• Title/Summary/Keyword: Resistant variety

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Feasibility in Differentiation of Resistance of Rice Varieties to Brown Planthopper (Nilaparvata lugens Stal) using Radisoisotope (P-32) Tracer-Technique (P-32를 이용한 벼멸구(Nilaparvata lugens Stal) 저항성 검정법에 관한 연구)

  • Chung K.H.;Kwon S.H.;Choi S.Y.
    • Korean journal of applied entomology
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    • v.20 no.4 s.49
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    • pp.207-211
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    • 1981
  • It has been documented that the resistance to planthoppers is attributed to the feeding preference of the insects. This might be related to difference in the amount of feeding plant sap between resistant and susceptible hosts. In this aspect, this study was conducted to verify it and to develop an effective screening method for resistance to planthoppers by tracer technique. An effective P-32 labelling of rice seedlings at $2\~3$ leaf stage was dipping the roots in concentration of $0.1{\mu}Ci/ml$ solution for 48 hours. Radioactivity was significantly higher in planthopers fed on susceptible variety for 48 hours as compared to those fed on resistant variety. Radioactivity of adults was higher than that of nymphs and also higher in female than male. The results were highly correlated to that indicated by the feeding preference of the insects and therefore, considered to be valid for a screening technique.

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Evaluation of Phytophthora Late Blight Resistance of Potato Variety in Korea (국내에서 육성한 감자품종의 역병 저항성평가)

  • Ryu, Kyoung-Yul;Jee, Hyeong-Jin;Shim, Chang-Ki;Lee, Byung-Mo;Park, Jong-Ho;Choi, Du-Hoe;Ryu, Gab-Hee;Park, Young-Eun
    • Research in Plant Disease
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    • v.12 no.3
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    • pp.235-239
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    • 2006
  • Since the cultivation of resistance cultivar is essential for organic agriculture, Phytophthora late blight resistance of 16 advanced potato clones obtained from the potato breeding laboratory at the National Institute of Highland Agriculture was evaluated. Resistance of the clone was examined through artificial inoculation in the laboratory and in the field. Dominant isolates of the pathogen in Gangwon province produced abundant sporangia on leaves of most clones showing susceptibility. The number of sporangia ranged from $10^{7{\sim}8}sporangia/ml$ in the susceptible clones at 7 days after inoculation. However, one clone is resistant in organic farming fields. Disease incidence was 2.3% using the resistant clone. The area under disease progress curve(AUDPC) was 75.5. Contrarily, disease incidence of the susceptible variety was 100% and the AUDPC was 1773.5 during the same cultivation period. The resistance clone named as 'Haryeong' is considered suitable for organic potato cultivation in Gangwon province in Korea.

The Agronomic Characteristics of the Yongcheonppong (Morus spp.) Appropriate for the Cold Region where the Freezing Injury Occured and Consideration on the Result of the Silkworm Rearing Affected by the Occured Frequency of the Freezing Injury (凍害常習地 적응 뽕품종 “龍川뽕”의 재배학적 특성 및 凍害 發生頻度에 따른 蠶作狀況의 몇가지 考察)

  • 박광준;조장호;남학우;성규병
    • Journal of Sericultural and Entomological Science
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    • v.44 no.1
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    • pp.22-27
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    • 2002
  • The Yongcheonppong was cultured in the form of a dense mulberry field coverd with polyethylene film and was tested in Suwon and Chunchon. The agronomic characteristic and some consideration on the result of the silkworm rearing were as follows. The Yongcheonppong was the medium budding variety and was longer in length of young shoots than the Kaeryangppong. The Yongcheonppong was higher in leaf yield in spring than the Kaeryangppong and was lower in leaf yield in autumn than the Kaeryangppong to the contrary. The Yongcheonppong was a little higher in the number of branches and was longer in the length of the branch than the Kaeryangppong and so was appropriate for rearing with mulberry shoots. Yongcheonppong was more resistant to twig blight and die-back and was a little susceptible to dwarf disease. As the Yongcheonppong was most resistant to the freezing injury, it was appropriate for the cold region and enabled the farmers to rear some amount of silkworms every year. In Chunchon, The Yongcheonppong increased by 11% in cocoon yield than the Kaeryangppong when the freezing injury occurred 1 year out of 2 years and produced about the same as the Kaeryangppong in cocoon yield when the freezing injury occurred 1 year out of 3 years.

