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Repellent and Insecticidal Activity of Sequential Extracting Fractions Obtained from BPH-Resistant Rice Varieties against Brown Planthopper (Nilaparvata lugens)  

Kim, Sung-Eun (Honam Agricultural Research Institute, NICS, RDA)
Kim, Young-Doo (Yeongnam Agricultural Research Institute, NICS, RDA)
Kim, Bo-Kyoung (Honam Agricultural Research Institute, NICS, RDA)
Ko, Jae-Kwon (Honam Agricultural Research Institute, NICS, RDA)
Chun, Jae-Chul (Research Center of Bioactive Materials, Chonbuk National University)
Publication Information
The Korean Journal of Pesticide Science / v.10, no.2, 2006 , pp. 124-130 More about this Journal
Abstract
Rice plant extracts of brown planthopper (BPH) resistant rice varieties, Jangseongbyeo (JSB) and Hwacheongbyeo (HCB) at different growth stages (seedling, tillering, heading and ripening) were sequentially fractioned using hexane, ethyl ether, ethyl acetate, butanol, and distilled water. The extracts were applied to BPH susceptible rice variety, Dongjjnbyeo (DJB), to investigate the insecticidal and repellent effects against BPH. BPH insecticidal effects were not clearly observed with almost all of the extract fractions obtained from both JSB and HCB varieties for 12 h, whereas the ethyl ether and hexane extract fractions showed about 10 to 30% of BPH mortality in 24 to 48 h of application periods. An effective BPH repellent activity was found with the applications of ethyl ether extract fractions obtained from JSB variety. The extract fractions obtained from HCB variety did not show any different repellence among the various fractions. The BPH repellent effects of the extract fractions obtained at different growth stages of either JSB or HCB varieties did not show any correlations. The effect of ethyl ether fraction on BPH repellent was continually increased by 30 h after treatment and thereafter decreased. In addition, the first sub-fraction separated by a flash column chromatography eluted with chloroform:methanol (9:1, v/v) from the BPH effective ethyl ether faction in JSB variety might be meaningful to repel BPH from BPH susceptible target rice plants. The results indicated that the ethyl ether fraction obtained from JSB was higher in repellent activity than in insecticidal activity, and suggesting that there might be specific substance(s) in the first sub-fraction (sF1) of the ethyl ether fraction in JSB that could provide repellent activity against BPH.
Keywords
brown planthopper; BPH resistant rice variety; chemical extract fractions; insecticidal effect; repelling activity;
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