• Journal of fish pathology
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• v.10 no.1
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• pp.39-44
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• 1997

Metallothionein is an important and essential protein to control the intracellular concentration of heavy metals, which exist in all organisms from bacteria to vertebrates. Although the detailed functions and induction mechanisms of metallothionein gene have not been clearly characterized until yet, the structure of several metallothionein genes has been revealed. Especially, piscine metallothionein is regarded as an important protein because it is induced by several heavy metal pollutants and environmental stress and it could be determined the comparative amount of heavy metals and the extent of environmental stress by assaying the RNA transcript of metallothionein gene in the method of the quantitative RT-PCR(Reverse Transcriptase Polymerase Chain Reaction). In this study I have characterized the 450 bp PCR fragment of metallothionein gene amplified by using the mixture of internal specific primers and universal 3' end primer. The nucleotide sequence analysis of 450 bp PCR fragment amplified in cDNA library of channel catfish did not show strong homology to other piscine metallothionein genes.

• Journal of Korean Society for Atmospheric Environment
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• v.16 no.3
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• pp.237-246
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• 2000

An experimental study has been carried out using a rotating water channel in order to investigate the effect of surface roughness on the vertical dispersion of plume within boundary layer. Dispersion measurements of tracers released from two sources with different height at neutral conditions over various rough terrain ranging from rural to urban have been performed. Various values of roughness length were simulated by combining of 4 stream velocities and 3 roughness element conditions. Dispersion measurements have also been made for rough terrain where high buildings are locally concentrated. Values of $\sigma$z increase with roughness and this tendency appears to apply both cases of with and without locally concentrated high buildings. The comparisons of the Bowne's nomogram on $\sigma$2 vs x relationship and the measurements of $\sigma$2 with roughness show good accordance in $\sigma$2 distribution at stability D class over rural, suburban and urban terrain. For constant roughness length the $\sigma$2 values of plumes from lower source height are smaller than those of plumes from higher source at short downwind distance, but this relationship becomes reverse as distance increases. Crossing appears to be made before about 2km. The value of constant I in McMullen's equation $\sigma$2=exp [I+J(In x) + K(In x)2] appears to increase with roughness length, however, the relationships between other constants and roughness have been confirmed. The values of $\sigma$2 for various downwind distances, estimated by using an equation which is employed in ISC (Industrial Source Complex) dispersion model for areas where high buildings are locally assembled, are in accordance with measurements from water channel experiments.

• The Korean Journal of Physiology and Pharmacology
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• v.15 no.6
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• pp.371-382
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• 2011

We performed experiments using Aplysia neurons to identify the mechanism underlying the changes in the firing patterns in response to temperature changes. When the temperature was gradually increased from $11^{\circ}C$ to $31^{\circ}C$ the firing patterns changed sequentially from the silent state to beating, doublets, beating-chaos, bursting-chaos, square-wave bursting, and bursting-oscillation patterns. When the temperature was decreased over the same temperature range, these sequential changes in the firing patterns reappeared in reverse order. To simulate this entire range of spiking patterns we modified nonlinear differential equations that Chay and Lee made using temperature-dependent scaling factors. To refine the equations, we also analyzed the spike pattern changes in the presence of potassium channel blockers. Based on the solutions of these equations and potassium channel blocker experiments, we found that, as temperature increases, the maximum value of the potassium channel relaxation time constant, ${\tau}_n(t)$ increases, but the maximum value of the probabilities of openings for activation of the potassium channels, n(t) decreases. Accordingly, the voltage-dependent potassium current is likely to play a leading role in the temperature-dependent changes in the firing patterns in Aplysia neurons.

• Bulletin of the Korean Chemical Society
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• v.32 no.2
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• pp.510-514
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• 2011

Photodissociation dynamics of allyl alcohol ($H_2C$=CH-$CH_2OH$) has been investigated at 205 - 213 nm along the UV absorption band by measuring rotationally-resolved laser-induced fluorescence spectra of OH radicals. Observed energy partitioning of the available energy among products at all photon energies investigated was similar and the barrier energy for OH production is about 574.7 kJ/mol from the OH yield measurements. The potential energy surfaces for the $S_0$, $T_1$, and $S_1$ excited states along the dissociation coordinate were obtained by ab initio quantum chemical calculations. The observed energy partitioning was successfully modeled by the "barrier-impulsive model" with the reverse barrier and the geometry obtained by the calculated potential energy surfaces. The dissociation takes place on the $T_1$ excited state potential energy surface with an energy barrier in the exit channel and a large portion of the photon energy is distributed in the internal degrees of freedom of the polyatomic products.

• Journal of the Institute of Electronics Engineers of Korea TE
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• v.37 no.2
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• pp.54-61
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• 2000

This paper presents the performance evaluation of channel estimation algorithm using RLS algorithm lot W-CDMA reverse link over Rayleigh fading channels. By obtaining BER(Bit Error Rate) performance through computer simulations, the RLS(Recursive Least Square) algorithm is compared with the existing WMSA(Weighted Averaging)(K=1,3) and constant gain algorithm. The channel structure, modulation and pilot patterns are applied to the ARIB (Association of Radio Industries and Business) and 3GPP (3rd Generation Partnership Project) ITU-R proposal for the IMT-2000. The BER performance of RLS algorithm with linear interpolation is similar to that of WMSA(K=1) and slightly superior to that of constant gain algorithm at low Doppler frequencies. Also, RLS algorithm performance is better than that of the WMSA(K=1,3) and constant gain algorithms about 4dB at BER=2.0$\times$$10^{-2}$ and Doppler frequencies $F_D$=320Hz. With increasing Doppler frequency, therefore, the BER performance of RLS algorithm with linear interpolation is superior to WMSA(K=L.3) and constant rain algorithms.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.8B
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• pp.745-751
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• 2002

