• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.9-9
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• 2021

Mutation breeding is useful for improving agronomic characteristics of various crops. In this study, we constructed soybean Mutant Diversity Pool (MDP) from 1,695 gamma-irradiated mutants through two selection phases over M1 to M12 generations; we selected 523 mutant lines exhibiting at least 30% superior agricultural characteristics, and, second, we eliminated redundant morphological phenotypes in the M12 generation. Finally, we constructed 208 MDP lines and investigated 11 agronomic traits. We then assessed the genetic diversity and inter-relationships of these MDP lines using target region amplification polymorphism (TRAP) markers. Among the different TRAP primer combinations, polymorphism levels and PIC values averaged 59.71% and 0.15, respectively. Dendrogram and population structure analyses divided the MDP lines into four major groups. According to an analysis of AMOVA, the percentage of inter-population variation among mutants was 11.320 (20.6%), whereas mutant inter-population variation ranged from 0.231 (0.4%) to 14.324 (26.1%). Overall, the genetic similarity of each cultivar and its mutants were higher than within other mutant populations. In an analysis of the genome-wide association study (GWAS) using based on the genotyping-by-sequencing (GBS), we detected 66 SNPs located on 13 different chromosomes were found to be highly associated with four agronomic traits: days of flowering (33 SNPs), flower color (16 SNPs), node number (6 SNPs), and seed coat color (11 SNPs). These results are consistent with those previously reported for other genetic resource populations, including natural accessions and recombinant inbred line. Our observations suggest that genomic changes in mutant individuals induced by gamma rays occurred at the same loci as those of natural soybean population. This study has demonstrated that the integration of GBS and GWAS can serve as a powerful complementary approach to gamma-ray mutation for the dissection of complex traits in soybean.

• Fisheries and Aquatic Sciences
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• v.27 no.7
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• pp.421-433
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• 2024

Among larger species of fish found in Punatsang chhu, Brown trout (Salmo trutta) and Snow trout (Schizothorax richardsonii) are notable species. Snow trout is a native species, whereas Brown trout is an introduced species. Brown trout is noted to have a negative impact on the population of native species worldwide. This study studied the population dynamics and structure of these species to detect if the introduced species affected the native species adversely. The study was conducted on a 30 km stretch of Punatsang chhu including its major and minor tributaries at about their confluences. In total, 56 sample plots of a 200 m sampling stretch were studied with a 200 m distance between each stretch. Length, weight, maturity, and sex data for both species were collected along with the habitat and water parameters. The two species were found to prefer different areas within a single stretch with a rare overlap. Snow trout were found to be distributed widely within the basin whereas Brown trout were only found in altitudes higher than 1,000 masl ($\overline{x}$ = 1,231.77) and in turbid waters (p = 0.009). Recruitment of Snow trout was not good whereas recruitment of Brown trout was good. Length-frequency graphs indicated good inverse J distribution for the Snow trout population and sporadic for Brown trout. There is an effect of Brown trout introduction to the Snow trout living in the study area. Places invaded by Brown trout have a lesser presence of Snow trout compared to places not invaded by these species.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.2
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• pp.559-564
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• 2015

Background: The participation of women in cervical cancer screening in Malaysia is low. Self-sampling might be able to overcome this problem. The aim of this study was to assess the reliability of self-sampling for cervical smear in our country. Materials and Methods: This cross-sectional study was conducted on 258 community dwelling women from urban and rural settings who participated in health campaigns. In order to reduce the sampling bias, half of the study population performed the self-sampling prior to the physician sampling while the other half performed the self-sampling after the physician sampling, randomly. Acquired samples were assessed for cytological changes as well as HPV DNA detection. Results: The mean age of the subjects was $40.4{\pm}11.3years$. The prevalence of abnormal cervical changes was 2.7%. High risk and low risk HPV genotypes were found in 4.0% and 2.7% of the subjects, respectively. A substantial agreement was observed between self-sampling and the physician obtained sampling in cytological diagnosis (k=0.62, 95%CI=0.50, 0.74), micro-organism detection (k=0.77, 95%CI=0.66, 0.88) and detection of hormonal status (k=0.75, 95%CI=0.65, 0.85) as well as detection of high risk (k=0.77, 95%CI=0.4, 0.98) and low risk (K=0.77, 95%CI=0.50, 0.92) HPV. Menopausal state was found to be related with 8.39 times more adequate cell specimens for cytology but 0.13 times less adequate cell specimens for virological assessment. Conclusions: This study revealed that self-sampling has a good agreement with physician sampling in detecting HPV genotypes. Self-sampling can serve as a tool in HPV screening while it may be useful in detecting cytological abnormalities in Malaysia.