A Study on the Image Analysis Technique for the Precision Exploration of Chili Anthracnose (고추 탄저병 정밀 탐색을 위한 영상분석 기술에 대한 연구)

  • Baek, JeongHo;Kim, Nyunhee;Lee, Eungyeong;Lee, Hongseok;Kim, Song Lim;Park, Sang Ryeol;Ji, Hyeonso;Choi, Inchan;Kim, Kyung-Hwan
    • Journal of Korea Society of Industrial Information Systems
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    • v.25 no.5
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    • pp.73-81
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    • 2020
  • One of the most important vegetables consumed in Korea, chili peppers (Capsicum annuum) are widely cultivated around the world. Chili peppers have been severely damaged by anthracnose during their growth, so it is important to study prevention and resistance varieties. K1 anthracnose was inoculated against four cultivar of chili peppers that are resistant to anthracnose and one cultivar that is sensitive. The area of the disease that appeared over time was photographed and quantified through the program was analyzed. Through the ratio of the area of chili pepper fruit and the area of the bottle, the sensitive variety An-S showed weak reactions to anthracnose with about 40%, the resistant variety An-12R (23%), An-Tan (21%), and An-9R (19%), and PBC81 showed a strong response to anthracnose with about 11%. These quantitative value can be used as a basis for comparison in conducting resistance studies for new varieties.

Comparison of Forage Productivity and Outbreak Rate Between Corn Varieties in Rice Black-Streaked Dwarf Virus (RBSDV) Prevalent Area (검은줄오갈병 발생이 심한 지역에서 옥수수 품종의 발병률과 사초 생산성 비교)

  • Choi, Gi Jun;Lim, Young Chul;Yoon, Sei Hyung;Ji, Hee Chung;Lee, Sang-Hoon;Jung, Min Woong;Seo, Sung;Park, Hyung Soo;Kim, Ki-Yong
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.33 no.2
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    • pp.111-116
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    • 2013
  • This experiment was carried out to select a resistant corn variety for rice black-streaked dwarf virus (RBSDV) disease in a RBSDV prevalent area (Gochang of Jeollabukdo) from 2006 to 2008. Ten corn varieties for silage preparation were cultivated with first cropping and second cropping system in an RBSDV field, and were tested outbreak rates of RBSDV and dry matter (DM) yield of forage. The outbreak rates of RBSDV were significantly different between corn varieties. Therefore, the resistance degree of corn varieties for RBSDV were divided into 4 groups; very resistant ('Kwangpeyongok' and 'Kwanganok'), resistant ('P3156' and 'P3394'), mildly resistant ('Cheonganok' and 'P32P75') and susceptible ('Suwon19', 'DK697' and 'GW6959') groups respectively. DM yield of forage in RBSDV fields exhibited comparatively significant differences between varieties (p<0.05). DM yield of resistant varieties ('Kwangpeyongok', 'Kwanganok', 'P3156' and 'P3394'), were higher (14~26%) than those of susceptible varieties ('Suwon 19', 'DK697' and 'GW6959'). Therefore, resistant varieties were recommended for increasing forage productivity in field of RBSDV prevalent areas.