The performance of existing CDMA-type multiple access data transmission systems is limited by interferences due to adverse mobile communication channel characteristics. Among them, the multi-user interference becomes one of the major performance degradation factors because the signal-to-signal orthogonality can be easily perturbed when numerous user signals are distorted by multipath fading channels and mixed together. In order to enhance the performance of CDMA-type systems by suppressing the multi-user interference, we have adopted chip-based cyclic prefix insertion along with adaptive one-tap DFE equalization into MC-DS-CDMA, which is known for its robustness in the frequency selective multipath fading channel environment. In order to assess the performance of the proposed system, a set of computer simulations is performed in the reverse link in which each user signal undergoes different multipath Rayleigh fading. The results show us a superior performance of our system over other CDMA systems in terms of SNR to BER measurements.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.23 no.7
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• pp.1677-1688
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• 1998

In this paper, we analyze the performance of a DS-CDMA system employing adaptive adaptive array antenna technology at base-station. For the analysis the receiving architecture of a multiple team adaptive base-station array antenna is proposed as a major system omponent, and signal to interference and noise ratio(SINR) on the proposed architecture is developed. We show that the perfomance enhancement of the CDMA system is represented as great reduction of interfreence by employing an array antenna capable of resolving the angular distribution of the mobile users as seen at the ase-station. The energy associated with each mobile or a group of mobiles is thus confined within the addressed volume, greatly reducing the amount of co-channel interference experienced from and by neighboring co-channel cells. In order to ascertain the benefits of such an antenna, a theoretical approach, which eis based on the conventional and proposed antenna systems in a typical mobile radio environment, is adopted. Finally computer simulation results show the insight into how the adaptive array antenna operates when used in conjunction with DS-CDMA and illustrate the potential benefits in the SINR point of view.

• Animal cells and systems
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• v.1 no.4
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• pp.589-594
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• 1997

The neuronal voltage-sensitive calcium channels (VSCCs) are multisubunit complexes consisting of $\alpha_1,\;\alpha_2-\delta$ and $\beta$ subunits. Heterologous expression and biochemical studies have shown that the activity of VSCCs is regulated by their $\beta$ subunits in a $\beta$ subunit isoform-specific manner. To elucidate the $\beta$ subunit identity of the P/Q-type calcium channel encoded by an $\alpha_{1A}$ subunit, which is exclusively expressed in the Purkinje and granule cell of the cerebellum, we have examined the spatial and temporal expression patterns of $\beta$ subunits and compared them with those of $\alpha_{1A}$ subunit in the developing rat cerebellum. Reverse transcriptase- polymerase chain reaction (RT-PCR) and Northern blot analysis have shown that $\beta_4$ subunit mRNA was prominently expressed in the cerebellum and much more abundant than any other distinct $\beta$ subunits. RNase protection assay has further demonstrated that the expression of $\alpha_{1A}$ and $\beta_4$ subunits increased during cerebellar development, while the amount of $\beta_2$ and $\beta_3$ mRNAs did not significantly change. In addition, a $\beta_4$ transcript was present in cultured cerebellar granule cells, but not in astrocyte cells, and the level of $\beta_4$ mRNA expression increased gradually in vitro seen as in vivo. Based on the spatial and temporal expression patterns of $\beta_4$ subunit, we conclude that $\beta_4$ may predominantly associate, but probably not exclusively, with the $\alpha_{1A}$ subunit in rat cerebellar granule cells.

• Journal of IKEEE
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• v.5 no.1 s.8
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• pp.1-7
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• 2001

In this paper, we have investigated the CDMA(Code Division Multiple Access) Cellular System with non-linear equalizer in reverse link channel. In general, due to unknown characteristics of channel in the wireless communication, the distribution of the observables cannot be specified by a finite set of parameters; instead, we partitioned the m-dimensional sample space Into a finite number of disjointed regions by using quantiles and a vector quantizer based on training samples. The algorithm proposed is based on a piecewise approximation to regression function based on quantiles and conditional partition moments which are estimated by Robbins Monro Stochastic Approximation (RMSA) algorithm. The resulting equalizers and detectors are robust in the sense that they are insensitive to variations in noise distributions. The main idea is that the robust equalizers and robust partition detectors yield better performance in equiprobably partitioned subspace of observations than the conventional equalizer in unpartitioned observation space under any condition. And also, we apply this idea to the CDMA system and analyze the BER performance.

• The Korean Journal of Physiology and Pharmacology
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• v.12 no.5
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• pp.267-274
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• 2008

Since first discovered in chick skeletal muscles, stretch-activated channels (SACs) have been proposed as a probable mechano-transducer of the mechanical stimulus at the cellular level. Channel properties have been studied in both the single-channel and the whole-cell level. There is growing evidence to indicate that major stretch-induced changes in electrical activity are mediated by activation of these channels. We aimed to investigate the mechanism of stretch-induced automaticity by exploiting a recent mathematical model of rat atrial myocytes which had been established to reproduce cellular activities such as the action potential, $Ca^{2+}$ transients, and contractile force. The incorporation of SACs into the mathematical model, based on experimental results, successfully reproduced the repetitive firing of spontaneous action potentials by stretch. The induced automaticity was composed of two phases. The early phase was driven by increased background conductance of voltage-gated $Na^+$ channel, whereas the later phase was driven by the reverse-mode operation of $Na^+/Ca^{2+}$ exchange current secondary to the accumulation of $Na^+$ and $Ca^{2+}$ through SACs. These results of simulation successfully demonstrate how the SACs can induce automaticity in a single atrial myocyte which may act as a focus to initiate and maintain atrial fibrillation in concert with other arrhythmogenic changes in the heart.