Drought stress is one of the important factors that restrict the expansion of Hevea brasiliensis cultivation to non-traditional regions experiencing extreme weather conditions. Plants respond to drought stress by triggering expression of several drought responsive genes including transcription factors which in turn trigger expression of various downstream signalling pathways and adaptive networks. Expression of such drought responsive genes may revert back to their original level upon re-watering. However, no reports are available on such phenomenon in Hevea and hence, this study was initiated. For this purpose, NAC transcription factor (NAC tf) was chosen as candidate gene. Its expression levels were monitored under intermittent drought as well as irrigated conditions in two clones (RRII 105 and RRIM 600) of H. brasiliensis with contrasting tolerance level. Copy number of NAC tf was found similar in both the clones. Expression of NAC tf was found highly up-regulated in RRIM 600 (a relatively drought tolerant clone) than in RRII 105 (a relatively drought susceptible clone) throughout the drought incidences which upon re-watering, reached back to its original levels in both the clones. The study indicated the existence of an association between expression of NAC tf and drought tolerance trait exhibited by the tolerant clone RRIM 600. The study also proves the influence of drought and re-watering on the leaf photosynthesis and expression of NAC tf in H. brasiliensis.
Journal of The Korean Society of Grassland and Forage Science
/
v.38
no.3
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pp.190-195
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2018
Cold, salt and heat are the most critical factors that restrict full genetic potential, growth and development of crops globally. However, clarification of genes expression and regulation is a fundamental approach to understanding the adaptive response of plants under unfavorable environments. In this study, we applied an annealing control primer (ACP) based on the GeneFishing approach to identify differentially expressed genes (DEGs) in Italian ryegrass (cv. Kowinearly) leaves under cold, salt and heat stresses. Two-week-old seedlings were exposed to cold ($4^{\circ}C$), salt (NaCl 200 mM) and heat ($42^{\circ}C$) treatments for six hours. A total 8 differentially expressed genes were isolated from ryegrass leaves. These genes were sequenced then identified and validated using the National Center for Biotechnology Information (NCBI) database. We identified several promising genes encoding light harvesting chlorophyll a/b binding protein, alpha-glactosidase b, chromosome 3B, elongation factor 1-alpha, FLbaf106f03, Lolium multiflorum plastid, complete genome, translation initiation factor SUI1, and glyceraldehyde-3-phosphate dehydrogenase. These genes were potentially involved in photosynthesis, plant development, protein synthesis and abiotic stress tolerance in plants. However, this study provides new insight regarding molecular information about several genes in response to multiple abiotic stresses. Additionally, these genes may be useful for enhancement of abiotic stress tolerance in fodder crops as well a crop improvement under unfavorable environmental conditions.
The purpose of this study is to identify the factors influencing the quality of life and to compare the changes in quality of life by each period group in workers with diabetes mellitus. A secondary data analysis was conducted using the data of "The Korea National Health and Nutrition Examination Survey, 2007-2015." The survey included 1141 worker with diabetes mellitus from 19 to 65. Data analysis was conducted using SPSS 23.0 program and descriptive statistics, ANOVA, and multiple regression analyses were performed. Factors influencing quality of life are gender, household income level, subject health state, restrict activity, sleeping hours, stress. Subject health state, restrict activity, stress were common factors in the 4th, 5th, and 6th period group. The difference in each group were factors influencing the quality of life, such as sleep time(4th), gender(5th), Household income level and diagnosis of angina pectoris(6th). This study can be used to convergence study the diabetic management program and social role for improving the quality of life of wrkers with diabetes Mellitus.
Seo, Hyung-Joon;Kim, Hyun-Rae;Jeong, Nam-Soo;Lee, In-Mo
Proceedings of the Korean Geotechical Society Conference
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2010.03a
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pp.523-534
/
2010
A hybrid system of soil-nailing and compression anchor is proposed in this paper; the system is composed of an anchor bar (installed at the tip) with two PC strands and a steel bar. After drilling a hole, installing proposed hybrid systems, and filling the hole with grouting material, prestress is applied to the anchor bar to restrict the deformation at the head and/or to prevent shallow slope failures. However, since the elongation rate of PC strand is much larger than that of steel bar, yield at the steel bar will occur much earlier than the PC strand. It means that the yield load of the hybrid system will be overestimated if we simply add yield loads of the two - anchor bar and PC strands. It might be needed to try to match the yielding time of the two materials by applying the prestress to the anchor bar. It means that the main purpose of applying prestress to the anchor bar should be two-fold: to restrict the deformation at the nail head; and more importantly, to maximize the design load of the hybrid system by utilizing load transfer mechanism that transfers the prestress applied at the tip to the head through anchor bar. In order to study the load transfer mechanism in a systematic way, in-situ pullout tests were performed with the following conditions: soil-nailing only; hybrid system with the variation of prestress stresses from 0kN to 196kN. It was found that the prestress applied to the anchor system will induce the compressive stress to the steel bar; it will result in decrease in the slope of load-displacement curve of the steel bar. Then, the elongation at which the steel bar will reach yield stress might become similar to that of PC strands. By taking advantage of prestress to match elongations at yield, the pullout design load of the hybrid system can be increased up to twice that of the soil-nailing system.