Repellent and Insecticidal Activity of Sequential Extracting Fractions Obtained from BPH-Resistant Rice Varieties against Brown Planthopper (Nilaparvata lugens) (벼멸구 저항성벼 품종 추출분획물의 기피 및 살충 활성)

  • Kim, Sung-Eun;Kim, Young-Doo;Kim, Bo-Kyoung;Ko, Jae-Kwon;Chun, Jae-Chul
    • The Korean Journal of Pesticide Science
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    • v.10 no.2
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    • pp.124-130
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    • 2006
  • Rice plant extracts of brown planthopper (BPH) resistant rice varieties, Jangseongbyeo (JSB) and Hwacheongbyeo (HCB) at different growth stages (seedling, tillering, heading and ripening) were sequentially fractioned using hexane, ethyl ether, ethyl acetate, butanol, and distilled water. The extracts were applied to BPH susceptible rice variety, Dongjjnbyeo (DJB), to investigate the insecticidal and repellent effects against BPH. BPH insecticidal effects were not clearly observed with almost all of the extract fractions obtained from both JSB and HCB varieties for 12 h, whereas the ethyl ether and hexane extract fractions showed about 10 to 30% of BPH mortality in 24 to 48 h of application periods. An effective BPH repellent activity was found with the applications of ethyl ether extract fractions obtained from JSB variety. The extract fractions obtained from HCB variety did not show any different repellence among the various fractions. The BPH repellent effects of the extract fractions obtained at different growth stages of either JSB or HCB varieties did not show any correlations. The effect of ethyl ether fraction on BPH repellent was continually increased by 30 h after treatment and thereafter decreased. In addition, the first sub-fraction separated by a flash column chromatography eluted with chloroform:methanol (9:1, v/v) from the BPH effective ethyl ether faction in JSB variety might be meaningful to repel BPH from BPH susceptible target rice plants. The results indicated that the ethyl ether fraction obtained from JSB was higher in repellent activity than in insecticidal activity, and suggesting that there might be specific substance(s) in the first sub-fraction (sF1) of the ethyl ether fraction in JSB that could provide repellent activity against BPH.

Antibacterial Activities of Fermented Sayuksan Ingredient Extracts for Multidrug-resistant Strains (한약재발효액의 항생제 다제내성균에 대한 항균활성 및 항산화활성)

  • Park, Young-Ja;Kang, Dong Hee;Kim, Hyun-Soo
    • KSBB Journal
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    • v.29 no.3
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    • pp.210-219
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    • 2014
  • Sayuksan has been widely applied to treat a variety of diseases such as acute hepatitis, gastritis, and colitis. Sayuksan consists of medicinal herbs such as Glycrrhizae uralensis Fischer, Paeonia lactiflora Pallas, Bupleurum falcatum Linne, and Poncirus trifoliata Rafinesqul. Methanol extracts (1 mg/mL) from the four kinds of medicinal herbs did not show antibiotic activities against general test strains and multi-drug resistant strains. The antibacterial activity of fermented medicinal herbs extracts with Lactobacillus spp. strain was confirmed as Gram-positive bacteria which are higher than Gram-negative bacteria. Extracts of Glycrrhizae uralensis Fischer fermented with Lb. casei KCTC 3109 displayed inhibitory diameters of 16 mm against Pseudomonas aeruginosa P01828. Superoxide dismutase (SOD)-like activity of the medicinal herb extracts was not determined, but the extract of Paeonia lactiflora Pallas fermented with six strains of Lactobacillus spp. had the highest antioxidant activity. SOD-like activity of Paeonia lactiflora Pallas extracts fermented by Lb. brevis KCTC 3498 was $41.4{\pm}0.8%$, which was the highest antioxidant activity among the fermented extracts with the other medicinal herbs.