• Convergence Security Journal
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• v.17 no.1
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• pp.89-100
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• 2017

This study has an objective to analyze the causal relationship between the business resource of the members in the Airport Special Guard's firms and its effect on their business attitude and turnover intention. To carry out the study, I established a population of Airport Special Guard's working at the Airport firms in In-cheon, Kim-po, Cheong-ju and Dae-gu. Then a total of 300 questionnaires were sent out for the survey using the random sampling method of clusters. The study was carried out over about four months, from July, 2016 to october, 2016. A total of 260 questionnaires were used for the final data. 40 questionnaires were excluded either because they were not returned or contained extreme value. In this study, a statistical package of SPSS WIN 18.0 were used in the data analysis and the causal relationship was verified using the multiple regression analysis. The analysis showed the business resource to have a positive effect on the business attitude but a negative effect statistical level of p<.01 on the turnover intention at the statistical level of p<.001 and the hypothesis was adopted. The hypothesis regarding the business attitude was also adopted because business attitude was found to have a negative effect on the turnover intention at the statistical level of p<.01. In addition, business attitude was found to have a negative effect on the relationship between the business resource and turnover intention at the statistical level of p<.05. As a result, the hypothesis about the business attitude would function as the parameter was only partially adopted. Through these research result, it was investigated that the relation between business resource, business attitude and turnover intention is significantly effective variable in airport special guard's. Additionally, business resource is the most important.

• Korean Journal of Environment and Ecology
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• v.30 no.6
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• pp.939-961
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• 2016

For effective conservation of endangered wild plants, it is necessary to understand their interactions with environmental factors in each habitat together with life traits of target species. First, the characteristics of their distribution were investigated followed by their monitoring for 4 years focusing on the habitats in the lagoon. Also, their life traits were compared including production of hibernacles, fruits, and seeds by the soil fertilization and light intensities. Next, the information on the species was secured by germination experiment using the generated seeds from the cultivation experiment. The habitat of Glaux maritima var. obtusifolia Fernald in Korea was located in the rear edge of the worldwide distribution and its four habitats were isolated and distributed far away each other. Two of them were located in small salt-marsh and fine sand estuaries formed in the rocky area of the seashore, and the other two were inhabited with the sandy soil in the lagoon which was connected by river-mouth to the sea. Glaux maritima var. obtusifolia Fernald tends to be distributed in the sites where the establishment and growth of the competitor were inhibited by salinity, periodic flooding, and lower layer of the soil to extend a roots. It maintained its population by recruitments of hibernacles and seedling. The production of hibernacle was assumed to be affected by the particle consist of the sand together with organic matters in the soil. Seedling recruitment was observed only in the salt-marsh area located in the rear sites of sand ridge where was the shore of the lagoon. Glaux maritima var. obtusifolia Fernald was observed to have different threatening factors by each population. Its population in Pohang seemed the sedimentation of fine sand which affected the recruitment of hibernacles had been eroded due to the construction of the coastal road. The population in Ulsan appeared rapid expansion of competitor and reduction of its distribution area due to the interruption of eluted water supplied to the habitat. On the other hand, the habitat in the lagoon maintained the population relatively stable. Especially, the population in Songji-ho was determined to be the most stable one. To sustain the population of Glaux maritima var. obtusifolia Fernald distributed in the lagoon, it is suggested that the wide ranged scale of conservational activities is necessary to maintain the mechanisms including the entrance of seawater which belongs to the lagoon, and periodic flooding.

• The Korean Journal of Ecology
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• v.17 no.3
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• pp.299-310
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• 1994

Intraspecific clonal interactions have important influences on a population structure of the cellular slime mold (CSM). This study was to investigate whether or not evolutionary change in a population could be induced by clonal competition, and to elucidate how various clones in a population evolve in a homogeneous environment of laboratory culture. The characteristic clones of Polysphondylium pallidum which had different resource consumption rates (RCR) and mating types I and II were selected for study. Investigation was conducted for 4 experimental time interval $(T_0-T_4)$; one experimental time interval took almost 10-14 days from inoculation to havest of fruiting bodies. Two sets of 50 clones were cultured from 50 clones at To, and RCR variations of the population were compared between $(T_0\;and\;T_4)$ for each set of clones. Each clone of the CSM had a diverse resource consumption rate, or growth rate, in a homogeneous and limited Cerophyl agar plate despite the passage of 48-56 generations from the beginning of the experiment. Diverse clones with different growth rate could coexist in one site of the homogeneous agar plate as well as heterogeneous soil microenvironment. When there was high clonal diversity of RCR, a clone in a population had high chances to encounter other clones with resultant increased clonal competition. In one set, 26 of 37 clones of mating type I were changed to mating type Il for the 4 experimental time intervals, which indicated that the rate of competitive exclusion among clones during total experiment from $(T_0\;to\;T_4)$ was 0.703. In another set, 31 of 37 clones of mating type I were changed to mating type II , having the rate of competitive exclusion 0.838. The frequency of each of mat~ng types changed by 0.93-1.29% in each successive generation. The competitive exclusion among clones occurred by 1.26-1.75% when approximately $2.6{\times}10^8$ bacterial cells were provided as food and thereafter one generation of myxamoebae of CSM elapsed at room temperature. This finding implicated that in the vegetative state of P, pallidurn there was 1.26-1.75% probabil~ty of evolutionary change per generation changing from one clone to another clone.