Journal of the Korean Academy of Child and Adolescent Psychiatry
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v.3
no.1
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pp.106-116
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1992
This study were purposed to find out parental perceptions of child adjustment associated with parenting stress with autistic children and differences of parenting stress between autistic children's parents and normal children's. Subjects were 27 autistic children's parents, 28 normal children's, 4 teachers with autistic children. 'Scales were Parenting Stress Index(PSI), Eyberg Child Behavior Index(ECBI), Childhood Autism Rating Scale(CARS), Social Maturing Scale(SMS). The results of this study were as follows. 1) In total parenting stress, parents with autistic children had significantly higher scores than normal's. 2) In child domain of parenting stress, fathers with autistic children had significantly higher than mothers on the other components except Mood and Demandingness. 3) In parent domain of parenting stress, parents with autistic children had significantly higher than nomal's on Restrict of role, Attachment, Sense of Competence. All mothers of normal and autistic children had significantly higher than fathers on the other components except Repression. 4) In parenting perceptions associated with parenting stress level of autistic children's parents. Low level group perceived their children as higher adaptibility than teacher's evaluation and high level group perceived them as equal to teacher's.
This study examined the effect of rotational stiffness of joints between vertical and horizontal members in system supports. In order to prevent repeated disasters of system supports, it is important to examine the accurate behavior of system supports. Among various factors affecting the complex behavior of system supports, this study focused on the stiffness of joints between vertical and horizontal members. The considered joint was modelled by a rotational spring, but the translational displacements were fixed. The stiffness of rotational spring was calculated by utilizing the usable experimental data. In addition, the hinge connection condition, which is generally considered in design and only restrict the translational displacements, was modelled to compare the results. The case with the rotational stiffness in joints showed 3.5 times buckling loads compared to the case without the rotational stiffness. Thus, the structural behavior of the vertical member in system supports was similar to the vertical member with the fixed condition. For the combined stresses of vertical members, the combined stress ratios were reduced 5~6% by considering the rotational stiffness of connecting parts. However, for the horizontal member where showed relatively small stress range, the stresses were increased 2.3~7.6 times by considering the rotational stiffness in connecting parts.
Despite the therapeutic effect of mesenchymal stem cells (MSCs) in ischemic diseases, pathophysiological conditions, including hypoxia, limited nutrient availability, and oxidative stress restrict their potential. To address this issue, we investigated the effect of melatonin on the bioactivities of MSCs. Treatment of MSCs with melatonin increased the expression of peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α). Melatonin treatment enhanced mitochondrial oxidative phosphorylation in MSCs in a PGC-1α-dependent manner. Melatonin-mediated PGC-1α expression enhanced the proliferative potential of MSCs through regulation of cell cycle-associated protein activity. In addition, melatonin promoted the angiogenic ability of MSCs, including migration and invasion abilities and secretion of angiogenic cytokines by increasing PGC-1α expression. In a murine hindlimb ischemia model, the survival of transplanted melatonin-treated MSCs was significantly increased in the ischemic tissues, resulting in improvement of functional recovery, such as blood perfusion, limb salvage, neovascularization, and protection against necrosis and fibrosis. These findings indicate that the therapeutic effect of melatonin-treated MSCs in ischemic diseases is mediated via regulation of PGC-1α level. This study suggests that melatonin-induced PGC-1α might serve as a novel target for MSC-based therapy of ischemic diseases, and melatonin-treated MSCs could be used as an effective cell-based therapeutic option for patients with ischemic diseases.