Virulence Factors and Antimicrobial Resistance of Vibrio parahaemolyticus Isolated from Commercial Fisheries Products (시판수산물에서 분리한 Vibrio parahaemolyticus의 병원성 인자와 항균제 내성 현황)

  • Lee, Ye Ji;Kim, Eunheui
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.52 no.6
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    • pp.596-604
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    • 2019
  • Vibrio parahaemolyticus causes food poisoning, mainly via marine fisheries products. We investigated the virulence factors and drug resistance of V. parahaemolyticus isolated from fisheries products purchased from the Yeosu Fisheries Market. The isolates were identified using a variety of biochemical tests and the detection of toxR and hns gene. The presence of the virulence factor-encoding genes tdh and trh in the isolates was also investigated by PCR. The resistance of the isolates to 13 antibacterial agents was tested using the disc-diffusion method and carriage of β-lactamase genes and class 1 integrons by ampicillin-resistant isolates was investigated by PCR. Four of seventeen isolates identified as V. parahaemolyticus by biochemical tests produced a species-specific PCR band. Those isolates showed >98% 16S rRNA gene sequence homology with V. parahaemolyticus and only one isolate harbored the tdh gene. All of the V. parahaemolyticus isolates were resistant to ampicillin and amoxicillin; moreover, VPA0477, a class A β-lactamase gene, and class 1 integrons were detected. Therefore, V. parahaemolyticus from fisheries products represents a low risk to human health. Also, V. parahaemolyticus is likely to develop multidrug resistance because it has class 1 integrons.

Ultraviolet-B radiation sensitivities in rice plant: cyclobutane pyrimidine dimer photolyase activities and gene mutations

  • Hidema, Jun;Kumagai, Tadashi
    • Proceedings of the Korean Society of Plant Biotechnology Conference
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    • 2004.10a
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    • pp.29-34
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    • 2004
  • Reduction in stratospheric ozone layer increases the amount of ultraviolet-B radiation (UVB: 280-320 nm) that reaches the earth ’ s surface. UVB radiationcan damage plants, resulting in decrease in growth and productivity. UVB-augmentation studies have indicated that the sensitivity to UVB radiation in plants varies among the species and cultivars. However. there are no definitive answers for the mechanisms of UVB-resistance in higher plants and for bioengineering design and development of UVB-tolerant plants. We have been studying physiological and biochemical aspects of the effects of UVB radiation on growth and yield of rice COryza sativa LJ. aiming to clarify the mechanism of resistance to UVB radiationin rice. At this meeting. weintroduce our research as followed: (1) supplementary UVB radiation has inhibitory effects on the growth. yield and grain development of rice; (2) UVB sensitivity of rice varies widely among cultivars; (3) among Japanese rice cultivars. Sasanishiki. a leading variety in northeast Japan. is more resistant to UVB. while Norin 1. a progenitor of Sasanishiki. is less resistant; (4)UV-sensitive Norin 1 cultivar is deficient in photorepair of UVB-induced cyclobutane pyrimidine dimer (CPD). and this deficiency results from one amino acid residue alteration of CPD photolyase. These results suggest that spontaneously occurring mutation in CPD photolyase gene could lead to difference in UVB sensitivity in rice. and that CPD photolyase might be a useful target for improving UVB-sensitivity in rice by selective breeding or bioengineering of UVB-tolerant rice.

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Biocompatibility of STS304, 316 acupuncture needle (침의 재료인 스테인리스강 STS304 및 STS316의 생체적합성에 대한 고찰)

  • Hwang, Eui-Hyoung;Koo, Sung-Tae;Lee, In-Hwan;Lee, Min-Ho;Jang, In-Soo
    • Korean Journal of Acupuncture
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    • v.24 no.1
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    • pp.59-63
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    • 2007
  • Objectives : To discuss the biocompatibility of stainless steel for using acupuncture needles. Results and Conclusions : It is widely assumed that stainless steel is one of the good corrosion-resistant materials and is safe for human body, Most of metal acupuncture needles are made of STS304 and STS316 in light of human body safety: STS304 in more differences between STS304 and STS316 in light of human body safety: STS304 is more vulnerable to oxidation than STS316. A variety of literature survey showed that although STS316 is more corrosion-resistant than STS304 there is no evidence supporting the hypothesis that STS304 has the potential danger to human body, Further study would be necessary to draw more decisive conclusion.

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