• Animal Bioscience
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• v.34 no.4
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• pp.525-532
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• 2021

Objective: Old World camels are a valuable genetic resource for many countries around the world due to their adaptation to the desert environment. At present, Old World camels have encountered the challenge of unprecedented loss of genetic resources. Through our research, we would reveal the population structure and genetic variation in Old World camel populations, which provides a theoretical basis for understanding the germplasm resources and origin and evolution of different Old World camel populations. Methods: In the present study, we assessed mtDNA control region sequences of 182 individuals from Old World camels to unravel genetic diversity, phylogeography, and demographic dynamics. Results: Thirty-two haplotypes confirmed by 54 polymorphic sites were identified in the 156 sequences, which included 129 domestic and 27 wild Bactrian camels. Meanwhile, 14 haplotypes were defined by 47 polymorphic sites from 26 sequences in the dromedaries. The wild Bactrian camel population showed the lowest haplotype and nucleotide diversity, while the dromedaries investigated had the highest. The phylogenetic analysis suggests that there are several shared haplotypes in different Bactrian camel populations, and that there has been genetic introgression between domestic Bactrian camels and dromedaries. In addition, positive values of Tajima's D and Fu's Fs test demonstrated a decrease in population size and/or balancing selection in the wild Bactrian camel population. In contrast, the negative values of Tajima's D and Fu's Fs test in East Asian Bactrian camel populations explained the demographic expansion and/or positive selection. Conclusion: In summary, we report novel information regarding the genetic diversity, population structure and demographic dynamics of Old World camels. The findings obtained from the present study reveal that abundant genetic diversity occurs in domestic Bactrian camel populations and dromedaries, while there are low levels of haplotype and nucleotide diversity in the wild Bactrian camel population.

• Journal of Korean Society of Disaster and Security
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• v.14 no.4
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• pp.61-67
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• 2021

In this study, the adequacy of water supply critical factors of urban design was examined. The supply of water supply is analyzed in various ways as the design progresses. Starting with basic data collection and analysis a supply and demand plan is established to calculate the amount of water supply and in this study the adequacy of population estimation and original unit calculation was evaluated. Among the second new cities where actual data can be secured Wirye New Town was selected as the study target area. Related data were analyzed to confirm the future population and the original unit and compared with the measured data. As of September 2020, the population of Wirye New Town was 93,977, showing the appropriateness of about 84% with a planned population of 110,990 confirming that the planned population and the actual population were almost similar. In the case of the original unit, it was calculated as 314 liters per person in Seoul and 320 liters per person in Seongnam at the time of design. As a result, it was found that there was some agreement in the population estimation while examining the supply in the planned city. In the case of Korea, there is a lot of interest in revitalizing the existing city, away from continuous development. Therefore it is judged that there is a need for further research on the adequacy of supply for the old city center.

• Journal of Family Resource Management and Policy Review
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• v.16 no.1
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• pp.123-148
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• 2012

The main objective of this study was to investigate how people prepare for old age by looking at financial statements from single-person households in Gyeonggi province; the policy implications regarding low fertility and population aging are considered. The subjects were members of 600 households who were aged 26 to 44 years old and not married. The data were analyzed by t-test, ANOVA, and dichotomous logistic regression using SPSS WIN 12.0. Respondents pointed out that financial problems are the greatest challenges for those facing old age in single-person households; they said that a stable job is the most important factor for secure living. About 57% of household residents responded that they have prepared for retirement, and a majority of them said they hoped to spend their elderly years with a marriage partner. The financial statements from single-person households were inferior to those from other types of households. The assets of single-person household members were less than those of other households. Only 10.5% of respondents have owned homes. The study discusses policy implications for those who want marriage and for those who don't want it. For those who want marriage, job-seeking support was proposed, and for the others, tax deductions and family programs for single-person households were proposed.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.4
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• pp.482-485
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• 2006

Korean native goats have lived on the Korean peninsula for more than 2,000 years and are regarded as a valuable genetic resource for the world. As an initial step to investigate the genetic structures of this breed, phylogenetic analysis and calculation of genetic diversities have been performed using mitochondrial DNA (mtDNA) sequence variations. A total of 19 Korean native goats were grouped into six haplotypes and the large majority of haplotypes were present in 13 animals. All mtDNA of these Korean goats belonged to the mitochondrial (mt) lineage A and revealed remarkably small genetic distances within the population when compared with other Asian goat populations, indicating less genetic variation in the Korean native goats. These results indicate high-inbred status of the Korean native goats and will influence breeding and conservation strategies adopted for this breed.