Nowadays, the nutritional deficits are rarely seen in Korea. However, an increased availability of the highly palatable energy dense, nutrient-poor foods increases the risks of obesity and deficits of vitamins and minerals in the general population. Also, optimum intake of vitamins and minerals, which varies with age and genetic back ground, might not suffice the poor, young, obese, and elderly people. Young girls and individuals participating in weight reductions and aesthetic components are prone to micronutrient deficiencies because they restrict food intake and specific micronutrient rich foods. An inadequate intake of vitamins or minerals is associated with reduced physical performance and exercise capacity, increased obesity, decreased cognitive function, increased DNA damages such as single- and double-stranded breaks or oxidative DNA lesions, and accelerated aging process and increased neuronal damages with mitochondrial oxidative decay. Most of these deleterious effects of the deficit could be prevented by a one tablet of multivitamins with a good balanced diet. High dose B vitamins are frequently administered to overcome the metabolic inadequacy to the people with the less functional enzymes with increased Km values for their coenzymes due to the single gene mutation or due to the single nucleotide polymorphisms. And some certain antioxidant vitamins are also used in large quantities to overcome the oxidative stress and to repair the damages. In this review, new nutritional concepts of some vitamins and minerals, which are widely used and useful for the children, will be discussed.
Nakano, Satoshi;Kato, Chihiro;Purcell, Larry C.;Shiraiwa, Tatsuhiko
Proceedings of the Korean Society of Crop Science Conference
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2017.06a
/
pp.308-308
/
2017
The low and unstable yield of soybean has been a major problem in Japan. Excess soil moisture conditions are one of the major factors to restrict soybean productivity. More than 80 % of soybean crops are cultivated in converted paddy fields which often have poor drainage. In central and eastern regions of Japan, the early vegetative growth of soybean tends to be restricted by the flooding damage because the early growth period is overlapped with the rainy season. Field observation shows that induced excess water stress in early vegetative stage reduces dry matter production by decreasing intercepted radiation by leaf and radiation use efficiency (RUE) (Bajgain et al., 2015). Therefore, it is necessary to evaluate the responses of soybean growth for excess water conditions to assess these effects on soybean productions. In this study, we aim to modify the soybean crop model (Sinclair et al., 2003) by adding the components of the restriction of leaf area development and RUE for adaptable to excess water conditions. This model was consist of five components, phenological model, leaf area development model, dry matter production model, plant nitrogen model and soil water balance model. The model structures and parameters were estimated from the data obtained from the field experiment in Tsukuba. The excess water effects on the leaf area development were modeled with consideration of decrease of blanch emergence and individual leaf expansion as a function of temperature and ground water level from pot experiments. The nitrogen fixation and nitrogen absorption from soil were assumed to be inhibited by excess water stress and the RUE was assumed to be decreasing according to the decline of leaf nitrogen concentration. The results of the modified model were better agreement with the field observations of the induced excess water stress in paddy field. By coupling the crop model and the ground water level model, it may be possible to assess the impact of excess water conditions for soybean production quantitatively.
International joint ventures are usually formed and managed by domestic companies and foreign investors for the common objectives. They offer an opportunity for each partner to benefit significantly from the comparative advantages of the other. Local partners bring knowledge of the domestic market; familiarity with government bureaucracies and regulations; understanding of local labor markets; and existing manufacturing facilities. Foreign partners can offer advanced process and product technologies, management know-how, and access to export markets. In Korea, joint ventures have been encouraged to usher in foreign investors with foreign currency capital badly needed during the IMF financial crisis. In the meantime, Korean laws and regulations with respect to joint ventures have been largely overhauled to promote foreign direct investment (FDI) both inbound and outbound. They include four types of FDI, i.e., acquisition of foreign stocks, provision of long-term loans, participation in joint operations like resources development, and establishment of foreign offices. From the legal point of view, the formal joint venture agreement must be an offspring of a series of tough negotiations between domestic and foreign partners. They usually stress the long-term relationship with the good will and dedication to each other, and restrict the free transfer of stocks. Both partners are earnestly interested in the ownership and management of the joint venture. So they keep a close eye on the articles of incorporation, changes of business environment, conflict resolution methods, transparency of accounting and other financial matters. When a multinational corporation (MNC) is involved in the joint venture, conflicts over management strategies, marketing and other issues take place more often than not between the MNC and local partners. We have to pay attention to joint ventures, particularly, in China and North Korea. As witnessed in other transition economies, China is eagerly bringing in foreign direct investments for the development of nation's economy. China encourages foreign investors to establish ordinary joint ventures, contractual joint ventures, solely invested foreign capital companies and jointly operated development companies with local partners. In North Korea, however, joint ventures have a different meaning like contractual joint ventures in China, in which North Korean partners have an initiative in the management. Rather, jointly operated companies or simply processing-for-wage companies are recommended in view of the unpredictable legal infrastructure in North Korea.
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