrow micronucleus, and bacterial reverse mutation tests. Mortality, clinical signs and gross findings of mice were measured for 15 days after the oral single gavage administration of C. indicum flower oil. There were no mortality and clinical signs of toxicity at 2,000 mg/kg body weight/day of C. indicum flower oil throughout the 15 day period. Micronucleated erythrocyte cell counts for all treated groups were not significantly different between test and control groups. Levels of 15.63~500 ${\mu}g$ C. indicum flower oil/plate did not induce mutagenicity in S. Typhimurium and E. coli, with or without the introduction of a metabolic activation system. These results indicate that ingesting C. indicum flower oil produces no acute oral toxicity, bone marrow micronucleus, and bacterial reverse mutation.

• KSBB Journal
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• v.8 no.1
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• pp.83-88
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• 1993

Lipase was separated using reverse mlcelles in a spray column. The 50 mM AOT-Isooctane solution was used as reverse micellar solution for the extraction of lipase (crude containing 25% Protein). Ionic strength was controlled by KCl(0.1M KCl for extraction, 0.5M KCl for back exlractlon). Acetate buffer and phosphate buffer were used for control of pH. The efficiencies of extraction and stripping were 30% and 50%. An increase of circulation did not change the efficiency of extraction in forward extraction. The optimum flow rate was around 0.10ml/sec.

• Clinics in Shoulder and Elbow
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• v.24 no.3
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• pp.141-146
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• 2021

Background: The purpose of the present study was to determine how long superior screws alone or in combination with posterior placement of metaglene screws protruding and penetrating into the scapular spine in reverse total shoulder arthroplasty affect the strength of the scapular spine in a fresh cadaveric scapular model. Methods: Seven fresh cadaver scapulas were allocated to the control group (short posterior and superior screws) and seven scapulars to the study group (spine base fixation with a four long screws, three with both long superior and long posterior screws). Results: The failure load was lower in the spine fixation group (long screw, 869 N vs. short screw, 1,123 N); however, this difference did not reach statistical significance (p>0.05). All outside-in long superior or superior plus posterior screws failed due to scapular spine base fracture; failures in the short screw group were due to acromion fracture. An additional posterior outside-in screw failed to significantly decrease the failure load of the acromion spine. Conclusions: The present study highlights the significance of preventing a cortical breach or an outside-in configuration when a superior or posterior screw is inserted into the scapular spine base.

• Journal of the Korean Institute of Telematics and Electronics S
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• v.35S no.7
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• pp.22-28
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• 1998

This paper includes AC servo motor speed control usig the predictive control strategy. Generally, AC servo motor control should have the fast response characteristics. For the issue, sliding mode control and PID control have been applied. However, the former has the speed ripple response due to the chattering and the latter requires the many trial efforts. Originally, the predictive control which has been used in process control area does not need the priori knowledge for the application system and it is easy to compute the optimal gain with the prediction. In this paper, the TMS320C31 DSP pocessor is used for AC motor control with fst dynamics and the tuning guid-line for the parameters of the predictive control algorithm is given in order to reduce the computation load. Also, the actuator saturationis implemented uisngthe QP(Quadratic Programming) method and the transient response is improved by the identified intertia coefficient when AC motor is drived at forward/reverse rotation.

• Korean Journal of Medicinal Crop Science
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• v.9 no.1
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• pp.21-25
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• 2001

Major saponins in ginseng were analysed using reverse phase high performance liquid chromatography with binary mobile phase gradient control system instead of normal phase column. The optimum condition were as following : reverse phase column; ${\mu}{\beta}ondapak\;C_{18}$ column (Waters, $3.9mm{\times}300\;mm,\;5{\mu}m$), methyl cyanaide/water binary mobile phase gradient control system, solvent flow rate; 1.5 ml/min, and UV($203{\mu}m$ ) detector. The complete separation of ginsenoside $Rb_1,\;Rb_2,\;Rc,\;Rd,\;Re,\;Rf\;and\;Rg_1$ was achieved within 55 min. The Regression coefficients of the calibration curves for seven ginsenosides were 0.99.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.14 no.4
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• pp.313-322
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• 2003

In this paper, the reverse link performance of imperfect power controlled cdma2000 cellular system that using array at a base station is analyzed, when MCGM beamforming algorithm and power control error is considered in shadowing. The blocking probability of the cdma2000 cellular system based on array parameters, E$\_$b//N$\_$0/ and interference statistics is calculated, and then the system capacity is calculated at a specific blocking probability. When the blocking probability is set 1 %, PCE(power control error) is 2 dB, M=2, 4, 8, 10, the capacity of cdma2000 is increased 2.3 ∼ 2.5 times higher than IS-95.

• Journal of Veterinary Science
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• v.21 no.2
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• pp.24.1-24.11
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• 2020

The pandemic of avian influenza viruses (AIVs) in Asia has caused enormous economic loss in poultry industry and human health threat, especially clade 2.3.4.4 H5 and H7 subtypes in recent years. The endemic chicken H6 virus in Taiwan has also brought about human and dog infections. Since wild waterfowls is the major AIV reservoir, it is important to monitor the diversified subtypes in wildfowl flocks in early stage to prevent viral reassortment and transmission. To develop a more efficient and sensitive approach is a key issue in epidemic control. In this study, we integrate multiplex reverse transcription recombinase polymerase amplification (RT-RPA) and capillary electrophoresis (CE) for high-throughput detection and differentiation of AIVs in wild waterfowls in Taiwan. Four viral genes were detected simultaneously, including nucleoprotein (NP) gene of all AIVs, hemagglutinin (HA) gene of clade 2.3.4.4 H5, H6 and H7 subtypes. The detection limit of the developed detection system could achieve as low as one copy number for each of the four viral gene targets. Sixty wild waterfowl field samples were tested and all of the four gene signals were unambiguously identified within 6 h, including the initial sample processing and the final CE data analysis. The results indicated that multiplex RT-RPA combined with CE was an excellent alternative for instant simultaneous AIV detection and subtype differentiation. The high efficiency and sensitivity of the proposed method could greatly assist in wild bird monitoring and epidemic control of poultry.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 2005.05a
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• pp.228-230
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• 2005

Magnetic field has been used to hold plasma at high temperature for a long time. Reverse field has shown unstable process. Using ratio frequency, the author could control the instability of the process and formed a stable erversed field.